Cytarabine

Catalog No.S1648

Cytarabine Chemical Structure

Molecular Weight(MW): 243.22

Cytarabine is an antimetabolic agent and DNA synthesis inhibitor with IC50 of 16 nM in wild-type CCRF-CEM cells.

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Cited by 21 Publications

Purity & Quality Control

Choose Selective DNA/RNA Synthesis Inhibitors

Biological Activity

Description Cytarabine is an antimetabolic agent and DNA synthesis inhibitor with IC50 of 16 nM in wild-type CCRF-CEM cells.
Features The 1st of a series of cancer drugs that alters the sugar component of nucleosides.
Targets
DNA synthesis [1]
(CCRF-CEM cells)
16 nM
In vitro

Cytarabine (AraC) is phosphorylated into a triphosphate form (Ara-CTP) involving deoxycytidine kinase (dCK), which competes with dCTP for incorporation into DNA, and then blocks DNA synthesis by inhibiting the function of DNA and RNA polymerases. Cytarabine displays a higher growth inhibitory activity towards wild-type CCRF-CEM cells compared to other acute myelogenous leukemia (AML) cells with IC50 of 16 nM. [1] Increasing concentrations of Cytarabine (IC50 of 0.69 μM) results in decreased metabolic activity of sensitive rat leukemic cell line RO/1, and the cell toxity can be highly enhanced by transfection with human wt dCK (IC50 of 0.037 μM) but not the inactive, alternatively spliced dCK forms. [2] Cytarabine apparently induces apoptosis of rat sympathetic neurons at 10 μM, of which 100 μM shows the highest toxicity and kills over 80% of the neurons by 84 hours, involving the release of mitochondrial cytochrome-c and the activation of caspase-3, and the toxicity can be attenuated by p53 knockdown and delayed by bax deletion. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
mouse L1210 cells NWnkN2Z1S3m2b4TvfIlkyqCjc4PhfS=> NWPOeIZROyCmYYnz M3H5XmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGSnb4j5cpVkdGWxc3nk[UBidmGub3f1[U1{\W6|aYTpeoUhdW:3c3WgUFEzOTBiY3XscJMh[W[2ZYKgN{Bl[Xm|IHL5JG1VXCCjc4PhfUwhUUN3ME2wMlQhdk1w NWXUc5YyOTl4N{S5NFM>
human CCRF-CEM cells MV3DfZRwfG:6aXRCpIF{e2G7 MmXxN{Bl[Xm| NILkd3JEgXSxdH;4bYNqfHliYXfhbY5{fCCmZX;4fY52[2ynb4Pp[IUh[W6jbH;neYUue2Wwc3n0bZZmKGi3bXHuJGNEWkZvQ1XNJINmdGy|IHHmeIVzKDNiZHH5d{BjgSCPVGSgZZN{[XluIFnDOVA:OSCwTT6= MWKxPVY4PDlyMx?=
human MCF7 cells MnHDR5l1d3SxeHnjxsBie3OjeR?= MlHzN{Bl[Xm| MnnSR5l1d3SxeHnjbZR6KGGpYXnud5Qh\GWxeInueYNt\W:|aXTlJIFv[WyxZ4XlMZNmdnOrdHn2[UBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IEOg[IF6eyCkeTDNWHQh[XO|YYmsJGlEPTB;NDDuUU4> M2fieVE6Pjd2OUCz
CCRF-CEM cell line M{fOOmZ2dmO2aX;uJIF{e2G7 MXjJcohq[mm2IITo[UBz\XCuaXPheIlwdiCxZjDDR3JHNUOHTTDj[YxtKGyrbnWgbY4hfmm2cn:sJGVFPTB;NTDuUU4> MX:xPVk2QDl2
human P12-ICHIKAWA cell M1Pvb2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NH75O29KdmirYnn0bY9vKG:oIHj1cYFvKFBzMj3JR2hKU0GZQTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUWuOVQhdk1w NVrNZ|RtW0GQR1XS
human CCRF-CEM cells NF\RSmhEgXSxdH;4bYPDqGG|c3H5 MWK0PEBp MlGwR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gR2NTTi2FRV2gZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KGOnbHygeIl1\XJvYnz1[UBie3OjeTygSWM2OD13Lk[xJI5ONg>? MlrQNVk4PDN6NUi=
CCRF-CEM cell lines NG[yT4tHfW6ldHnvckBie3OjeR?= NHvDTZA4OiCq MojrR49v[2WwdILheIlwdiC{ZYH1bZJm\CC2bzDpcohq[mm2IILldIxq[2G2aX;uJI9nKEOFUl[tR2VOKGOnbHygcIlv\XNiYomgOVAmKGGodHXyJFczKGi{IHnuZ5Vj[XSrb36sJGVFPTB;NjDuUU4> MlnwNVY2OjB{NB?=
human CCRF-CEM cells NHLzTnpEgXSxdH;4bYPDqGG|c3H5 MkLUOFghcA>? NH\iWFlEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBES1KILVPFUUBk\WyuczDhd5Nme3OnZDDhd{Bk\WyuII\pZYJqdGm2eTDh[pRmeiB2ODDodpMh[nliY3XscJRqfGW{LXLseYUh[XO|YYmsJGlEPTB;NjDuUU4> M3v1b|IzPTh{OUmx
human SF268 cells NYDib3A3S3m2b4TvfIlkyqCjc4PhfS=> NG\pVolEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBUTjJ4ODDj[YxteyCkeTDNWHQh[XO|YYmsJGlEPTB;Nz62PEBvVS5? M2L2NVE6OzR3NUix
MOLT-4 human leukemic lymphoblastoid cell lines NHfmeIhEgXSxdH;4bYPDqGG|c3H5 MlrQR5l1d3SxeHnjbZR6KGGpYXnud5QhVU:OVD20JIh2dWGwIHzleYtmdWmlIHz5cZBpd2KuYYP0c4llKGOnbHygcIlv\XNuIFnDOVA:OTBibl2u NGG3cIU3QDJ5NUS2
human 697 cell NI\1dZJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M4jhNWlvcGmkaYTpc44hd2ZiaIXtZY4hPjl5IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUCuOkBvVS5? MUjTRW5ITVJ?
human ES1 cell M2HzcWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M{juZWlvcGmkaYTpc44hd2ZiaIXtZY4hTVNzIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUKuPVchdk1w NXPIcZdtW0GQR1XS
CCRF-CEM human leukemic lymphoblastoid cell lines M37SV2N6fG:2b4jpZ:Kh[XO|YYm= NEfIeZBEgXSxdH;4bYNqfHliYXfhbY5{fCCFQ2LGMWNGVSCqdX3hckBt\XWtZX3pZ{BtgW2yaH;icIF{fG:rZDDj[YxtKGyrbnXzMEBKSzVyPUKwJI5ONg>? NVHLT3JlPjh{N{W0Oi=>
P388 murine cell lines MYnGeY5kfGmxbjDhd5NigQ>? NXXUTodZPzJiaB?= MYrUbIUh[2:vcH;1coQhf2G|IITld5Rm\CCrbjD2bZRzdyCob4KgZY51cXS3bX;yJIFkfGm4aYT5LFUxLSCrbnjpZol1cW:wIH;mJJR2dW:{IHPlcIwh\3Kxd4ToLUBi\2GrboP0JJRp\SCSM{i4JI12emmwZTDj[YxtKGyrbnXzJIFnfGW{IEeyJIhwfXK|LDDJSFUxRTJyIH7NMi=> Mo\tN|YzPTdzNB?=
L1210 murine cell lines NUWzfnVJTnWwY4Tpc44h[XO|YYm= MkHIO|IhcA>? MYXUbIUh[2:vcH;1coQhf2G|IITld5Rm\CCrbjD2bZRzdyCob4KgZY51cXS3bX;yJIFkfGm4aYT5LFUxLSCrbnjpZol1cW:wIH;mJJR2dW:{IHPlcIwh\3Kxd4ToLUBi\2GrboP0JJRp\SCOMUKxNEBufXKrbnWgZ4VtdCCuaX7ld{Bi\nSncjC3NkBpd3W{czygTWQ2OD1{MDDuUU4> MXqzOlI2PzF2
human HEL cell NWTROZhHT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MmHZTY5pcWKrdHnvckBw\iCqdX3hckBJTUxiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1{MD61NkBvVS5? NX;TW411W0GQR1XS
human CCRF-CEM/C2 cells M2\lU2N6fG:2b4jpZ:Kh[XO|YYm= NYThPVZnPDhiaB?= MkLqR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gR2NTTi2FRV2vR|Ih[2WubIOgZYZ1\XJiNEigbJJ{KGK7IHPlcIwhfGm2ZYKtZox2\SCjc4PhfUwhTUN3ME2yNk45KG6PLh?= NV[yfIV2OTl5NEO4OVg>
mouse L1210 cells M3z6O2N6fG:2b4jpZ:Kh[XO|YYm= NUfBXYgxPDhiaB?= NVflc3hES3m2b4TvfIlkcXS7IHHnZYlve3RibX;1d4UhVDF{MUCgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iClZXzsJJBzd2yrZnXyZZRqd25iYX\0[ZIhPDhiaILzMEBKSzVyPUCuNFI1KM7:TT6= NIXSb48zODByMESxPC=>
human CEM cells MlfyR5l1d3SxeHnjxsBie3OjeR?= NVTpTFFUPDhiaB?= NFfSdVNEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBETU1iY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBk\WyuIIDyc4xq\mW{YYTpc44h[W[2ZYKgOFghcHK|LDDJR|UxRTBwMEK0JO69VS5? NYPMW5FwOjByMEC0NVg>
human 2209-23 cells NGf2WnRRem:uaX\ldoF1cW:wIHHzd4F6 MXvBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKDJ{MEmtNlMh[2WubIOgZpkhYzOKXYTofY1q\GmwZTDpcoNwenCxcnH0bY9vKHOlaX70bYxt[XSrb36gdJJwgGmvaYT5JIF{e2G7LDDJR|UxRTBwMEK5JO69VQ>? NWrTc5JiOTlyNUW0PFI>
human CHP-212 cell M3[2Z2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MXjJcohq[mm2aX;uJI9nKGi3bXHuJGNJWC1{MUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zN{41PyCwTT6= MnjEV2FPT0WU
human CTB-1 cell NXH6N4ZlT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M1LsNGlvcGmkaYTpc44hd2ZiaIXtZY4hS1SELUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zOE4{KG6P M17OdHNCVkeHUh?=
human A427 cell NGTVUIJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Mm\pTY5pcWKrdHnvckBw\iCqdX3hckBCPDJ5IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;M{SuOFkhdk1w NVfKNZdSW0GQR1XS
human MOLT-16 cell NFHveHRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NFjYS4tKdmirYnn0bY9vKG:oIHj1cYFvKE2RTGStNVYh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0{Pi5yNzDuUU4> MYLTRW5ITVJ?
human MCF7 1K cells MmDkR5l1d3SxeHnjxsBie3OjeR?= MknyN{Bl[Xm| NIj2XlVEgXSxdH;4bYNqfHliYXfhbY5{fCCmZX;4fY52[2ynb4Pp[IUh[W6jbH;neYUuemW|aYP0ZY51KGi3bXHuJG1ETjdiMVugZ4VtdHNib4\ldoV5eHKnc4Ppcochemmkb371Z4xmd3SrZHWgdoVlfWO2YYPlJIFnfGW{IEOg[IF6eyCkeTDNWHQh[XO|YYmsJGlEPTB;NECgcm0v M{nkelE6Pjd2OUCz
human MC-IXC cell MXfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NG\XXnZKdmirYnn0bY9vKG:oIHj1cYFvKE2FLVnYR{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVQyNjRzIH7NMi=> MU\TRW5ITVJ?
human BHT-101 cell NWO4NIVVT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NG\SO3dKdmirYnn0bY9vKG:oIHj1cYFvKEKKVD2xNFEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF01OS55NjDuUS=> MWLTRW5ITVJ?
human K5 cell MUDHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M2rQfWlvcGmkaYTpc44hd2ZiaIXtZY4hUzViY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD12Mz6yJI5O MkXNV2FPT0WU
parent promyelocytic leukemia cell line NXfXUGNITnWwY4Tpc44h[XO|YYm= Mom0RY51cWOjbnPldkBi[3Srdnn0fUB4[XNiZY\hcJVifGWmIH\vdkB1cGViY3;tdI92dmRiYXfhbY5{fCCyYYLlcpQheHKxbYnlcI9kgXSrYzDs[ZVs\W2rYTDj[YxtKGyrbnWgLGhNNTZyLVfIVHJVMy:mQ1urLUwhTUR3ME20O{BvVS5? NUDmbFRvOzB{N{OyPS=>
human HL60 cells MmXiR5l1d3SxeHnjxsBie3OjeR?= M2XDdWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhNPjBiY3XscJMh[XO|ZYPz[YQh[XNiY3XscEB3cWGkaXzpeJkh[W[2ZYKgOFghcHK|IHL5JINmdGy2aYTldk1jdHWnIHHzd4F6NCCLQ{WwQVAvODR7IN88UU4> MXSyNlU5Ojl7MR?=
human A549 cell Ml\GS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MmPnTY5pcWKrdHnvckBw\iCqdX3hckBCPTR7IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;NEmuOlYhdk1? NXLiN5Z7W0GQR1XS
human NCI-H1703 cell MWPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MnzJTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSEG3NFMh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF01QS56MTDuUS=> MX3TRW5ITVJ?
L1210 cell lines Mn[wSpVv[3Srb36gZZN{[Xl? M{LtTWNwdmOnboTyZZRqd25icnXxeYlz\WRidH:gbY5pcWKrdDDy[ZBtcWOjdHnvckBw\iCOMUKxNEBk\WyuIHzpcoV{KGK7IEWwKUBi\nSncjC3NkBpeiCrbnP1ZoF1cW:wLDDFSFUxRTBwMEWg{txONg>? MVixOlUzODJ2
human K562 cells Mmj5R5l1d3SxeHnjxsBie3OjeR?= MYDDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDLOVYzKGOnbHzzJIJ6KFOUQjDhd5NigSxiSVO1NF0xNjB3IN88US=> NFPuemgzPDl3NkW1Oi=>
human LB2241-RCC cell M1nTZmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MUTJcohq[mm2aX;uJI9nKGi3bXHuJGxDOjJ2MT3SR2Mh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF02PS5|NDDuUS=> NILGNWtUSU6JRWK=
human MLMA cell MlHWS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NFThXpZKdmirYnn0bY9vKG:oIHj1cYFvKE2OTVGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME21OU42OSCwTR?= MVvTRW5ITVJ?
human P30-OHK cell MXvHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NHTGZ4RKdmirYnn0bY9vKG:oIHj1cYFvKFB|MD3PTGsh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF02Pi53IH7N NWrSbWxMW0GQR1XS
RPMI-6410 human leukemic lymphoblastoid cell lines MXnDfZRwfG:6aXRCpIF{e2G7 NXjwTGZZS3m2b4TvfIlkcXS7IHHnZYlve3RiUmDNTU03PDFyIHj1cYFvKGyndXvlcYlkKGy7bYDoc4Jt[XO2b3nkJINmdGxibHnu[ZMtKEmFNUC9NE4xPiEQvF2= MU[2PFI4PTR4
human HT-29 cells MXTQdo9tcW[ncnH0bY9vKGG|c3H5 NIr3[5ZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjUMVI6KGOnbHzzJIFnfGW{IEm2JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MD6wOlYh|ryPLh?= MWqyO|c5PDR7
human NCI-H510A cell Mln4S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MkXoTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSEWxNGEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF03QC5{NjDuUS=> NGriV|JUSU6JRWK=
human J-RT3-T3-5 cell MUfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NGDJWnhKdmirYnn0bY9vKG:oIHj1cYFvKEpvUmSzMXQ{NTViY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD14OD60NkBvVQ>? NX:zSmxZW0GQR1XS
human VA-ES-BJ cell Mkj5S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NWq3ZmFkUW6qaXLpeIlwdiCxZjDoeY1idiCYQT3FV{1DUiClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTdzLk[1JI5O NVK4TXZEW0GQR1XS
human T-24 cell NVq0NIFKT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MmLzTY5pcWKrdHnvckBw\iCqdX3hckBVNTJ2IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;N{iuOVYhdk1? NVjTfmhsW0GQR1XS
human NB69 cell NV25No9VT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NUPnNGl5UW6qaXLpeIlwdiCxZjDoeY1idiCQQk[5JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9O|kvPDhibl2= M4LoTnNCVkeHUh?=
human BALL-1 cells NHPBVYxIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NWDFN3k2OjRiaB?= Mk\CS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gRmFNVC1zIHPlcIx{KGGodHXyJFI1KGi{czDifUBYW1RvMTDhd5NigSxiR1m1NF0xNjB6IN88US=> M4TQTlIzOjZ2MUew
