BC1618

For research use only.

Catalog No.S9860

BC1618 Chemical Structure

CAS No. 2222094-18-8

BC1618 is an orally active Fbxo48 inhibitor that stimulates adenosine monophosphate (AMP)-activated protein kinase (Ampk)-dependent signaling. BC1618 promotes mitochondrial fission, facilitates autophagy and improves hepatic insulin sensitivity.

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Biological Activity

Description BC1618 is an orally active Fbxo48 inhibitor that stimulates adenosine monophosphate (AMP)-activated protein kinase (Ampk)-dependent signaling. BC1618 promotes mitochondrial fission, facilitates autophagy and improves hepatic insulin sensitivity.
Targets
Fbxo48 [1]
()
In vitro

BC1618 interrupts Fbxo48/pAmpkα interaction. BC1618 facilitates mitochondrial fission and autophagy.[1]

In vivo

Consistent with augmenting Ampk activity, BC1618 promotes mitochondrial fission, facilitates autophagy and improves hepatic insulin sensitivity in high-fat-diet-induced obese mice.[1]

Protocol

Cell Research:

[1]

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  • Cell lines: 293T cells
  • Concentrations: 3 μM
  • Incubation Time: 30 min
  • Method:

    293T cells transfected with Fbxo48-V5 or Fbxo30-V5 are treated with control (DMSO) or BC1618 (3 μM) for 30 min. Cells are then washed and equally fractioned into 10 aliquots and subjected to a range of increasing temperatures to promote protein denaturation. After a high-speed spin to precipitate heat-denatured proteins, the soluble protein fraction from each temperature is then immunoblotted for Fbxo48 or Fbxo30.


    (Only for Reference)
Animal Research:

[1]

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  • Animal Models: C57BL/6 mice
  • Dosages: 15 mg/kg, 20 mg/kg, 30 mg/kg
  • Administration: Oral gavage
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 83 mg/mL (199.78 mM)
Water Insoluble
Ethanol '''''83 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 415.45
Formula

C24H24F3NO2

CAS No. 2222094-18-8
Storage powder
in solvent
Synonyms N/A

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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AMPK Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID