Forchlorfenuron (CPPU)

Catalog No.S5306 Batch:S530601

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Technical Data

Formula

C12H10ClN3O
Molecular Weight 247.68 CAS No. 68157-60-8
Solubility (25°C)* In vitro DMSO 49 mg/mL (197.83 mM)
 
 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Forchlorfenuron (CPPU) is a synthetic cytokinin that inhibits septins and exhibits anti-parasitic, anticancer, and anti-angiogenic activities.
Targets
septins [1]
In vitro Forchlorfenuron (FCF) is a plant cytokinin shown to disrupt septin localization in budding yeast. FCF alters septin assembly in vitro without affecting either actin or tubulin polymerization. In live mammalian cells, FCF dampens septin dynamics and induces the assembly of abnormally large septin structures. FCF has a low level of cytotoxicity, and these effects are reversed upon FCF washout. Significantly, FCF treatment induces mitotic and cell migration defects that phenocopy the effects of septin depletion by small interfering RNA. FCF targets the filamentous assembly of divergent septin isoforms (e.g. Cdc3/10/11/12 (42), SEPT2, SEPT7) reversibly and across species[1].
In vivo Forchlorfenuron (FCF) has low oral toxicity in rats with an LD50 of 4940 mg/kg bw in male rats and 4899 mg/kg bw in females[2].

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    HeLa or MDCK cells

  • Concentrations

    6.25-500 μM

  • Incubation Time

    4 h

  • Method

    HeLa or MDCK cells are plated overnight on collagen-coated 35-mm tissue culture dishes and then treated with DMSO or FCF for 4 h. Cells are trypsinized, resuspended in DMEM supplemented with 10% FBS, and mixed in a 1:1 ratio with trypan blue at room temperature for 1 min. Blue and non-blue cells are counted using a hemocytometer.

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.