Home DNA Damage/DNA Repair DNA/RNA Synthesis inhibitor SCR130 For research use only.

SCR130

SCR130 is a novel DNA repair inhibitor with IC50s of 14 μM in Reh cells and 2 μM in Nalm6 cells. SCR130 specifically inhibits DNA Ligase IV‐mediated joining with minimal or no effect on Ligase III and Ligase I mediated joining.

SCR130 Chemical Structure

SCR130 Chemical Structure

CAS: 2377858-38-1

Purity & Quality Control

Batch: Purity: 99.08%
99.08

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Signaling Pathway

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Biological Activity

Description SCR130 is a novel DNA repair inhibitor with IC50s of 14 μM in Reh cells and 2 μM in Nalm6 cells. SCR130 specifically inhibits DNA Ligase IV‐mediated joining with minimal or no effect on Ligase III and Ligase I mediated joining.
Targets
DNA repair [1]
(in Nalm6 cells)
2 μM
In vitro
In vitro

SCR130 is a new a novel inhibitor of nonhomologous end-joining (NHEJ) by leading to loss of mitochondrial membrane potential leading to cell death by activating both intrinsic and extrinsic pathways of apoptosis, showing the IC50 of 14.1 μM in Reh cells and 2.2 μM in Nalm6 cells.<sup><a class="sref" href="#s_ref">[1]</a></sup>
Cell Research Cell lines Reh, HeLa, CEM, Nalm6 and N114 cells
Concentrations 0.1, 0.5, 1, 2, 5, 10, 50, and 100 μM
Incubation Time 48 h
Method

Effect of the inhibitors on Nalm6, Reh, HeLa, CEM, and N114 cell lines was determined by Trypan Blue dye exclusion assay

Chemical Information & Solubility

Molecular Weight 418.30 Formula

C19H13Cl2N3O2S
CAS No. 2377858-38-1 SDF --
Smiles ClC1=CC=C(C=C1)C2CC(=NC23C(=O)NC(=S)NC3=O)C4=CC=C(Cl)C=C4
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 84 mg/mL ( (200.81 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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