Name: FPS-ZM1
Brand: Selleck Chemicals
SKU: S8185
Availability: InStock
Rating: 5 (31 reviews)

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Quality Control

Batch: Purity: 99.91%

99.91

Related Targets	Adrenergic Receptor AChR 5-HT Receptor COX Calcium Channel Histamine Receptor Dopamine Receptor GABA Receptor TRP Channel Cholinesterase (ChE)
Other Beta Amyloid Products	EUK 134 (-)-epigallocatechin Ginsenoside Rg1 Ginsenoside Re Neuropeptide Y Hoechst 34580 Anti-Amyloid Beta (CSIRO anti-amyloid Beta scFv) β-Amyloid (25-35) Anti-Amyloid Beta (U.Illinois scFv59) Donanemab (Anti-Amyloid Beta)

Cell Culture, Treatment & Working Concentration

Cell Lines	Assay Type	Activity Description	PMID
CHO	Function assay	Displacement of [I125]amyloid beta (1 to 40) from human RAGE domain V expressed in CHO cells, Ki=0.025μM	28482155
CHO	Function assay	Inhibition of HMGB1 binding to human RAGE domain V expressed in CHO cells, Ki=0.148μM	28482155
CHO	Function assay	Inhibition of S110B binding to human RAGE domain V expressed in CHO cells, Ki=0.23μM	28482155
Click to View More Cell Line Experimental Data

Solubility

In vitro
Batch:

DMSO : 66 mg/mL (201.31 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 66 mg/mL

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	3.250mg/ml (9.91mM)	Taking the 1 mL working solution as an example, add 50 μL of 65 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	3.250mg/ml (9.91mM)	Taking the 1 mL working solution as an example, add 50 μL of 65 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

%

% Tween 80

% ddH₂O

% DMSO

+

%

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	327.85	Formula	C₂₀H₂₂ClNO	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	945714-67-0	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	N/A			Smiles	C1CCC(CC1)N(CC2=CC=CC=C2)C(=O)C3=CC=C(C=C3)Cl

Mechanism of Action

Targets/IC₅₀/Ki	RAGE
In vitro	FPS-ZM1 blocks Aβ binding to the V domain of RAGE and inhibited Aβ40- and Aβ42-induced cellular stress in RAGE-expressing cells in vitro. It blocks binding of other ligands to RAGE as well, such as S100B, AGE, and HMGB1, which have been suggested to contribute to RAGE-mediated long-term tissue damage in models of diabetes, immune/inflammatory disorders, and AD.
In vivo	FPS-ZM1 is nontoxic to mice and readily crossed the blood-brain barrier (BBB). It effectively controls progression of an Aβ-mediated brain disorder and the related neurovascular and cognitive dysfunction in 17-month-old APPsw/0 mice with fully developed Aβ and amyloid pathology by blocking RAGE actions at the BBB and in brain. Also, this compound blocks RAGE-dependent BACE1 expression and activity in brain of 17-month-old APPsw/0 mice.
References	[1] https://pubmed.ncbi.nlm.nih.gov/22406537/

Applications

Methods	Biomarkers	Images	PMID
Immunofluorescence	AGE-2 / AGEE-3 cathepsin B Caspase-1		29430285

Tech Support

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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FPS-ZM1 Beta Amyloid inhibitor

Chemical Structure

Molecular Weight: 327.85