C75 trans

Catalog No.S8915 Batch:S891502

Technical Data

Formula

C₁₄H₂₂O₄

Molecular Weight

254.32

CAS No.

191282-48-1

Solubility (25°C)*

In vitro

DMSO

51 mg/mL (200.53 mM)

Ethanol

12 mg/mL (47.18 mM)

Water

Insoluble

In vivo (Add solvents to the product individually and in order)

Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	2.5mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 50 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil	1.26mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 25.2 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

C75 trans (trans-C75, (±)-C75) is a novel, potent synthetic inhibitor of fatty acid synthase (FAS/FASN) with IC50 of 35 μM and 50 μM in clonogenic assays and spheroid growth assay, respectively. C75 is used as a tool for studying fatty acid synthesis in metabolic disorders and cancer.

Targets

FASN ^[1] (clonogenic assay)	FASN ^[1] (spheroid growth assay)
35 μM	50 μM

In vitro

C75 is toxic to prostate cancer cells. C75 reduces cell migration, while enhances radiation-induced clonogenic kill and enhances the spheroid growth delay induced by radiation exposure. Apoptosis induction by radiation exposure is enhanced by C75 treatment.^[1] C75 inhibits fatty acid synthesis and growth of established cancer cell lines from neu-N transgenic mice. C75 reduces the proliferation and expression of FAS, neu, and related genes in mammary epithelium.^[2]

In vivo

C75 inhibits fatty acid synthesis and in vivo mammary cancer development. C75 treatment prevents mammary cancer development and retards mammary development in neu-N transgenic mice.^[2]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines PC3 cells, LNCaP cells Concentrations 5 μM, 25 μM, 35 μM, 50 μM Incubation Time 3 h, 6 h, 24 h Method PC3 cells are seeded in 25 cm² flasks at 10⁵ cells/flask. When cultures are in exponential growth phase, media is removed and replaced with fresh drug-containing media. Cells are then incubated for 24 h at 37℃ in 5% CO₂. To determine optimal sequencing of therapeutic agents, three different combination treatment schedules are assessed: 1. radiation and C75 administered simultaneously; 2. radiation administered 24 h before C75; and 3. radiation administered 24 h after C75. After treatment, cells are seeded for clonogenic survival assay.
Animal Study:^{^[2]}	Animal Models neu-N transgenic mice, FVB/N mice Dosages 30mg/kg Administration IP

Cell Assay:^{^[1]}

Cell lines
PC3 cells, LNCaP cells
Concentrations
5 μM, 25 μM, 35 μM, 50 μM
Incubation Time
3 h, 6 h, 24 h
Method
PC3 cells are seeded in 25 cm² flasks at 10⁵ cells/flask. When cultures are in exponential growth phase, media is removed and replaced with fresh drug-containing media. Cells are then incubated for 24 h at 37℃ in 5% CO₂. To determine optimal sequencing of therapeutic agents, three different combination treatment schedules are assessed: 1. radiation and C75 administered simultaneously; 2. radiation administered 24 h before C75; and 3. radiation administered 24 h after C75. After treatment, cells are seeded for clonogenic survival assay.

Animal Study:^{^[2]}

Animal Models
neu-N transgenic mice, FVB/N mice
Dosages
30mg/kg
Administration
IP

References

Selleck's C75 trans has been cited by 2 publications

Protein C receptor maintains cancer stem cell properties via activating lipid synthesis in nasopharyngeal carcinoma [ Signal Transduct Target Ther, 2022, 7(1):46]	PubMed: 35169126
Pharmacological inhibition of fatty acid synthesis blocks SARS-CoV-2 replication [ Nat Metab, 2021, 10.1038/s42255-021-00479-4]	PubMed: 34580494

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.