Osalmid

Catalog No.S3641

For research use only.

Osalmid suppresses ribonucleotide reductase (RNR) activity in a concentration-dependent manner, with a 50% RR activity-inhibitory concentration (IC50) of 8.23 μM.

Osalmid Chemical Structure

CAS No. 526-18-1

Purity & Quality Control

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Biological Activity

Description Osalmid suppresses ribonucleotide reductase (RNR) activity in a concentration-dependent manner, with a 50% RR activity-inhibitory concentration (IC50) of 8.23 μM.
Targets
RRM2 [1]
(Cell-free assay)
8.23 μM
In vitro

Osalmid is shown to be 10-fold more active in inhibiting RR activity than hydroxyurea, and significantly inhibits HBV DNA and cccDNA synthesis in HepG2.2.15 cells. Osalmid significantly inhibits HBV DNA replication in a time- and dose-dependent manner[1].

In vivo Osalmid significantly reduces RR activity and HBV DNA replication in vivo[1].

Protocol (from reference)

Cell Research:[1]
  • Cell lines: HepG2.2.15 cells
  • Concentrations: 0, 400, 600, 900, 1200 μM
  • Incubation Time: 36 h
  • Method: Cells are treated with various concentrations of osalmid for 36 h.
Animal Research:[1]
  • Animal Models: BALB/c mice
  • Dosages: 400 mg/kg
  • Administration: oral gavage

Solubility (25°C)

In vitro

DMSO 45 mg/mL
(196.3 mM)
Ethanol 45 mg/mL
(196.3 mM)
Water Insoluble

Chemical Information

Molecular Weight 229.23
Formula

C13H11NO3

CAS No. 526-18-1
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1=CC=C(C(=C1)C(=O)NC2=CC=C(C=C2)O)O

In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
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Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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