CK-636

Catalog No.S7497 Synonyms: CK-0944636

For research use only.

CK-636 (CK-0944636) is an Arp2/3 complex inhibitor with IC50 of 4 μM, 24 μM and 32 μM for inhibition of actin polymerization induced by human, fission yeast and bovine Arp2/3 complex, respectively.

CK-636 Chemical Structure

CAS No. 442632-72-6

Selleck's CK-636 has been cited by 4 Publications

Purity & Quality Control

Choose Selective Actin Inhibitors

Biological Activity

Description CK-636 (CK-0944636) is an Arp2/3 complex inhibitor with IC50 of 4 μM, 24 μM and 32 μM for inhibition of actin polymerization induced by human, fission yeast and bovine Arp2/3 complex, respectively.
Targets
Human Arp2/3 complex [1] Fission yeast Arp2/3 complex [1] Bovine Arp2/3 complex [1]
4 μM 24 μM 32 μM
In vitro

CK-636 inhibits actin polymerization mediated by Arp2/3 complex in live cells, and reduces the formation of actin filament comet tails by Listeria in infected SKOV3 cells. [1] CK-636 inhibits lamellipodia formation at the leading edges of migrating T cells, and causes reduced velocity. In addition, a substantial fraction of CK-636-treated T cells on zigzag patterns with an acute turning angle are trapped near the interfaces formed by the turning points of zigzag patterns. [2]

Protocol (from reference)

Solubility (25°C)

In vitro

DMSO 57 mg/mL
(200.43 mM)
Water Insoluble
Ethanol ''50 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 284.38
Formula

C16H16N2OS

CAS No. 442632-72-6
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC1=C(C2=CC=CC=C2N1)CCNC(=O)C3=CC=CS3

In vivo Formulation Calculator (Clear solution)

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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