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CK-666 Actin inhibitor

Name: CK-666
Brand: Selleck Chemicals
SKU: S7690
Availability: InStock
Rating: 5 (2 reviews)

Cat.No.S7690

CK-666 is an inhibitor of the actin-related protein 2/3 (Arp2/3) complex with IC50 of 17 μM and 5 μM for BtArp2/3 complex and SpArp2/3 complex, respectively.

Chemical Structure

Molecular Weight: 296.34

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Quality Control

Batch: Purity: 99.84%

99.84

Related Targets	Akt Wnt/beta-catenin HSP PKC ROCK Microtubule Associated Integrin Bcr-Abl FAK Kinesin
Other Actin Products	Cytochalasin D CK-636 Benproperine phosphate Arp3 C-terminal Antibody [F20N23] SMIFH2 δ Sarcoglycan Antibody [K7B23] ACTL6A/BAF53A Antibody [F6F1] Cytokeratin 5 Antibody [G20N20] N-WASP Antibody [K15N2] Neurofilament-M Antibody [H7L9]

Solubility

In vitro
Batch:

DMSO : 59 mg/mL (199.09 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 59 mg/mL

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	15%DMSO 1 40%PEG300 2 5%TWEEN80 3 40%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	7.500mg/ml (25.31mM)	Taking the 1 mL working solution as an example, add 150 μL of 50 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 400 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	2.950mg/ml (9.95mM)	Taking the 1 mL working solution as an example, add 50 μL of 59 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.490mg/ml (1.65mM)	Taking the 1 mL working solution as an example, add 50 μL of 9.8 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	296.34	Formula	C₁₈H₁₇FN₂O	Storage (From the date of receipt)	3 years -20°C powder
CAS No.	442633-00-3	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	N/A			Smiles	CC1=C(CCNC(=O)C2=CC=CC=C2F)C3=C([NH]1)C=CC=C3

Mechanism of Action

Targets/IC₅₀/Ki	SpArp2/3 (Cell-free assay) 5 μM BtArp2/3 (Cell-free assay) 17 μM
In vitro	In SKOV3 cells, CK-666 reduces actin polymerization around intracellular Listeria to background levels. Actin filament halos and comet tails reformed when this compound is removed after one hour of treatment.
In vivo	CK-666 suppresses the enhanced memory phenotype of msi-1(lf) by inhibiting the Arp2/3 complex activity. In addition, inhibition of Arp2/3, involved in actin filament branching and neuronal morphogenesis, in the lateral amygdala (LA) with this compound during fear conditioning impaired long-term fear memory in rats.
References	[1] https://pubmed.ncbi.nlm.nih.gov/19648907/ [2] https://pubmed.ncbi.nlm.nih.gov/27957528/

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