H-Cys(Trt)-OH

Catalog No.S5522 Synonyms: L-Cys(Trt)-OH, H-L-Cys(Trt)-OH, S-Tritylcysteine

H-Cys(Trt)-OH Chemical Structure

Molecular Weight(MW): 363.47

H-Cys(Trt)-OH is a specific inhibitor of Eg5 that inhibits Eg5-driven microtubule sliding velocity in a reversible fashion with an IC50 of 500 nM.

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Biological Activity

Description H-Cys(Trt)-OH is a specific inhibitor of Eg5 that inhibits Eg5-driven microtubule sliding velocity in a reversible fashion with an IC50 of 500 nM.
Targets
Eg5 [1]
()
In vitro

In cell-based assays, H-Cys(Trt)-OH (S-trityl-l-cysteine) does not prevent cell cycle progression at the S or G2 phases but inhibits both separation of the duplicated centrosomes and bipolar spindle formation, thereby blocking cells specifically in the M phase of the cell cycle with monoastral spindles. Following removal of S-trityl-l-cysteine, mitotically arrested cells exit mitosis normally. In vitro, S-trityl-l-cysteine targets the catalytic domain of Eg5 and inhibits Eg5 basal and microtubule-activated ATPase activity as well as mant-ADP release. It is a tight binding inhibitor and inhibits Eg5-driven microtubule sliding velocity in a reversible fashion with an IC50 of 500 nM[1].

Protocol

Cell Research:

[1]

+ Expand
  • Cell lines: HeLa cells and U2OS cells
  • Concentrations: 5 μM
  • Incubation Time: 0, 24, 48, 72 h
  • Method:

    HeLa cells and U2OS cells were analyzed by FACScan analysis following incubation with STLC (5 μM) at the indicated times.


    (Only for Reference)

Solubility (25°C)

In vitro DMSO 5 mg/mL (13.75 mM)

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 363.47
Formula

C22H21NO2S

CAS No. 2799-07-7
Storage powder
in solvent
Synonyms L-Cys(Trt)-OH, H-L-Cys(Trt)-OH, S-Tritylcysteine

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID