BC1618

Catalog No.S9860 Batch:S986001

Technical Data

Formula

C₂₄H₂₄F₃NO₂

Molecular Weight

415.45

CAS No.

2222094-18-8

Solubility (25°C)*

In vitro

DMSO

83 mg/mL (199.78 mM)

Ethanol

83 mg/mL (199.78 mM)

Water

Insoluble

In vivo (Add solvents to the product individually and in order)

Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 95 %Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.59mg/ml (1.42mM)	Taking the 1 mL working solution as an example, add 50 μL of 11.8 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	4.15mg/ml (9.99mM)	Taking the 1 mL working solution as an example, add 50 μL of 83 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

BC1618 is an orally active Fbxo48 inhibitor that stimulates adenosine monophosphate (AMP)-activated protein kinase (Ampk)-dependent signaling. This compound promotes mitochondrial fission, facilitates autophagy and improves hepatic insulin sensitivity.

Targets

Fbxo48 ^[1]

In vitro

This compound interrupts Fbxo48/pAmpkα interaction. It facilitates mitochondrial fission and autophagy.

In vivo

Consistent with augmenting Ampk activity, BC1618 promotes mitochondrial fission, facilitates autophagy and improves hepatic insulin sensitivity in high-fat-diet-induced obese mice.

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines 293T cells Concentrations 3 μM Incubation Time 30 min Method 293T cells transfected with Fbxo48-V5 or Fbxo30-V5 are treated with control (DMSO) or BC1618 (3 μM) for 30 min. Cells are then washed and equally fractioned into 10 aliquots and subjected to a range of increasing temperatures to promote protein denaturation. After a high-speed spin to precipitate heat-denatured proteins, the soluble protein fraction from each temperature is then immunoblotted for Fbxo48 or Fbxo30.
Animal Study:^{^[1]}	Animal Models C57BL/6 mice Dosages 15 mg/kg, 20 mg/kg, 30 mg/kg Administration Oral gavage

Cell Assay:^{^[1]}

Cell lines
293T cells
Concentrations
3 μM
Incubation Time
30 min
Method
293T cells transfected with Fbxo48-V5 or Fbxo30-V5 are treated with control (DMSO) or BC1618 (3 μM) for 30 min. Cells are then washed and equally fractioned into 10 aliquots and subjected to a range of increasing temperatures to promote protein denaturation. After a high-speed spin to precipitate heat-denatured proteins, the soluble protein fraction from each temperature is then immunoblotted for Fbxo48 or Fbxo30.

Animal Study:^{^[1]}

Animal Models
C57BL/6 mice
Dosages
15 mg/kg, 20 mg/kg, 30 mg/kg
Administration
Oral gavage

References

https://pubmed.ncbi.nlm.nih.gov/33495648/

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.