Name: Oleic Acid
Brand: Selleck Chemicals
SKU: S4707
Rating: 5 (6 reviews)

Oleic acid (Oleate, 9-Octadecenoic Acid, Elaidoic acid) is a fatty acid that occurs naturally in various animal and vegetable fats and oils. It is used commercially in the preparation of oleates and lotions, and as a pharmaceutical solvent. Oleic acid is an abundant monounsaturated fatty acid. Oleic acid is a Na+/K+ ATPase activator.

Oleic Acid Chemical Structure

CAS: 112-80-1

Selleck's Oleic Acid has been cited by 6 publications

Purity & Quality Control

Batch: Purity: 99.85%

99.85

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Signaling Pathway

ATPase Signaling Pathway

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Biological Activity

Description	Oleic acid (Oleate, 9-Octadecenoic Acid, Elaidoic acid) is a fatty acid that occurs naturally in various animal and vegetable fats and oils. It is used commercially in the preparation of oleates and lotions, and as a pharmaceutical solvent. Oleic acid is an abundant monounsaturated fatty acid. Oleic acid is a Na+/K+ ATPase activator.

In vitro
In vitro	Oleic acid treatment induces a marked increase in the proliferation of 786-O cells in a dose-dependent manner. It delays 786-O cell apoptosis. In 786‑O cells, ILK is upregulated by oleic acid treatment. Oleic acid activates G protein-coupled receptors and phosphorylates ERK1/2 to induce cancer cell proliferation in breast cancer^[1]. Oleic acid(as low as 5 M) inhibits the stearic acid-induced increase in intercellular adhesion molecule-1 (ICAM-1) expression. Oleic acid is able to diminish endothelial cell activation, irrespective of the stimulus utilized, by suppressing the upregulation of adhesion molecules. It can protect Chinese hamster ovary cells from palmitate-induced lipotoxicity by promoting triglyceride synthesis within the cells^[2].
Cell Research	Cell lines	Human RCC（renal cell carcinoma） cell line, 786-O.
	Concentrations	0.05, 0.1, 0.2 mmol/l
	Incubation Time	48 h
	Method	Cells (2x10³ cells/well) are seeded in 96-well microtitre plates and incubated for 24 h in 100 µl culture medium. Cells in the experimental group were then treated with 0.05, 0.1 or 0.2 mmol/l oleic acid for 48 h. MTT [20 µl (5 g/l)] is added to the cells which were then cultivated for a further 4 h. Following the removal of the supernatant fluid, 150 µl/well DMSO is added to the cells which are agitated for 15 min. Absorbance is measured at 490 nm by an ELISA reader.

In Vivo
In vivo	Oleic acid inhibits in vivo Na/K-ATPase activity in the lungs and induced lung injury. 10 μM oleic acid induces lung edema and inflammation with cell influx, lipid body formation, and leukotriene B4 (LTB4) and prostaglandin E2 (PGE2) production^[3]. Oleic acid ameliorates amyloidosis in mouse models of Alzheimer's disease^[4].
Animal Research	Animal Models	male Swiss mice
	Dosages	2.5, 5.0 or 10.0 μmol
	Administration	i.v.

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-01-11)
NCT05556967	Recruiting	Adherence Patient	University of Mississippi Medical Center\|University of Mississippi National Center for Natural Products Research\|National Center for Complementary and Integrative Health (NCCIH)	August 9 2023	Not Applicable
NCT04614233	Active not recruiting	Overweight and Obesity	Yale University\|National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)	April 6 2021	Phase 4
NCT04053569	Active not recruiting	Metabolic Syndrome Protection Against	Azienda Ospedaliera Specializzata in Gastroenterologia Saverio de Bellis	October 1 2019	Not Applicable
NCT05791370	Completed	Diet\|Healthy	Malaysia Palm Oil Board\|Universiti Putra Malaysia	January 1 2019	Not Applicable
NCT04832217	Completed	Body Weight Changes\|Habits Diet\|Habit Food\|Life Change Events	Universidad de Zaragoza	January 20 2019	Not Applicable

References

[4] Amtul Z, et al. Brain Pathol. 2011, 21(3):321-9.

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Chemical Information & Solubility

Molecular Weight	282.46	Formula	C₁₈H₃₄O₂
CAS No.	112-80-1	SDF	Download Oleic Acid SDF
Density	0.89 g/mL at 25 °C
Smiles	CCCCCCCCC=CCCCCCCCC(=O)O
Storage (From the date of receipt)	2 years -80°C liquid	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	2 years -80°C liquid		1 month-20°Cin solvent

In vitro
Batch:

Ethanol : 100 mg/mL

DMSO : 50 mg/mL ( (177.01 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.

In vivo Formulation Calculator

Preparing Stock Solutions

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In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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