Indomethacin

Catalog No.S1723

Indomethacin  Chemical Structure

Molecular Weight(MW): 357.79

Indomethacin is a nonselective COX1 and COX2 inhibitor with IC50 of 0.1 μg/mL and 5 μg/mL, respectively, used to reduce fever, pain, stiffness, and swelling.

Size Price Stock Quantity  
In DMSO USD 130 In stock
USD 97 In stock
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Cited by 6 Publications

1 Customer Review

  • Indicated PN and MES spheres were shown expressing GFP under the control of a 7 × TCF/LEF optimal promoter cassette (7 × TOP) and constitutively expressed nuclear mCherry. PN 84 and MES 1123 were treated with Wnt3a (200 ng/ml), indomethacin (Indo; 20 μM), or a control (vehicle). Scale bar, 100 μm. Bar graph, quantification of fluorescent signal of GFP (TOP-GFP) versus mCherry (PGK-H2BmCherry). The percentage of GFP- and mCherry-positive spheres was determined by FACS.

    Nat Commun, 2016, 7:12885. Indomethacin purchased from Selleck.

Purity & Quality Control

Choose Selective COX Inhibitors

Biological Activity

Description Indomethacin is a nonselective COX1 and COX2 inhibitor with IC50 of 0.1 μg/mL and 5 μg/mL, respectively, used to reduce fever, pain, stiffness, and swelling.
Targets
COX1 [6] COX-2 [6]
0.28 μM 14 μM
In vitro

