Indomethacin

For research use only.

Catalog No.S1723 Synonyms: Indometacin

9 publications

Indomethacin  Chemical Structure

CAS No. 53-86-1

Indomethacin (Indometacin) is a nonselective COX1 and COX2 inhibitor with IC50 of 0.1 μg/mL and 5 μg/mL, respectively, used to reduce fever, pain, stiffness, and swelling.

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10mM (1mL in DMSO) USD 130 In stock
USD 97 In stock
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Selleck's Indomethacin has been cited by 9 publications

1 Customer Review

  • Indicated PN and MES spheres were shown expressing GFP under the control of a 7 × TCF/LEF optimal promoter cassette (7 × TOP) and constitutively expressed nuclear mCherry. PN 84 and MES 1123 were treated with Wnt3a (200 ng/ml), indomethacin (Indo; 20 μM), or a control (vehicle). Scale bar, 100 μm. Bar graph, quantification of fluorescent signal of GFP (TOP-GFP) versus mCherry (PGK-H2BmCherry). The percentage of GFP- and mCherry-positive spheres was determined by FACS.

    Nat Commun, 2016, 7:12885. Indomethacin purchased from Selleck.

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Biological Activity

Description Indomethacin (Indometacin) is a nonselective COX1 and COX2 inhibitor with IC50 of 0.1 μg/mL and 5 μg/mL, respectively, used to reduce fever, pain, stiffness, and swelling.
Targets
COX1 [6] COX-2 [6]
0.28 μM 14 μM
In vitro

Indomethacin inhibits transcription of a beta-catenin/TCF-responsive reporter gene in a dose dependent manner. Indomethacin also downregulates the beta-catenin/TCF transcriptional target cyclin D1. Indomethacin attenuates the transcription of beta-catenin/TCF-responsive genes, by modulating TCF activity without disrupting beta-catenin/TCF complex formation. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HeLa cells MXPGeY5kfGmxbjDhd5NigQ>? M1PBUFIxKG2rboO= MkHERYdwdmm|dDDhZ5Rqfmm2eTDheEBidmS{b3flckBz\WOncITvdkApfW6tbn;3ckBwemmpaX6pJIV5eHKnc4Pl[EBqdiCKZVzhJINmdGy|IHPvMYV5eHKnc4PpcochWFODLTjBVmUqPC2OdXOxN{Bie3Onc4Pl[EBieyCrbnT1Z5Rqd25ib3[gSGhVNWmwZIXj[YQhdHWlaX\ldoF{\SCjY4Tpeol1gSCjZoTldkAzOCCvaX7zJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygSWM2OD1yLkG0JI5O MnPBNlM1OzJyOUW=
J774 cells M1z0cGZ2dmO2aX;uJIF{e2G7 NX\Qe2lCUW5idnn0do8hcW6qaXLpeI9zgSCjY4Tpeol1gSCjZ3HpcpN1KFC{b4P0ZYdt[W6maX6gS{9JKHO7boToZZNmKDFiaX6gcZVzcW6nIFq3O|Qh[2WubIOsJGlEPTB;MjDuUS=> MVOxOVg2PzF2OR?=
U-937 cells NWjiXVlMTnWwY4Tpc44h[XO|YYm= MV;JckB3cXS{bzDpcohq[mm2b4L5JIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iUILvd5Ri\2yjbnTpckBIN0hic4nueIhie2ViMTCoR29ZNTFrIHnuJHUuQTN5IHPlcIx{NCCLQ{WwQVIhdk1? Ml3oNVI5Pzd3OES=
rat synovial cells MWXGeY5kfGmxbjDhd5NigQ>? M1HMfWlvKH[rdILvJIlvcGmkaYTvdpkh\W[oZXP0JI9vKHC{b3T1Z5Rqd25ib3[gdJJwe3SjZ3zhcoRqdiCHMjCoVGdGOiliaX6gdoF1KHO7bn;2bYFtKGOnbHzzMEBKSzVyPUKuPUBvVQ>? M4T3VVExQDJzN{G2
143982 cells M13Ke2Z2dmO2aX;uJIF{e2G7 MkfETY4hfmm2cn:gbY5pcWKrdH;yfUBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGDyc5N1[WeuYX7kbY4hTy:KIIP5cpRp[XOnIEKgLGNQYC1{KTDpckAyPDN7OEKgZ4VtdHNuIFnDOVA:QSCwTR?= NHzDdFEyOjh5N{W4OC=>
CHO cells MlfuSpVv[3Srb36gZZN{[Xl? MY\JckB3cXS{bzDpcohq[mm2b4L5JJBwfGWwY4mgZYdicW6|dDDoeY1idiCFT2itNUBqdiC|dHHicJkhfHKjboPm[YN1\WRiY3jpcoV{\SCqYX3zeIVzKG:4YYL5JEhEUE9rIHPlcIx{NCCLQ{WwQVE5KG6P MnTWNVA2PzZ4OES=
RBL-1 cell line NVf2OXRHTnWwY4Tpc44h[XO|YYm= MUDJcohq[mm2aX;uJI9nKFC{b4P0ZYdt[W6maX6gS{9JKHO7boToZZNmKGmwIHnueIFkfCCUQlytNUBk\WyuIHzpcoUtKEmFNUC9NE42KM7:TR?= MmjoNlEyPTV6Nh?=
SF-9 insect cells M2TjfGZ2dmO2aX;uJIF{e2G7 MU[yNEBOcW6| MmroTY5pcWKrdHnvckBw\iCqdX3hckBEV1h{IHX4dJJme3OnZDDpckBUTi17IHnud4VkfCClZXzsd{Bie3Onc4Pl[EBieyClb372[ZJ{cW:wIH;mJHsyPEOfLXHyZYNpcWSxbnnjJIFkcWRidH:gX|E1S11vcILvd5Ri\2yjbnTpcpMheHKnaX7jeYJifGWmIH\vdkAzOCCvaX7zJIJ6KHSqaX6tcIF6\XJiY3jyc41ifG:pcnHwbJkh[W6jbInzbZMtKEmFNUC9NE44PSEQvF2= NEOzcHUzOTV{NEW4Oy=>
mouse RAW264.7 cells NHjvO5NHfW6ldHnvckBie3OjeR?= NEi3R3czPCCq MkjPRY51cWmwZnzhcY1ifG:{eTDhZ5Rqfmm2eTDpckBud3W|ZTDSRXczPjRwNzDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKEySUz3pcoR2[2WmIF7PJJBzd2S3Y4Tpc44h[W[2ZYKgNlQhcHK|IHL5JGdzcWW|czDhd5NigSxiSVO1NF0yNjJ3IN88US=> NXLKUmt[OjR6ME[zNVA>
mouse BV2 cells M3L6fmZ2dmO2aX;uJIF{e2G7 NXX0dJBpOjRiaB?= NI\5NoZCdnSraX7mcIFudWG2b4L5JIFkfGm4aYT5JIlvKG2xdYPlJGJXOiClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJGxRWy2rbnT1Z4VlKE6RIIDyc4R2[3Srb36gZYZ1\XJiMkSgbJJ{KGK7IFfybYV{eyCjc4PhfUwhUUN3ME23MlEh|ryP M1ruUlI1ODR{MEC3
human 2008 cells M2m2fWZ2dmO2aX;uJIF{e2G7 NX3ubIJOUW6qaXLpeIlwdiCxZjDoeY1idiCPUmCxJIlvKGi3bXHuJFIxODhiY3XscJMtKEmFNUC9NVIh|ryP Mnn5NVg4ODd6OES=
A549 cells  MkTISpVv[3Srb36gZZN{[Xl? NUHrflZCOTBizszN NXHJV|Z6UW6qaXLpeIlwdiCxZjDtVGdGWzFiaX6gbJVu[W5iQUW0PUBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIFnMMVEu[mW2YT3pcoR2[2WmIGDHSVIh\m:{bXH0bY9vKGG2IEGwJJVOKGK7IHPlcIwucW62YXP0JIF{e2G7 MXixPVA2Ozd3MR?=
HepG2 cells NFOyW29HfW6ldHnvckBie3OjeR?= NXPGNIRIOTByIN88US=> NUm4emdRUW6qaXLpeIlwdiCxZjDNVnAyNW2nZHnheIVlKGSxeH;yeYJq[2mwIHXm[ox2gCCrbjDkc5hwenWkaXPpckBz\XOrc4ThcpQhcHWvYX6gTIVxTzJiY3XscJMh[XRiMUCwJJVOKGK7IH\sc5ch[3m2b33leJJ6 MnnUNlA1PTV3N{i=
THP1 cells MWTGeY5kfGmxbjDhd5NigQ>? NGHuSG8yODBizszN NEfycFc{OCCvaX7z M1f1WmlzemW4ZYLzbYJt\SCrbnjpZol1cW:wIH;mJGNQYC1zIHnuJIh2dWGwIGTIVFEh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjDhdoFkcGmmb37pZ{Bi[2mmLXnu[JVk\WRiVGjCNkBnd3KvYYTpc44h[XRiMUCwJJVOKGmwY4XiZZRm\CCob4KgN|AhdWmwczDmc4xtd3enZDDifUBkd22yb4Xu[EB4[XOqb4X0JI1m[XO3cnXkJFMxKG2rboOgdI9{fCCjcnHjbIlld26rYzDhZ4llKGOqYXzs[Y5o\SCkeTDyZYRqd2mvbYXuc4F{e2G7 MnTnNlM3PTF|NUm=
MDA-MB-231 cells NYf4e2h7TnWwY4Tpc44h[XO|YYm= MkfSNVAxKM7:TR?= NEm2W5g{OCCvaX7z M2jrO2lzemW4ZYLzbYJt\SCrbnjpZol1cW:wIH;mJGNQYC1zIHnuJIh2dWGwIF3ERU1OSi1{M{GgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCjcnHjbIlld26rYzDhZ4llNWmwZIXj[YQhWEeHMjDmc5Ju[XSrb36gZZQhOTByIIXNJIlv[3WkYYTl[EBnd3JiM{CgcYlveyCob3zsc5dm\CCkeTDjc41xd3WwZDD3ZZNpd3W2IH3lZZN2emWmIEOwJI1qdnNicH;zeEBiemGlaHnkc45q[yCjY3nkJINp[WyuZX7n[UBjgSC{YXTpc4ludXWwb3Hzd4F6 MWeyN|Y2OTN3OR?=
LNCAP cells MkjpSpVv[3Srb36gZZN{[Xl? MUezNEDPxE1? NEjnUGU1QCCq M3TQTGlvcGmkaYTpc44hd2ZiQVvSNWM{KCi3bnvuc5dvKG:{aXfpckkudWWmaXH0[YQhfGW|dH;zeIVzd26nLUG3ZoV1[S2pbIXjeZJwdmmmZTDmc5Ju[XSrb36g[ZhxemW|c3XkJIlvKGi3bXHuJGxPS0GSIHPlcIx{KGG2IEOwJJVOKGGodHXyJFQ5KGi{czDpckBxemW|ZX7j[UBw\iB2LXHu[JJwe3SnbnWtN{wyPy2maX;u[S=> M{XBR|I{PDN{MEm1
mouse BV2 cells MYHDfZRwfG:6aXPpeJkh[XO|YYm= M4WzfFI1KGh? NY\GTZRvS3m2b4TvfIlkcXS7IHHnZYlve3RibX;1d4UhSlZ{IHPlcIx{KGG|c3Xzd4VlKGG|IH3hfIlufW1ibn;uMZRwgGmlIHPvcoNmdnS{YYTpc44h[W[2ZYKgNlQhcHK|IHL5JG1VXCCjc4PhfS=> NITJUFIzOzdzMUmxOy=>

... Click to View More Cell Line Experimental Data

In vivo Indomethacin increases BrdU+ cells of all lineages and reduces microglial/monocyte activation in rats after focal cerebral ischemia. [2] Indomethacin (7.5 mg/kg, one injection) produces acute injury and inflammation in the distal jejunum and proximal ileum that are maximal at three days and completely resolves within one week. Indomethacin (two daily subcutaneous injections) produces a more extensive and chronic inflammation that lasts in an active form in more than 75% of the rats for at least two weeks. [3] Indomethacin (4ppm) reduces tumor yield by 78% in SKH:HR-1-hrBr hairless mice. [4] Indomethacin (20 mg/kg, orally) increases the total area of gastric erosions and concentration of lipid peroxides in the gastric mucosa of rats. Indomethacin increases the alpha-tocopherol:total cholesterol ratio in serum. Indomethacin inhibits the increases in gastric mucosal erosions and lipid peroxides in the gastric mucosa, and the reduction of serum alpha-tocopherol. [5]

Protocol

Solubility (25°C)

In vitro DMSO 72 mg/mL (201.23 mM)
Water Insoluble
Ethanol '24 mg/mL
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+30% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.
2mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 357.79
Formula

C19H16ClNO4

CAS No. 53-86-1
Storage powder
in solvent
Synonyms Indometacin
Smiles CC1=C(C2=C(N1C(=O)C3=CC=C(C=C3)Cl)C=CC(=C2)OC)CC(=O)O

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04025177 Not yet recruiting Drug: Indomethacin Injection Patent Ductus Arteriosus After Premature Birth University of Manitoba|St. Boniface Hospital|Health Sciences Centre Winnipeg Manitoba|University at Buffalo January 2020 Phase 2

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID