Chloroquine (NSC-187208)

Catalog No.S6999 Synonyms: Aralen, CHQ, CQ

For research use only.

Chloroquine (NSC-187208, Aralen, CHQ, CQ) is an antimalarial drug and autophagy/lysosome inhibitor. Chloroquine also suppresses Toll-like receptor-9 (TLR9) protein expression. Chloroquine is highly effective agianst SARS-CoV-2 (COVID-19) infection with EC50 of 1.13 μM in Vero E6 cells. Chloroquine has anti-HIV-1 activity.

Chloroquine (NSC-187208) Chemical Structure

CAS No. 54-05-7

Selleck's Chloroquine (NSC-187208) has been cited by 49 publications

Purity & Quality Control

Choose Selective Autophagy Inhibitors

Biological Activity

Description Chloroquine (NSC-187208, Aralen, CHQ, CQ) is an antimalarial drug and autophagy/lysosome inhibitor. Chloroquine also suppresses Toll-like receptor-9 (TLR9) protein expression. Chloroquine is highly effective agianst SARS-CoV-2 (COVID-19) infection with EC50 of 1.13 μM in Vero E6 cells. Chloroquine has anti-HIV-1 activity.
Targets
Autophagy [1] lysosome [1] TLR9 [2] HIV-1 [4] SARS-CoV-2 [3]
(Vero E6 cells based assay)
1.13 μM(EC50)
In vitro

Chloroquine is a widely used autophagy inhibitor.[5]

In vivo

The inhibition of autophagy by chloroquine potentiates the therapeutic effect of PI3K/AKT inhibitors in vitro and in vivo TNBC models, synergizing with taxane-based chemotherapy.[6]

Protocol (from reference)

Cell Research:

[5]

  • Cell lines: HL‐60 cells
  • Concentrations: 10 µM
  • Incubation Time: 48 h
  • Method:

    One day before treatment, AML cells were seeded with a density of 2 × 105 cells/mL. Fresh medium containing different concentrations of Ara‐C was added into the culture medium. Cells were further cultured for 24 or 48 h based on experimental purposes. For inhibition of autophagy, HL‐60 cells were treated with 200 nM bafilomycin A1 or 10 µM chloroquine. Overexpression and knockdown were performed by transfection.

    (Data sourced from selleck products)

Animal Research:

[6]

  • Animal Models: Female NOD/SCID athymic mice injected with MDAMB231 cells
  • Dosages: 30 mg/kg
  • Administration: o.g.

    (Data sourced from selleck products)

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 319.87
Formula

C18H26ClN3

CAS No. 54-05-7
Storage 3 years -20°C powder
2 years -80°C in solvent

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Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT05249361 Not yet recruiting Other: no intervention Duchenne Muscular Dystrophy Samsung Medical Center September 1 2022 --
NCT05071586 Not yet recruiting Device: Soterix 1x1 tDCS mini-CT Stimulator device Cerebral Palsy (CP) University of Wisconsin Madison August 2022 Not Applicable
NCT05452174 Enrolling by invitation Drug: Mirtazapine Pregnancy|Severe Nausea and Vomiting|Hyperemesis Gravidarum Northwestern University|Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD) June 13 2022 Phase 1|Phase 2

(data from https://clinicaltrials.gov, updated on 2022-08-01)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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