Docetaxel

For research use only. Not for use in humans.

Catalog No.S1148 Synonyms: RP56976, NSC 628503

56 publications

Docetaxel Chemical Structure

Molecular Weight(MW): 807.88

Docetaxel, an analog of paclitaxel, is an inhibitor of depolymerisation of microtubules by binding to stabilized microtubules.

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10mM (1mL in DMSO) USD 91 In stock
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Selleck's Docetaxel has been cited by 56 publications

Purity & Quality Control

Choose Selective Microtubule Associated Inhibitors

Biological Activity

Description Docetaxel, an analog of paclitaxel, is an inhibitor of depolymerisation of microtubules by binding to stabilized microtubules.
Targets
Microtubules [1]
(Cell-free assay)
In vitro

Docetaxel is a cytotoxic agent, especially for proliferating cells, which is related to its ability to promote the formation of microtubule bundles and induce sustained mitotic arrest, followed by apoptosis of mitotically arrested cells or permanent mitotic block. Docetaxel suppresses microtubule dynamic instability as well as tread-milling, resulting in the failure of chromosomes to segregate to the daughter cells, which in turn triggers premature exit from mitosis rather than a block at this phase of the cell cycle. [2] Docetaxel inhibits the clonogenic survival of Human cancer cell Hs746T (stomach), AGS (stomach), HeLa (cervix), CaSki (cervix), BxPC3 (pancreas), Capan-1 (pancreas) with IC50 of 1 nM, 1 nM, 0.3 nM, 0.3 nM, 0.3 nM and 0.3 nM respectively. [4] Docetaxel inhibits endothelial cell migration that does not affects microtubule gross morphology or inhibit cell proliferation, although they does produce more subtle effects on microtubule dynamics. Docetaxel inhibits HUVEC migration with an observed IC50 of 1 pM. HUVEC chemotaxis stimulated by either of two angiogenic factors, thymidine phosphorylase or VEGF, is inhibited by Docetaxel with IC 50 of 10 pM and is ablated at 1 nM. [7] Docetaxel induces human monocytes, but not RAW 264.7 murine macrophages, Prostaglandin H Synthase-2m (PGHS-2) expression. [8]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HSC-3 NWDEPZhtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH2xTHpKSzVyPUCuNFAxOTN{IN88US=> MYHTRW5ITVJ?
5637 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXL0PYpLUUN3ME2wMlAxODF2MzFOwG0> MmrnV2FPT0WU
HT-144 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFHQTY1KSzVyPUCuNFAxOTV5IN88US=> NGfIXHpUSU6JRWK=
NCI-H292 NWPVUWUzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX7JR|UxRTBwMECwNVU6KM7:TR?= NV3GOHlyW0GQR1XS
HCE-T MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmnCTWM2OD1yLkCwNFE3OiEQvF2= MnLUV2FPT0WU
ESS-1 M3Lxbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmDuTWM2OD1yLkCwNFE4QSEQvF2= M2j6eXNCVkeHUh?=
D-566MG M{XKWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkDKTWM2OD1yLkCwNFE5OiEQvF2= MUXTRW5ITVJ?
CAL-62 NVXJVW9iT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWDJR|UxRTBwMECwNVg2KM7:TR?= MkDNV2FPT0WU
GT3TKB M1XWcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEnCfIRKSzVyPUCuNFAxOjN{IN88US=> MUjTRW5ITVJ?
HSC-4 M1PX[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmHrTWM2OD1yLkCwNFI{QSEQvF2= NFXOSGlUSU6JRWK=
KOSC-2 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYnJR|UxRTBwMECwNlU2KM7:TR?= MWrTRW5ITVJ?
NB14 M3LCb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVTTNZA6UUN3ME2wMlAxODJ4IN88US=> M3HLfnNCVkeHUh?=
ES8 Mlz1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXLJR|UxRTBwMECwNlYh|ryP NY[2[3JHW0GQR1XS
BFTC-905 NUC5UotJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHzNVXZKSzVyPUCuNFAxOjZ6IN88US=> MXTTRW5ITVJ?
A498 NWPHSm1ET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkK0TWM2OD1yLkCwNFI4QCEQvF2= MVLTRW5ITVJ?
HO-1-N-1 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmGyTWM2OD1yLkCwNFI5OSEQvF2= MVPTRW5ITVJ?
SF539 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWjJR|UxRTBwMECwN|AzKM7:TR?= M1\yTXNCVkeHUh?=
HuO-3N1 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVLJR|UxRTBwMECwN|AzKM7:TR?= NIDWV21USU6JRWK=
NCI-H510A NYHIOXptT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnLVTWM2OD1yLkCwNFM{PiEQvF2= NWnKcY55W0GQR1XS
CAL-27 NVPwdZNrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIjvXIJKSzVyPUCuNFAxOzN7IN88US=> MUDTRW5ITVJ?
TE-8 NUmzb|FlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWjJR|UxRTBwMECwN|Y3KM7:TR?= Mn;jV2FPT0WU
VA-ES-BJ MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXrJR|UxRTBwMECwN|c2KM7:TR?= M{nScnNCVkeHUh?=
RH-1 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEHCOmtKSzVyPUCuNFAxOzh4IN88US=> NXTnepRkW0GQR1XS
COLO-800 NUDudoNzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{Luc2lEPTB;MD6wNFA{QDhizszN NXTHRWtKW0GQR1XS
ES7 NEHTXYxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIHobVZKSzVyPUCuNFAxOzh7IN88US=> NUm2[o16W0GQR1XS
GAMG MonnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn7FTWM2OD1yLkCwNFQyPiEQvF2= Mlj4V2FPT0WU
Ca9-22 NWGye3FMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIi4flRKSzVyPUCuNFAxPDJ|IN88US=> MoTGV2FPT0WU
SBC-1 NELPTW9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYTyTJBRUUN3ME2wMlAxODR|IN88US=> NH6zNoxUSU6JRWK=
RPMI-7951 NXq3W2pwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3TRcWlEPTB;MD6wNFA1PDhizszN Mnv0V2FPT0WU
SW1710 NGrr[JpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4HFT2lEPTB;MD6wNFA1PjZizszN MlLmV2FPT0WU
HGC-27 NWX0RXZbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVfJR|UxRTBwMECwOFcyKM7:TR?= NYjJPZlOW0GQR1XS
MC-IXC M1P0Zmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUDpXZBmUUN3ME2wMlAxODR5OTFOwG0> MmDOV2FPT0WU
FTC-133 MmCzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2jDc2lEPTB;MD6wNFA1QDlizszN M3fn[HNCVkeHUh?=
HT-1080 NV3ZeYxWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXzBUpdSUUN3ME2wMlAxODVyMTFOwG0> MVnTRW5ITVJ?
NMC-G1 NETyOI9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVzJR|UxRTBwMECwOVE4KM7:TR?= NHrZVWhUSU6JRWK=
GI-1 NE\ob4xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlfTTWM2OD1yLkCwNFUzQCEQvF2= NWTuO5h2W0GQR1XS
CGTH-W-1 NGr4OJVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Moj0TWM2OD1yLkCwNFU1OiEQvF2= M1\rTnNCVkeHUh?=
A2058 M3XoN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV3JR|UxRTBwMECwOVQ1KM7:TR?= NWHXWYQ6W0GQR1XS
ETK-1 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MluzTWM2OD1yLkCwNFU2QCEQvF2= M2\C[HNCVkeHUh?=
NEC8 NFuwc5ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGrPUZJKSzVyPUCuNFAxPTZzIN88US=> M{LRWXNCVkeHUh?=
TE-11 MoDaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUDJR|UxRTBwMECwOVY6KM7:TR?= MmSyV2FPT0WU
NCI-H720 M3TveWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXfSbopTUUN3ME2wMlAxODV6IN88US=> NHXtNmNUSU6JRWK=
PSN1 M1HiS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWW4SJVnUUN3ME2wMlAxODV6MTFOwG0> M4fCeHNCVkeHUh?=
SW780 NXnGVlR6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF\LZWxKSzVyPUCuNFAxPTh5IN88US=> MlzhV2FPT0WU
8-MG-BA Ml;GS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWrJR|UxRTBwMECwOVg6KM7:TR?= NHvGXXFUSU6JRWK=
EC-GI-10 M{LqUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFKzN2xKSzVyPUCuNFAxPTl{IN88US=> M123bHNCVkeHUh?=
SJSA-1 MlLqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUXKUHExUUN3ME2wMlAxODZzNTFOwG0> NGrGdopUSU6JRWK=
639-V NVfwWpdbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV7PdVV7UUN3ME2wMlAxODZ2NzFOwG0> MVvTRW5ITVJ?
PFSK-1 NX\zRmJpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVjJR|UxRTBwMECwOlU4KM7:TR?= Mn7OV2FPT0WU
DMS-273 NYH6S3YxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYXCenpoUUN3ME2wMlAxODZ5NDFOwG0> MYrTRW5ITVJ?
TE-9 MmS1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYjJR|UxRTBwMECwOlc3KM7:TR?= M336enNCVkeHUh?=
SCC-25 M3fpSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MljpTWM2OD1yLkCwNFY5KM7:TR?= MlX2V2FPT0WU
PA-1 NWTadZVNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2G2T2lEPTB;MD6wNFA3QDdizszN M1\LbnNCVkeHUh?=
MHH-ES-1 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoD2TWM2OD1yLkCwNFch|ryP MnLpV2FPT0WU
Daoy NVXNTlFOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmTnTWM2OD1yLkCwNFch|ryP NXrqdW5IW0GQR1XS
G-402 MnWyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3vM[GlEPTB;MD6wNFA4ODZizszN MmPxV2FPT0WU
YKG-1 NEfz[FBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mk\4TWM2OD1yLkCwNFcxPyEQvF2= MmrtV2FPT0WU
Ca-Ski M4K1UWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVn6U49LUUN3ME2wMlAxODd{ODFOwG0> NYfONYx3W0GQR1XS
CHP-212 NFz3WI5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M33CfGlEPTB;MD6wNFA4OyEQvF2= Mk\KV2FPT0WU
SCC-4 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MULJR|UxRTBwMECwO|M6KM7:TR?= NHfpfWJUSU6JRWK=
EW-3 NXfYcZdzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M17OSGlEPTB;MD6wNFA4PDVizszN Mon2V2FPT0WU
P12-ICHIKAWA NIPEeJhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2DKVWlEPTB;MD6wNFA4PDVizszN NH;3S3ZUSU6JRWK=
HSC-2 M2DZRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUf2eIE1UUN3ME2wMlAxODd3NTFOwG0> MnPQV2FPT0WU
HN M2[4O2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3fjOmlEPTB;MD6wNFA4PThizszN MkfrV2FPT0WU
LXF-289 M1jKW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWrJR|UxRTBwMECwO|U5KM7:TR?= MYPTRW5ITVJ?
ES1 NUDod5BET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV\JR|UxRTBwMECwO|ch|ryP M1y4SXNCVkeHUh?=
NTERA-S-cl-D1 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MojOTWM2OD1yLkCwNFc4OyEQvF2= M{H3e3NCVkeHUh?=
K5 NF;ocoVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mm\hTWM2OD1yLkCwNFc4QCEQvF2= NXiySFZ{W0GQR1XS
HOS NV3yU|AxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFPaN|lKSzVyPUCuNFAxPzh{IN88US=> NWGxRnloW0GQR1XS
YH-13 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkLCTWM2OD1yLkCwNFc6OSEQvF2= NUWw[m02W0GQR1XS
SW954 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{jxemlEPTB;MD6wNFA5OjNizszN NYHjT2prW0GQR1XS
KYSE-510 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NES4enZKSzVyPUCuNFAxQDRizszN MWLTRW5ITVJ?
8505C M3PRS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYXJR|UxRTBwMECwPFU1KM7:TR?= M4ftfnNCVkeHUh?=
A549 NGjaemRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4jrPWlEPTB;MD6wNFA5QDJizszN NYrXbYt{W0GQR1XS
Detroit562 NH3jXIpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4rLemlEPTB;MD6wNFA6ODJizszN NWXEb2NqW0GQR1XS
ES5 MlSyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NW\iN5poUUN3ME2wMlAxODlzIN88US=> M2\LWHNCVkeHUh?=
KYSE-140 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlfUTWM2OD1yLkCwNFk{OyEQvF2= NI[xRVVUSU6JRWK=
GMS-10 NGDhOmZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NU[wZ4tQUUN3ME2wMlAxODl2MTFOwG0> NVXuSJVCW0GQR1XS
D-263MG NH:ydFlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4XVVGlEPTB;MD6wNFA6PDdizszN M1T6Z3NCVkeHUh?=
BCPAP NHzYS5JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2fJeGlEPTB;MD6wNFA6PDdizszN MYnTRW5ITVJ?
CHL-1 MkLOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWrPSmZKUUN3ME2wMlAxODl3ODFOwG0> Mk\NV2FPT0WU
RKO NH7jc3VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV;0ZZZNUUN3ME2wMlAxODl6OTFOwG0> NXfwR|FYW0GQR1XS
SW48 NIPYS2lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXnjZYV5UUN3ME2wMlAxOTBzIN88US=> NGq2enRUSU6JRWK=
BPH-1 M3qwNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3;w[mlEPTB;MD6wNFExOiEQvF2= MWDTRW5ITVJ?
EFO-27 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2jLNmlEPTB;MD6wNFExOiEQvF2= NI\IcYNUSU6JRWK=
CAL-33 NX7RSmtYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHXxRm1KSzVyPUCuNFAyODRizszN MojyV2FPT0WU
NCI-H1792 NHnXdpdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlrHTWM2OD1yLkCwNVA1KM7:TR?= M1;xVXNCVkeHUh?=
BB30-HNC MkHBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2HoU2lEPTB;MD6wNFExPSEQvF2= MWjTRW5ITVJ?
VM-CUB-1 M1f4Smdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV2yblV1UUN3ME2wMlAxOTB3IN88US=> M2DB[HNCVkeHUh?=
TE-5 M1Ls[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlvQTWM2OD1yLkCwNVA3KM7:TR?= MWPTRW5ITVJ?
NB13 MkDnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn65TWM2OD1yLkCwNVA4KM7:TR?= MUTTRW5ITVJ?
NBsusSR M1T4SGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXfrU4hFUUN3ME2wMlAxOTFizszN Mn3uV2FPT0WU
