research use only

Clofazimine Dyes inhibitor

Name: Clofazimine
Brand: Selleck Chemicals
SKU: S4107
Availability: InStock
Rating: 5 (6 reviews)

Cat.No.S4107

Clofazimine (NSC-141046) is a rhimophenazine dye, originally developed for the treatment of tuberculosis, it has both antimicrobial and antiinflammatory activity, postulated mechanisms of action of this compound include intercalation of this chemical with bacterial DNA and increasing levels of cellular phospholipase A2.

Chemical Structure

Molecular Weight: 473.4

Jump to

Quality Control

Batch: Purity: 99.53%

99.53

Related Targets	Dehydrogenase HSP Transferase P450 (e.g. CYP17) PDE phosphatase PPAR Vitamin Carbohydrate Metabolism Mitochondrial Metabolism
Other Dyes Inhibitors	Fluorofurimazine (FFz) CFSE (CFDA-SE) Propidium Iodide DFO Thiazolyl Blue Hoechst 33342 D-Luciferin Crystal Violet D-Luciferin sodium salt Dihydroethidium

Solubility

In vitro
Batch:

DMSO : 5 mg/mL (10.56 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : Insoluble

Ethanol : Insoluble

DMSO : 5 mg/mL (10.56 mM) Warmed with 60°C water bath; Ultrasonicated;
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.15mg/ml (0.32mM)	Taking the 1 mL working solution as an example, add 50 μL of 3 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.15mg/ml (0.32mM)	Taking the 1 mL working solution as an example, add 50 μL of 3 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	473.4	Formula	C₂₇H₂₂Cl₂N₄	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	2030-63-9	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	NSC-141046			SMILES	CC(C)N=C1C=C2C(=NC3=CC=CC=C3N2C4=CC=C(C=C4)Cl)C=C1NC5=CC=C(C=C5)Cl

Read more about storage stability stock solution CAS number SMILES

Mechanism of Action

Features	Intracellular activities of Clofazimine are superior to that of B4154.
Targets/IC₅₀/Ki	PLA2
In vitro	Clofazimine stimulates oxygen consumption and superoxide generation by neutrophils, this compound also causes phospholipase A2 activation in neutrophils, resulting in increased release of lysophosphatidylcholine and arachidonic acid from neutrophil membranes. It inhibits mitogen-induced stimulation of peripheral blood mononuclear cells. This chemical stabilizes lysosomal membranes in macrophages and inhibits Mycobacterium leprae metabolism in mouse peritoneal macrophages. It (5 mg/mL) causes dose-related enhancement of the activity of phospholipase A2 in S. aureus, according to an increase in the release of 3H-radiolabeled arachidonate and lysophosphatidylethanolamine ([3H]LPE) from bacterial-membrane phospholipids. This compound inhibits 90% of twenty M. tuberculosis strains with MICs <1.0 μg/mL, to be noted, it inhibits M. tuberculosis strain 2227 with MICs of 0.06 μg/mL. It (1 μg/mL) dose-dependently inhibits activity of J774A.1 macrophages.
In vivo	Clofazimine (20 mg/kg) prevents mortality and causes a significant reduction in the numbers of CFU in the lungs and spleens of C57BL/6 mice infected M.tuberculosis H37Rv. Liposomal Clofazimine (L-CLF) (50 mg/kg) dose-dependently reduces CFU 2 to 3 log units in the spleen, liver, and lungs of acutely infected mice with Mycobacterium tuberculosis Erdman. This compound (500 μg, bid) results in highest Clofazimine concentrations in spleens and livers of mice whereas Clofazimine concentrations in the lungs are significantly lower. This chemical (20 mg/kg) is effective in reducing bacterial loads in the liver, spleen and lungs of C57BL/6 mice experimentally infected with M. avium strain TMC 724.
References	[1] https://pubmed.ncbi.nlm.nih.gov/7829710/ [2] https://pubmed.ncbi.nlm.nih.gov/1482140/ [3] https://pubmed.ncbi.nlm.nih.gov/8851584/ [4] https://pubmed.ncbi.nlm.nih.gov/10390215/ [5] https://pubmed.ncbi.nlm.nih.gov/10629016/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2026-03-20)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT07485010	NOT_YET_RECRUITING	Mycobacterium Abscessus Pulmonary Disease; Mycobacterium Abscessus Infection; Non-Tuberculous Mycobacterial (NTM) Infections; Non-Tuberculous Mycobacteria Pulmonary Disease	The University of Queensland	2027-04	PHASE2
NCT04310930	RECRUITING	Pulmonary Disease Due to Mycobacteria (Diagnosis)	The University of Queensland	2020-03-02	PHASE2; PHASE3
NCT07228702	ENROLLING_BY_INVITATION	Non-Tuberculous Mycobacterial (NTM) Pneumonia; Mycobacterium Abscessus Infection	Vancouver Coastal Health	2026-01-11	PHASE1
NCT07198685	NOT_YET_RECRUITING	Treat to Target; Treatment Duration	Wuhan Pulmonary Hospital	2025-09-30
NCT07126639	NOT_YET_RECRUITING	Efficacy and Safety	Wuhan Pulmonary Hospital	2025-09-01
NCT03828201	ACTIVE_NOT_RECRUITING	Tuberculosis, Multidrug-Resistant	Boston University	2022-06-07	PHASE2

Tech Support

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):