HQNO

Catalog No.S8970 Synonyms: 2-heptyl-4-hydroxyquinoline-N-oxide

For research use only.

HQNO (2-heptyl-4-hydroxyquinoline-N-oxide) is a natural quinolone secreted by P. aeruginosa. HQNO is a potent inhibitor of the electron transport chain with Kd of 64 nM for the ubiquinol-ferricytochrome c oxidoreductase segment (Complex III) of the respiratory chain. HQNO is also an inhibitor of bacterial and mitochondrialType II NADH : quinone oxidoreductase (NDH-2) with IC50 of 7.3 μM in the presence of 50μM menadione.

HQNO Chemical Structure

CAS No. 341-88-8

Purity & Quality Control

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Biological Activity

Description HQNO (2-heptyl-4-hydroxyquinoline-N-oxide) is a natural quinolone secreted by P. aeruginosa. HQNO is a potent inhibitor of the electron transport chain with Kd of 64 nM for the ubiquinol-ferricytochrome c oxidoreductase segment (Complex III) of the respiratory chain. HQNO is also an inhibitor of bacterial and mitochondrialType II NADH : quinone oxidoreductase (NDH-2) with IC50 of 7.3 μM in the presence of 50μM menadione.
Targets
Complex III [1]
(Cell-free assay)
NDH-2 [2]
(Cell-free assay)
64 nM(Kd) 7.3 μM
In vitro

The aerobic respiratory chain-linked NADH oxidase system of B. cereus KCTC 3674 possesses an HQNO-sensitive NADH:quinone oxidoreductase that lacks an energy coupling site containing FAD as a cofactor (designated NDH-2). The respiratory inhibitor HQNO inhibits the activity of NADH:ubiquinone-1 oxidoreductase and NADH:menadione oxidoreductase by about 40% and 45%, respectively, at a concentration of 20 μM.[3]

Protocol (from reference)

Cell Research:

[3]

  • Cell lines: Bacillus cereus KCTC 3674
  • Concentrations: 10 μM, 20 μM, 30 μM, 40 μM, 50 μM
  • Incubation Time: --
  • Method:

    The assay mixture of NADH:ubiquinone-1 oxidoreductase containes a Triton X-100 extract of B. cereus membranes (10 μg protein), 30 mM KCN, and 50 μM Q-1 in 2 ml of 50 mM MES-KOH (pH 6.0) containing 0.1M NaCl. The activity of NADH: ubiquinone-1 oxidoreductase is measured with various concentrations of HQNO.

  • (Only for Reference)

Solubility (25°C)

In vitro

Ethanol 16 mg/mL
(61.69 mM)
DMSO 9 mg/mL
(34.7 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 259.34
Formula

C16H21NO2

CAS No. 341-88-8
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CCCCCCCC1=CC(=O)C2=CC=CC=C2N1O

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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