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Forchlorfenuron (CPPU)

Cat.No.S5306

Forchlorfenuron (CPPU) is a synthetic cytokinin that inhibits septins and exhibits anti-parasitic, anticancer, and anti-angiogenic activities.
Forchlorfenuron (CPPU) Chemical Structure

Chemical Structure

Molecular Weight: 247.68

Jump to Mechanism of Action Solubility Chemical Information, Storage & Stability

Quality Control

Batch: S530601 DMSO]49 mg/mL]false]]]false]]]false Purity: 99.92%
99.92

Chemical Information, Storage & Stability

Molecular Weight 247.68 Formula

C12H10ClN3O

 Storage (From the date of receipt)
CAS No. 68157-60-8 -- Storage of Stock Solutions

Synonyms KT-30 Smiles C1=CC=C(C=C1)NC(=O)NC2=CC(=NC=C2)Cl

Solubility

In vitro
Batch:

DMSO : 49 mg/mL (197.83 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Mechanism of Action

Targets/IC50/Ki
septins [1]
In vitro
Forchlorfenuron (FCF) is a plant cytokinin shown to disrupt septin localization in budding yeast. FCF alters septin assembly in vitro without affecting either actin or tubulin polymerization. In live mammalian cells, FCF dampens septin dynamics and induces the assembly of abnormally large septin structures. FCF has a low level of cytotoxicity, and these effects are reversed upon FCF washout. Significantly, FCF treatment induces mitotic and cell migration defects that phenocopy the effects of septin depletion by small interfering RNA. FCF targets the filamentous assembly of divergent septin isoforms (e.g. Cdc3/10/11/12 (42), SEPT2, SEPT7) reversibly and across species[1].
In vivo
Forchlorfenuron (FCF) has low oral toxicity in rats with an LD50 of 4940 mg/kg bw in male rats and 4899 mg/kg bw in females[2].
References

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