Home Cytoskeletal Signaling Actin inhibitor SMIFH2

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SMIFH2 Actin inhibitor

Cat.No.E1042

SMIFH2 is a specific inhibitor of formin that inhibits formin-driven actin polymerization in vitro with IC50s ranging from 5 to 15 μM for different formins.

SMIFH2 Actin inhibitor Chemical Structure

Chemical Structure

Molecular Weight: 377.21

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Quality Control

Batch: E104201 DMSO]75 mg/mL]false]Water]Insoluble]false]Ethanol]Insoluble]false Purity: 99.19%
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99.19

Solubility

In vitro
Batch:

DMSO : 75 mg/mL (198.82 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : Insoluble

Ethanol : Insoluble

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In vivo
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Chemical Information, Storage & Stability

Molecular Weight 377.21 Formula

C15H9BrN2O3S

 Storage (From the date of receipt) 3 years -20°C powder
CAS No. 340316-62-3 -- Storage of Stock Solutions

Synonyms N/A Smiles BrC1=CC=CC(=C1)N2C(=S)NC(=O)C(=C\C3=CC=CO3)/C2=O

Mechanism of Action

Targets/IC50/Ki
formin
In vitro

SMIFH2 inhibits stress fiber contractility in living and permeabilized REF52 cells. This compound inhibits centripetal movement of myosin 2 filaments in living and permeabilized HFF cells. It inhibits activity of myosin ATPase and the ability to translocate actin filaments in the gliding actin.
In vivo

SMIFH2 induces tail curvature and peripheral cell death, craniofacial malformations, pericardial edema in developing zebrafish embryos. This compound impairs vascular flow and impacts heart rates in developing zebrafish embryos.
References

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