Chloroquine

Catalog No.S6999 Batch:S699901

Technical Data

Formula

C₁₈H₂₆ClN₃

Molecular Weight

319.87

CAS No.

54-05-7

Solubility (25°C)*

In vitro

DMSO

64 mg/mL (200.08 mM)

Ethanol

64 mg/mL (200.08 mM)

Water

Insoluble

In vivo (Add solvents to the product individually and in order)

Homogeneous suspension

CMC-NA

≥5mg/ml

Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

Chloroquine is an antimalarial drug and autophagy/lysosome inhibitor. Chloroquine also suppresses Toll-like receptor-9 (TLR9) protein expression. Chloroquine is highly effective agianst SARS-CoV-2 (COVID-19) infection with EC50 of 1.13 μM in Vero E6 cells. Chloroquine has anti-HIV-1 activity.

Targets

Autophagy ^[1]	lysosome ^[1]	TLR9 ^[2]	HIV-1 ^[4]	SARS-CoV-2 ^[3] (Vero E6 cells based assay)
				1.13 μM(EC50)

In vitro

Chloroquine is a widely used autophagy inhibitor.

In vivo

The inhibition of autophagy by chloroquine potentiates the therapeutic effect of PI3K/AKT inhibitors in vitro and in vivo TNBC models, synergizing with taxane-based chemotherapy.

Protocol (from reference)

Cell Assay:^{^[5]}	Cell lines HL‐60 cells Concentrations 10 µM Incubation Time 48 h Method One day before treatment, AML cells were seeded with a density of 2 × 10⁵ cells/mL. Fresh medium containing different concentrations of Ara‐C was added into the culture medium. Cells were further cultured for 24 or 48 h based on experimental purposes. For inhibition of autophagy, HL‐60 cells were treated with 200 nM bafilomycin A1 or 10 µM chloroquine. Overexpression and knockdown were performed by transfection. (Data sourced from selleck products)
Animal Study:^{^[6]}	Animal Models Female NOD/SCID athymic mice injected with MDAMB231 cells Dosages 30 mg/kg Administration o.g. (Data sourced from selleck products)

Cell Assay:^{^[5]}

Cell lines
HL‐60 cells
Concentrations
10 µM
Incubation Time
48 h
Method
One day before treatment, AML cells were seeded with a density of 2 × 10⁵ cells/mL. Fresh medium containing different concentrations of Ara‐C was added into the culture medium. Cells were further cultured for 24 or 48 h based on experimental purposes. For inhibition of autophagy, HL‐60 cells were treated with 200 nM bafilomycin A1 or 10 µM chloroquine. Overexpression and knockdown were performed by transfection.

(Data sourced from selleck products)

Animal Study:^{^[6]}

Animal Models
Female NOD/SCID athymic mice injected with MDAMB231 cells
Dosages
30 mg/kg
Administration
o.g.

(Data sourced from selleck products)

References

https://pubmed.ncbi.nlm.nih.gov/25385822/
https://pubmed.ncbi.nlm.nih.gov/24273604/
https://pubmed.ncbi.nlm.nih.gov/32145363/

https://pubmed.ncbi.nlm.nih.gov/11166661/
https://pubmed.ncbi.nlm.nih.gov/33638615/
https://pubmed.ncbi.nlm.nih.gov/35761360/

+ Expand

Selleck's Chloroquine Has Been Cited by 261 Publications

Indole-3-Propionic Acid Improves Alveolar Development Impairment via Targeting VAMP8-mediated SNAREs Complex Formation in Bronchopulmonary Dysplasia [ Adv Sci (Weinh), 2026, 13(19):e02610]	PubMed: 41650278
RPS6KA3/RSK2-mediated phosphorylation of DRAM2 promotes lysosomal targeting and autophagic flux in melanoma [ Autophagy, 2026, 1-20.]	PubMed: 42059423
ADCY7 dictates N-cadherin stability to inhibit HCC metastasis [ JHEP Rep, 2026, 8(6):101789]	PubMed: 42096912
LncRNA SNHG1 Knockdown Ameliorates HIV-1 gp120V3 Loop-Induced Microglial Neuroinflammation by Regulating the Autophagy Process [ Inflammation, 2026, 49(1):26]	PubMed: 41489751
The E3 ubiquitin ligase NEDD4 protects against nonesterified fatty acid-induced hepatic inflammatory injury in ketotic cows [ J Dairy Sci, 2026, 109(5):5594-5610]	PubMed: 41724477
Macromolecule-Loaded Hybrid Extracellular Vesicles via Ionic Lipid-Based Post-Loading for Intracellular Delivery: Functional Evaluation for Neurodegenerative Therapy [ J Extracell Biol, 2026, 5(3):e70125]	PubMed: 42022851
VCP downstream metabolite glycerol-3-phosphate (G3P) inhibits CD8+T cells function in the HCC microenvironment [ Signal Transduct Target Ther, 2025, 10(1):26]	PubMed: 39848960
MAPK13 phosphorylates PHGDH and promotes its degradation via chaperone-mediated autophagy during liver injury [ Cell Discov, 2025, 11(1):15]	PubMed: 39962071
Cytosolic cytochrome c represses ferroptosis [ Cell Metab, 2025, S1550-4131(25)00149-4]	PubMed: 40233758
Rab27a regulates the transport of influenza virus membrane proteins to the plasma membrane [ Nat Commun, 2025, 16(1):6271]	PubMed: 40623997

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.