Home DNA Damage/DNA Repair DNA/RNA Synthesis inhibitor Oxolinic acid

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Oxolinic acid DNA/RNA Synthesis inhibitor

Cat.No.S4537

Oxolinic acid (Urinox, NSC 110364) is a quinolone antibiotic, inhibiting the enzyme DNA gyrase and DNA synthesis.It also acts as a dopamine reuptake inhibitor.
Oxolinic acid DNA/RNA Synthesis inhibitor Chemical Structure

Chemical Structure

Molecular Weight: 261.23

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Quality Control

Batch: S453701 0.5MNAOH]6.02 mg/mL]false]DMSO]0.5 mg/mL]false]Water]Insoluble]false Purity: 99.39%
99.39

Solubility

In vitro
Batch:

0.5MNAOH : 6.02 mg/mL

DMSO : 0.5 mg/mL (1.91 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : Insoluble

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In vivo
Batch:

In vivo Formulation Calculator (Clear solution)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight 261.23 Formula

C13H11NO5

 Storage (From the date of receipt)
CAS No. 14698-29-4 Download SDF Storage of Stock Solutions

Synonyms Urinox, NSC 110364 Smiles CCN1C=C(C(=O)C2=CC3=C(C=C21)OCO3)C(=O)O

Mechanism of Action

Targets/IC50/Ki
bacterial DNA gyrase
DNA synthesis
dopamine uptake
4.3 μM
In vitro
Oxolinic acid, a quinolone antibacterial agent, inhibits reversibly the ATP-dependent replicative DNA synthesis in permeable cell systems as well as in cellophane disk lysates. It also inhibits bacterial DNA gyrase but not eukaryotic topoisomerases, reversibly binding gyrase subunit A in gyrase-DNA complexes, blocking supercoiling activity and inhibiting DNA synthesis at 0.5-5 µg/ml.
In vivo
The antimicrobial agent oxolinic acid, injected i.p. in mice, induces a dose dependent increase in locomotor activity. the stimulant locomotor effect of oxolinic acid depends on the blockade of the neuronal dopamine uptake complex.
References
  • [4] https://pubmed.ncbi.nlm.nih.gov/770163/
  • [5] https://pubmed.ncbi.nlm.nih.gov/9293187/
  • [6] https://pubmed.ncbi.nlm.nih.gov/212734/
  • [7] https://pubmed.ncbi.nlm.nih.gov/6274849/

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