CAS No. 1370032-19-1
Batch:
Purity:99.57
Chemical Structure
Check the
CA-5f
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
| IUPAC Name | (3E,5E)-3-[(3,4-dimethoxyphenyl)methylidene]-5-(1H-indol-3-ylmethylidene)-1-methylpiperidin-4-one | |
|---|---|---|
| CAS Number | 1370032-19-1 | |
| Molecular Formula |
C24H24N2O3 |
|
| Molecular Weight (MW) | 388.46 | |
| SMILES | CN1CC(=CC2=CC(=C(C=C2)OC)OC)C(=O)C(=CC3=CNC4=CC=CC=C43)C1 | |
| InChIKey | JYOLPDWVAMBMQN-UBIAKTOFSA-N |
CA-5f is a synthetic late-stage macroautophagy inhibitor that targetedly disrupts autophagosome-lysosome fusion. By blocking the final clearance step in the autophagic pathway, CA-5f leads to the accumulation of autophagosomes and autophagic substrates, impairing basal autophagic flux. This inhibition of downstream autophagic degradation results in compromised cellular homeostasis and enhanced cell sensitivity to metabolic and proteotoxic stress. [1]
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Concentration
Volume
Mass
|
|---|
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
78 mg/mL
(200.79 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | Insoluble |
| Ethanol | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.