HL60 cells NYnrUph[WHKxbHnm[ZJifGmxbjDhd5NigQ>? NUPtOIp4UW5idnn0do8hcW6qaXLpeI9zgSClb37j[Y51emG2aX;uJIFo[Wmwc4SgdJJwdGmoZYLheIlwdiCxZjDIUFYxKGOnbHzzMEBKSzVyPUCuNFgh|ryP NHfaOY4yOjh5N{W5Ny=>
human CTV-1 cell MlXNS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M1XFV2lvcGmkaYTpc44hd2ZiaIXtZY4hS1SYLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME24OE44PCCwTR?= MUTTRW5ITVJ?
human MEL-JUSO cell MmXHS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NUfre|hjUW6qaXLpeIlwdiCxZjDoeY1idiCPRVytTnVUVyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTh3Lke2JI5O NX[2PWJ3W0GQR1XS
human MOLT-4 cell MVTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M2S2fmlvcGmkaYTpc44hd2ZiaIXtZY4hVU:OVD20JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9PFkvPjdibl2= MlSyV2FPT0WU
human leukemia cell line(CCRF-CEM) NFHJTIZEgXSxdH;4bYPDqGG|c3H5 MXLJckB3cXS{bzDjfZRwfG:6aXPpeJkhd2ZiY3;tdI92dmS|IIfldoUh\GW2ZYLtbY5m\CCxbjDJcohq[mm2aX;uJI9nKGi3bXHuJIxmfWunbXnhJINmdGxibHnu[UhES1KILVPFUUktKEmFNUC9NE4xQSEQvF2u Mk\TPFkyPzZ2NR?=
human SW982 cell NHLoW|RIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXjZeY5bUW6qaXLpeIlwdiCxZjDoeY1idiCVV{m4NkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVkyNjJ|IH7N NEX6R3FUSU6JRWK=
human NCI-SNU-1 cell M2HoSGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1;RXGlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLWPOWU0yKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XoxvJygTWM2OD17Nj63O{BvVQ>? MXvTRW5ITVJ?
HeLa human cell lines MV;GeY5kfGmxbjDhd5NigQ>? MlTvNVQ1KGh? NWf5N5ZtXGinIHPvcZBwfW6mIIfhd{B1\XO2ZXSgbY4hfmm2cn:g[o9zKGGwdHn0eY1weiCjY4Tpeol1gSh3MDWgbY5pcWKrdHnvckBw\iC2dX3vdkBk\WyuIHfyc5d1cCliYXfhbY5{fCCKZVzhJIh2dWGwIHPlcIwhdGmwZYOgZYZ1\XJiMUS0JIhzNCCLREWwQVAvOSEQvF2= MlPTN|YzPTdzNB?=
human H460 cells NEXGWHNHfW6ldHnvckBie3OjeR?= NX;PVHVVOjRiaB?= MV\BcpRqfHWvb4KgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKNE[wJINmdGy|IHHmeIVzKDJ2IHjyd{BjgSCPVGSgZZN{[XoEoB?= NHm5T2IzODJzMUW2OC=>
human HCT116 cells MWnGeY5kfGmxbjDhd5NigQ>? M2PsdWFvfGm2dX3vdkBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjDWFEyPiClZXzsd{Bi\nSncjCyOEBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUCuNUDPxE1? M1XHWFIxOjFzNU[0
human GP5d cell NX\MRmp4T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MX;Jcohq[mm2aX;uJI9nKGi3bXHuJGdRPWRiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zMEKgcm0> NGHib3ZUSU6JRWK=
human SAS cell MWjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MYjJcohq[mm2aX;uJI9nKGi3bXHuJHNCWyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTFyNz61O{BvVQ>? NYHrWlRKW0GQR1XS
human BFTC-905 cell  MnfiS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MX\Jcohq[mm2aX;uJI9nKGi3bXHuJGJHXENvOUC1JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVA5NjNzIH7N M{LsNHNCVkeHUh?=
human LB1047-RCC cell MmjJS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MknFTY5pcWKrdHnvckBw\iCqdX3hckBNSjFyNEetVmNEKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OTB6LkSyJI5O MVLTRW5ITVJ?
