Name: Doxorubicin
Brand: Selleck Chemicals
SKU: E2516
Rating: 5 (74 reviews)

Adriamycin (Doxorubicin, Hydroxydaunorubicin), a cytotoxic anthracycline antibiotic, is an anti-cancer chemotherapy agent, inhibits topoisomerase II with an IC50 of 2.67 μM, thus stopping DNA replication, and induces apoptosis, also inhibits human DNA topoisomerase I with an IC50 of 0.8 μM.

Doxorubicin Chemical Structure

CAS: 23214-92-8

Selleck's Doxorubicin has been cited by 74 publications

Purity & Quality Control

Batch: Purity: 99.71%

99.71

Products often used together with Doxorubicin

Paclitaxel

Doxorubicin and Paclitaxel use exhibit more significant tumor regression in vivo.

Jin C, et al. J Cancer Res Clin Oncol. 2010 Feb;136(2):267-74.

MK-2206 2HCl

Doxorubicin and MK-2206 2HCl exert a profound inhibitory effect on MMP-2 and MMP-9 expression in H929 cells.

Yang YL, et al. Zhongguo shi yan xue ye xue za zhi 30.2 (2022): 487-492

Cisplatin

Doxorubicin and Cisplatin are two commonly co-administered chemotherapy drugs used to treat a wide range of cancers.

Lopes F, et al. Reproduction. 2020 Feb; 159(2): 193–204.

Bortezomib

Doxorubicin and Bortezomib combination use enhances apoptotic activity and suppresses anti-apoptotic pathways in various tumour model systems.

Plosker GL, Drugs 68 (2008): 2535-2551.

Cyclophosphamide

Doxorubicin and Cyclophosphamide therapy suppress tumor growth in both tumor-specific and T-cell-dependent manners.

Tongu M, et al. Cancer Immunol Immunother. 2010 May;59(5):769-77.

Doxorubicin Related Products

Related Targets	Topo I Topo II Topo IV	Click to Expand
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Related Compound Libraries	FDA-approved Drug Library Natural Product Library Apoptosis Compound Library DNA Damage/DNA Repair compound Library Cell Cycle compound library	Click to Expand

Signaling Pathway

Topoisomerase Signaling Pathway

Choose Selective Topoisomerase Inhibitors

Biological Activity

Description

Adriamycin (Doxorubicin, Hydroxydaunorubicin), a cytotoxic anthracycline antibiotic, is an anti-cancer chemotherapy agent, inhibits topoisomerase II with an IC50 of 2.67 μM, thus stopping DNA replication, and induces apoptosis, also inhibits human DNA topoisomerase I with an IC50 of 0.8 μM.

Targets

topoisomerase I ^[3] (in assay of DNA Topo I activity)	topoisomerase II ^[1] (in decatenation assay for topoisomerase II activity)
0.8 μM	2.67 μM

References

Chemical Information & Solubility

Molecular Weight	543.52	Formula	C₂₇H₂₉NO₁₁
CAS No.	23214-92-8	SDF	--
Smiles	COC1=C2C(=O)C3=C(C(=C4CC(O)(CC(OC5CC(N)C(O)C(C)O5)C4=C3O)C(=O)CO)O)C(=O)C2=CC=C1
Storage (From the date of receipt)	3 years -20°C powder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years -20°C powder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 100 mg/mL ( (183.98 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 100 mg/mL

Ethanol : Insoluble

Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.

In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Dilution Calculator Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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