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PH-002

PH-002 is an inhibitor of Apolipoprotein E4 (ApoE4) intracellular domain interaction in nerve cells.

PH-002 Chemical Structure

PH-002 Chemical Structure

CAS: 1311174-68-1

Purity & Quality Control

Batch: S008201 DMSO] 98 mg/mL] false] Ethanol] 25 mg/mL] false] Water] Insoluble] false Purity: 99.10%
99.10

PH-002 Related Products

Biological Activity

Description PH-002 is an inhibitor of Apolipoprotein E4 (ApoE4) intracellular domain interaction in nerve cells.
In vitro
In vitro

PH-002 is the small-molecule structure corrector for apolipoprotein (apo) E4, which can disrupt domain interaction in Neuro-2a cells and rescue the impaired intracellular trafficking of apoE4.[1]
Cell Research Cell lines Wild type Neuro-2a neuroblastoma cells
Concentrations 100 nM
Incubation Time 72 h
Method

Neuro-2a cells are transfected withEGFP-apoE3, EGFP-apoE4, and EGFP-apoE4-R61T using Lipofectamine 2000 and selected with 400 μg/ml neomycin for 4 weeks. Colonies with green fluorescence are picked for further enrichment. Neuro-2a cells are seeded at 7500-8000 cells/well on 24-well plates containing Opti-MEM with DMSO plus 100 nM PH002 for 72 h. Cells are plated 2 days before the FRAP measurement experiment in eight-well chamber slides with a coverglass bottom coated with PL-lysine at 5000 cells/well. Recordings are obtained with an inverted Leica SP5 confocal microscope and a 63×oil objective with a numerical aperture of 1.4; cells are maintained at 37 ℃ in 5% CO2.

Chemical Information & Solubility

Molecular Weight 491.58 Formula

C27H33N5O4
CAS No. 1311174-68-1 SDF --
Smiles CC(C)(C)OC(=O)N1CCN(CC1)CC2=CC=C(C=C2)NC(=O)CC3=NN(C(=O)C4=CC=CC=C43)C
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 98 mg/mL ( (199.35 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 25 mg/mL

Water : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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