Home DNA Damage/DNA Repair DNA/RNA Synthesis inhibitor CeMMEC1 HCl For research use only.

CeMMEC1 HCl

CeMMEC1 is an N-methylisoquinolinone derivative that inhibits the second bromodomain of TAF1 (IC50=0.9 μM).

CeMMEC1 HCl Chemical Structure

CeMMEC1 HCl Chemical Structure

CAS: 2095432-53-2

Purity & Quality Control

Batch: S832801 DMSO] 23 mg/mL] false] Ethanol] 1 mg/mL] false] Water] Insoluble] false Purity: 99.74%
99.74

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Signaling Pathway

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DNA/RNA Synthesis Signaling Pathway

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Biological Activity

Description CeMMEC1 is an N-methylisoquinolinone derivative that inhibits the second bromodomain of TAF1 (IC50=0.9 μM).
Targets
TAF1 [1]
(Cell-based assay)
1.8 μM(Kd)
In vitro
In vitro CeMMEC1 only bound BRD4 very weakly but it shows high affinity for the bromodomains of CREBBP, EP300, BRD9 and the second bromodomain of TAF1[1].
Cell Research Cell lines REDS3 cells
Concentrations 10 μM
Incubation Time 24 h
Method

REDS3 cells were treated with 10 μM of every compound, and live-cell imaging pictures were taken to assess their ability to induce RFP expression 24 h later. From this primary screening 1,286 small molecules were selected as hits and rescreened in the follow-up part, in which REDS3 and WT KBM7 cells were treated in three-point dose response in order to exclude autofluorescent or toxic compounds. 80 small molecules were selected as hits and used to treat WT KBM7 cells and REDS3 cells in eight-point dose (twofold dilution, starting from 100 μM) response and three-point time course (24 h, 48 h and 72 h) to carefully select the best true hits (time- and dose-dependent RFP expression/no autofluorescence). UPLC-MS analysis was done to confirm purity and the correct mass of the small molecules selected; finally, 22 small molecules fulfilled the criteria as screening hits.

Chemical Information & Solubility

Molecular Weight 372.8 Formula

C19H17ClN2O4
CAS No. 2095432-53-2 SDF Download CeMMEC1 HCl SDF
Smiles CN1C=C(C2=CC=CC=C2C1=O)C(=O)NC3=CC4=C(C=C3)OCCO4.Cl
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 23 mg/mL ( (61.69 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 1 mg/mL

Water : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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