Home DNA Damage/DNA Repair DNA/RNA Synthesis activator - Apoptosis related activator - Autophagy activator PCNA-I1 For research use only.

PCNA-I1

PCNA-I1 is a selective inhibitor of proliferating cell nuclear antigen (PCNA, a potential anticancer target). PCNA-I1 selectively binds to PCNA trimers with Kd of ~0.2 to 0.4 μM. PCNA-I1 inhibits the growth of tumor cells of various tissue types with IC50 of ~0.2 μM. PCNA-I1 induces DNA damage and apoptosis in both LNCaP and PC-3 cells. PCNA-I1 also induces autophagy in PC-3 cells.

PCNA-I1 Chemical Structure

PCNA-I1 Chemical Structure

CAS: 444930-42-1

Purity & Quality Control

Batch: Purity: 99.97%
99.97

PCNA-I1 Related Products

Signaling Pathway

DNA/RNA Synthesis Signaling Pathways

DNA/RNA Synthesis Signaling Pathway

Choose Selective DNA/RNA Synthesis Inhibitors

Biological Activity

Description PCNA-I1 is a selective inhibitor of proliferating cell nuclear antigen (PCNA, a potential anticancer target). PCNA-I1 selectively binds to PCNA trimers with Kd of ~0.2 to 0.4 μM. PCNA-I1 inhibits the growth of tumor cells of various tissue types with IC50 of ~0.2 μM. PCNA-I1 induces DNA damage and apoptosis in both LNCaP and PC-3 cells. PCNA-I1 also induces autophagy in PC-3 cells.
Targets
PCNA trimers [1]
(Cell-free assay)
0.2 to 0.4 μM(Kd)
In vitro
In vitro

PCNA-I1 prevents the transportation of the Proliferating Cell Nuclear Antigen (PCNA) to the cytoplasm by stabilizing its trimer form in the nucleus, thus inhibits SARS-CoV-2 replication.[3]
Cell Research Cell lines Vero E6 cells
Concentrations 0.1, 1, 10 and 100 µM
Incubation Time 96 h
Method

Lung tissue sections A plaque assay was performed. Briefly, after seeding, Vero E6 (8 × 105 cells per well, 6-well plates) were incubated overnight and infected with 200 PFU per well. PCNA-I1 or Verdinexor were added at final concentrations of 0.5 µM or 0.1 µM and 0.1 µM or 1 µM, in overlays composed of DMEM supplemented with 10% FBS plus carboxymethylcellulose sodium salt 2%, immediately post-viral adsorption. A MTT cell proliferation assay was done to verify the toxicity of the drugs. After four days, cells were fixed with formaldehyde and stained with crystal violet to count plaques.

Chemical Information & Solubility

Molecular Weight 310.37 Formula

C17H14N2O2S
CAS No. 444930-42-1 SDF --
Smiles CC1=C(SC=C1)C(=O)N\N=C\C2=C(O)C3=CC=CC=C3C=C2
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 62 mg/mL ( (199.76 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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