Indomethacin (NSC-77541)

For research use only.

Catalog No.S1723 Synonyms: Indometacin

10 publications

Indomethacin (NSC-77541) Chemical Structure

CAS No. 53-86-1

Indomethacin (NSC-77541, Indometacin) is a nonselective COX1 and COX2 inhibitor with IC50 of 0.1 μg/mL and 5 μg/mL, respectively, used to reduce fever, pain, stiffness, and swelling.

Selleck's Indomethacin (NSC-77541) has been cited by 10 publications

1 Customer Review

  • Indicated PN and MES spheres were shown expressing GFP under the control of a 7 × TCF/LEF optimal promoter cassette (7 × TOP) and constitutively expressed nuclear mCherry. PN 84 and MES 1123 were treated with Wnt3a (200 ng/ml), indomethacin (Indo; 20 μM), or a control (vehicle). Scale bar, 100 μm. Bar graph, quantification of fluorescent signal of GFP (TOP-GFP) versus mCherry (PGK-H2BmCherry). The percentage of GFP- and mCherry-positive spheres was determined by FACS.

    Nat Commun, 2016, 7:12885. Indomethacin (NSC-77541) purchased from Selleck.

Purity & Quality Control

Choose Selective COX Inhibitors

Biological Activity

Description Indomethacin (NSC-77541, Indometacin) is a nonselective COX1 and COX2 inhibitor with IC50 of 0.1 μg/mL and 5 μg/mL, respectively, used to reduce fever, pain, stiffness, and swelling.
Targets
COX1 [6] COX-2 [6]
0.28 μM 14 μM
In vitro

Indomethacin inhibits transcription of a beta-catenin/TCF-responsive reporter gene in a dose dependent manner. Indomethacin also downregulates the beta-catenin/TCF transcriptional target cyclin D1. Indomethacin attenuates the transcription of beta-catenin/TCF-responsive genes, by modulating TCF activity without disrupting beta-catenin/TCF complex formation. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HeLa cells NVHXdop4TnWwY4Tpc44h[XO|YYm= NYPNdYNpOjBibXnudy=> M3;XVGFod26rc4SgZYN1cX[rdImgZZQh[W6mcn;n[Y4hemWlZYD0c5IhMHWwa37ve44hd3KrZ3nuLUBmgHC{ZYPz[YQhcW5iSHXMZUBk\WyuczDjc{1mgHC{ZYPzbY5oKFCVQT2oRXJGMTRvTIXjNVMh[XO|ZYPz[YQh[XNiaX7keYN1cW:wIH;mJGRJXC2rbnT1Z4VlKGy3Y3nm[ZJie2ViYXP0bZZqfHliYX\0[ZIhOjBibXnud{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYmsJGVEPTB;MD6xOEBvVQ>? M{nHUlI{PDN{MEm1
J774 cells M2Xtb2Z2dmO2aX;uJIF{e2G7 NXHYRVR4UW5idnn0do8hcW6qaXLpeI9zgSCjY4Tpeol1gSCjZ3HpcpN1KFC{b4P0ZYdt[W6maX6gS{9JKHO7boToZZNmKDFiaX6gcZVzcW6nIFq3O|Qh[2WubIOsJGlEPTB;MjDuUS=> MoPiNVU5PTdzNEm=
U-937 cells NWj0VXM4TnWwY4Tpc44h[XO|YYm= MYLJckB3cXS{bzDpcohq[mm2b4L5JIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iUILvd5Ri\2yjbnTpckBIN0hic4nueIhie2ViMTCoR29ZNTFrIHnuJHUuQTN5IHPlcIx{NCCLQ{WwQVIhdk1? NILiPXMyOjh5N{W4OC=>
rat synovial cells MlrVSpVv[3Srb36gZZN{[Xl? MmmzTY4hfmm2cn:gbY5pcWKrdH;yfUBm\m[nY4Sgc44heHKxZIXjeIlwdiCxZjDwdo9{fGGpbHHu[IlvKEV{IDjQS2UzMSCrbjDyZZQhe3mwb4\pZYwh[2WubIOsJGlEPTB;Mj65JI5O MYqxNFgzOTdzNh?=
143982 cells NUDJVFNnTnWwY4Tpc44h[XO|YYm= MUPJckB3cXS{bzDpcohq[mm2b4L5JIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iUILvd5Ri\2yjbnTpckBIN0hic4nueIhie2ViMjCoR29ZNTJrIHnuJFE1Ozl6MjDj[YxteyxiSVO1NF06KG6P MnHxNVI5Pzd3OES=
CHO cells M4qwbGZ2dmO2aX;uJIF{e2G7 M3XwNmlvKH[rdILvJIlvcGmkaYTvdpkheG:2ZX7jfUBi\2GrboP0JIh2dWGwIFPPXE0yKGmwIIP0ZYJtgSC2cnHud4Zm[3SnZDDjbIlv\XOnIHjhcZN1\XJib4\hdpkhMEOKTzmgZ4VtdHNuIFnDOVA:OThibl2= NILEXGgyODV5Nk[4OC=>
RBL-1 cell line MkX4SpVv[3Srb36gZZN{[Xl? NVK5OllyUW6qaXLpeIlwdiCxZjDQdo9{fGGpbHHu[IlvKEdxSDDzfY51cGG|ZTDpckBqdnSjY4SgVmJNNTFiY3XscEBtcW6nLDDJR|UxRTBwNTFOwG0> MlzNNlEyPTV6Nh?=
SF-9 insect cells NVnC[VB[TnWwY4Tpc44h[XO|YYm= M2[yclIxKE2rboO= M17JRWlvcGmkaYTpc44hd2ZiaIXtZY4hS0:[MjDlfJBz\XO|ZXSgbY4hW0ZvOTDpcpNm[3RiY3XscJMh[XO|ZYPz[YQh[XNiY3;ueoVze2mxbjDv[kBcOTSFXT3hdoFkcGmmb37pZ{Bi[2mmIITvJHsyPEOfLYDyc5N1[WeuYX7kbY5{KHC{ZXnuZ5Vj[XSnZDDmc5IhOjBibXnud{BjgSC2aHnuMYxigWW{IHPodo9u[XSxZ4LhdIh6KGGwYXz5d4l{NCCLQ{WwQVAvPzVizszN NFLHWJczOTV{NEW4Oy=>
mouse RAW264.7 cells MmrVSpVv[3Srb36gZZN{[Xl? Mo\aNlQhcA>? MX\BcpRqcW6obHHtcYF1d3K7IHHjeIl3cXS7IHnuJI1wfXOnIGLBW|I3PC55IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhVFCVLXnu[JVk\WRiTl:gdJJw\HWldHnvckBi\nSncjCyOEBpenNiYomgS5Jq\XO|IHHzd4F6NCCLQ{WwQVEvOjVizszN NHzHTGkzPDhyNkOxNC=>
mouse BV2 cells NGfFVYpHfW6ldHnvckBie3OjeR?= MXiyOEBp M4LpVmFvfGmrbn\sZY1u[XSxcomgZYN1cX[rdImgbY4hdW:3c3WgRnYzKGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gUHBUNWmwZIXj[YQhVk9icILv[JVkfGmxbjDh[pRmeiB{NDDodpMh[nliR4Lp[ZN{KGG|c3H5MEBKSzVyPUeuNUDPxE1? M37tVFI1ODR{MEC3
human 2008 cells M13KSGZ2dmO2aX;uJIF{e2G7 MV7Jcohq[mm2aX;uJI9nKGi3bXHuJG1TWDFiaX6gbJVu[W5iMkCwPEBk\WyuczygTWM2OD1zMjFOwG0> MlLsNVg4ODd6OES=
A549 cells  NHzhdYtHfW6ldHnvckBie3OjeR?= MojRNVAh|ryP NXTyWYlwUW6qaXLpeIlwdiCxZjDtVGdGWzFiaX6gbJVu[W5iQUW0PUBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIFnMMVEu[mW2YT3pcoR2[2WmIGDHSVIh\m:{bXH0bY9vKGG2IEGwJJVOKGK7IHPlcIwucW62YXP0JIF{e2G7 MV6xPVA2Ozd3MR?=
HepG2 cells M33OZWZ2dmO2aX;uJIF{e2G7 MXixNFAh|ryP MnTNTY5pcWKrdHnvckBw\iCPUmCxMY1m\GmjdHXkJIRwgG:{dXLpZ4lvKGWoZnz1fEBqdiCmb4jvdpVjcWOrbjDy[ZNqe3SjboSgbJVu[W5iSHXwS|Ih[2WubIOgZZQhOTByIIXNJIJ6KG[ub4egZ5l1d22ndIL5 MnfKNlA1PTV3N{i=
THP1 cells Mn;sSpVv[3Srb36gZZN{[Xl? M1;RW|ExOCEQvF2= NFnuUWk{OCCvaX7z MX;JdpJmfmW{c3nicIUhcW6qaXLpeIlwdiCxZjDDU3guOSCrbjDoeY1idiCWSGCxJINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiYYLhZ4hq\G:waXOgZYNq\C2rbnT1Z4VlKFS[QkKg[o9zdWG2aX;uJIF1KDFyMDD1UUBqdmO3YnH0[YQh\m:{IEOwJI1qdnNiZn;scI94\WRiYomgZ49ueG:3bnSge4F{cG:3dDDt[YF{fXKnZDCzNEBucW6|IIDvd5Qh[XKjY3jp[I9vcWNiYXPp[EBkcGGubHXu[4Uh[nlicnHkbY9qdW23bn;hd5NigQ>? MViyN|Y2OTN3OR?=
MDA-MB-231 cells M2fle2Z2dmO2aX;uJIF{e2G7 M{XE[FExOCEQvF2= M3f6eFMxKG2rboO= M3Hi[2lzemW4ZYLzbYJt\SCrbnjpZol1cW:wIH;mJGNQYC1zIHnuJIh2dWGwIF3ERU1OSi1{M{GgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCjcnHjbIlld26rYzDhZ4llNWmwZIXj[YQhWEeHMjDmc5Ju[XSrb36gZZQhOTByIIXNJIlv[3WkYYTl[EBnd3JiM{CgcYlveyCob3zsc5dm\CCkeTDjc41xd3WwZDD3ZZNpd3W2IH3lZZN2emWmIEOwJI1qdnNicH;zeEBiemGlaHnkc45q[yCjY3nkJINp[WyuZX7n[UBjgSC{YXTpc4ludXWwb3Hzd4F6 NYrJVWM5OjN4NUGzOVk>
LNCAP cells MWPGeY5kfGmxbjDhd5NigQ>? NXTWSZhrOzBizszN MmfrOFghcA>? MmrsTY5pcWKrdHnvckBw\iCDS2KxR|MhMHWwa37ve44hd3KrZ3nuLU1u\WSrYYTl[EB1\XO2b4P0[ZJwdmVvMUfi[ZRiNWeudXP1do9vcWSnIH\vdo1ifGmxbjDlfJBz\XO|ZXSgbY4hcHWvYX6gUG5ESVBiY3XscJMh[XRiM{CgeW0h[W[2ZYKgOFghcHK|IHnuJJBz\XOnbnPlJI9nKDRvYX7kdo9{fGWwZT2zMFE4NWSrb37l NILvUIMzOzR|MkC5OS=>
mouse BV2 cells NVHDdZVbS3m2b4TvfIlkcXS7IHHzd4F6 NFS1Z5gzPCCq MmnXR5l1d3SxeHnjbZR6KGGpYXnud5QhdW:3c3WgRnYzKGOnbHzzJIF{e2W|c3XkJIF{KG2jeHnteY0hdm:wLYTvfIlkKGOxbnPlcpRz[XSrb36gZYZ1\XJiMkSgbJJ{KGK7IF3UWEBie3OjeR?= MUmyN|cyOTlzNx?=

... Click to View More Cell Line Experimental Data

In vivo Indomethacin increases BrdU+ cells of all lineages and reduces microglial/monocyte activation in rats after focal cerebral ischemia. [2] Indomethacin (7.5 mg/kg, one injection) produces acute injury and inflammation in the distal jejunum and proximal ileum that are maximal at three days and completely resolves within one week. Indomethacin (two daily subcutaneous injections) produces a more extensive and chronic inflammation that lasts in an active form in more than 75% of the rats for at least two weeks. [3] Indomethacin (4ppm) reduces tumor yield by 78% in SKH:HR-1-hrBr hairless mice. [4] Indomethacin (20 mg/kg, orally) increases the total area of gastric erosions and concentration of lipid peroxides in the gastric mucosa of rats. Indomethacin increases the alpha-tocopherol:total cholesterol ratio in serum. Indomethacin inhibits the increases in gastric mucosal erosions and lipid peroxides in the gastric mucosa, and the reduction of serum alpha-tocopherol. [5]

Protocol

Solubility (25°C)

In vitro DMSO 72 mg/mL (201.23 mM)
Water Insoluble
Ethanol ''24 mg/mL
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+30% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.
2mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 357.79
Formula

C19H16ClNO4

CAS No. 53-86-1
Storage powder
in solvent
Synonyms Indometacin
Smiles CC1=C(C2=C(N1C(=O)C3=CC=C(C=C3)Cl)C=CC(=C2)OC)CC(=O)O

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04821323 Completed Drug: Indomethacin|Diagnostic Test: Lactulose-mannitol Healthy Janssen Research & Development LLC March 10 2021 Early Phase 1
NCT04025177 Not yet recruiting Drug: Indomethacin Injection Patent Ductus Arteriosus After Premature Birth University of Manitoba|St. Boniface Hospital|Health Sciences Centre Winnipeg Manitoba|University at Buffalo January 2020 Phase 2

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COX Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID