Fludarabine (NSC 118218)

Catalog No.S1491 Synonyms: FaraA, Fludarabinum

For research use only.

Fludarabine (NSC 118218, FaraA, Fludarabinum) is a STAT1 activation inhibitor which causes a specific depletion of STAT1 protein (and mRNA) but not of other STATs. Also a DNA synthesis inhibitor in vascular smooth muscle cells. Fludarabine induces apoptosis.

Fludarabine (NSC 118218) Chemical Structure

CAS No. 21679-14-1

Selleck's Fludarabine (NSC 118218) has been cited by 127 publications

Purity & Quality Control

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Biological Activity

Description Fludarabine (NSC 118218, FaraA, Fludarabinum) is a STAT1 activation inhibitor which causes a specific depletion of STAT1 protein (and mRNA) but not of other STATs. Also a DNA synthesis inhibitor in vascular smooth muscle cells. Fludarabine induces apoptosis.
Targets
STAT1 [4]
(Vascular smooth muscle cells)
In vitro

Fludarabine efficiently inhibits the proliferation of RPMI 8226 cells with IC50 of 1.54 μg/mL. The IC50 of Fludarabine against MM.1S and MM.1R cells is 13.48 μg/mL and 33.79 μg/mL, respectively. In contrast, U266 cells are resistant to Fludarabine with IC50 of 222.2 μg/mL. Fludarabine treatment results in increased number of cells in the G1 phase of cell cycle, accompanied with a concomitant reduction of cells at the S phase of cell cycle in a time-dependent manner. Fludarabine induces a cell cycle block and triggers apoptosis in MM cells. Fludarabine triggers time-dependent cleavage of caspase-8, -9, and -3, -7, followed by PARP cleavage. Fludarabine increases expression of Bax in a time-dependent fashion, while the expression of Bak doesn't change. After exposure to Fludarabine for 12 hours, RPMI 8226 cells shows a loss of membrane potential with 61.05% of the cells expressing low fluorescence of rhodamine 123 compared with 8.62% of cells in untreated control. [1] To enhance solubility, Fludarabine is formulated as the monophosphate (F-ara-AMP, fudarabine), which is instantaneously and quantitatively dephosphorylated to the parent nucleoside upon intravenous infusion. Inside the cells rephosphorylation occurs which leads to fuoroadenine arabinoside triphosphate (F-ara-ATP), the major cytotoxic metabolite of F-ara-A. [2] Fludarabine can also induce pro-inflammatory stimulation of monocytic cells, as evaluated by increased expression of ICAM-1 and IL-8 release. [3] Fludarabine does not affect the growth of ovarian cancer cell lines, whereas it induces marked and dose-dependent inhibition of proliferation in melanoma cell lines. [4] Fludarabine induces significant reduction of STAT-1 phosphorylation, whereas it does not change JAK2 activation. Interestingly, Fludarabine does not significantly affect the phosphorylation of these three STAT proteins. Fludarabine (1.5 mg) significantly prevents STAT-1 phosphorylation and also reduces the increased amount of this protein. No significant changes are demonstrated in JAK2 phosphorylation at 2 days, but Fludarabine inhibits JAK2-increased expression at 7 days. Fludarabine specifically inhibits STAT-1 activation without affecting other STAT proteins and consequently diminishes VSMC proliferation. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Jeko-1  M3O1OGZ2dmO2aX;uJGF{e2G7 M{nHUFIxKM7:TR?= MXWyOEBp NWG4bnV2cW6qaXLpeJMh\XiycnXzd4lwdiCxZjDJSG8> MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTl2MEexNkc,OjV7NEC3NVI9N2F-
MV-4-11 MljaRZBweHSxc3nzJGF{e2G7 NYfpdm9sOi53IN88US=> NGTid481QCCq M{DTcolv\HWlZYOgZZBweHSxc3nzJJNtcWeqdHz5 MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTFzMUW4N{c,OjVzMUG1PFM9N2F-
THP-1 MmPsRZBweHSxc3nzJGF{e2G7 NX3EOII1Oi53IN88US=> M4rYUlQ5KGh? MUDpcoR2[2W|IHHwc5B1d3OrczDzcIlocHSueR?= NVTIXFNYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxNVE2QDNpPkK1NVEyPTh|PD;hQi=>
MOLM 13 Mn7zRZBweHSxc3nzJGF{e2G7 MojRNk42KM7:TR?= M3e2W|Q5KGh? MnjDbY5lfWOnczDhdI9xfG:|aYOgd4xq\2i2bIm= MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTFzMUW4N{c,OjVzMUG1PFM9N2F-
KBM3/Bu2506 MUTBdI9xfG:|aYOgRZN{[Xl? MVKyMlUh|ryP NYjRN3JRPDhiaB?= NI\nZoRqdmS3Y3XzJIFxd3C2b4Ppd{B{dGmpaITsfS=> NV;DWmU{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxNVE2QDNpPkK1NVEyPTh|PD;hQi=>
Nalm-6 M{\5b2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4LTNGlEPTB;MUig{txO MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTB4MUGwNUc,OjVyNkGxNFE9N2F-
Reh NWjtUppVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1TqcGlEPTB;M{Cg{txO NGLvOYg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUC2NVExOSd-MkWwOlEyODF:L3G+
U2937 NFH4fIFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFHxfWpKSzVyPUG2JO69VQ>? NFjuXGg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUC2NVExOSd-MkWwOlEyODF:L3G+
Mec-1 MoHyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4rDbWlEPTExvK61NFAh|ryP NELWOW09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUC2NVExOSd-MkWwOlEyODF:L3G+
RPMI-8226 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUnHSYVtUUN3ME21NFAh|ryP MoDqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyNkGxNFEoRjJ3ME[xNVAyRC:jPh?=
Molt-4 MkjxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUfMXoViUUN3ME2xPFAh|ryP NVzh[lNURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWwOlEyODFpPkK1NFYyOTBzPD;hQi=>
Nalm-6-FluR NVLMPWwyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXXJR|UxRTJ3MDFOwG0> MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTB4MUGwNUc,OjVyNkGxNFE9N2F-
Raji  M{HtRmZ2dmO2aX;uJGF{e2G7 MlTVN:Kh|ryP MUWyOE81QC95MjDo NVu3bmZRcW6mdXPld{Bi[2O3bYXsZZRqd26|IH;mJJA2OyxicE[zJIFv\CCyN{RCpC=> NVLNdJNKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5OFA3QTVpPkK0PVQxPjl3PD;hQi=>
PBMC NYX0cGgyTnWwY4Tpc44hSXO|YYm= NF31OGM2OC9zMECg{txO M1Xid|I1KGh? M4raXGROW09? MV;pcohq[mm2czDTWGFVOSCyaH;zdIhwenmuYYTpc44> NHj6Zlc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmxNVg4Oid-MkS5NVE5PzJ:L3G+
MDA-231 MXjGeY5kfGmxbjDBd5NigQ>? MnrnNVAxKM7:TR?= M3\kVFI1KGh? MV\EUXNQ NVjPXFlb\GWlcnXhd4V{KEmGTzDlfJBz\XO|aX;u MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDlzMUi3Nkc,OjR7MUG4O|I9N2F-
624.38mel  NXPtNmN4TnWwY4Tpc44hSXO|YYm= NYi3VmxUPTBizszN NGHBOXIzPCCq NFTqW5FFVVOR NXT6b2Zi\GWlcnXhd4V{KEmGTzDlfJBz\XO|aX;u M2jYRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUGxPFczLz5{NEmxNVg4OjxxYU6=
MDA-231 NEXWRnBHfW6ldHnvckBCe3OjeR?= MmXROVAuOjByIN88US=> MXmyOEBp MXrEUXNQ M2XmOIlvcGmkaYTzJGlFVyCjY4Tpeol1gSCrbnTldIVv\GWwdHz5JI9nKG2UTlGgcIV3\Wy| MnrFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7MUG4O|IoRjJ2OUGxPFczRC:jPh?=
624.38mel  MV3GeY5kfGmxbjDBd5NigQ>? M4\X[FUxNTJyMDFOwG0> MnzNNlQhcA>? M3L2cWROW09? MYHpcohq[mm2czDJSG8h[WO2aY\peJkhcW6mZYDlcoRmdnSueTDv[kBuWk6DIHzleoVtew>? MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDlzMUi3Nkc,OjR7MUG4O|I9N2F-
HMECs M4LIdWZ2dmO2aX;uJGF{e2G7 M4\hRVExOMLizszNxsA> NGjrZ4k{PsLiaB?= M3HMdolvcGmkaYTzJGlHVs7|wrDhcoQhVFCVIHnu[JVk\WRiU2TBWFEheGixc4Doc5J6dGG2aX;uJIFv\CCLUl[xJIV5eHKnc4Ppc44> NY\BNo9xRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyNVE{OjdpPkK0NlEyOzJ5PD;hQi=>
HMECs  MUfGeY5kfGmxbjDBd5NigQ>? M2TmRVExOMLizszNxsA> MUWzOuKhcA>? MknBbY5pcWKrdIOgTWZP|rIEoH3l[IlifGWmIIDoc5NxcG:{eXzheIlwdiCxZjDTWGFVOSCjbnSgV3RCXDNuIHL1eEBvd3Rib3[gV3RCXDJ? NGnrNpc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEKxNVMzPyd-MkSyNVE{Ojd:L3G+
BJAB MornRZBweHSxc3nzJGF{e2G7 NI\JZ5g2yqEQvF2= NF;G[oUzPCCq MXXpcoR2[2W|IHPlcIwh[XCxcITvd4l{ MlXKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyNUexOFcoRjJ2MEW3NVQ4RC:jPh?=
I-83 MWLBdI9xfG:|aYOgRZN{[Xl? NVqzOJZJPcLizszN NXTv[ng{OjRiaB?= NVfObWxYcW6mdXPld{Bk\WyuIHHwc5B1d3Orcx?= NVTxN5ljRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwOVcyPDdpPkK0NFU4OTR5PD;hQi=>
NALM6 Ml3tRZBweHSxc3nzJGF{e2G7 Mny3OeKh|ryP NUm0c3BIOjRiaB?= NETQNnNqdmS3Y3XzJINmdGxiYYDvdJRwe2m| M13ieFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEW3NVQ4Lz5{NEC1O|E1PzxxYU6=
DU-145 MmD0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnvjNE0yOCEQvHevcYw> MUO0PEBpyqB? M17lS4lvcGmkaYTzJINmdGxiZ4Lve5RpKGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd|NEixOUc,OjN5M{S4NVU9N2F-
Nalm-6 MUPGeY5kfGmxbjDBd5NigQ>? Mn7PNVDDqM7:TR?= MUSxM|IwPCCq Mnj6bY5lfWOnczDheZRweGijZ4m= NEnjR4s9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{[4NVIzOyd-MkO2PFEzOjN:L3G+
Reh MVfGeY5kfGmxbjDBd5NigQ>? MUmxNOKh|ryP MX:xM|IwPCCq NUHOfpB2cW6mdXPld{BifXSxcHjh[5k> M1;5clxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NkixNlI{Lz5{M{[4NVIzOzxxYU6=
Nalm-6 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4jWU2lEPTBi4pk8NVDjiIoQvF2= MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzZ6MUKyN{c,OjN4OEGyNlM9N2F-
Reh M1fvSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVPF[mFLUUN3MDFijNwyOOLCid88US=> NGXxZVA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{[4NVIzOyd-MkO2PFEzOjN:L3G+
HEC1A NWDx[2RWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIXtZlkyODBvNUCwJO69VQ>? M2\iSVI1KGh? MYPpcohq[mm2czDj[YxtKGe{b4f0bEBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> M3LHO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUm1Olk4Lz5{M{W5OVY6PzxxYU6=
AN3CA NGLkRVFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV2xNFAuPTByIN88US=> M4XOZVI1KGh? M13jcYlvcGmkaYTzJINmdGxiZ4Lve5RpKGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz NXrufHlsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1PVU3QTdpPkKzOVk2Pjl5PD;hQi=>
HEC50B M4LRR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnG2NVAxNTVyMDFOwG0> MkTyNlQhcA>? NYn5WXY6cW6qaXLpeJMh[2WubDDndo94fGhic3zp[4h1dHl? NYDiUpBMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1PVU3QTdpPkKzOVk2Pjl5PD;hQi=>
HEC1A MWnBdI9xfG:|aYOgRZN{[Xl? MWmyNE8yODBizszN NX;lTWNtOjRiaB?= M4raTolv\HWlZYOgZZBweHSxc3nzJIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy Mo\WQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3OUW2PVcoRjJ|NUm1Olk4RC:jPh?=
AN3CA MXjBdI9xfG:|aYOgRZN{[Xl? NYHCe4loOjBxMUCwJO69VQ>? MnzONlQhcA>? NXW2eVhqcW6mdXPld{BieG:ydH;zbZMhcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzV7NU[5O{c,OjN3OUW2PVc9N2F-
HEC50B NFfrcnBCeG:ydH;zbZMhSXO|YYm= MWOyNE8yODBizszN M3S5Z|I1KGh? NFPCS3BqdmS3Y3XzJIFxd3C2b4Ppd{B{dGmpaITsfS=> NFHmbVY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{W5OVY6Pyd-MkO1PVU3QTd:L3G+
EHEB MlL6RZBweHSxc3nzJGF{e2G7 NIL4WmE1OCEQvF2= NGLodogzPCCq MmrvbY5lfWOnczDhdI9xfG:|aYO= MmXGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2OUewO|UoRjJ|NEm3NFc2RC:jPh?=
A549 NV7pV4ZvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUf4bWZoUUN3ME2xOU44yrF{LkigxtVO MnPuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN|N{exPVIoRjJ|M{e3NVkzRC:jPh?=
A549 GAPDH-deficient NVW5cIF3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4KzNWlEPTB;MUiuOeKyOi5|INM1US=> MmD1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN|N{exPVIoRjJ|M{e3NVkzRC:jPh?=
CLL  M4LvN2Fxd3C2b4Ppd{BCe3OjeR?= NEPORnAyOCEQvF5CpC=> NGC5RmgzPC17NjDo MnfxbY5lfWOnczDhdI9xfG:2aXOgZ4VtdCCmZXH0bC=> MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjJyN{[4Okc,OjJ{MEe2PFY9N2F-
MEC1 NWnObWhvSXCxcITvd4l{KEG|c3H5 NIrKe4MyODEEoN88US=> MXO3NkBp NVT0V4tscW6mdXPld{BieG:ydH;zbZMhe2mpbnnmbYNidnSueR?= NEf2b4E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkGzNlk4Oyd-MkKxN|I6PzN:L3G+
U937  MVHBdI9xfG:|aYOgRZN{[Xl? MWmwMlgh|ryP NGK3eZU1NTR6IHi= NYHnT404cW6mdXPld{BieG:ydH;zbZMhe2yrZ3j0cJk> MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjB5NEewNEc,OjJyN{S3NFA9N2F-
U937  M1WwN2Fxd3C2b4Ppd{BCe3OjeR?= NYLBb2Y{OSEQvF2= Mlz5PVYhcA>? MUjpcoR2[2W|IHHwc5B1d3OrczDzcIlocHSueR?= Ml7VQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJyMkO1NlMoRjJ{MEKzOVI{RC:jPh?=
Daudi MUXBdI9xfG:|aYOgRZN{[Xl? MWCyNEDPxE1? MnfIPVYhcA>? NYK1XFd6cW6mdXPld{BieG:ydH;zbZMhe2yrZ3j0cJk> MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjB{M{WyN{c,OjJyMkO1NlM9N2F-
J45.01 M4TDOWFxd3C2b4Ppd{BCe3OjeR?= NV3zfGpvOSEQvF2= M2jP[lk3KGh? M3;mWYlv\HWlZYOgZZBweHSxc3nzJJNtcWeqdHz5 MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjB{M{WyN{c,OjJyMkO1NlM9N2F-
RPMI 8226 MmHpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkTsTWM2OD1{NT65xsDDucLiMz63JO69VQ>? MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTl2OEK2OEc,OjF7NEiyOlQ9N2F-
CEM M{HIWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M37JWWlEPTB;Mj60xsDDucLiMD60JO69VQ>? Mlm1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF7NEiyOlQoRjJzOUS4NlY1RC:jPh?=
Raji M13jV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIPZcoZKSzVyPUCuOFfDqMLzwrCwMlA1KM7:TR?= MnOwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF7NEiyOlQoRjJzOUS4NlY1RC:jPh?=
U937 MmLOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4PLN2lEPTB;MD6yOOKhyrIEoECuNFQh|ryP MoXRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF7NEiyOlQoRjJzOUS4NlY1RC:jPh?=
K562 NF3RbXlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{Tl[WlEPTB;MD60OOKhyrIEoECuNFUh|ryP M3XWPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOUS4NlY1Lz5{MUm0PFI3PDxxYU6=
NALM-6 MWXBdI9xfG:|aYOgRZN{[Xl? NWD5RXRKOTBizszNxsA> NIW5[mQzPCCq NGXaNFNqdmS3Y3XzJINmdGxiYYDvdJRwe2m|IIPsbYdpfGy7 MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTZ7OUO4N{c,OjF4OUmzPFM9N2F-
JMV-3 MXvBdI9xfG:|aYOgRZN{[Xl? M2D2dVExKM7:TdMg M1\ye|I1KGh? MlvVbY5lfWOnczDj[YxtKGGyb4D0c5NqeyC|bHnnbJRtgQ>? NXmzOoNyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG2PVk{QDNpPkKxOlk6Ozh|PD;hQi=>
EHEB NFnTU2hHfW6ldHnvckBCe3OjeR?= NIXWdoY2NTVyIN88US=> NX\oW4puOjRiaB?= MVHk[YNz\WG|ZYOgdFIyKGW6cILld5Nqd25ic3nncolncWOjboTsfS=> M130bFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMU[4N|kyLz5{MUG2PFM6OTxxYU6=
JVM-2  MlfRSpVv[3Srb36gRZN{[Xl? M3f5WFMxKM7:TR?= NH;zVXQzPCCq M4PmU4Rm[3KnYYPld{BxOjFiZYjwdoV{e2mxbh?= NWDBZ5drRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGxOlg{QTFpPkKxNVY5OzlzPD;hQi=>
KBM3/Bu2506 MlLwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF;NdVdKSzJyPUCuOlchyrWP Mkm1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB7M{O1NFkoRjJyOUOzOVA6RC:jPh?=
KBM3/Bu2506 NIPRXnpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX:wMlYh|ryP NIDldXMzPCCq NVm3fWs6cW6lcnXhd4V{KHSqZTDj[YxtKG[{YXP0bY9vKGmwIGOtdIhie2V? MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODl|M{WwPUc,OjB7M{O1NFk9N2F-
MDA-MB-231 Mn[4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV7JR|UxRTRwMDFOwG0> MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODR2N{O5NEc,OjB2NEezPVA9N2F-
MCF-7 M3Pxcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NETkemVKSzVyPUG1MlAh|ryP NInCZW49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MES0O|M6OCd-MkC0OFc{QTB:L3G+
HLE-B3  Mnu0SpVv[3Srb36gRZN{[Xl? MWOyOUDPxE1? MlvQOFghcA>? NIDSXVdjdG:la4OgTWZPNc7|4pETbY5lfWOnZDDTWGFVOSCyaH;zdIhwenmuYYTpc44h[W6mIFnEU{BmgHC{ZYPzbY9v MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODR|NUG1PEc,OjB2M{WxOVg9N2F-
K562 NXvobI5JT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4\qcVczKGh? Mmj6TWM2OD1|LkOgcm0> MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODNyN{G5PEc,OjB|MEexPVg9N2F-
BW-225 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXTJR|IxRTFwM{egx7cyOOLKkklCpO69VcLi MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDZ4MUO4NEc,OTh4NkGzPFA9N2F-
OH-65 NI\zenVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV62cZllUUN{ME2xMlM4KMPZMUFijLI5yqEQvF5CpC=> MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDZ4MUO4NEc,OTh4NkGzPFA9N2F-
GR-145 NYftXG5ZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFP6OphKSzJyPUKuO|Qhy5diMUFijLI5KCEQvF5CpC=> MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDZ4MUO4NEc,OTh4NkGzPFA9N2F-
A549 M1:zV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGPxZZdKSzJyPUWuOFghy5diMUFijLI5KM7:TdMg NIrhVW49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOE[2NVM5OCd-MUi2OlE{QDB:L3G+
CaSki  M4PLc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4DFemlEOjB;MT6zO{DEnyBzMPMIllch|ryPwrC= MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDZ4MUO4NEc,OTh4NkGzPFA9N2F-
ZMK-1 NVnjS2t[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M17lemlEOjB;MT6zO{DEnyBzMPMIllYh|ryPwrC= MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDZ4MUO4NEc,OTh4NkGzPFA9N2F-
SKW6.4 MoP6RZBweHSxc3nzJGF{e2G7 MXSwMlAyNTFyIN88US=> NEP1b2UzPC92ODDo NX;FTodrcW6mdXPld{Bk\WyuIHTlZZRpKGmwIHLveIghfGmvZT2gZY5lKGSxc3WtJIRmeGWwZHXueEBu[W6wZYK= NYf6[IV{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUiwPVI{PDBpPkG4NFkzOzRyPD;hQi=>
RPMI 8226 NVu4VWNPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHzWfpczPCCq MXzJR|UxRTFwNUVCpO69VQ>? NGXnZ3k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{m3OlE5Pid-MUe5O|YyQDZ:L3G+
MM.1S MkPiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXK0PEBp M2rOWGlEPTB;MUOuOFjDqM7:TR?= M33TRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5OUe2NVg3Lz5zN{m3OlE5PjxxYU6=
MM.1R M3ryemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVW0PEBp NEDkdoVKSzVyPUOzMlc6KM7:TR?= M3XzblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5OUe2NVg3Lz5zN{m3OlE5PjxxYU6=
U937 NGPpVJlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV3JR|UxRTNuMkCwJOKyKDV4MDDuUS=> Mlr6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV7M{CzOlEoRjF3OUOwN|YyRC:jPh?=
CLL5 MWnBcpRqfHWvb4KgZZN{[Xl? MVK0PEBpenN? MoDvRY51cXS3bX;yJIFkfGm4aYT5JIFo[Wmwc4SgR2xNPSClZXzsd{Bqe2:uYYTl[EBnem:vIFPMUEBx[XSrZX70JIF{e2W|c3XkJIF{KGOnbHygeoli[mmuaYT5JIFnfGW{IES4JIhzeyCkeTDGRWNUKGGwYXz5d4l{NCCHQ{WwJF0hOC5zNjFOwG0v NYTPR2hiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2O|M4OzlpPkK0Olc{PzN7PD;hQi=>
K562 NIjNSYhEgXSxdH;4bYNqfHliYYPzZZk> NIrXTo44OiCqcoO= MmXRR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gdIFkdGm2YYjlcE1z\XOrc4ThcpQhUzV4MjDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOC5{NjFOwG0v NGHXd|Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{ME[wOVY2Pid-MkC2NFU3PTZ:L3G+
CLL3 NYPTS|hlSW62aYT1cY9zKGG|c3H5 MmC2OFghcHK| NXrhXFV3SW62aYT1cY9zKGGldHn2bZR6KGGpYXnud5QhS0yOMzDj[YxteyCrc3;sZZRm\CCocn;tJGNNVCCyYYTp[Y51KGG|c3Xzd4VlKGG|IHPlcIwhfmmjYnnsbZR6KGGodHXyJFQ5KGi{czDifUBHSUOVIHHuZYx6e2m|LDDFR|UxKD1iMD6zOUDPxE1w NG[2WJM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NE[3N|c{QSd-MkS2O|M4Ozl:L3G+
CLL4 M3r0VWFvfGm2dX3vdkBie3OjeR?= MXW0PEBpenN? MmezRY51cXS3bX;yJIFkfGm4aYT5JIFo[Wmwc4SgR2xNPCClZXzsd{Bqe2:uYYTl[EBnem:vIFPMUEBx[XSrZX70JIF{e2W|c3XkJIF{KGOnbHygeoli[mmuaYT5JIFnfGW{IES4JIhzeyCkeTDGRWNUKGGwYXz5d4l{NCCHQ{WwJF0hOC54NDFOwG0v NHvDPXE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NE[3N|c{QSd-MkS2O|M4Ozl:L3G+
CEM-DNR-B M4O5NmN6fG:2b4jpZ4l1gSCjc4PhfS=> NW\4eJM{PzJiaILz MnnBR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gR2VONUSQUj3CJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSBzLkCxJO69VS5? MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODZyNU[1Okc,OjB4MEW2OVY9N2F-
primary CLL M1jjcmN6fG:2b4jpZ4l1gSCjc4PhfS=> MYfDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDwdolu[XK7IFPMUEBk\WyuczygUGQ2OCB;IEGuNUDPxE1w M{[5TlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MUS4N|kzLz5{NUG0PFM6OjxxYU6=
CLL6 NH[0dnlCdnSrdIXtc5Ih[XO|YYm= MoTzOFghcHK| NV;EW29lSW62aYT1cY9zKGGldHn2bZR6KGGpYXnud5QhS0yONjDj[YxteyCrc3;sZZRm\CCocn;tJGNNVCCyYYTp[Y51KGG|c3Xzd4VlKGG|IHPlcIwhfmmjYnnsbZR6KGGodHXyJFQ5KGi{czDifUBHSUOVIHHuZYx6e2m|LDDFR|UxKD1iMT62JO69VS5? M4POXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NkezO|M6Lz5{NE[3N|c{QTxxYU6=
CLL2 NHG1R5VCdnSrdIXtc5Ih[XO|YYm= M3;aNVQ5KGi{cx?= NXLuWGY6SW62aYT1cY9zKGGldHn2bZR6KGGpYXnud5QhS0yOMjDj[YxteyCrc3;sZZRm\CCocn;tJGNNVCCyYYTp[Y51KGG|c3Xzd4VlKGG|IHPlcIwhfmmjYnnsbZR6KGGodHXyJFQ5KGi{czDifUBHSUOVIHHuZYx6e2m|LDDFR|UxKD1iMj62OkDPxE1w MoDIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4N{O3N|koRjJ2NkezO|M6RC:jPh?=
HCT116 NIfnc4lEgXSxdH;4bYNqfHliYYPzZZk> NGXXWXQ4OiCqcoO= NVHCSFROS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUEOWMUG2JINmdGy|IHHmeIVzKDd{IHjyd{BjgSCVUlKgZZN{[XluIFnDOVAhRSB4Lk[g{txONg>? MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR4MkK3O{c,OjV2NkKyO|c9N2F-
PBMC M{HrOmN6fG:2b4jpZ4l1gSCjc4PhfS=> MWe0PEBpenN? NHT4bJVEgXSxdH;4bYNqfHliYXfhbY5{fCCyYYTp[Y51KFCETVOgZYZ1\XJiNEigbJJ{KGK7IFPlcIxVcXS{ZT3CcJVmKGG|c3H5JIlvKHC{ZYPlcoMhd2ZibX;1d4UhVTJzMFK0JINmdGy|LDDJR|UxKD1iMUCg{txONg>? NH7pZWk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUW2NlQyPyd-MkW1OlI1OTd:L3G+
CHO NH[xNlZHfW6ldHnvckBie3OjeR?= MVrCbY5lcW6pIHHm[olvcXS7IHTleIVzdWmwZXSgZpkh\Gm|cHzhZ4Vu\W62IH;mJJNx\WOrZnnjJIJqdmSrbnegc4YhYzF{NVndUk0pPC2jbXnuc{0{NWmxZH;wbIVv\XSqeXypMYFl\W6xc3nu[UBqdiCvZX3idoFv\XNib3[gR2hQKGOnbHzzJJN1[WKueTD0doFve2[nY4Tl[EB4cXSqIITo[UBz[XRiYXTlco9{cW6nIFGzJJJm[2WydH;yMEBMcSB;IEGwMlQh|ryPLh?= NUn0[llsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxN{ewO|MzOCd-N{ewO|MzODxxYU6=
JVM2 MY\BcpRqfHWvb4KgZZN{[Xl? NF;XXJhCdnSrdIXtc5Ih[WO2aY\peJkh[WejaX7zeEBpfW2jbjDKWm0zKGOnbHzzJIF{e2W|c3XkJIF{KGOnbHygeoli[mmuaYT5JIFnfGW{IES4JIhzeyCkeTDGRWNUKGGwYXz5d4l{NCCHQ{WwJF0hOTBwNDFOwG0v M3fVWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NkezO|M6Lz5{NE[3N|c{QTxxYU6=
HeLa Mm\QRY51cXS3bX;yJIF{e2G7 MVrBcpRqfHWvb4KgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKZVzhJINmdGy|IHHzd4V{e2WmIHHzJINmdGxidnnhZoltcXS7IHL5JG1VXCCjc4PhfUwhTUN3MDC9JFE3KM7:TT6= MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ5M{ezPUc,OjR4N{O3N|k9N2F-
CLL1 NVnvem9jSW62aYT1cY9zKGG|c3H5 MWK0PEBpenN? MmfBRY51cXS3bX;yJIFkfGm4aYT5JIFo[Wmwc4SgR2xNOSClZXzsd{Bqe2:uYYTl[EBnem:vIFPMUEBx[XSrZX70JIF{e2W|c3XkJIF{KGOnbHygeoli[mmuaYT5JIFnfGW{IES4JIhzeyCkeTDGRWNUKGGwYXz5d4l{NCCHQ{WwJF0hOTdwMTFOwG0v MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ5M{ezPUc,OjR4N{O3N|k9N2F-
CEM MYDDfZRwfG:6aXPpeJkh[XO|YYm= MVO3NkBpenN? NFS0TmREgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBETU1iY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFE6NjR7IN88UU4> NX3IeVdMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC2NFU3PTZpPkKwOlA2PjV4PD;hQi=>
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NB1643 NVz0fpdZeUiWUzDhd5NigQ>? NULTXm8zeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhVkJzNkSzJINmdGy| NYroUnRkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
SK-N-MC NWDydW8yeUiWUzDhd5NigQ>? M{XaXJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJHNMNU5vTVOgZ4VtdHN? MoDpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
SJ-GBM2 MmLKdWhVWyCjc4PhfS=> NFXqbGlyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBUUi2JQl2yJINmdGy| NWHJbohDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
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Assay
Methods Test Index PMID
Western blot procaspase-9 / procaspase-3 ; p-p53 / p53 ; STAT1 27223263 26677135
Immunofluorescence α-SMA / Vimentin 28322315
Growth inhibition assay Cell viability 24956101
In vivo Tumors treated with PBS grow rapidly to approx-imately 10-fold their initial volume in 25 day, whereas, the tumors in the Fludarabine at 40 mg/kg increase less than 5-fold. A significant antitumor effect of 40 mg/kg Fludarabine on RPMI8226 tumor growth is demonstrated. RPMI8226 tumors treated with 40 mg/kg Fludarabine at day 10 increase apoptotic nuclei. Fludarabine is effective in suppressing RPMI8226 myeloma xenografts in SCID mice. [1]

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: Dexamethasone-sensitive (MM.1S) and -resistant (MM.1R) human MM cell lines, RPMI8226 and U266 cell lines
  • Concentrations: 2 μg/mL
  • Incubation Time: 24 hours
  • Method:

    After treated with Fludarabine or control, dexamethasone-sensitive (MM.1S) and -resistant (MM.1R) human MM cell lines, RPMI8226 and U266 cell lines (5 × 105 cells) are washed twice in phosphate-buffered saline (PBS) and fixed with 70% ice-cold ethanol, then centrifuged and suspended in PBS containing 100 μg/mL RNase A. After incubated for 30 minutes at 37 ºC, samples are resuspended in 25 μg/mL propidium iodide. Flow cytometry is performed on a FACSCalibur automated system. Apoptosis is determined by Annexin V-FITC apoptosis detection kit, according to the manufacturer's instructions. For TUNEL (terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling) assay, cells are analyzed by flow cytometry using the in situ cell death detection kit.

Animal Research:

[1]

  • Animal Models: Severe combined immunodeficient (SCID) mice bearing RPMI 8226 cells
  • Dosages: 40 mg/kg
  • Administration: Administered via i.p.

Solubility (25°C)

In vitro

DMSO 57 mg/mL
(199.83 mM)
Water Insoluble
Ethanol Insoluble

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
5%DMSO+40%PEG300+5%Tween80+50%ddH2O
For best results, use promptly after mixing.

8 mg/mL

Chemical Information

Molecular Weight 285.23
Formula

C10H12FN5O4

CAS No. 21679-14-1
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1=NC2=C(N=C(N=C2N1C3C(C(C(O3)CO)O)O)F)N

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03832127 Not yet recruiting Drug: 18F-Fludarabine Myeloma Nantes University Hospital|Cyceron April 1 2022 Phase 1
NCT04526795 Recruiting Drug: Cytarabine|Drug: Fludarabine|Drug: Pegcrisantaspase Blast Phase Chronic Myelogenous Leukemia BCR-ABL1 Positive|Recurrent Acute Biphenotypic Leukemia|Recurrent Acute Lymphoblastic Leukemia|Recurrent Acute Myeloid Leukemia|Recurrent Chronic Myelogenous Leukemia BCR-ABL1 Positive|Recurrent T-Cell Prolymphocytic Leukemia|Refractory Acute Biphenotypic Leukemia|Refractory Acute Lymphoblastic Leukemia|Refractory Acute Myeloid Leukemia|Refractory Chronic Myelogenous Leukemia BCR-ABL1 Positive|Refractory T-Cell Prolymphocytic Leukemia M.D. Anderson Cancer Center|National Cancer Institute (NCI) April 9 2021 Phase 1

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

Question 1:
how to re-suspend and deliver the inhibitor for in vivo experiments?

Answer:
For S1491, Fludarabine, we tested a vehicle: 30% Propylene glycol, 5% Tween 80, 65% D5W that you can resuspend the compound in at up to 30mg/ml. It's a suspension and can only be given via oral gavage.

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