Indomethacin inhibits transcription of a beta-catenin/TCF-responsive reporter gene in a dose dependent manner. Indomethacin also downregulates the beta-catenin/TCF transcriptional target cyclin D1. Indomethacin attenuates the transcription of beta-catenin/TCF-responsive genes, by modulating TCF activity without disrupting beta-catenin/TCF complex formation. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HeLa cells NYjmbZlmTnWwY4Tpc44h[XO|YYm= NHW0UlczOCCvaX7z M2nuVmFod26rc4SgZYN1cX[rdImgZZQh[W6mcn;n[Y4hemWlZYD0c5IhMHWwa37ve44hd3KrZ3nuLUBmgHC{ZYPz[YQhcW5iSHXMZUBk\WyuczDjc{1mgHC{ZYPzbY5oKFCVQT2oRXJGMTRvTIXjNVMh[XO|ZYPz[YQh[XNiaX7keYN1cW:wIH;mJGRJXC2rbnT1Z4VlKGy3Y3nm[ZJie2ViYXP0bZZqfHliYX\0[ZIhOjBibXnud{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYmsJGVEPTB;MD6xOEBvVQ>? NFS5emIzOzR|MkC5OS=>
J774 cells MUTGeY5kfGmxbjDhd5NigQ>? MofzTY4hfmm2cn:gbY5pcWKrdH;yfUBi[3Srdnn0fUBi\2GrboP0JHBzd3O2YXfsZY5lcW5iRz;IJJN6dnSqYYPlJFEhcW5ibYXybY5mKEp5N{SgZ4VtdHNuIFnDOVA:OiCwTR?= Ml7QNVU5PTdzNEm=
U-937 cells NVLRS3ZnTnWwY4Tpc44h[XO|YYm= NFqybVJKdiC4aYTyc{BqdmirYnn0c5J6KGGldHn2bZR6KGGpYXnud5QhcHWvYX6gVJJwe3SjZ3zhcoRqdiCJL1igd5lvfGijc3WgNUApS0:[LUGpJIlvKFVvOUO3JINmdGy|LDDJR|UxRTJibl2= NULjN|V7OTJ6N{e1PFQ>
rat synovial cells M3TRRWZ2dmO2aX;uJIF{e2G7 M2\x[2lvKH[rdILvJIlvcGmkaYTvdpkh\W[oZXP0JI9vKHC{b3T1Z5Rqd25ib3[gdJJwe3SjZ3zhcoRqdiCHMjCoVGdGOiliaX6gdoF1KHO7bn;2bYFtKGOnbHzzMEBKSzVyPUKuPUBvVQ>? M3S1T|ExQDJzN{G2
143982 cells M3XES2Z2dmO2aX;uJIF{e2G7 MoTDTY4hfmm2cn:gbY5pcWKrdH;yfUBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGDyc5N1[WeuYX7kbY4hTy:KIIP5cpRp[XOnIEKgLGNQYC1{KTDpckAyPDN7OEKgZ4VtdHNuIFnDOVA:QSCwTR?= MorjNVI5Pzd3OES=
CHO cells MWfGeY5kfGmxbjDhd5NigQ>? NGPMU|NKdiC4aYTyc{BqdmirYnn0c5J6KHCxdHXuZ5kh[WejaX7zeEBpfW2jbjDDU3guOSCrbjDzeIFjdHlidILhcpNn\WO2ZXSgZ4hqdmW|ZTDoZY1{fGW{IH;2ZZJ6KCiFSF:pJINmdGy|LDDJR|UxRTF6IH7N MUKxNFU4PjZ6NB?=
RBL-1 cell line M3zuemZ2dmO2aX;uJIF{e2G7 MYDJcohq[mm2aX;uJI9nKFC{b4P0ZYdt[W6maX6gS{9JKHO7boToZZNmKGmwIHnueIFkfCCUQlytNUBk\WyuIHzpcoUtKEmFNUC9NE42KM7:TR?= MUmyNVE2PTh4
SF-9 insect cells MYHGeY5kfGmxbjDhd5NigQ>? MWOyNEBOcW6| MnLLTY5pcWKrdHnvckBw\iCqdX3hckBEV1h{IHX4dJJme3OnZDDpckBUTi17IHnud4VkfCClZXzsd{Bie3Onc4Pl[EBieyClb372[ZJ{cW:wIH;mJHsyPEOfLXHyZYNpcWSxbnnjJIFkcWRidH:gX|E1S11vcILvd5Ri\2yjbnTpcpMheHKnaX7jeYJifGWmIH\vdkAzOCCvaX7zJIJ6KHSqaX6tcIF6\XJiY3jyc41ifG:pcnHwbJkh[W6jbInzbZMtKEmFNUC9NE44PSEQvF2= NYC0OpBsOjF3MkS1PFc>
mouse RAW264.7 cells Mn;OSpVv[3Srb36gZZN{[Xl? Mn7BNlQhcA>? NIHUcHVCdnSraX7mcIFudWG2b4L5JIFkfGm4aYT5JIlvKG2xdYPlJHJCXzJ4ND63JINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiTGDTMYlv\HWlZXSgUm8heHKxZIXjeIlwdiCjZoTldkAzPCCqcoOgZpkhT3KrZYPzJIF{e2G7LDDJR|UxRTFwMkWg{txO NWizcW5SOjR6ME[zNVA>
mouse BV2 cells NY\DO3dXTnWwY4Tpc44h[XO|YYm= MXOyOEBp M1i0fWFvfGmrbn\sZY1u[XSxcomgZYN1cX[rdImgbY4hdW:3c3WgRnYzKGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gUHBUNWmwZIXj[YQhVk9icILv[JVkfGmxbjDh[pRmeiB{NDDodpMh[nliR4Lp[ZN{KGG|c3H5MEBKSzVyPUeuNUDPxE1? Ml\HNlQxPDJyMEe=
human 2008 cells NHzLdm9HfW6ldHnvckBie3OjeR?= MV3Jcohq[mm2aX;uJI9nKGi3bXHuJG1TWDFiaX6gbJVu[W5iMkCwPEBk\WyuczygTWM2OD1zMjFOwG0> MUixPFcxPzh6NB?=
A549 cells  MYfGeY5kfGmxbjDhd5NigQ>? MoTUNVAh|ryP M{e0RWlvcGmkaYTpc44hd2ZibWDHSXMyKGmwIHj1cYFvKEF3NEmgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCLTD2xMYJmfGFvaX7keYNm\CCSR1WyJIZwem2jdHnvckBifCBzMDD1UUBjgSClZXzsMYlvfGGldDDhd5NigQ>? M4\Y[|E6ODV|N{Wx
HepG2 cells MVzGeY5kfGmxbjDhd5NigQ>? MVuxNFAh|ryP NEHtWFNKdmirYnn0bY9vKG:oIF3SVFEudWWmaXH0[YQh\G:6b4L1ZolkcW5iZX\mcJV5KGmwIHTvfI9zfWKrY3nuJJJme2m|dHHueEBpfW2jbjDI[ZBIOiClZXzsd{BifCBzMECgeW0h[nliZnzve{BkgXSxbXX0dpk> M4SzOlIxPDV3NUe4
THP1 cells NVj2O5FwTnWwY4Tpc44h[XO|YYm= MVWxNFAh|ryP MnG5N|AhdWmwcx?= NIS5U2RKenKndnXyd4ljdGViaX7obYJqfGmxbjDv[kBEV1hvMTDpckBpfW2jbjDUTHAyKGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gZZJi[2irZH;ubYMh[WOrZD3pcoR2[2WmIGTYRlIh\m:{bXH0bY9vKGG2IEGwNEB2VSCrbnP1ZoF1\WRiZn;yJFMxKG2rboOg[o9tdG:5ZXSgZpkh[2:vcH;1coQhf2G|aH;1eEBu\WG|dYLl[EA{OCCvaX7zJJBwe3RiYYLhZ4hq\G:waXOgZYNq\CClaHHscIVv\2ViYomgdoFlcW:rbX31co9ie3OjeR?= M3;mUVI{PjVzM{W5
MDA-MB-231 cells Mn3QSpVv[3Srb36gZZN{[Xl? MYexNFAh|ryP NX:4UWo3OzBibXnudy=> MXjJdpJmfmW{c3nicIUhcW6qaXLpeIlwdiCxZjDDU3guOSCrbjDoeY1idiCPRFGtUWIuOjNzIHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4Yh[XKjY3jp[I9vcWNiYXPp[E1qdmS3Y3XkJHBITTJiZn;ycYF1cW:wIHH0JFExOCC3TTDpcoN2[mG2ZXSg[o9zKDNyIH3pcpMh\m:ubH;3[YQh[nliY3;tdI92dmRid3HzbI92fCCvZXHzeZJm\CB|MDDtbY5{KHCxc4SgZZJi[2irZH;ubYMh[WOrZDDjbIFtdGWwZ3WgZpkhemGmaX;pcY12dm:jc4PhfS=> NGP4SVMzOzZ3MUO1PS=>
LNCAP cells NYT4fFhDTnWwY4Tpc44h[XO|YYm= NVLrZoZTOzBizszN M2\G[FQ5KGh? M1f3bGlvcGmkaYTpc44hd2ZiQVvSNWM{KCi3bnvuc5dvKG:{aXfpckkudWWmaXH0[YQhfGW|dH;zeIVzd26nLUG3ZoV1[S2pbIXjeZJwdmmmZTDmc5Ju[XSrb36g[ZhxemW|c3XkJIlvKGi3bXHuJGxPS0GSIHPlcIx{KGG2IEOwJJVOKGGodHXyJFQ5KGi{czDpckBxemW|ZX7j[UBw\iB2LXHu[JJwe3SnbnWtN{wyPy2maX;u[S=> NGLLSXEzOzR|MkC5OS=>
mouse BV2 cells NV36cIVDS3m2b4TvfIlkcXS7IHHzd4F6 NYfZTXRKOjRiaB?= MWTDfZRwfG:6aXPpeJkh[WejaX7zeEBud3W|ZTDCWlIh[2WubIOgZZN{\XO|ZXSgZZMhdWG6aX31cUBvd25vdH;4bYMh[2:wY3XueJJifGmxbjDh[pRmeiB{NDDodpMh[nliTWTUJIF{e2G7 M4LBOFI{PzFzOUG3

