Oxolinic acid

Catalog No.S4537 Synonyms: Urinox, NSC 110364

For research use only.

Oxolinic acid (Urinox, NSC 110364) is a quinolone antibiotic, inhibiting the enzyme DNA gyrase and DNA synthesis.It also acts as a dopamine reuptake inhibitor.

Oxolinic acid Chemical Structure

CAS No. 14698-29-4

Purity & Quality Control

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Biological Activity

Description Oxolinic acid (Urinox, NSC 110364) is a quinolone antibiotic, inhibiting the enzyme DNA gyrase and DNA synthesis.It also acts as a dopamine reuptake inhibitor.
Targets
bacterial DNA gyrase [3] DNA synthesis [4] dopamine uptake [2]
4.3 μM
In vitro

Oxolinic acid, a quinolone antibacterial agent, inhibits reversibly the ATP-dependent replicative DNA synthesis in permeable cell systems as well as in cellophane disk lysates[4]. It also inhibits bacterial DNA gyrase but not eukaryotic topoisomerases, reversibly binding gyrase subunit A in gyrase-DNA complexes, blocking supercoiling activity and inhibiting DNA synthesis at 0.5-5 µg/ml[5][6][7].

In vivo The antimicrobial agent oxolinic acid, injected i.p. in mice, induces a dose dependent increase in locomotor activity. the stimulant locomotor effect of oxolinic acid depends on the blockade of the neuronal dopamine uptake complex[2].

Protocol (from reference)

Animal Research:

[1]

  • Animal Models: Mice(ICR mice), rats( Sprague-Dawley rats), and beagle dogs
  • Dosages: 20, 50, and 100 mg/kg
  • Administration: By gavage

Solubility (25°C)

In vitro

DMSO 0.5 mg/mL warmed
(1.91 mM)
Water Insoluble
Ethanol Insoluble

Chemical Information

Molecular Weight 261.23
Formula

C13H11NO5

CAS No. 14698-29-4
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CCN1C=C(C(=O)C2=CC3=C(C=C21)OCO3)C(=O)O

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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