Home Cytoskeletal Signaling Actin inhibitor CK-666 For research use only.

CK-666

CK-666 is an inhibitor of the actin-related protein 2/3 (Arp2/3) complex with IC50 of 17 μM and 5 μM for BtArp2/3 complex and SpArp2/3 complex, respectively.

CK-666 Chemical Structure

CK-666 Chemical Structure

CAS: 442633-00-3

Purity & Quality Control

Batch: S769001 DMSO] 59 mg/mL] false] Ethanol] 59 mg/mL] false] Water] Insoluble] false Purity: 99.84%
99.84

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Biological Activity

Description CK-666 is an inhibitor of the actin-related protein 2/3 (Arp2/3) complex with IC50 of 17 μM and 5 μM for BtArp2/3 complex and SpArp2/3 complex, respectively.
Targets
SpArp2/3 [1]
(Cell-free assay)		 BtArp2/3 [1]
(Cell-free assay)
5 μM 17 μM
In vitro
In vitro

In SKOV3 cells, CK-666 reduces actin polymerization around intracellular Listeria to background levels. Actin filament halos and comet tails reformed when CK-666 is removed after one hour of treatment.[1]
Cell Research Cell lines SKOV3 cells
Concentrations 40 μM
Incubation Time 60 min
Method

Infected SKOV3 cells are treated with 40 μM CK-666 for 60 min followed by a 60 min washout. The pairs of fluorescence micrographs show anti-Listeria fluorescence (top) and Alexa 488 phalloidin fluorescence.
In Vivo
In vivo

CK-666 suppresses the enhanced memory phenotype of msi-1(lf) by inhibiting the Arp2/3 complex activity. In addition, inhibition of Arp2/3, involved in actin filament branching and neuronal morphogenesis, in the lateral amygdala (LA) with the specific inhibitor CK-666 during fear conditioning impaired long-term fear memory in rats.[2]
Animal Research Animal Models Rats
Dosages 100 µM, 0.5 µl
Administration i.c.v

Chemical Information & Solubility

Molecular Weight 296.34 Formula

C18H17FN2O
CAS No. 442633-00-3 SDF --
Smiles CC1=C(CCNC(=O)C2=CC=CC=C2F)C3=C([NH]1)C=CC=C3
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 59 mg/mL ( (199.09 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 59 mg/mL

Water : Insoluble


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In vivo
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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