... Click to View More Cell Line Experimental Data

In vivo Indomethacin increases BrdU+ cells of all lineages and reduces microglial/monocyte activation in rats after focal cerebral ischemia. [2] Indomethacin (7.5 mg/kg, one injection) produces acute injury and inflammation in the distal jejunum and proximal ileum that are maximal at three days and completely resolves within one week. Indomethacin (two daily subcutaneous injections) produces a more extensive and chronic inflammation that lasts in an active form in more than 75% of the rats for at least two weeks. [3] Indomethacin (4ppm) reduces tumor yield by 78% in SKH:HR-1-hrBr hairless mice. [4] Indomethacin (20 mg/kg, orally) increases the total area of gastric erosions and concentration of lipid peroxides in the gastric mucosa of rats. Indomethacin increases the alpha-tocopherol:total cholesterol ratio in serum. Indomethacin inhibits the increases in gastric mucosal erosions and lipid peroxides in the gastric mucosa, and the reduction of serum alpha-tocopherol. [5]

Protocol

Solubility (25°C)

In vitro DMSO 72 mg/mL (201.23 mM)
Ethanol 24 mg/mL (67.07 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+30% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.
2mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 357.79
Formula

C19H16ClNO4

CAS No. 53-86-1
Storage powder
in solvent
Synonyms N/A

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04025177 Not yet recruiting Drug: Indomethacin Injection Patent Ductus Arteriosus After Premature Birth University of Manitoba|St. Boniface General Hospital Research Centre|Health Sciences Centre Winnipeg Manitoba|University at Buffalo January 2020 Phase 2

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID