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β-nicotinamide mononucleotide (NMN) Neuroprotective/Anti-Aging agent

Name: β-nicotinamide mononucleotide (NMN)
Brand: Selleck Chemicals
SKU: S5259
Availability: InStock
Rating: 5 (26 reviews)

Cat.No.S5259

Nicotinamide mononucleotide (β-NM, NMN), one of the key precursors of NAD+ and products of the NAMPT reaction , is converted to NAD+ by nicotinamide mononucleotide adenylyltransferase.

Chemical Structure

Molecular Weight: 334.22

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Quality Control

Batch: Purity: 99.93%

99.93

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Related Targets	Dehydrogenase HSP Transferase P450 (e.g. CYP17) PDE phosphatase PPAR Vitamin Carbohydrate Metabolism Mitochondrial Metabolism
Other Endogenous Metabolite Inhibitors	Heparan Sulfate 4-HNE (4-Hydroxynonenal) Ademetionine (S-Adenosylmethionine) Sodium L-lactate

Cell Culture, Treatment & Working Concentration

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
HL60 cells	Function assay	80 mM		syrosingopine-metformin-treated HL60 cells grown in medium supplemented with NMN had a partial recovery in ATP levels	30540938
MC3T3-E1	Cell viability assay	0.1, 1, 10 mM	6 h	AlCl3-induced reduction of MC3T3-E1 cell viability was successfully recovered by NMN treatment.	30292833
HepG2	Cell viability assay	0.5 mM	48 h	NMN was able to restore reduced NAD concentrations but not cell viability after stimulation with palmitate.	28974242
Click to View More Cell Line Experimental Data

Solubility

In vitro
Batch:

Water : 67 mg/mL

DMSO : Insoluble
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Clear solution	5%H2O 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	3.350mg/ml (10.02mM)	Taking the 1 mL working solution as an example, add 50 μL 67 mg/ml clarified H20 stock solution to 400 μL PEG300, mix evenly to clarify it; add 50 μL Tween80 to the above system, mix evenly to clarify it; then continue to add 500 μL ddH2O to adjust the volume to 1 mL. . The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	334.22	Formula	C₁₁H₁₅N₂O₈P	Storage (From the date of receipt)	3 years -20°C powder
CAS No.	1094-61-7	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	β-NM, NMN			Smiles	C1=CC(=C[N+](=C1)C2C(C(C(O2)COP(=O)(O)[O-])O)O)C(=O)N

Mechanism of Action

In vitro	Nicotinamide mononucleotide, the NAD+ precursor, rescues the NAD+/NADH ratio and exerts anti-senescent effects by activating SIRT1.
In vivo	NMN reduces the loss of cochlear hair cells caused by cisplatin and improves hearing in C57BL/6 mice, by supplementation of NAD+ through de novo and salvage synthesis pathways.
References	[1] https://pubmed.ncbi.nlm.nih.gov/33609723/ [2] https://pubmed.ncbi.nlm.nih.gov/34089817/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2024-05-22)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT06036355	Not yet recruiting	Postoperative Prevention of Tumor	Shanghai Cell Therapy Group Co.Ltd	September 30 2023	Early Phase 1
NCT05759468	Recruiting	Type2diabetes\|Diabetic Kidney Disease	Brigham and Women''s Hospital\|Boston Medical Center	April 13 2023	Phase 2
NCT05789355	Recruiting	Sickle Cell Disease	LGD\|CEN Biotech\|Assistance Publique Hopitaux De Marseille\|Etablissement Français du Sang	April 1 2023	Not Applicable
NCT04910061	Completed	Healthy Volunteers	Seneque SA\|Dicentra Inc.\|LGD	August 5 2021	Not Applicable

Tech Support

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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