Home Cytoskeletal Signaling Kinesin inhibitor H-Cys(Trt)-OH

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H-Cys(Trt)-OH Kinesin inhibitor

Cat.No.S5522

H-Cys(Trt)-OH (L-Cys(Trt)-OH, H-L-Cys(Trt)-OH, S-Tritylcysteine,S-Trityl-L-cysteine) is a specific inhibitor of Eg5 that inhibits Eg5-driven microtubule sliding velocity in a reversible fashion with an IC50 of 500 nM.
H-Cys(Trt)-OH Kinesin inhibitor Chemical Structure

Chemical Structure

Molecular Weight: 363.47

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Quality Control

Batch: S552201 DMSO]5 mg/mL]false]]]false]]]false Purity: 98%
98

Solubility

In vitro
Batch:

DMSO : 5 mg/mL (13.75 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

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In vivo
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Chemical Information, Storage & Stability

Molecular Weight 363.47 Formula

C22H21NO2S

 Storage (From the date of receipt) 3 years -20°C powder
CAS No. 2799-07-7 -- Storage of Stock Solutions

Synonyms L-Cys(Trt)-OH, H-L-Cys(Trt)-OH, S-Tritylcysteine, S-Trityl-L-cysteine Smiles C1=CC=C(C=C1)C(C2=CC=CC=C2)(C3=CC=CC=C3)SCC(C(=O)O)N

Mechanism of Action

Targets/IC50/Ki
Eg5
In vitro

In cell-based assays, H-Cys(Trt)-OH (S-trityl-l-cysteine) does not prevent cell cycle progression at the S or G2 phases but inhibits both separation of the duplicated centrosomes and bipolar spindle formation, thereby blocking cells specifically in the M phase of the cell cycle with monoastral spindles. Following removal of S-trityl-l-cysteine, mitotically arrested cells exit mitosis normally. In vitro, S-trityl-l-cysteine targets the catalytic domain of Eg5 and inhibits Eg5 basal and microtubule-activated ATPase activity as well as mant-ADP release. It is a tight binding inhibitor and inhibits Eg5-driven microtubule sliding velocity in a reversible fashion with an IC50 of 500 nM.
References

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