CK-666

Catalog No.S7690 Batch:S769001

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Technical Data

Formula

C18H17FN2O
Molecular Weight 296.34 CAS No. 442633-00-3
Solubility (25°C)* In vitro DMSO 59 mg/mL (199.09 mM)
Ethanol 59 mg/mL (199.09 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description CK-666 is an inhibitor of the actin-related protein 2/3 (Arp2/3) complex with IC50 of 17 μM and 5 μM for BtArp2/3 complex and SpArp2/3 complex, respectively.
Targets
SpArp2/3 [1]
(Cell-free assay)		 BtArp2/3 [1]
(Cell-free assay)
5 μM 17 μM
In vitro

In SKOV3 cells, CK-666 reduces actin polymerization around intracellular Listeria to background levels. Actin filament halos and comet tails reformed when CK-666 is removed after one hour of treatment.[1]
In vivo

CK-666 suppresses the enhanced memory phenotype of msi-1(lf) by inhibiting the Arp2/3 complex activity. In addition, inhibition of Arp2/3, involved in actin filament branching and neuronal morphogenesis, in the lateral amygdala (LA) with the specific inhibitor CK-666 during fear conditioning impaired long-term fear memory in rats.[2]

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    SKOV3 cells

  • Concentrations

    40 μM

  • Incubation Time

    60 min

  • Method

    Infected SKOV3 cells are treated with 40 μM CK-666 for 60 min followed by a 60 min washout. The pairs of fluorescence micrographs show anti-Listeria fluorescence (top) and Alexa 488 phalloidin fluorescence.
Animal Study:

[2]
  • Animal Models

    Rats

  • Dosages

    100 µM, 0.5 µl

  • Administration

    i.c.v

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.