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H-Cys(Trt)-OH Kinesin inhibitor

Name: H-Cys(Trt)-OH
Brand: Selleck Chemicals
SKU: S5522
Availability: InStock
Rating: 5 (2 reviews)

Cat.No.S5522

H-Cys(Trt)-OH (L-Cys(Trt)-OH, H-L-Cys(Trt)-OH, S-Tritylcysteine,S-Trityl-L-cysteine) is a specific inhibitor of Eg5 that inhibits Eg5-driven microtubule sliding velocity in a reversible fashion with an IC50 of 500 nM.

Chemical Structure

Molecular Weight: 363.47

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Quality Control

Batch: S552201 DMSO]5 mg/mL]false]]]false]]]false Purity: 98%

Cited in Nature Medicine for its top-tier quality
COA
SDS/MSDS
Datasheet

Related Targets	Akt Wnt/beta-catenin PKC HSP ROCK Microtubule Associated Integrin Bcr-Abl Actin FAK
Other Kinesin Inhibitors	GSK-923295 Ispinesib (SB-715992) SB743921 HCl Monastrol ARQ 621 GW406108X VLS-1488(KIF18A-IN-6 ) SB-743921 K 858 BTB-1

Solubility

In vitro
Batch:

DMSO : 5 mg/mL (13.75 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.25mg/ml (0.69mM)	Taking the 1 mL working solution as an example, add 50 μL of 5 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.036mg/ml (0.10mM)	Taking the 1 mL working solution as an example, add 50 μL of 0.72 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

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Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	363.47	Formula	C₂₂H₂₁NO₂S	Storage (From the date of receipt)	3 years -20°C powder
CAS No.	2799-07-7	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	L-Cys(Trt)-OH, H-L-Cys(Trt)-OH, S-Tritylcysteine, S-Trityl-L-cysteine			SMILES	C1=CC=C(C=C1)C(C2=CC=CC=C2)(C3=CC=CC=C3)SCC(C(=O)O)N

Read more about storage stability stock solution CAS number SMILES

Mechanism of Action

Targets/IC₅₀/Ki	Eg5
In vitro	In cell-based assays, H-Cys(Trt)-OH (S-trityl-l-cysteine) does not prevent cell cycle progression at the S or G2 phases but inhibits both separation of the duplicated centrosomes and bipolar spindle formation, thereby blocking cells specifically in the M phase of the cell cycle with monoastral spindles. Following removal of S-trityl-l-cysteine, mitotically arrested cells exit mitosis normally. In vitro, S-trityl-l-cysteine targets the catalytic domain of Eg5 and inhibits Eg5 basal and microtubule-activated ATPase activity as well as mant-ADP release. It is a tight binding inhibitor and inhibits Eg5-driven microtubule sliding velocity in a reversible fashion with an IC50 of 500 nM.
References	[1] https://pubmed.ncbi.nlm.nih.gov/16507573/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2026-08-18)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT06427798	RECRUITING	Somatostatin Receptor Positive; Gastrointestinal Neuroendocrine Tumors; Pheochromocytoma; Paragangliomas	National Cancer Institute (NCI)	2025-02-07	PHASE1; PHASE2
NCT06256705	RECRUITING	Gastroenteropancreatic Neuroendocrine Tumor; Radiotherapy	Assistance Publique - Hôpitaux de Paris	2024-03-25
NCT05839730	ACTIVE_NOT_RECRUITING	Heart Failure With Preserved Ejection Fraction; Concentric Hypertrophy	Medtronic Cardiac Rhythm and Heart Failure	2024-09-11
NCT05351320	UNKNOWN	Non-small Cell Lung Cancer (NSCLC)	Jinming Yu	2022-04-30	PHASE2
NCT05386888	COMPLETED	NSCLC, Stage III	Genfleet Therapeutics (Shanghai) Inc.	2022-09-09	PHASE2
NCT05684874	UNKNOWN	Soft Tissue Sarcoma; Adult Soft Tissue Sarcoma; Resectable Soft Tissue Sarcoma	Centre Leon Berard	2023-04-21

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