human BB65-RCC cell MnPKS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NGixR5NKdmirYnn0bY9vKG:oIHj1cYFvKEKENkWtVmNEKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OTFyLkG5JI5O MmTIV2FPT0WU
human KE-37 cell NUfSfXE1T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NGTDcIxKdmirYnn0bY9vKG:oIHj1cYFvKEuHLUO3JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVExNjh3IH7N MWPTRW5ITVJ?
human NCI-H292 cell MoPoS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? Ml3jTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSEK5NkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEyPC5{IH7N Mof5V2FPT0WU
human HT-1080 cell NUnj[4dDT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MX\Jcohq[mm2aX;uJI9nKGi3bXHuJGhVNTFyOECgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNVQvPDdibl2= NWX0XnZOW0GQR1XS
human HL60 cells NGH3XJFEgXSxdH;4bYPDqGG|c3H5 Ml21OFghcA>? M{K1NmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhNPjBiY3XscJMh[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME2wMlEyQCEQvF2= NUnFV3VLOTl|MkGyN|Q>
human CAKI-1 cell NIXX[ZlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVHHdo94fGhiaX7obYJqfGmxbjDv[kBi\2GrboP0JIh2dWGwIFjDWFEyPiClZXzsd{Bi\nSncjCyOEBpenNiYomgW3NVNTFiYYPzZZktKEeLNUC9NE4yOiEQvF2= MVGyNlI3PDF5MB?=
human CAKI-1 cell NGj0[XJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MYTJcohq[mm2aX;uJI9nKGi3bXHuJGNCU0lvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGyNEBvVQ>? NHW5fG9USU6JRWK=
human HMV-II cell MVnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NVT5OIlEUW6qaXLpeIlwdiCxZjDoeY1idiCKTW[tTWkh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigQ>? MlHaV2FPT0WU
human ES8 cell M3HMR2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MkP5TY5pcWKrdHnvckBw\iCqdX3hckBGWzhiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zMkOuPVMhdk1? M2XUPHNCVkeHUh?=
human NEC8 cell MoPUS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MmPjTY5pcWKrdHnvckBw\iCqdX3hckBPTUN6IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUO0MlIyKG6P MmPXV2FPT0WU
human EW-1 cell NGPId21Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= NETuU5hKdmirYnn0bY9vKG:oIHj1cYFvKEWZLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xN|gvPjJibl2= NVHROYh5W0GQR1XS
murine leukemia P388 MYHGeY5kfGmxbjDhd5NigQ>? MWjBcpRq[2GwY3XyJIFkfGm4aYT5JINienKrZXSgc5V1KGmwII\peJJwKGGpYXnud5QhdXW{aX7lJIxmfWunbXnhJHA{QDhuIFnDOVA:OC5zNDFOwG0> M2\hVFEyOzV4MUGw
human H-EMC-SS cell NVqyc404T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Ml6yTY5pcWKrdHnvckBw\iCqdX3hckBJNUWPQz3TV{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE1PS5{ODDuUS=> NFvKUpNUSU6JRWK=
human SNU638 cells NWDoT3FIS3m2b4TvfIlkyqCjc4PhfS=> NVTmSIZIS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hW06XNkO4JINmdGy|IHL5JHNTSiCjc4PhfUwhUUN3ME2wMlE2KM7:TR?= M1WwOlI1QTV4NUW2
human COR-L105 cell M2j5SWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1y3NGlvcGmkaYTpc44hd2ZiaIXtZY4hS0:ULVyxNFUh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yPjBwM{mgcm0> MlLrV2FPT0WU

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-mTOR / mTOR / p-S6K / S6K / p-AMPK / AMPK / p-AKT / AKT / p-ERK / ERK ; 

PubMed: 24714637     


REH cells were incubated for the indicated time periods with cytarabine (3.2 μM) and the levels of phosphorylated/total mTOR, p70S6K, AMPK, Akt and ERK were determined by immunoblotting. The representative blots from three independent experiments are shown.

24714637
Growth inhibition assay
Cell viability; 

PubMed: 24714637     


REH cells were incubated in the presence of different concentrations of cytarabine and cell viability was determined by acid phosphatase assay after 24 h and 48 h. The data are mean ± SD values of triplicate measurements from a representative of three independent experiments (*p<0.05 compared to untreated cells). 

24714637
In vivo Cytarabine is highly effective against acute leukaemias, which causes the characteristic G1/S blockage and synchronization, and increases the survival time for leukaemic Brown Norway rats in a weak dose-related fashion indicating that the use of higher dosages of Cytarabine does not contribute to its antileukaemic effectiveness in man. [4] Cytarabine (250 mg/kg) also causes placental growth retardation and increases placental trophoblastic cells apoptosis in the placental labyrinth zone of the pregnant Slc:Wistar rats, which increases from 3 hour after the treatment and peaks at 6 hour before returning to control levels at 48 hour, with remarkably enhanced p53 protein, p53 trancriptional target genes such as p21, cyclinG1 and fas and caspase-3 activity. [5]

Protocol

Kinase Assay:[1]
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In Vitro Growth Inhibition Assay:

Stock solution of Cytarabine is prepared in absolute ethanol, and serial dilutions of Cytarabine are prepared. CCRF-CEM cells are suspended in RPMI medium supplemented with 10% FBS, 0.1% gentamicin, and 1% sodium pyruvate. The cells are suspended in their respective media to give 10 mL volumes of cell suspension at a final density of 3-6 × 104 cells/mL. Appropriate volumes of Cytarabine solution are transferred to the cell suspensions, and incubation is continued for 72 hours. The cells are spun down and resuspended in fresh Cytarabine -free medium, and final cell counts are determined. The data are analyzed by sigmoidal curve fitting of the cell count versus Cytarabine concentration, and the results are expressed as the IC50 (Cytarabine concentration that inhibits cell growth to 50% of the control value).
Cell Research:[2]
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  • Cell lines: Rat leukemic cell lines RCL/0, RO/1 and K7 and human myelomonocytic leukemic U937
  • Concentrations: ~100 μM
  • Incubation Time: 24, 48 and 72 hours
  • Method: Cells are incubated in the presence of different concentrations of Cytarabine at 37 °C for 24, 48, and 72 hours. At the time of 20-, 44-, or 68-hour incubation in the presence of Cytarabine, 10 mL of cell proliferation reagent WST-1 solution is added. After 2- and 4-hour incubation with WST-1, cell metabolic activity is assessed with colorimetric changes quantified by measuring the absorbance in a spectrophotometer at 450 nm. And cell division times are calculated from eosin counting in parallel with viability assay
    (Only for Reference)
Animal Research:[4]
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  • Animal Models: Brown Norway rat with myelocytic leukaemia
  • Formulation: Dissolved in phosphate-buffered saline (pH 7.0) just before use.
  • Dosages: 5 - 1000 mg/kg
  • Administration: Injection i.v.
    (Only for Reference)

Solubility (25°C)

In vitro Water 48 mg/mL (197.35 mM)
DMSO 1 mg/mL (4.11 mM)
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
Saline
For best results, use promptly after mixing.
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 243.22
Formula

C9H13N3O5

CAS No. 147-94-4
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

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  • Computed Result

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    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
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    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

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Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04113616 Recruiting Drug: KRT-232|Drug: Cytarabine|Drug: Decitabine Acute Myeloid Leukemia (AML)|Acute Myeloid Leukemia (AML) Secondary to Myeloproliferative Neoplasms (MPN) Kartos Therapeutics Inc. September 25 2019 Phase 1|Phase 2
NCT03878927 Recruiting Drug: CPX-351|Drug: Gemtuzumab Ozogamicin Acute Myeloid Leukemia Weill Medical College of Cornell University|Jazz Pharmaceuticals|Pfizer August 23 2019 Phase 1
NCT03896269 Recruiting Drug: Liposome-encapsulated Daunorubicin-Cytarabine Blasts 10-19 Percent of Bone Marrow Nucleated Cells|Blasts More Than 5 Percent of Bone Marrow Nucleated Cells|High Risk Chronic Myelomonocytic Leukemia|Recurrent Chronic Myelomonocytic Leukemia|Recurrent High Risk Myelodysplastic Syndrome|Refractory Chronic Myelomonocytic Leukemia|Refractory High Risk Myelodysplastic Syndrome M.D. Anderson Cancer Center|National Cancer Institute (NCI) May 14 2019 Phase 1
NCT03348033 Enrolling by invitation Biological: Chronic Myeloid Leukemia + NK cell Chronic Myeloid Leukemia Hospital de Clinicas de Porto Alegre March 2019 Phase 1|Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID