Indomethacin

For research use only.

Catalog No.S1723

9 publications

Indomethacin  Chemical Structure

CAS No. 53-86-1

Indomethacin is a nonselective COX1 and COX2 inhibitor with IC50 of 0.1 μg/mL and 5 μg/mL, respectively, used to reduce fever, pain, stiffness, and swelling.

Size Price Stock Quantity  
10mM (1mL in DMSO) USD 130 In stock
USD 97 In stock
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Selleck's Indomethacin has been cited by 9 publications

1 Customer Review

  • Indicated PN and MES spheres were shown expressing GFP under the control of a 7 × TCF/LEF optimal promoter cassette (7 × TOP) and constitutively expressed nuclear mCherry. PN 84 and MES 1123 were treated with Wnt3a (200 ng/ml), indomethacin (Indo; 20 μM), or a control (vehicle). Scale bar, 100 μm. Bar graph, quantification of fluorescent signal of GFP (TOP-GFP) versus mCherry (PGK-H2BmCherry). The percentage of GFP- and mCherry-positive spheres was determined by FACS.

    Nat Commun, 2016, 7:12885. Indomethacin purchased from Selleck.

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Biological Activity

Description Indomethacin is a nonselective COX1 and COX2 inhibitor with IC50 of 0.1 μg/mL and 5 μg/mL, respectively, used to reduce fever, pain, stiffness, and swelling.
Targets
COX1 [6] COX-2 [6]
0.28 μM 14 μM
In vitro

Indomethacin inhibits transcription of a beta-catenin/TCF-responsive reporter gene in a dose dependent manner. Indomethacin also downregulates the beta-catenin/TCF transcriptional target cyclin D1. Indomethacin attenuates the transcription of beta-catenin/TCF-responsive genes, by modulating TCF activity without disrupting beta-catenin/TCF complex formation. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HeLa cells NGnRUYtHfW6ldHnvckBie3OjeR?= MVGyNEBucW6| M160OGFod26rc4SgZYN1cX[rdImgZZQh[W6mcn;n[Y4hemWlZYD0c5IhMHWwa37ve44hd3KrZ3nuLUBmgHC{ZYPz[YQhcW5iSHXMZUBk\WyuczDjc{1mgHC{ZYPzbY5oKFCVQT2oRXJGMTRvTIXjNVMh[XO|ZYPz[YQh[XNiaX7keYN1cW:wIH;mJGRJXC2rbnT1Z4VlKGy3Y3nm[ZJie2ViYXP0bZZqfHliYX\0[ZIhOjBibXnud{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYmsJGVEPTB;MD6xOEBvVQ>? MorQNlM1OzJyOUW=
J774 cells NGjIbZFHfW6ldHnvckBie3OjeR?= NWHZbXhnUW5idnn0do8hcW6qaXLpeI9zgSCjY4Tpeol1gSCjZ3HpcpN1KFC{b4P0ZYdt[W6maX6gS{9JKHO7boToZZNmKDFiaX6gcZVzcW6nIFq3O|Qh[2WubIOsJGlEPTB;MjDuUS=> MUexOVg2PzF2OR?=
U-937 cells NHfyXYlHfW6ldHnvckBie3OjeR?= M2OxfGlvKH[rdILvJIlvcGmkaYTvdpkh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDQdo9{fGGpbHHu[IlvKEdxSDDzfY51cGG|ZTCxJEhEV1hvMTmgbY4hXS17M{egZ4VtdHNuIFnDOVA:OiCwTR?= MmjoNVI5Pzd3OES=
rat synovial cells MoKySpVv[3Srb36gZZN{[Xl? NYDGeHdHUW5idnn0do8hcW6qaXLpeI9zgSCnZn\lZ5Qhd25icILv[JVkfGmxbjDv[kBxem:|dHHncIFv\GmwIFWyJEhRT0V{KTDpckBz[XRic4nuc5Zq[WxiY3XscJMtKEmFNUC9Nk46KG6P MlPiNVA5OjF5MU[=
143982 cells MluwSpVv[3Srb36gZZN{[Xl? M4HJXWlvKH[rdILvJIlvcGmkaYTvdpkh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDQdo9{fGGpbHHu[IlvKEdxSDDzfY51cGG|ZTCyJEhEV1hvMjmgbY4hOTR|OUiyJINmdGy|LDDJR|UxRTlibl2= NYTQW4UzOTJ6N{e1PFQ>
CHO cells NFTZNHBHfW6ldHnvckBie3OjeR?= M1Xu[GlvKH[rdILvJIlvcGmkaYTvdpkheG:2ZX7jfUBi\2GrboP0JIh2dWGwIFPPXE0yKGmwIIP0ZYJtgSC2cnHud4Zm[3SnZDDjbIlv\XOnIHjhcZN1\XJib4\hdpkhMEOKTzmgZ4VtdHNuIFnDOVA:OThibl2= NEfVZYoyODV5Nk[4OC=>
RBL-1 cell line M3X0OGZ2dmO2aX;uJIF{e2G7 NY\4[o1oUW6qaXLpeIlwdiCxZjDQdo9{fGGpbHHu[IlvKEdxSDDzfY51cGG|ZTDpckBqdnSjY4SgVmJNNTFiY3XscEBtcW6nLDDJR|UxRTBwNTFOwG0> NIHCUYMzOTF3NUi2
SF-9 insect cells NH[2OXhHfW6ldHnvckBie3OjeR?= M17JeVIxKE2rboO= NVzOe2x5UW6qaXLpeIlwdiCxZjDoeY1idiCFT2iyJIV5eHKnc4Pl[EBqdiCVRj25JIlve2WldDDj[YxteyCjc4Pld5Nm\CCjczDjc453\XK|aX;uJI9nKFtzNFPdMYFz[WOqaXTvcolkKGGlaXSgeI8hYzF2Q22tdJJwe3SjZ3zhcoRqdnNicILlbY5kfWKjdHXkJIZweiB{MDDtbY5{KGK7IITobY4udGG7ZYKgZ4hzd22jdH;ndoFxcHliYX7hcJl{cXNuIFnDOVA:OC55NTFOwG0> NEHhR5czOTV{NEW4Oy=>
mouse RAW264.7 cells MV;GeY5kfGmxbjDhd5NigQ>? NIe3VG4zPCCq NYrRR3J5SW62aXnu[oxidW2jdH;yfUBi[3Srdnn0fUBqdiCvb4Xz[UBTSVd{NkSuO{Bk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIFzQV{1qdmS3Y3XkJG5QKHC{b3T1Z5Rqd25iYX\0[ZIhOjRiaILzJIJ6KEe{aXXzd{Bie3OjeTygTWM2OD1zLkK1JO69VQ>? M1fZO|I1QDB4M{Gw
mouse BV2 cells MV;GeY5kfGmxbjDhd5NigQ>? Mmn4NlQhcA>? M3Wz[mFvfGmrbn\sZY1u[XSxcomgZYN1cX[rdImgbY4hdW:3c3WgRnYzKGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gUHBUNWmwZIXj[YQhVk9icILv[JVkfGmxbjDh[pRmeiB{NDDodpMh[nliR4Lp[ZN{KGG|c3H5MEBKSzVyPUeuNUDPxE1? NWHPWlY1OjRyNEKwNFc>
human 2008 cells NWDNWnNSTnWwY4Tpc44h[XO|YYm= NHLxVGpKdmirYnn0bY9vKG:oIHj1cYFvKE2UUEGgbY4hcHWvYX6gNlAxQCClZXzsd{whUUN3ME2xNkDPxE1? NXj3TpcyOTh5MEe4PFQ>
A549 cells  NFrV[HRHfW6ldHnvckBie3OjeR?= MUKxNEDPxE1? MX;Jcohq[mm2aX;uJI9nKG2SR1XTNUBqdiCqdX3hckBCPTR7IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhUUxvMT3i[ZRiNWmwZIXj[YQhWEeHMjDmc5Ju[XSrb36gZZQhOTBidV2gZpkh[2WubD3pcpRi[3RiYYPzZZk> M2[wXVE6ODV|N{Wx
HepG2 cells M17odmZ2dmO2aX;uJIF{e2G7 NHX4OG0yODBizszN MV;Jcohq[mm2aX;uJI9nKE2UUEGtcYVlcWG2ZXSg[I95d3K3YnnjbY4h\W[obIX4JIlvKGSxeH;yeYJq[2mwIILld4l{fGGwdDDoeY1idiCKZYDHNkBk\WyuczDheEAyODBidV2gZpkh\myxdzDjfZRwdWW2com= NUnqUpVsOjB2NUW1O|g>
THP1 cells M2noN2Z2dmO2aX;uJIF{e2G7 NF:z[5EyODBizszN MWqzNEBucW6| NVzRV3FnUXK{ZY\ldpNq[mynIHnubIljcXSrb36gc4YhS0:[LUGgbY4hcHWvYX6gWGhROSClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJIFz[WOqaXTvcolkKGGlaXStbY5lfWOnZDDUXGIzKG[xcn3heIlwdiCjdDCxNFAhfU1iaX7jeYJifGWmIH\vdkA{OCCvaX7zJIZwdGyxd3XkJIJ6KGOxbYDveY5lKHejc3jveZQhdWWjc4Xy[YQhOzBibXnud{Bxd3O2IHHyZYNpcWSxbnnjJIFkcWRiY3jhcIxmdmenIHL5JJJi\GmxaX3teY5w[XO|YYm= M1;tbFI{PjVzM{W5
MDA-MB-231 cells MVXGeY5kfGmxbjDhd5NigQ>? MXOxNFAh|ryP MYqzNEBucW6| NXrqWI9kUXK{ZY\ldpNq[mynIHnubIljcXSrb36gc4YhS0:[LUGgbY4hcHWvYX6gUWRCNU2ELUKzNUBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIHHyZYNpcWSxbnnjJIFkcWRvaX7keYNm\CCSR1WyJIZwem2jdHnvckBifCBzMECgeW0hcW6ldXLheIVlKG[xcjCzNEBucW6|IH\vcIxwf2WmIHL5JINwdXCxdX7kJJdie2ixdYSgcYVie3W{ZXSgN|AhdWmwczDwc5N1KGG{YXPobYRwdmmlIHHjbYQh[2ijbHzlcodmKGK7IILh[IlwcW2vdX7vZZN{[Xl? MYOyN|Y2OTN3OR?=
LNCAP cells NI\VXI5HfW6ldHnvckBie3OjeR?= Ml\aN|Ah|ryP NFz6c|M1QCCq M2PJOWlvcGmkaYTpc44hd2ZiQVvSNWM{KCi3bnvuc5dvKG:{aXfpckkudWWmaXH0[YQhfGW|dH;zeIVzd26nLUG3ZoV1[S2pbIXjeZJwdmmmZTDmc5Ju[XSrb36g[ZhxemW|c3XkJIlvKGi3bXHuJGxPS0GSIHPlcIx{KGG2IEOwJJVOKGGodHXyJFQ5KGi{czDpckBxemW|ZX7j[UBw\iB2LXHu[JJwe3SnbnWtN{wyPy2maX;u[S=> NFLZXXEzOzR|MkC5OS=>
mouse BV2 cells MUfDfZRwfG:6aXPpeJkh[XO|YYm= MV6yOEBp M4rifWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KG2xdYPlJGJXOiClZXzsd{Bie3Onc4Pl[EBieyCvYYjpcZVuKG6xbj30c5hq[yClb37j[Y51emG2aX;uJIFnfGW{IEK0JIhzeyCkeTDNWHQh[XO|YYm= MYKyN|cyOTlzNx?=

... Click to View More Cell Line Experimental Data

In vivo Indomethacin increases BrdU+ cells of all lineages and reduces microglial/monocyte activation in rats after focal cerebral ischemia. [2] Indomethacin (7.5 mg/kg, one injection) produces acute injury and inflammation in the distal jejunum and proximal ileum that are maximal at three days and completely resolves within one week. Indomethacin (two daily subcutaneous injections) produces a more extensive and chronic inflammation that lasts in an active form in more than 75% of the rats for at least two weeks. [3] Indomethacin (4ppm) reduces tumor yield by 78% in SKH:HR-1-hrBr hairless mice. [4] Indomethacin (20 mg/kg, orally) increases the total area of gastric erosions and concentration of lipid peroxides in the gastric mucosa of rats. Indomethacin increases the alpha-tocopherol:total cholesterol ratio in serum. Indomethacin inhibits the increases in gastric mucosal erosions and lipid peroxides in the gastric mucosa, and the reduction of serum alpha-tocopherol. [5]

Protocol

Solubility (25°C)

In vitro DMSO 72 mg/mL (201.23 mM)
Ethanol 24 mg/mL (67.07 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+30% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.
2mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 357.79
Formula

C19H16ClNO4

CAS No. 53-86-1
Storage powder
in solvent
Synonyms N/A
Smiles CC1=C(C2=C(N1C(=O)C3=CC=C(C=C3)Cl)C=CC(=C2)OC)CC(=O)O

In vivo Formulation Calculator (Clear solution)

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Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04025177 Not yet recruiting Drug: Indomethacin Injection Patent Ductus Arteriosus After Premature Birth University of Manitoba|St. Boniface Hospital|Health Sciences Centre Winnipeg Manitoba|University at Buffalo January 2020 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID