Home Cytoskeletal Signaling Actin inhibitor SMIFH2 For research use only.

SMIFH2

SMIFH2 is a specific inhibitor of formin that inhibits formin-driven actin polymerization in vitro with IC50s ranging from 5 to 15 μM for different formins.

SMIFH2 Chemical Structure

SMIFH2 Chemical Structure

CAS: 340316-62-3

Purity & Quality Control

Batch: E104201 DMSO] 75 mg/mL] false] Water] Insoluble] false] Ethanol] Insoluble] false Purity: 99.19%
99.19

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Biological Activity

Description

SMIFH2 is a specific inhibitor of formin that inhibits formin-driven actin polymerization in vitro with IC50s ranging from 5 to 15 μM for different formins.
Targets
formin [1]
In vitro
In vitro

SMIFH2 inhibits stress fiber contractility in living and permeabilized REF52 cells. SMIFH2 inhibits centripetal movement of myosin 2 filaments in living and permeabilized HFF cells. SMIFH2 inhibits activity of myosin ATPase and the ability to translocate actin filaments in the gliding actin.[1]
Cell Research Cell lines REF52 cells, HFF cells
Concentrations 30 μM, 100 μM
Incubation Time 10 min, 45 min
Method

Cells are seeded at density of 5×104 cells/ml on the hydrophobic uncoated μ-dish 35 mm with fibronectin micro-patterns and incubated 3-8 h prior to the experiment. Cells are permeabilized with extraction buffer A supplemented with 0.1% Triton X-100 and 4% PEG MW35000 for 10 min at room temperature, followed by three washes with extraction buffer A. Cytoskeleton contractility assay was carried out at 37°C using buffer A supplemented with 2 mM ATP with or without SMIFH2.
In Vivo
In vivo

SMIFH2 induces tail curvature and peripheral cell death, craniofacial malformations, pericardial edema in developing zebrafish embryos. SMIFH2 impairs vascular flow and impacts heart rates in developing zebrafish embryos.[2]

Chemical Information & Solubility

Molecular Weight 377.21 Formula

C15H9BrN2O3S
CAS No. 340316-62-3 SDF --
Smiles BrC1=CC=CC(=C1)N2C(=S)NC(=O)C(=C\C3=CC=CO3)/C2=O
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 75 mg/mL ( (198.82 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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