Docetaxel (RP56976)

Name: Docetaxel (RP56976)
Brand: Selleck Chemicals
SKU: S1148
Rating: 5 (129 reviews)

For research use only.

Catalog No.S1148 Synonyms: NSC 628503

129 publications

CAS No. 114977-28-5

Docetaxel (RP56976, NSC 628503), an analog of paclitaxel, is an inhibitor of depolymerisation of microtubules by binding to stabilized microtubules.

Selleck's Docetaxel (RP56976) has been cited by 129 publications

Cancer Cell, 2021, S1535-6108(20)30658-9

PubMed: 33450196 ( click the link to review the publication )

Cell, 2019, 36(2):179-193

PubMed: 28162770 ( click the link to review the publication )

Cancer Cell, 2019, 36(2):179-193

PubMed: 31378681 ( click the link to review the publication )

Cell, 2018, 174(5):1200-1215

PubMed: 30100187 ( click the link to review the publication )

Nat Commun, 2021, 12(1):1812

PubMed: 33753739 ( click the link to review the publication )

Biomaterials, 2021, 272:120770

PubMed: 33798957 ( click the link to review the publication )

J Pathol, 2021, 10.1002/path.5652

PubMed: 33620092 ( click the link to review the publication )

Cancers (Basel), 2021, 13(11)2539

PubMed: 34064221 ( click the link to review the publication )

Mater Sci Eng C Mater Biol Appl, 2021, 124:112039

PubMed: 33947539 ( click the link to review the publication )

Eur J Med Chem, 2021, 211:113003

PubMed: 33248847 ( click the link to review the publication )

J Cell Mol Med, 2021, 10.1111/jcmm.16684

PubMed: 34322995 ( click the link to review the publication )

J Cell Mol Med, 2021, 10.1111/jcmm.16155

PubMed: 33507584 ( click the link to review the publication )

Int J Mol Sci, 2021, 22(8)4098

PubMed: 33921035 ( click the link to review the publication )

Invest New Drugs, 2021, 10.1007/s10637-020-01003-3

PubMed: 33475937 ( click the link to review the publication )

Prostate, 2021, 10.1002/pros.24138

PubMed: 33905554 ( click the link to review the publication )

Chem Biol Interact, 2021, 340:109448

PubMed: 33775687 ( click the link to review the publication )

Molecules, 2021, 26(15)4549

PubMed: 34361708 ( click the link to review the publication )

Clin Res Hepatol Gastroenterol, 2021, 45(5):101586

PubMed: 33662636 ( click the link to review the publication )

Hum Cell, 2021, 10.1007/s13577-021-00579-z

PubMed: 34304386 ( click the link to review the publication )

Mol Med Rep, 2021, 23(1)40

PubMed: 33179075 ( click the link to review the publication )

Adv Biol Regul, 2021, S2212-4926(20)30091-9

PubMed: 33451973 ( click the link to review the publication )

Adv Sci (Weinh), 2020, 7(21):2001018

PubMed: 33173725 ( click the link to review the publication )

Nat Commun, 2020, 10;11(1):2935

PubMed: 32523045 ( click the link to review the publication )

J Immunother Cancer, 2020, 8(2)e000807

PubMed: 33115941 ( click the link to review the publication )
Oncogene, 2020, 10.1038/s41388-020-1168-9

PubMed: 31992853 ( click the link to review the publication )

Cancers (Basel), 2020, 12(12)E3784

PubMed: 33334024 ( click the link to review the publication )

Cancers (Basel), 2020, 12(12)E3869

PubMed: 33371412 ( click the link to review the publication )

Br J Cancer, 2020, 10.1038/s41416-020-01091-1

PubMed: 33024272 ( click the link to review the publication )

Cells, 2020, 9(1)

PubMed: 31947623 ( click the link to review the publication )

Cells, 2020, 9(6):E1474

PubMed: 32560232 ( click the link to review the publication )

Mol Cancer Ther, 2020, 19(1):39-51

PubMed: 31582531 ( click the link to review the publication )

Mol Cancer Ther, 2020, 19(2):364-374

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Am J Cancer Res, 2020, 10(12):4251-4265

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Am J Cancer Res, 2020, 10(8):2649-2676

PubMed: 32905466 ( click the link to review the publication )

Am J Cancer Res, 2020, 10(1):249-262

PubMed: 32064165 ( click the link to review the publication )

Am J Cancer Res, 2020, 10(12):4251-4265

PubMed: 33414998 ( click the link to review the publication )

Am J Cancer Res, 2020, 10(10):3302-3315

PubMed: 33163271 ( click the link to review the publication )

Oncogenesis, 2020, 7;9(5):45

PubMed: 32382014 ( click the link to review the publication )

Front Oncol, 2020, 1.4125

PubMed: 31998647 ( click the link to review the publication )

Front Oncol, 2020, 10:524922

PubMed: 33194583 ( click the link to review the publication )

Int J Mol Sci, 2020, 21(23)E9034

PubMed: 33261142 ( click the link to review the publication )

Int J Biol Sci, 2020, 16(7):1121-1134

PubMed: 32174789 ( click the link to review the publication )

Cancer Cell Int, 2020, 19;20:58

PubMed: 32099531 ( click the link to review the publication )

Exp Cell Res, 2020, 10.1016/j.yexcr.2020.111981

PubMed: 32234375 ( click the link to review the publication )

Toxicol In Vitro, 2020, 104928

PubMed: 32622998 ( click the link to review the publication )

Cancer Chemother Pharmacol, 2020, 10.1007/s00280-020-04187-w

PubMed: 33169187 ( click the link to review the publication )

Hum Cell, 2020, 10.1007/s13577-020-00425-8

PubMed: 32886306 ( click the link to review the publication )

Hum Cell, 2020, 10.1007/s13577-020-00420-z

PubMed: 32870449 ( click the link to review the publication )
Pharmaceutics, 2020, 30;12(5) pii: E413

PubMed: 32366029 ( click the link to review the publication )

Sci Rep, 2020, 10(1):13124

PubMed: 32753665 ( click the link to review the publication )

Cell Death Dis, 2020, 11(7):577

PubMed: 32709853 ( click the link to review the publication )

Cancer Lett, 2020, 470:95-105

PubMed: 31644929 ( click the link to review the publication )

Clin Cancer Res, 2019, 10.1158/1078-0432.CCR-19-0206

PubMed: 31113844 ( click the link to review the publication )

Cell Syst, 2019, 8(2):97-108

PubMed: 30797775 ( click the link to review the publication )

Theranostics, 2019, 9(19):5464-5477

PubMed: 31534497 ( click the link to review the publication )

Cancer Res, 2019, canres.0656.2019

PubMed: 31694904 ( click the link to review the publication )

Cell Rep, 2019, 28(9):2317-2330

PubMed: 31461649 ( click the link to review the publication )

Cell Rep, 2019, 28(13):3367-3380

PubMed: 31553907 ( click the link to review the publication )

Cell Death Differ, 2019, 10.1038/s41418-019-0473-8

PubMed: 31857702 ( click the link to review the publication )

Int J Cancer, 2019, 145(7):1970-1981

PubMed: 30807643 ( click the link to review the publication )

Cancers (Basel), 2019, 11(4)

PubMed: 31018595 ( click the link to review the publication )

Cancers (Basel), 2019, 11(12)E1945

PubMed: 31817360 ( click the link to review the publication )

Cell Death Discov, 2019, 0.30486111111

PubMed: 31583122 ( click the link to review the publication )

Cell Death Dis, 2019, 10(9):660

PubMed: 31506430 ( click the link to review the publication )

Arch Toxicol, 2019, 93(4):953-964

PubMed: 30863990 ( click the link to review the publication )

Cells, 2019, 9(1)

PubMed: 31877673 ( click the link to review the publication )

Mol Cancer Res, 2019, 17(10):2115-2125

PubMed: 31266816 ( click the link to review the publication )

J Virol, 2019, 93(4)

PubMed: 30487274 ( click the link to review the publication )

Sci Rep, 2019, 9(1):17380

PubMed: 31758030 ( click the link to review the publication )

Sci Rep, 2019, 9(1):19384

PubMed: 31852908 ( click the link to review the publication )

Sci Rep, 2019, 9(1):16647

PubMed: 31719636 ( click the link to review the publication )

Mol Cell Endocrinol, 2019, 498:110585

PubMed: 31536779 ( click the link to review the publication )
Clin Exp Metastasis, 2019, 36(2):97-108

PubMed: 30810874 ( click the link to review the publication )

Prostate, 2019, 79(11):1304-1315

PubMed: 31212367 ( click the link to review the publication )

Prostate, 2019, 79(9):1018-1031

PubMed: 31018021 ( click the link to review the publication )

J Cancer Res Clin Oncol, 2019, 145(3):551-559

PubMed: 30474758 ( click the link to review the publication )

Cancer Med, 2019, 10.1002/cam4.2764

PubMed: 31823522 ( click the link to review the publication )

Onco Targets Ther, 2019, 12:9899-9913

PubMed: 31819487 ( click the link to review the publication )

J Org Chem, 2019, 10.1021/acs.joc.9b02414

PubMed: 31858795 ( click the link to review the publication )

Clin Cancer Res, 2018, 24(24):6421-6432

PubMed: 30087142 ( click the link to review the publication )

Cell Death Dis, 2018,

PubMed: 29752436 ( click the link to review the publication )

Mol Cancer Ther, 2018, 17(9):2024-2033

PubMed: 29959202 ( click the link to review the publication )

Sci Rep, 2018, 8(1):14672

PubMed: 30279484 ( click the link to review the publication )

J Biol Chem, 2018, 293(33):12719-12729

PubMed: 29934310 ( click the link to review the publication )

Cancer Manag Res, 2018, 10:6695-6703

PubMed: 30584364 ( click the link to review the publication )

Biomed Pharmacother, 2018, 99:445-450

PubMed: 29665645 ( click the link to review the publication )

Transl Oncol, 2018, 11(6):1406-1418

PubMed: 30219696 ( click the link to review the publication )

Oncol Rep, 2018, 39(4):1901-1909

PubMed: 29393407 ( click the link to review the publication )

Cancer Chemother Pharmacol, 2018, 81(2):255-267

PubMed: 29189915 ( click the link to review the publication )

Gene, 2018, 658:152-158

PubMed: 29518547 ( click the link to review the publication )

Int Urol Nephrol, 2018, 50(4):675-686

PubMed: 29460131 ( click the link to review the publication )

Ann Surg Treat Res, 2018, 95(5):240-248

PubMed: 30402442 ( click the link to review the publication )

Oncotarget, 2018, 9(42):26884-26899

PubMed: 29928491 ( click the link to review the publication )

Nan Fang Yi Ke Da Xue Xue Bao, 2018, 38(6):723-730

PubMed: 29997096 ( click the link to review the publication )

Oncotarget, 2018, 9(56):30773-30786

PubMed: 30112106 ( click the link to review the publication )

Oncotarget, 2018,

PubMed: 29560108 ( click the link to review the publication )
Proc Natl Acad Sci U S A, 2017, 114(18):E3729-E3738

PubMed: 28416665 ( click the link to review the publication )

Cancer Res, 2017, 77(12):3268-3279

PubMed: 28400477 ( click the link to review the publication )

Oncogene, 2017, 36(14):1991-2001

PubMed: 27721412 ( click the link to review the publication )

Oncogene, 2017, 36(11):1573-1584

PubMed: 27617580 ( click the link to review the publication )

Mol Cancer Ther, 2017, 16(11):2552-2562

PubMed: 28847989 ( click the link to review the publication )

Mol Cancer Ther, 2017, 77(22):6282-6298

PubMed: 28424227 ( click the link to review the publication )

Mol Cancer Res, 2017, 15(6):660-669

PubMed: 28209757 ( click the link to review the publication )

J Pharmacol Exp Ther, 2017, 363(1):20-34

PubMed: 28760737 ( click the link to review the publication )

Breast Cancer Res Treat, 2017, 165(3):531-543

PubMed: 28695300 ( click the link to review the publication )

Oncol Lett, 2017, 14(4):4349-4354

PubMed: 28959367 ( click the link to review the publication )

Oncotarget, 2017, 8(67):111225-111245

PubMed: 29340049 ( click the link to review the publication )

Oncotarget, 2017, 9(9):8206-8222

PubMed: 29492189 ( click the link to review the publication )

Oncotarget, 2017, 9(4):4886-4896

PubMed: 29435149 ( click the link to review the publication )

Mol Cancer Ther, 2016, 15(10):2302-2313

PubMed: 27474148 ( click the link to review the publication )

Prostate Cancer Prostatic Dis, 2016, 19(4):349-357

PubMed: 27349498 ( click the link to review the publication )

Int J Cancer, 2015, 136(9):2065-77

PubMed: 25274482 ( click the link to review the publication )

Int J Cancer, 2015, 136(9):2065-77

PubMed: ( click the link to review the publication )

Breast Cancer Res, 2015, 17:59

PubMed: 25902869 ( click the link to review the publication )

Biochem Pharmacol, 2015, 10.1016/j.bcp.2015.05.005

PubMed: ( click the link to review the publication )

Biochem Pharmacol, 2015, 96(3):168-78

PubMed: 25986883 ( click the link to review the publication )

PLoS ONE, 2015,

PubMed: 25969993 ( click the link to review the publication )

PLoS One, 2015, 10(4):e0123901

PubMed: 25875914 ( click the link to review the publication )

Oncotarget, 2015, 6(29):27923-37

PubMed: 26314846 ( click the link to review the publication )

Oncotarget, 2015, 6(27):23358-71

PubMed: 26160840 ( click the link to review the publication )
Oncotarget, 2015, 6(1):56-70

PubMed: 25474141 ( click the link to review the publication )

Oncotarget, 2015, 6(38):41018-32

PubMed: 26512780 ( click the link to review the publication )

Oncogenesis, 2014, 3(10):e124

PubMed: 25347059 ( click the link to review the publication )

Oncogenesis, 2014, 3:e100

PubMed: 24752235 ( click the link to review the publication )

Mol Pharm, 2014, 11(7):2040-50

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J Cell Mol Med, 2014, 18(5):811-23

PubMed: 24628949 ( click the link to review the publication )

Cell Cycle, 2014, 13(20):3292-301

PubMed: 25485509 ( click the link to review the publication )

J Nucl Med, 2013, 54(6):913-21

PubMed: 23564760 ( click the link to review the publication )

J Transl Med, 2013, 11:204

PubMed: 24004449 ( click the link to review the publication )

Purity & Quality Control

Choose Selective Microtubule Associated Inhibitors

Biological Activity

Description

Docetaxel (RP56976, NSC 628503), an analog of paclitaxel, is an inhibitor of depolymerisation of microtubules by binding to stabilized microtubules.

Targets

Microtubules ^[1]
(Cell-free assay)

In vitro

Docetaxel is a cytotoxic agent, especially for proliferating cells, which is related to its ability to promote the formation of microtubule bundles and induce sustained mitotic arrest, followed by apoptosis of mitotically arrested cells or permanent mitotic block. Docetaxel suppresses microtubule dynamic instability as well as tread-milling, resulting in the failure of chromosomes to segregate to the daughter cells, which in turn triggers premature exit from mitosis rather than a block at this phase of the cell cycle. ^[2] Docetaxel inhibits the clonogenic survival of Human cancer cell Hs746T (stomach), AGS (stomach), HeLa (cervix), CaSki (cervix), BxPC3 (pancreas), Capan-1 (pancreas) with IC50 of 1 nM, 1 nM, 0.3 nM, 0.3 nM, 0.3 nM and 0.3 nM respectively. ^[4] Docetaxel inhibits endothelial cell migration that does not affects microtubule gross morphology or inhibit cell proliferation, although they does produce more subtle effects on microtubule dynamics. Docetaxel inhibits HUVEC migration with an observed IC50 of 1 pM. HUVEC chemotaxis stimulated by either of two angiogenic factors, thymidine phosphorylase or VEGF, is inhibited by Docetaxel with IC 50 of 10 pM and is ablated at 1 nM. ^[7] Docetaxel induces human monocytes, but not RAW 264.7 murine macrophages, Prostaglandin H Synthase-2m (PGHS-2) expression. ^[8]

Cell Data

Cell Lines	Assay Type	Activity Description	PMID
HSC-3	NYr4TIxVT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NFu4dphKSzVyPUCuNFAxOTN{IN88US=>	NHfkeHU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6Oi9pPmPBUmdGWjxxYU6=
5637	M17zfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	M1LsTWlEPTB;MD6wNFAyPDNizszN	M2D5O\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFkzNyd-U1HOS2VTRC:jPh?=
HT-144	M2i5Nmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	M3;ZS2lEPTB;MD6wNFAyPTdizszN	NXv2VGx5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQTJxJ{7TRW5ITVJ:L3G+
NCI-H292	NGjNOmVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NWfTOGdXUUN3ME2wMlAxODF3OTFOwG0>	MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5Nk8oRlODTlfFVlww[T5?
HCE-T	MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MVXJR\|UxRTBwMECwNVYzKM7:TR?=	MnfNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPVIwLz6VQV7HSXI9N2F-
ESS-1	NIHLbXVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NWHZVWhFUUN3ME2wMlAxODF5OTFOwG0>	NX\5N5J{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQTJxJ{7TRW5ITVJ:L3G+
D-566MG	NXjK[pJGT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MUDJR\|UxRTBwMECwNVgzKM7:TR?=	NHz5bW09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6Oi9pPmPBUmdGWjxxYU6=
CAL-62	MorZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NWPsPWVvUUN3ME2wMlAxODF6NTFOwG0>	MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5Nk8oRlODTlfFVlww[T5?
GT3TKB	MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	Mk\WTWM2OD1yLkCwNFI{OiEQvF2=	MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5Nk8oRlODTlfFVlww[T5?
HSC-4	MlfLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NXLQWWhtUUN3ME2wMlAxODJ\|OTFOwG0>	MmDMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPVIwLz6VQV7HSXI9N2F-
KOSC-2	NHK1Z4pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MX7JR\|UxRTBwMECwNlU2KM7:TR?=	M3jIdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFkzNyd-U1HOS2VTRC:jPh?=
NB14	MnLVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	M37k[GlEPTB;MD6wNFAzPiEQvF2=	MmH6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPVIwLz6VQV7HSXI9N2F-
ES8	MmjuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	Ml7TTWM2OD1yLkCwNFI3KM7:TR?=	MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5Nk8oRlODTlfFVlww[T5?
BFTC-905	M2XwZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MkHuTWM2OD1yLkCwNFI3QCEQvF2=	NWrSd3M4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQTJxJ{7TRW5ITVJ:L3G+
A498	NVzWc2RZT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MWPJR\|UxRTBwMECwNlc5KM7:TR?=	NEHC[Ig9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6Oi9pPmPBUmdGWjxxYU6=
HO-1-N-1	MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MmPhTWM2OD1yLkCwNFI5OSEQvF2=	NHTl[3Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6Oi9pPmPBUmdGWjxxYU6=
SF539	MkjFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NV3BR3N{UUN3ME2wMlAxODNyMjFOwG0>	MoG1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPVIwLz6VQV7HSXI9N2F-
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IGROV-1	NVPzTHY5T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	M{jjcGlEPTB;MD6zN\|c2PSEQvF2=	NWTKd3FyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQTJxJ{7TRW5ITVJ:L3G+
Mo-T	NHrUO5ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	M4PINGlEPTB;MD6zN\|g2OyEQvF2=	MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5Nk8oRlODTlfFVlww[T5?
HDLM-2	NUTqToROT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MnmwTWM2OD1yLkOzPUDPxE1?	MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5Nk8oRlODTlfFVlww[T5?
HCC1569	NI[1emVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NW\wT2Y2UUN3ME2wMlM1OzB7IN88US=>	NH3BXXI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6Oi9pPmPBUmdGWjxxYU6=
COLO-678	NIDFfG1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	M{\mU2lEPTB;MD6zO\|AxOiEQvF2=	NH7we5k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6Oi9pPmPBUmdGWjxxYU6=
COR-L105	M1HMS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MWjJR\|UxRTBwM{e5N{DPxE1?	MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5Nk8oRlODTlfFVlww[T5?
SK-MEL-24	MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NWX4OFlwUUN3ME2wMlM5Ozh{IN88US=>	MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5Nk8oRlODTlfFVlww[T5?
COLO-320-HSR	MnvlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NYD4TIJnUUN3ME2wMlQ{QTd4IN88US=>	MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5Nk8oRlODTlfFVlww[T5?
C8166	MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MlXYTWM2OD1yLkS5NFI{KM7:TR?=	M4TiNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFkzNyd-U1HOS2VTRC:jPh?=
COLO-679	MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NUjuSYFMUUN3ME2wMlYzOTd{IN88US=>	NVriSWdSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQTJxJ{7TRW5ITVJ:L3G+
TE-12	MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	M4njfWlEPTB;MD62OVM6PCEQvF2=	NXzl[pljRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQTJxJ{7TRW5ITVJ:L3G+
HAL-01	NYDveXFoT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	M2ewNmlEPTB;MD62OlIzKM7:TR?=	NGfLUYY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6Oi9pPmPBUmdGWjxxYU6=
H-EMC-SS	NH;OXHhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NILkS2ZKSzVyPUCuO\|A2PDZizszN	MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5Nk8oRlODTlfFVlww[T5?
SNU-449	M3TZSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NE\POpRKSzVyPUCuO\|I4PDhizszN	MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5Nk8oRlODTlfFVlww[T5?
C32	NVr3dYdPT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	M13YTWlEPTB;MD63OFEyOiEQvF2=	MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5Nk8oRlODTlfFVlww[T5?
K-562	NVLuUVU6T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NVHPeXhsUUN3ME2wMlc4ODJ2IN88US=>	M134W\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFkzNyd-U1HOS2VTRC:jPh?=
DJM-1	Ml;US5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	M1;aN2lEPTB;MD63PFc5OSEQvF2=	M2X3TlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFkzNyd-U1HOS2VTRC:jPh?=
RCC10RGB	MkTLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MVXJR\|UxRTBwN{mxPFMh\|ryP	NYP6TnhsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQTJxJ{7TRW5ITVJ:L3G+
CAL-85-1	NYXVN5dmT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NGX2V5RKSzVyPUCuPVA4KM7:TR?=	M1:xTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFkzNyd-U1HOS2VTRC:jPh?=
NY	M{OwNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	M{jPS2lEPTB;MD65N\|M2OiEQvF2=	NH:wW289[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6Oi9pPmPBUmdGWjxxYU6=
NCI-H1993	MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	M4PnZmlEPTB;MT6xPVI5QSEQvF2=	MlXTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPVIwLz6VQV7HSXI9N2F-
SK-OV-3	M4HKPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MoDZTWM2OD1zLkKyNFg6KM7:TR?=	MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5Nk8oRlODTlfFVlww[T5?

... Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID
Western blot	PIM1; PubMed: 18426800 J Biol Chem Cells were treated with 100 nm docetaxel for the indicated times. PIM1 and β-ACTIN proteins were analyzed by immunoblot analysis. One of three similar blots is shown. Ratio, ratio of PIM1/β-ACTIN from pooled densitometry data from three separate experimen䲧疝Ỵ疞㧀疜膉痘 瘿뾠ՂᾰƌՂĀ 㺣痖帉痖Ѐ瑖堘𢡄빢᎒ՂĀ鑸᎒彿堙奋堙巫堙᎒ﻺ᎒彿堙ﻮ᎒塚堙ﻺ᎒ꍈ堞빢᎒學堙漸堞圔堙빢᎒圞堙圭堙𢡄玚Wᾰƌ ᾰƌ戤瘯Ɖ⟸෕䐺痖暼瘿⟸෕ᾰƌ cyclin B; PubMed: 18426800 J Biol Chem Immunoblot analysis of cyclin B1 and PIM1 expression after docetaxel 10 nm (left) or 100 nm (right) treatment at various time points. pSTAT3 / STAT3 ; PubMed: 18426800 J Biol Chem Immunoblots of RWPE-2 cells treated with 100 nm docetaxel for the indicated time. p-p53 / p53; PubMed: 22996738 Prostate C4-2 cells were treated with 5 nM docetaxel, the whole cell lysates were collected at different time points and subjected to Western blot.	18426800 22996738
Immunofluorescence	α-tubulin; PubMed: 27177833 Exp Cell Res Noscapine has no effect on gross microtubule polymerization state but noscapine pre-sensitization followed by docetaxel treatment stabilizes microtubules, hence inhibits cell proliferation. TNBC cells were treated with noscapine (4 µM) or docetaxel (0.4 µ䲧疝Ỵ疞㧀疜膉痘 瘿⟸෕ᾰƌ෕Ð 㺣痖帉痖Ѐ瑖堘𢡄빢᎒෕Ð鑸᎒彿堙奋堙巫堙᎒ﻺ᎒彿堙ﻮ᎒塚堙ﻺ᎒ꍈ堞빢᎒學堙漸堞圔堙빢᎒圞堙圭堙𢡄玚Wᾰƌ ᾰƌ戤瘯Ɖ뾠Ղ䐺痖暼瘿뾠Ղᾰƌ 뾠ՂÐ㺣痖뾠Ղ𢡄뾤Ղ䀷痗뾤Ղ౴뾤Ղ㵶痗뾤Ղ뺖᎒泌Itemｾ᎒Count﫨呂뿔Ղ猴፲뾤Ղ፲씢痗猸፲髸莤䥷堙᎒ｾ᎒�堞ﻮ᎒፲露𢡄堚Ҕ齃礤v�堞ﻮ᎒猢Wｾ᎒䨼Ą鹿齃殺獸W戀醙 cleaved PARP / cleaved caspase-3; PubMed: 21087597 Biochem Pharmacol Immunofluorescence images of PC-3 cells treated with the docetaxel and EM011 schedule treatment. Cells were pretreated with docetaxel for 12 h before addition of EM011 and were fixed at 48 h post docetaxel treatment compared to treatment with EM011 (10 μM䲧疝Ỵ疞㧀疜膉痘 瘿⟸෕ᾰƌ෕Ð 㺣痖帉痖Ѐ瑖堘𢡄빢᎒෕Ð鑸᎒彿堙奋堙巫堙᎒ﻺ᎒彿堙ﻮ᎒塚堙ﻺ᎒ꍈ堞빢᎒學堙漸堞圔堙빢᎒圞堙圭堙𢡄玚Wᾰƌ ᾰƌ戤瘯Ɖ�෋䐺痖暼瘿�෋ᾰƌ �෋à Acetylated tubulin ; PubMed: 18426800 J Biol Chem Immunofluorescence of acetylated tubulin in cells treated with DMSO, 30 nM laulimalide and 30 nM docetaxel for 2.5 h. Tubulin shown in green and DAPI in blue, and the white arrow indicates microtubule bundling. Scale bar represents 10 μm. SQSTM1 / LAMP1; PubMed: 29795139 Cell Death Dis AGS cells were treated with docetaxel (10 nM) for 12 h. After immunostaining with SQSTM1 (Alexa Fluor 488, green) and LAMP1 (Alexa Fluor 594, red), cells were examined by fluorescence microscopy (scale bar 20 μm). The colocalization of SQSTM1and LAMP1 was䲧疝Ỵ疞㧀疜膉痘 瘿�෋ᾰƌ෋à 㺣痖帉痖Ѐ	27177833 21087597 18426800 29795139
Growth inhibition assay	Cell viability ; PubMed: 22996738 Prostate Docetaxel inhibits the growth of LNCaP, C4-2, DU145, and PC3 prostate cancer cells. Cells were plated in 12-well plates and treated with different concentrations of docetaxel. The cell number was counted after 24 hr.	22996738

In vivo

Docetaxel (33 mg/kg/dose, given i.v. every 4 days for 3 injections) results in a tumor growth delay of 19.3 days in M2OL2 colon xenografts. Docetaxel also shows great antitumor activities in MX-1, SK-MEL-2, LX-1 and OVCAR-3 xenografts. Docetaxel inhibits the angiogenic response to fibroblast growth factor 2 with IC 50 of 5.4 mg/kg when injected twice weekly over a 14-day period, and angiogenesis is completely blocked in mice that receives 10 mg/kg Docetaxel. Docetaxel has selectivity for endothelial cell migration and/or microvessel formation because infiltration of inflammatory cells into the Matrigel plug is much less sensitive to inhibition by Docetaxel. ^[7]

Protocol

Cell Research:^[5]

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Cell lines: Human gastric cancer cell lines MKN-28
Concentrations: 6 nM
Incubation Time: 3 days
Method: 2000 cells in 180 μL of medium are seeded in a 96-well plate, and 20 μL of drug solution is simultaneously added in triplicate to each well. The plate is incubated for 3 days at 37°C in a humidified atmosphere of 5% CO₂. On day 3, 25 μL of MTT reagent is added to each well. After 4 h of incubation, the medium is removed by aspiration. 0.2 mL of dimethylsulphoxide (DMSO) is added to each well and thoroughly mixed by using a mechanical plate mixer to dissolve the resulting MTT-formazan crystals. Absorbance at 540 nm (OD) is measured by a reader.
(Only for Reference)

Animal Research:^[6]

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Animal Models: Human colon carcinomas xenografts CX-1
Dosages: 33 mg/kg
Administration: i.v. every 4 days for 3 injections
(Only for Reference)

References

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Solubility (25°C)

In vitro	DMSO	100 mg/mL (123.78 mM)
	Water	Insoluble
	Ethanol	'53 mg/mL warmed
In vivo	Add solvents to the product individually and in order(Data is from Selleck tests instead of citations): 5% DMSO+30% PEG 300+5% Tween 80+ddH2O For best results, use promptly after mixing.	2mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information Download Docetaxel (RP56976) SDF

Molecular Weight	807.88
Formula	C₄₃H₅₃NO₁₄
CAS No.	114977-28-5
Storage	3 years-20°C powder 2 years-80°C in solvent
Synonyms	NSC 628503
Smiles	CC1=C2C(C(=O)C3(C(CC4C(C3C(C(C2(C)C)(CC1OC(=O)C(C(C5=CC=CC=C5)NC(=O)OC(C)(C)C)O)O)OC(=O)C6=CC=CC=C6)(CO4)OC(=O)C)O)C)O

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Working concentration： mg/ml；

Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL， Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH₂O，mix and clarify.

Note：1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

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C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
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Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2021-09-06)

NCT Number	Recruitment	interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT05027204	Not yet recruiting	Drug: Docetaxel\|Drug: Nivolumab	Squamous Cell Carcinoma of Head and Neck	CSPC ZhongQi Pharmaceutical Technology Co. Ltd.	September 2021	Phase 1\|Phase 2
NCT04467801	Recruiting	Drug: Ipatasertib	NSCLC Stage IV\|NSCLC Stage IIIB	Jun Zhang MD PhD\|University of Iowa\|University of Kentucky\|University of Kansas Medical Center	August 18 2021	Phase 2
NCT04811118	Recruiting	Drug: Docetaxel for Injection (Albumin-bound)\|Drug: Taxotere	Advanced Solid Tumors	CSPC ZhongQi Pharmaceutical Technology Co. Ltd.	May 18 2021	Phase 1
NCT04823910	Not yet recruiting	--	Prostate Cancer	Physiomics\|Portsmouth Hospitals NHS Trust\|National Institute for Health Research United Kingdom	May 2021	--
NCT04889599	Recruiting	Drug: BH009 (Docetaxel injection)\|Drug: Docetaxel injection	Docetaxel\|Solid Tumours\|Bioequivalence	Zhuhai Beihai Biotech Co. Ltd	April 29 2021	Phase 1

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Microtubule Associated Signaling Pathway Map

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Nocodazole (R17934, Oncodazole, NSC238159) is a rapidly-reversible inhibitor of microtubule polymerization, also inhibits Abl, Abl(E255K) and Abl(T315I) with IC50 of 0.21 μM, 0.53 μM and 0.64 μM in cell-free assays, respectively. Nocodazole induces apoptosis.
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Vinorelbine Tartrate (KW-2307, Nor-5'-anhydrovinblastine ditartrate) is a semi-synthetic vinca alkaloid, and inhibits mitosis through interaction with tubulin. Vinorelbine Tartrate exhibits anti-tumor activities via inducing the mitotic apoptosis, autophagy and inflammation.
Cabazitaxel (XRP6258)

Cabazitaxel (RPR-116258A, XRP6258, TXD 258, Taxoid XRP6258) is a semi-synthetic derivative of a natural taxoid that kills cancer cells by inhibiting cell division and growth. Cabazitaxel exerts its effects by inhibiting microtubule growth and assembly, processes that are essential for cells to divide. Cabazitaxel induces autophagy via the PI3K/Akt/mTOR pathway.

Features:A semi-synthetic derivative of a natural taxoid.
Patupilone (Epothilone B)

Patupilone (EPO906, Epothilone B) is a paclitaxel-like microtubule-stabilizing agent with EC_0.01 of 1.8 μM. Phase 2.

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Docetaxel (RP56976)