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HQNO

Name: HQNO
Brand: Selleck Chemicals
SKU: S8970
Availability: InStock
Rating: 5 (1 reviews)

Cat.No.S8970

HQNO (2-heptyl-4-hydroxyquinoline-N-oxide) is a natural quinolone secreted by P. aeruginosa. This compound is a potent inhibitor of the electron transport chain with Kd of 64 nM for the ubiquinol-ferricytochrome c oxidoreductase segment (Complex III) of the respiratory chain. It is also an inhibitor of bacterial and mitochondrialType II NADH : quinone oxidoreductase (NDH-2) with IC50 of 7.3 μM in the presence of 50μM menadione.

Chemical Structure

Molecular Weight: 259.34

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Quality Control

Batch: S897001 Ethanol]16 mg/mL]false]DMSO]9 mg/mL]false]Water]Insoluble]false Purity: 99.31%

Cited in Nature Medicine for its top-tier quality
COA
NMR
SDS
Datasheet

99.31

Related Targets	Dehydrogenase HSP Transferase P450 (e.g. CYP17) PDE phosphatase PPAR Vitamin Carbohydrate Metabolism Mitochondrial Metabolism
Other Others Inhibitors	BAY 2927088（Sevabertinib） Guanidinoacetic acid BAPTA-AM Blebbistatin Methyl-β-cyclodextrin (MβCD) Carbazochrome sodium sulfonate (AC-17) Ursolic Acid Osthole 4-Methylumbelliferone (4-MU) Dioscin

Solubility

In vitro
Batch:

Ethanol : 16 mg/mL

DMSO : 9 mg/mL (34.7 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.45mg/ml (1.74mM)	Taking the 1 mL working solution as an example, add 50 μL of 9 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Average weight of animals: g

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Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	259.34	Formula	C₁₆H₂₁NO₂	Storage (From the date of receipt)	3 years -20°C powder
CAS No.	341-88-8	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	2-heptyl-4-hydroxyquinoline-N-oxide			Smiles	CCCCCCCC1=CC(=O)C2=CC=CC=C2N1O

Mechanism of Action

Targets/IC₅₀/Ki	Complex III (Cell-free assay) 64 nM(Kd) NDH-2 (Cell-free assay) 7.3 μM
In vitro	The aerobic respiratory chain-linked NADH oxidase system of B. cereus KCTC 3674 possesses an HQNO-sensitive NADH:quinone oxidoreductase that lacks an energy coupling site containing FAD as a cofactor (designated NDH-2). The respiratory inhibitor this compound inhibits the activity of NADH:ubiquinone-1 oxidoreductase and NADH:menadione oxidoreductase by about 40% and 45%, respectively, at a concentration of 20 μM.
References	[1] https://pubmed.ncbi.nlm.nih.gov/831781/ [2] https://pubmed.ncbi.nlm.nih.gov/29621505/ [3] https://pubmed.ncbi.nlm.nih.gov/17244482/

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