Sarsasapogenin

Catalog No.S3607 Batch:S360701

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Technical Data

Formula

C27H44O3
Molecular Weight 416.64 CAS No. 126-19-2
Solubility (25°C)* In vitro Ethanol 5 mg/mL (12.0 mM)
DMSO Insoluble
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Sarsasapogenin (SAR, Parigenin) is a steroidal sapogenin. It can provoke the generation of reactive oxygen species and activate unfolded protein response (UPR) signaling pathways. This compound potently inhibits NF-κB and MAPK activation, as well as IRAK1, TAK1, and IκBα phosphorylation in LPS-stimulated macrophages.
Targets
NF-κB [2] IRAK1 [2] TAK1 [2] IκBα [2]
In vitro Sarsasapogenin induces an increase in the population of Hela cells in S phase and an obvious accumulation of cells in G2/M phase in a time-dependent manner. It induces apoptosis in HeLa cells via the caspase-dependent mitochondrial apoptotic pathway. This compound induces the activation of ER stress pathway. It induces cytotoxic effects in human cervical cancer cells via cell cycle arrest, ROS-mediated mitochondrial pathway and ER stress pathway. This chemical induces the activation of UPR, the ER specific stress response at early stage and then activates CHOP, which may contribute to the initiation and augment of mitochondrial membrane permeabilization by dephosphorylation of Akt so as to mediate the apoptotic signals from ER to mitochondria[1]. It potently inhibits NF-κB and MAPK activation, as well as IRAK1, TAK1, and IκBα phosphorylation in LPS-stimulated macrophages. This compound inhibits the binding of LPS to macrophage Toll-like receptor 4, as well as polarization of M2 to M1 macrophages[2].
In vivo Oral administration of sarsasapogenin inhibits 2,3,4-trinitrobenzene sulfonic acid (TNBS)-induced colon shortening and myeloperoxidase activity in mice, along with reducing NF-κB activation and interleukin (IL)-1β, tumor necrosis factor (TNF)-α, and IL-6 levels, while simultaneously increasing IL-10. This compound inhibits Th17 cell differentiation in colonic lamina propria, but induces Treg cell differentiation. It potently inhibits inflammatory responses in vivo[2].

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    Hela cells

  • Concentrations

    0-60 μM

  • Incubation Time

    48 h

  • Method

    HeLa cells are incubated with sarsasapogenin (0-60 μM) for 48 h. Hoechst 33342-stained nuclei are visualized using confocal laser scanning microscopy.
Animal Study:

[2]
  • Animal Models

    C57BL/6 mice

  • Dosages

    5 or 10 mg/kg

  • Administration

    oral

References

  • https://pubmed.ncbi.nlm.nih.gov/24513212/
  • https://pubmed.ncbi.nlm.nih.gov/25698557/

Selleck's Sarsasapogenin Has Been Cited by 1 Publication

A positive feedback loop between gastric cancer cells and tumor-associated macrophage induces malignancy progression [ J Exp Clin Cancer Res, 2022, 41(1):174] PubMed: 35562774

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.