Name: Chloroquine
Brand: Selleck Chemicals
SKU: S6999
Rating: 5 (138 reviews)

Chloroquine is an antimalarial drug and autophagy/lysosome inhibitor. Chloroquine also suppresses Toll-like receptor-9 (TLR9) protein expression. Chloroquine is highly effective agianst SARS-CoV-2 (COVID-19) infection with EC50 of 1.13 μM in Vero E6 cells. Chloroquine has anti-HIV-1 activity.

Chloroquine Chemical Structure

CAS: 54-05-7

Selleck's Chloroquine has been cited by 138 publications

Purity & Quality Control

Batch: Purity: 99.84%

99.84

Chloroquine Related Products

Signaling Pathway

Autophagy Signaling Pathway

Choose Selective Autophagy Inhibitors

Biological Activity

Description

Chloroquine is an antimalarial drug and autophagy/lysosome inhibitor. Chloroquine also suppresses Toll-like receptor-9 (TLR9) protein expression. Chloroquine is highly effective agianst SARS-CoV-2 (COVID-19) infection with EC50 of 1.13 μM in Vero E6 cells. Chloroquine has anti-HIV-1 activity.

Targets

Autophagy ^[1]	lysosome ^[1]	TLR9 ^[2]	HIV-1 ^[4]	SARS-CoV-2 ^[3] (Vero E6 cells based assay)
				1.13 μM(EC50)

In vitro
In vitro	Chloroquine is a widely used autophagy inhibitor.^[5]
Cell Research	Cell lines	HL‐60 cells
	Concentrations	10 µM
	Incubation Time	48 h
	Method	One day before treatment, AML cells were seeded with a density of 2 × 10⁵ cells/mL. Fresh medium containing different concentrations of Ara‐C was added into the culture medium. Cells were further cultured for 24 or 48 h based on experimental purposes. For inhibition of autophagy, HL‐60 cells were treated with 200 nM bafilomycin A1 or 10 µM chloroquine. Overexpression and knockdown were performed by transfection. (Data sourced from selleck products)

In Vivo
In vivo	The inhibition of autophagy by chloroquine potentiates the therapeutic effect of PI3K/AKT inhibitors in vitro and in vivo TNBC models, synergizing with taxane-based chemotherapy.^[6]
Animal Research	Animal Models	Female NOD/SCID athymic mice injected with MDAMB231 cells
	Dosages	30 mg/kg
	Administration	o.g. (Data sourced from selleck products)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-01-11)
NCT06117722	Not yet recruiting	Chronic Heart Failure	Elpen Pharmaceutical Co. Inc.	January 1 2024	--
NCT05972551	Not yet recruiting	Osteogenesis Imperfecta	Amgen	January 5 2024	Phase 3
NCT03994874	Not yet recruiting	Congestive Heart Failure	Iperboreal Pharma Srl	November 2023	Phase 1\|Phase 2
NCT06007742	Not yet recruiting	ADHD	The Hong Kong Polytechnic University	October 15 2023	Not Applicable

References

+ Expand

Chemical Information & Solubility

Molecular Weight	319.87	Formula	C₁₈H₂₆ClN₃
CAS No.	54-05-7	SDF	--
Smiles	CCN(CC)CCCC(C)NC1=C2C=CC(=CC2=NC=C1)Cl
Storage (From the date of receipt)	3 years -20°C powder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years -20°C powder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 64 mg/mL ( (200.08 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 64 mg/mL

Water : Insoluble

Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.

In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Dilution Calculator Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):