LNCaP-Clone-FGC MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWrJR|UxRTBwMECxNUDPxE1? NHXnVIVUSU6JRWK=
EPLC-272H M2X2RWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYrJR|UxRTBwMECxNVEh|ryP NWXpT4x3W0GQR1XS
KNS-62 MlrHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFrPS29KSzVyPUCuNFAyOTJizszN NFnkS|hUSU6JRWK=
RERF-LC-MS NHj6OFVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn\2TWM2OD1yLkCwNVE{KM7:TR?= MlSyV2FPT0WU
LOXIMVI NWjteIJQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M17TcGlEPTB;MD6wNFEyPiEQvF2= NHrCNFhUSU6JRWK=
KINGS-1 NXzXRY53T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4[xcmlEPTB;MD6wNFEyPyEQvF2= NYTJUlR[W0GQR1XS
T98G MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3PZNWlEPTB;MD6wNFEyPyEQvF2= Ml\uV2FPT0WU
HCC1806 M1m2PGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWPJR|UxRTBwMECxNVgh|ryP NVvYd4dRW0GQR1XS
MZ1-PC Ml\nS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX;QdnRNUUN3ME2wMlAxOTF6IN88US=> M33PRXNCVkeHUh?=
RT-112 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFXFUnpKSzVyPUCuNFAyOTlizszN MmPJV2FPT0WU
LK-2 NH7mcXpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml7jTWM2OD1yLkCwNVIyKM7:TR?= M4DDNnNCVkeHUh?=
A375 M4D4dGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn3QTWM2OD1yLkCwNVIyKM7:TR?= MVnTRW5ITVJ?
SF268 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmfnTWM2OD1yLkCwNVIyKM7:TR?= NXzKb3NGW0GQR1XS
H9 M4i1VGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mmq5TWM2OD1yLkCwNVI1KM7:TR?= MXLTRW5ITVJ?
KYSE-410 MmfRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXT4e3k3UUN3ME2wMlAxOTJ2IN88US=> MYHTRW5ITVJ?
G-401 MojaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmG0TWM2OD1yLkCwNVI4KM7:TR?= MYHTRW5ITVJ?
SW982 NF7tfIRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NG\MfHBKSzVyPUCuNFAyOjdizszN NWPoWHVVW0GQR1XS
D-423MG MoGyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2izeGlEPTB;MD6wNFE{KM7:TR?= MXLTRW5ITVJ?
CAMA-1 NW\YXlBmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWDJR|UxRTBwMECxN|Eh|ryP NUPmPYRYW0GQR1XS
SCC-9 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHHMNlhKSzVyPUCuNFAyOzJizszN M4LDOHNCVkeHUh?=
SAS NFXJd3VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYXJR|UxRTBwMECxN|Qh|ryP NHPSSYZUSU6JRWK=
KYSE-180 M{DsbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV3qSJpvUUN3ME2wMlAxOTN3IN88US=> M{L5NHNCVkeHUh?=
EW-16 NVHrcIJCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWjrZ|I5UUN3ME2wMlAxOTN3IN88US=> MoG0V2FPT0WU
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OVCAR-4 NVOzfFQyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXntS3FIUUN3ME2wMlAxPTh4IN88US=> M2fMUnNCVkeHUh?=
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MOLT-16 NGjWNGJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUHJR|UxRTBwMEC4Nlch|ryP MlvPV2FPT0WU
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NCI-H1693 NYP0Z2hqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MljrTWM2OD1yLkC0N|A2KM7:TR?= MXjTRW5ITVJ?
LAN-6 NGXTXXpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{XUTmlEPTB;MD6wOFM{PSEQvF2= Moi3V2FPT0WU
MDA-MB-175-VII MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYqwVYdCUUN3ME2wMlA1PDF5IN88US=> MWnTRW5ITVJ?
KMOE-2 NVriVWlVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXfK[oxxUUN3ME2wMlA1PDZ7IN88US=> MUHTRW5ITVJ?
DMS-79 NIK2NHJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWnJR|UxRTBwMES1O|kh|ryP M{nmWXNCVkeHUh?=
MHH-NB-11 NFi2N|hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{fVfWlEPTB;MD6wOFYyPCEQvF2= M4HrS3NCVkeHUh?=
NCI-H69 NGG2b4RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYPO[JVVUUN3ME2wMlA1QDR7IN88US=> MmTsV2FPT0WU
KE-37 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmnOTWM2OD1yLkC1NFEh|ryP MYHTRW5ITVJ?
Ramos-2G6-4C10 M3fXU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWfJR|UxRTBwMEWwOlch|ryP NVHMenZ1W0GQR1XS
NCI-H1793 M{nlcWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV;JR|UxRTBwMEWwPVMh|ryP MWfTRW5ITVJ?
HT-1376 NV[3UFJpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1;mT2lEPTB;MD6wOVE5PiEQvF2= M2Cxd3NCVkeHUh?=
EKVX M{TDTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVPldVdIUUN3ME2wMlA2Ozh2IN88US=> NUjxZnVKW0GQR1XS
Capan-2 MnzQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3rLOGlEPTB;MD6wOVM6PiEQvF2= NFHOdI5USU6JRWK=
DoTc2-4510 MmTvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmW1TWM2OD1yLkC1OFQ3KM7:TR?= MnTJV2FPT0WU
UACC-893 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4jS[mlEPTB;MD6wOVQ3OiEQvF2= NIDJ[YhUSU6JRWK=
SK-MEL-3 NXLMe40xT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHHjOWdKSzVyPUCuNFU4QDhizszN NI\QU3BUSU6JRWK=
SK-MEL-1 NILSclFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFvJUY5KSzVyPUCuNFU5KM7:TR?= MUTTRW5ITVJ?
SW948 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYnJR|UxRTBwMEW5PVYh|ryP M1nm[HNCVkeHUh?=
P30-OHK NWHqRmk2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1\KNmlEPTB;MD6wOlE3PyEQvF2= NV\KfppJW0GQR1XS
NH-12 Mo\LS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVLFUIlmUUN3ME2wMlA3OjR2IN88US=> NIf0PHJUSU6JRWK=
KU-19-19 NEXY[5hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn;OTWM2OD1yLkC2N|YyKM7:TR?= MYTTRW5ITVJ?
A431 MnvlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHXLXlBKSzVyPUCuNFY{PzRizszN M3KxWHNCVkeHUh?=
NCI-H2405 MoDKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmnITWM2OD1yLkC2N|g{KM7:TR?= MnXiV2FPT0WU
KARPAS-299 NHPOdGFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV\JR|UxRTBwME[1PFIh|ryP M3HPV3NCVkeHUh?=
BALL-1 NHfPNmlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1i1R2lEPTB;MD6wOlY4PCEQvF2= MW\TRW5ITVJ?
KURAMOCHI NWTJWplMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH\TS3FKSzVyPUCuNFcxPzNizszN NUPRSot4W0GQR1XS
LS-1034 NEDDNJFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUfJR|UxRTBwMEezOlkh|ryP NF3McGlUSU6JRWK=
MFE-280 NWL1PHM5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFjRV5pKSzVyPUCuNFc2OzZizszN NGG2SZFUSU6JRWK=
HOP-62 NEHBVHVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFq1fItKSzVyPUCuNFc3PzZizszN NFzKcJlUSU6JRWK=
KG-1 NU\yPVhwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1T4cWlEPTB;MD6wO|c5PyEQvF2= NFfFXJBUSU6JRWK=
CW-2 NXvQOXlmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVjqbHVCUUN3ME2wMlA4QDN{IN88US=> M1K2T3NCVkeHUh?=
SW837 NV;jN5NrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHmwZo1KSzVyPUCuNFg{PDJizszN NW\rSGVRW0GQR1XS
MFH-ino NH[4Z5NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1PYN2lEPTB;MD6wPFUxOSEQvF2= NGL3O2ZUSU6JRWK=
HCC1419 NVXHboZ2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWrJR|UxRTBwMEi5PFch|ryP MoLDV2FPT0WU
HMV-II MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUfDOWJ5UUN3ME2wMlA6ODFizszN MYjTRW5ITVJ?
BEN MmrxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWXlS|ZzUUN3ME2wMlA6Ozh3IN88US=> NWPvWY1EW0GQR1XS
SK-N-FI MojDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVPk[mh1UUN3ME2wMlA6PTV4IN88US=> MXTTRW5ITVJ?
JVM-2 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn;xTWM2OD1yLkC5O|E3KM7:TR?= NULxOItDW0GQR1XS
CAL-54 NHTXOZlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIrWV2NKSzVyPUCuNVAxPCEQvF2= NFzFXllUSU6JRWK=
T84 NVzHfXJzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV;0Ull1UUN3ME2wMlExQTV{IN88US=> MXPTRW5ITVJ?
SHP-77 NFK0[IdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkDtTWM2OD1yLkGxOlQyKM7:TR?= MkfDV2FPT0WU
G-361 NF3qTZhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MW\JR|UxRTBwMUKzNlQh|ryP NH\wRnJUSU6JRWK=
Calu-3 NYK5eXJ1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnTWTWM2OD1yLkGzPFE3KM7:TR?= NEHETIVUSU6JRWK=
HCC1937 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWfYSHE2UUN3ME2wMlE1QTN5IN88US=> M3PX[XNCVkeHUh?=
KM-H2 NGHzPGhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M33hUGlEPTB;MD6xOVAyKM7:TR?= NUDI[FhbW0GQR1XS
769-P NHryPZBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVjvNYFyUUN3ME2wMlE2QDR|IN88US=> M{XYdXNCVkeHUh?=
NCI-H1304 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnPPTWM2OD1yLkG2NFYyKM7:TR?= MoT3V2FPT0WU
D-542MG MlLlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoLrTWM2OD1yLkG2Nlc5KM7:TR?= NFriUndUSU6JRWK=
ATN-1 NVThTYhtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4X6T2lEPTB;MD6xO|Q1OSEQvF2= MVrTRW5ITVJ?
NCI-H2029 MmfCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWDkbJYzUUN3ME2wMlIxOjR|IN88US=> M1\ZNXNCVkeHUh?=
ZR-75-30 NWTBfFZ1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIDZXWNKSzVyPUCuNlA3PDNizszN MmKwV2FPT0WU
SW1116 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVXrcmV4UUN3ME2wMlIyOTF5IN88US=> NHSyRmdUSU6JRWK=
CCRF-CEM M{fWNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVjibFFnUUN3ME2wMlI4PjF2IN88US=> NUf4NphWW0GQR1XS
LU-135 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYj3TmdSUUN3ME2wMlI4PzN7IN88US=> NEmwWFBUSU6JRWK=
MC116 M1vISWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2HZZ2lEPTB;MD6yPFMxQSEQvF2= MXPTRW5ITVJ?
LS-513 MorYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Moi0TWM2OD1yLkK4O|c{KM7:TR?= Ml;zV2FPT0WU
NCI-H630 NUmwVpF1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mon1TWM2OD1yLkK5N|c3KM7:TR?= NFG0VmJUSU6JRWK=
L-428 MoTzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NETiTJhKSzVyPUCuNlk5QDNizszN MoS4V2FPT0WU
UM-UC-3 NYHVOVNET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYD4fGRvUUN3ME2wMlMyOjlzIN88US=> MXLTRW5ITVJ?
NCI-H1838 M4j3T2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEDVTZRKSzVyPUCuN|E4QDRizszN MWHTRW5ITVJ?
HEL MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYnJR|UxRTBwM{KyOEDPxE1? MmfQV2FPT0WU
OCI-AML2 MlnLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnjmTWM2OD1yLkOyNlk5KM7:TR?= MV7TRW5ITVJ?
A3-KAW MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3jtfmlEPTB;MD6zN|Q4PyEQvF2= M37xUnNCVkeHUh?=
IGROV-1 MkHZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkDXTWM2OD1yLkOzO|U2KM7:TR?= MX7TRW5ITVJ?
Mo-T NEXtZ|ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUPIPFhLUUN3ME2wMlM{QDV|IN88US=> M3jxfXNCVkeHUh?=
HDLM-2 NWHUNYl7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUfac4lXUUN3ME2wMlM{QSEQvF2= M1fuUHNCVkeHUh?=
HCC1569 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVXaWFhYUUN3ME2wMlM1OzB7IN88US=> M1T0PXNCVkeHUh?=
COLO-678 M1P1RWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3GxZ2lEPTB;MD6zO|AxOiEQvF2= M1TpS3NCVkeHUh?=
COR-L105 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2jH[GlEPTB;MD6zO|k{KM7:TR?= MVrTRW5ITVJ?
SK-MEL-24 NIG2SGZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGrpUGZKSzVyPUCuN|g{QDJizszN M1W4WHNCVkeHUh?=
COLO-320-HSR NI\wc|BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlWyTWM2OD1yLkSzPVc3KM7:TR?= MlzTV2FPT0WU
C8166 M1Ht[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnvDTWM2OD1yLkS5NFI{KM7:TR?= Ml;5V2FPT0WU
COLO-679 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV3tclNOUUN3ME2wMlYzOTd{IN88US=> M2rNRXNCVkeHUh?=
TE-12 M1fYXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnfXTWM2OD1yLk[1N|k1KM7:TR?= NXnXdnNzW0GQR1XS
HAL-01 NXL1W5RlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1Ptc2lEPTB;MD62OlIzKM7:TR?= NFXWbGxUSU6JRWK=
H-EMC-SS MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4fsOGlEPTB;MD63NFU1PiEQvF2= MnT3V2FPT0WU
SNU-449 NFfxXHBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3TZNmlEPTB;MD63Nlc1QCEQvF2= NFP6dnBUSU6JRWK=
C32 NVrRO2pIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUDCZ49sUUN3ME2wMlc1OTF{IN88US=> NGrRN4hUSU6JRWK=
K-562 NWXDfVZYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXHG[|Q6UUN3ME2wMlc4ODJ2IN88US=> NFH2OGNUSU6JRWK=
DJM-1 NH23U2ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYfZW3NsUUN3ME2wMlc5PzhzIN88US=> NYq3OI5FW0GQR1XS
RCC10RGB NIroOWlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYDJR|UxRTBwN{mxPFMh|ryP M{PEUXNCVkeHUh?=
CAL-85-1 M4Xxb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1Hj[2lEPTB;MD65NFch|ryP NFHzSoRUSU6JRWK=
NY NX\0NZRET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHLKSmNKSzVyPUCuPVM{PTJizszN M1\MZ3NCVkeHUh?=
NCI-H1993 NV30blExT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXTJR|UxRTFwMUmyPFkh|ryP MU\TRW5ITVJ?
SK-OV-3 M3XERWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4XSVmlEPTB;MT6yNlA5QSEQvF2= NX7iWHE5W0GQR1XS

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
PIM1; 

PubMed: 18426800     


Cells were treated with 100 nm docetaxel for the indicated times. PIM1 and β-ACTIN proteins were analyzed by immunoblot analysis. One of three similar blots is shown. Ratio, ratio of PIM1/β-ACTIN from pooled densitometry data from three separate experimen䲧疝Ỵ疞㧀疜膉痘 瘿뾠ՂᾰƌՂĀ 㺣痖帉痖Ѐ瑖堘𢡄빢᎒ՂĀ鑸᎒彿堙奋堙巫堙᎒ﻺ᎒彿堙ﻮ᎒塚堙ﻺ᎒ꍈ堞빢᎒學堙漸堞圔堙빢᎒圞堙圭堙𢡄玚Wᾰƌ ᾰƌ戤瘯Ɖ⟸෕䐺痖暼瘿⟸෕ᾰƌ

cyclin B; 

PubMed: 18426800     


Immunoblot analysis of cyclin B1 and PIM1 expression after docetaxel 10 nm (left) or 100 nm (right) treatment at various time points.

pSTAT3 / STAT3 ; 

PubMed: 18426800     


Immunoblots of RWPE-2 cells treated with 100 nm docetaxel for the indicated time.

p-p53 / p53; 

PubMed: 22996738     


C4-2 cells were treated with 5 nM docetaxel, the whole cell lysates were collected at different time points and subjected to Western blot.

18426800 22996738
Immunofluorescence
α-tubulin; 

PubMed: 27177833     


Noscapine has no effect on gross microtubule polymerization state but noscapine pre-sensitization followed by docetaxel treatment stabilizes microtubules, hence inhibits cell proliferation. TNBC cells were treated with noscapine (4 µM) or docetaxel (0.4 µ䲧疝Ỵ疞㧀疜膉痘 瘿⟸෕ᾰƌ෕Ð 㺣痖帉痖Ѐ瑖堘𢡄빢᎒෕Ð鑸᎒彿堙奋堙巫堙᎒ﻺ᎒彿堙ﻮ᎒塚堙ﻺ᎒ꍈ堞빢᎒學堙漸堞圔堙빢᎒圞堙圭堙𢡄玚Wᾰƌ ᾰƌ戤瘯Ɖ뾠Ղ䐺痖暼瘿뾠Ղᾰƌ 뾠ՂÐ㺣痖뾠Ղ€𢡄뾤Ղ€䀷痗뾤Ղ౴뾤Ղ㵶痗뾤Ղ뺖᎒泌Itemセ᎒Count﫨呂뿔Ղ猴፲뾤Ղ፲씢痗猸፲髸莤䥷堙᎒セ᎒�堞ﻮ᎒፲露𢡄堚Ҕ齃礤v�堞ﻮ᎒猢Wセ᎒䨼Ą鹿齃殺獸W戀醙

cleaved PARP / cleaved caspase-3; 

PubMed: 21087597     


Immunofluorescence images of PC-3 cells treated with the docetaxel and EM011 schedule treatment. Cells were pretreated with docetaxel for 12 h before addition of EM011 and were fixed at 48 h post docetaxel treatment compared to treatment with EM011 (10 μM䲧疝Ỵ疞㧀疜膉痘 瘿⟸෕ᾰƌ෕Ð 㺣痖帉痖Ѐ瑖堘𢡄빢᎒෕Ð鑸᎒彿堙奋堙巫堙᎒ﻺ᎒彿堙ﻮ᎒塚堙ﻺ᎒ꍈ堞빢᎒學堙漸堞圔堙빢᎒圞堙圭堙𢡄玚Wᾰƌ ᾰƌ戤瘯Ɖ�෋䐺痖暼瘿�෋ᾰƌ �෋à

Acetylated tubulin ; 

PubMed: 18426800     


Immunofluorescence of acetylated tubulin in cells treated with DMSO, 30 nM laulimalide and 30 nM docetaxel for 2.5 h. Tubulin shown in green and DAPI in blue, and the white arrow indicates microtubule bundling. Scale bar represents 10 μm.

SQSTM1 / LAMP1; 

PubMed: 29795139     


AGS cells were treated with docetaxel (10 nM) for 12 h. After immunostaining with SQSTM1 (Alexa Fluor 488, green) and LAMP1 (Alexa Fluor 594, red), cells were examined by fluorescence microscopy (scale bar 20 μm). The colocalization of SQSTM1and LAMP1 was䲧疝Ỵ疞㧀疜膉痘 瘿�෋ᾰƌ෋à 㺣痖帉痖Ѐ

27177833 21087597 18426800 29795139
Growth inhibition assay
Cell viability ; 

PubMed: 22996738     


Docetaxel inhibits the growth of LNCaP, C4-2, DU145, and PC3 prostate cancer cells. Cells were plated in 12-well plates and treated with different concentrations of docetaxel. The cell number was counted after 24 hr. 

22996738
In vivo Docetaxel (33 mg/kg/dose, given i.v. every 4 days for 3 injections) results in a tumor growth delay of 19.3 days in M2OL2 colon xenografts. Docetaxel also shows great antitumor activities in MX-1, SK-MEL-2, LX-1 and OVCAR-3 xenografts. Docetaxel inhibits the angiogenic response to fibroblast growth factor 2 with IC 50 of 5.4 mg/kg when injected twice weekly over a 14-day period, and angiogenesis is completely blocked in mice that receives 10 mg/kg Docetaxel. Docetaxel has selectivity for endothelial cell migration and/or microvessel formation because infiltration of inflammatory cells into the Matrigel plug is much less sensitive to inhibition by Docetaxel. [7]

Protocol

Cell Research:[5]
- Collapse
  • Cell lines: Human gastric cancer cell lines MKN-28
  • Concentrations: 6 nM
  • Incubation Time: 3 days
  • Method: 2000 cells in 180 μL of medium are seeded in a 96-well plate, and 20 μL of drug solution is simultaneously added in triplicate to each well. The plate is incubated for 3 days at 37°C in a humidified atmosphere of 5% CO2. On day 3, 25 μL of MTT reagent is added to each well. After 4 h of incubation, the medium is removed by aspiration. 0.2 mL of dimethylsulphoxide (DMSO) is added to each well and thoroughly mixed by using a mechanical plate mixer to dissolve the resulting MTT-formazan crystals. Absorbance at 540 nm (OD) is measured by a reader.
    (Only for Reference)
Animal Research:[6]
- Collapse
  • Animal Models: Human colon carcinomas xenografts CX-1
  • Formulation: 50 mg/mL stock solution in ethanol by adding an equal volume of polysorbate 80 and diluting with 5% dextrose in water to the final volume
  • Dosages: 33 mg/kg
  • Administration: i.v. every 4 days for 3 injections
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL (123.78 mM)
Ethanol 53 mg/mL warmed (65.6 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+30% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.
2mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 807.88
Formula

C43H53NO14

CAS No. 114977-28-5
Storage powder
in solvent
Synonyms RP56976, NSC 628503
Smiles CC(=O)OC12COC1CC(O)C3(C)C2C(OC(=O)C4=CC=CC=C4)C5(O)CC(OC(=O)C(O)C(NC(=O)OC(C)(C)C)C6=CC=CC=C6)C(=C(C(O)C3=O)C5(C)C)C

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04113005 Not yet recruiting Drug: Desmopressin Castrate Resistance Prostate Cancer Sunnybrook Health Sciences Centre|Sunnybrook Research Institute October 1 2019 Early Phase 1
NCT03700099 Recruiting Drug: Docetaxel|Drug: Enzalutamide Prostate Cancer|Castration-resistant Prostate Cancer Instituto do Cancer do Estado de São Paulo|Astellas Pharma Inc September 3 2019 Phase 2
NCT04067713 Recruiting -- Metastatic Prostate Cancer University College London|Epic Sciences September 20 2019 --
NCT03976375 Recruiting Biological: Pembrolizumab|Drug: Lenvatinib|Drug: Docetaxel Metastatic Non-Small Cell Lung Cancer Merck Sharp & Dohme Corp.|Eisai Inc. June 26 2019 Phase 3

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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