Cytarabine (U-19920A)

Catalog No.S1648 Synonyms: Cytarabin, Ara-C, Arabinofuranosyl Cytidine, Cytosine β-D-arabinofuranoside, Cytosine arabinoside, NSC 63878, NSC 287459

For research use only.

Cytarabine (U-19920A, Cytarabin, Ara-C, Arabinofuranosyl Cytidine, Cytosine β-D-arabinofuranoside, Cytosine arabinoside, NSC 63878, NSC 287459) is an antimetabolic agent and DNA synthesis inhibitor with IC50 of 16 nM in wild-type CCRF-CEM cells. Cytarabine induces autophagy and apoptosis.

Cytarabine (U-19920A) Chemical Structure

CAS No. 147-94-4

Selleck's Cytarabine (U-19920A) has been cited by 61 publications

Purity & Quality Control

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Biological Activity

Description Cytarabine (U-19920A, Cytarabin, Ara-C, Arabinofuranosyl Cytidine, Cytosine β-D-arabinofuranoside, Cytosine arabinoside, NSC 63878, NSC 287459) is an antimetabolic agent and DNA synthesis inhibitor with IC50 of 16 nM in wild-type CCRF-CEM cells. Cytarabine induces autophagy and apoptosis.
Features The 1st of a series of cancer drugs that alters the sugar component of nucleosides.
Targets
DNA synthesis [1]
(CCRF-CEM cells)
16 nM
In vitro

Cytarabine (AraC) is phosphorylated into a triphosphate form (Ara-CTP) involving deoxycytidine kinase (dCK), which competes with dCTP for incorporation into DNA, and then blocks DNA synthesis by inhibiting the function of DNA and RNA polymerases. Cytarabine displays a higher growth inhibitory activity towards wild-type CCRF-CEM cells compared to other acute myelogenous leukemia (AML) cells with IC50 of 16 nM. [1] Increasing concentrations of Cytarabine (IC50 of 0.69 μM) results in decreased metabolic activity of sensitive rat leukemic cell line RO/1, and the cell toxity can be highly enhanced by transfection with human wt dCK (IC50 of 0.037 μM) but not the inactive, alternatively spliced dCK forms. [2] Cytarabine apparently induces apoptosis of rat sympathetic neurons at 10 μM, of which 100 μM shows the highest toxicity and kills over 80% of the neurons by 84 hours, involving the release of mitochondrial cytochrome-c and the activation of caspase-3, and the toxicity can be attenuated by p53 knockdown and delayed by bax deletion. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
murine leukemia P388 MljSSpVv[3Srb36gZZN{[Xl? NVTQOoZZSW62aXPhcoNmeiCjY4Tpeol1gSClYYLybYVlKG:3dDDpckB3cXS{bzDh[4FqdnO2IH31dolv\SCuZYXr[Y1q[SCSM{i4MEBKSzVyPUCuNVQh|ryP Mor3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF|NU[xNVAoRjFzM{W2NVExRC:jPh?=
L1210 cell lines NF3TSVBHfW6ldHnvckBie3OjeR?= NWf2e29NS2:wY3XueJJifGmxbjDy[ZF2cXKnZDD0c{BqdmirYnn0JJJmeGyrY3H0bY9vKG:oIFyxNlExKGOnbHygcIlv\XNiYomgOVAmKGGodHXyJFczKGi{IHnuZ5Vj[XSrb36sJGVFPTB;MD6wOUDPxE1w MorHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ3MkCyOEc,OTZ3MkCyOFww[T5?
CCRF-CEM cell lines MlzkSpVv[3Srb36gZZN{[Xl? M1n1XlczKGh? NUf2WmhqS2:wY3XueJJifGmxbjDy[ZF2cXKnZDD0c{BqdmirYnn0JJJmeGyrY3H0bY9vKG:oIFPDVmYuS0WPIHPlcIwhdGmwZYOgZpkhPTBnIHHmeIVzKDd{IHjyJIlv[3WkYYTpc44tKEWGNUC9OkBvVS5? MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjV{MEK0K|4yPjV{MEK0QE9iRg>?
CCRF-CEM cell line NH\jSY9HfW6ldHnvckBie3OjeR?= MVXJcohq[mm2IITo[UBz\XCuaXPheIlwdiCxZjDDR3JHNUOHTTDj[YxtKGyrbnWgbY4hfmm2cn:sJGVFPTB;NTDuUU4> NEDXWo09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUm1PFk1Lz5zOUm1PFk1RC:jPh?=
HeLa human cell lines NXLRT4t5TnWwY4Tpc44h[XO|YYm= NGDRb4UyPDRiaB?= NGHCNplVcGViY3;tdI92dmRid3HzJJRme3SnZDDpckB3cXS{bzDmc5Ih[W62aYT1cY9zKGGldHn2bZR6MDVyJTDpcohq[mm2aX;uJI9nKHS3bX;yJINmdGxiZ4Lve5RpMSCjZ3HpcpN1KEinTHGgbJVu[W5iY3XscEBtcW6nczDh[pRmeiBzNESgbJItKEmGNUC9NE4yKM7:TR?= M{TJWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzN4MkW3NVQoRjN4MkW3NVQ9N2F-
L1210 murine cell lines M{Hsc2Z2dmO2aX;uJIF{e2G7 NVP2Wpg4PzJiaB?= M3PscnRp\SClb33wc5Vv\CC5YYOgeIV{fGWmIHnuJJZqfHKxIH\vdkBidnSrdIXtc5Ih[WO2aY\peJkpPTBnIHnubIljcXSrb36gc4YhfHWvb4KgZ4VtdCCpcn;3eIgqKGGpYXnud5QhfGinIFyxNlExKG23cnnu[UBk\WyuIHzpcoV{KGGodHXyJFczKGixdYLzMEBKTDVyPUKwJI5ONg>? Mo\iQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzZ{NUexOEc,OzZ{NUexOFww[T5?
P388 murine cell lines M3TjVmZ2dmO2aX;uJIF{e2G7 M2PFR|czKGh? NInJNppVcGViY3;tdI92dmRid3HzJJRme3SnZDDpckB3cXS{bzDmc5Ih[W62aYT1cY9zKGGldHn2bZR6MDVyJTDpcohq[mm2aX;uJI9nKHS3bX;yJINmdGxiZ4Lve5RpMSCjZ3HpcpN1KHSqZTDQN|g5KG23cnnu[UBk\WyuIHzpcoV{KGGodHXyJFczKGixdYLzMEBKTDVyPUKwJI5ONg>? NFvlZo89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|NkK1O|E1Lz5|NkK1O|E1RC:jPh?=
RPMI-6410 human leukemic lymphoblastoid cell lines MUfDfZRwfG:6aXRCpIF{e2G7 MVnDfZRwfG:6aXPpeJkh[WejaX7zeEBTWE2LLU[0NVAhcHWvYX6gcIV2c2WvaXOgcJlueGixYnzhd5RwcWRiY3XscEBtcW6nczygTWM2OD1yLkC2JO69VQ>? M1;1VVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzZ6Mke1OFYoRjZ6Mke1OFY9N2F-
CCRF-CEM human leukemic lymphoblastoid cell lines NHmyXZREgXSxdH;4bYPDqGG|c3H5 M1;uOGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KEOFUl[tR2VOKGi3bXHuJIxmfWunbXnjJIx6dXCqb3LsZZN1d2mmIHPlcIwhdGmwZYOsJGlEPTB;MkCgcm0v M{WwUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzZ6Mke1OFYoRjZ6Mke1OFY9N2F-
MOLT-4 human leukemic lymphoblastoid cell lines M1S0fGN6fG:2b4jpZ:Kh[XO|YYm= MnfuR5l1d3SxeHnjbZR6KGGpYXnud5QhVU:OVD20JIh2dWGwIHzleYtmdWmlIHz5cZBpd2KuYYP0c4llKGOnbHygcIlv\XNuIFnDOVA:OTBibl2u NVq4c5N{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxNkiyO|U1Pid-NkiyO|U1PjxxYU6=
human leukemia cell line(CCRF-CEM) NHrVU3NEgXSxdH;4bYPDqGG|c3H5 M2DMeWlvKH[rdILvJIN6fG:2b4jpZ4l1gSCxZjDjc41xd3WwZIOge4Vz\SCmZYTldo1qdmWmIH;uJGlvcGmkaYTpc44hd2ZiaIXtZY4hdGW3a3XtbYEh[2WubDDsbY5mMEOFUl[tR2VOMSxiSVO1NF0xNjB7IN88UU4> NYnDcXJORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxOEmxO|Y1PSd-OEmxO|Y1PTxxYU6=
HL60 NYPHTYQ6TnWwY4Tpc44h[XO|YYm= NWrBPI9SUW5idnn0do8h[2:wY3XueJJifGmxbjDy[ZF2cXKnZDD0c{BqdmS3Y3WgZZBweHSxc3nzJIlvKEiONkCgZ4VtdHNuIFHDOVAhRSByLkG1JO69VS5? NYnRSVVIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUK4O|c2QTNpPkGyPFc4PTl|PD;hQi=>
HL60R NYfES5p3TnWwY4Tpc44h[XO|YYm= MYfJckB3cXS{bzDjc45k\W62cnH0bY9vKHKncYXpdoVlKHSxIHnu[JVk\SCjcH;weI9{cXNiaX6gTGw3OFJiY3XscJMtKEGFNUCgQUA5KM7:TT6= MmLLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ6N{e1PVMoRjF{OEe3OVk{RC:jPh?=
CCRF-CEM NUi5UHd5S3m2b4TvfIlkcXS7IHHzd4F6 MXi0PEBpenN? NWX2SHp6S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hS0OURj3DSW0h[2WubIOgZYZ1\XJiNEigbJJ{KGK7IHPlcIwhfGm2ZYKtZox2\SCjc4PhfUwhTUN3MDC9JFAvODB3NkG0JO69VS5? MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTd2M{i1PEc,OTl5NEO4OVg9N2F-
CCRF-CEM/C2 NYPJZXhKS3m2b4TvfIlkcXS7IHHzd4F6 MnrPOFghcHK| MWTDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDDR3JHNUOHTT;DNkBk\WyuczDh[pRmeiB2ODDodpMh[nliY3XscEB1cXSncj3icJVmKGG|c3H5MEBGSzVyIE2gNE4xOjJ6IN88UU4> MorYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl5NEO4OVgoRjF7N{SzPFU5RC:jPh?=
HL60/MX2 MoPmR5l1d3SxeHnjbZR6KGG|c3H5 NEL2flM1QCCqcoO= MXTDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIUFYxN02[MjDj[YxteyCjZoTldkA1QCCqcoOgZpkh[2WubDD0bZRmei2kbIXlJIF{e2G7LDDFR|UxKD1iMD6yOlEzKM7:TT6= NYfI[lhDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm3OFM5PThpPkG5O|Q{QDV6PD;hQi=>
HL60 M1XJ[GN6fG:2b4jpZ4l1gSCjc4PhfS=> MUm0PEBpenN? M3LT[mN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhNPjBiY3XscJMh[W[2ZYKgOFghcHK|IHL5JINmdGxidHn0[ZIu[my3ZTDhd5NigSxiRVO1NEA:KDBwNkW3NkDPxE1w M3LNWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7N{SzPFU5Lz5zOUe0N|g2QDxxYU6=
L1210 Mmn5R5l1d3SxeHnjbZR6KGG|c3H5 M{fqeFczKGi{cx?= MXTDfZRwfG:6aXPpeJkh[WejaX7zeEBud3W|ZTDMNVIyOCClZXzsd{Bie3Onc4Pl[EBieyCpcn;3eIghcW6qaXLpeIlwdiCvZXHzeZJm\CCjdDC3NkBpenNiYomgeJJ6eGGwIHLseYUh\XilbIXzbY9vKGG|c3H5MEBGTDVyIE2gNE4yKM7:TT6= NVPIT2hCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUG4NlU5Lz5zMUiyOVg9N2F-
BGM NVKyT5NtSW62aY\pdoFtKGG|c3H5 NVLvSldjPSCmYYnz NUfrbYFkSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhXmGlY3nubYEhfmm{dYOgTWhFKGmwZnXjeIVlKGmwIFHmdolk[W5iZ4Ll[Y4hdW:wa3X5JGJIVSClZXzsd{Bie3Onc4Pl[EBieyCybHHxeYUhemWmdXP0bY9vKG2nYYP1doVlKG:wIHThfUA2KGK7IHTpd4sh\GmoZoXzbY9vKGG|c3H5MEBGTDVyIE2gNE4xPyEQvF2u M1H2WFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5OEi3N{c,OTd6OEezQE9iRg>?
BGM NH7CXXBCdnSrdnnyZYwh[XO|YYm= NFvv[4Q2KGSjeYO= M1vqb2FvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhmenCnczDTbY1xdGW6II\pdpV{KEiIIHnu[oVkfGWmIHnuJGFnemmlYX6g[5Jm\W5ibX;ub4V6KEKJTTDj[YxteyCjc4Pld5Nm\CCjczDwcIFyfWVicnXkeYN1cW:wIH3lZZN2emWmIH;uJIRigSB3IHL5JIRqe2tiZHnm[pV{cW:wIHHzd4F6NCCHREWwJF0hOC5zNjFOwG0v NVvKNplERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe4PFc{Lz5zN{i4O|M9N2F-
L1210 MYLGeY5kfGmxbjDhd5NigQ>? MXjJcohq[mm2aX;uJI9nKHSqeX3p[Jlt[XSnIIP5cpRp[XOnIHnuJGwyOjFyIHPlcIx{NCCHREWwJF0hOC5yMzFOwG0v MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODJ5M{K5K|4{ODJ5M{K5QE9iRg>?
BALL-1 M4fzR2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NVe3eHM{OjRiaILz M4LYZmdzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJGJCVExvMTDj[YxteyCjZoTldkAzPCCqcoOgZpkhX1OWLUGgZZN{[XluIFfJOVAhRSByLkC4JO69VS5? M{HzZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{Mk[0NVcxLz5{MkK2OFE4ODxxYU6=
HCT116 M{PUbWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MkXRNlQhcHK| NYTqXXRGT3Kxd4ToJIlvcGmkaYTpc44hd2ZiYXfhbY5{fCCqdX3hckBJS1RzMU[gZ4VtdHNiYX\0[ZIhOjRiaILzJIJ6KFeVVD2xJIF{e2G7LDDHTVUxKD1iMD6xNkDPxE1w NXvuOnRuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKyOlQyPzBpPkKyNlY1OTdyPD;hQi=>
HeLa NFzHcppIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MmHkNlQhcHK| NI\QPYFIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBJ\UyjIHPlcIx{KGGodHXyJFI1KGi{czDifUBYW1RvMTDhd5NigSxiR1m1NEA:KDBwMUig{txONg>? MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjJ4NEG3NEc,OjJ{NkSxO|A9N2F-
A549 MXfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NXP1OGJLOjRiaILz MVfHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDBOVQ6KGOnbHzzJIFnfGW{IEK0JIhzeyCkeTDXV3QuOSCjc4PhfUwhT0l3MDC9JFAvOiEQvF2u MkjkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ{NkSxO|AoRjJ{Mk[0NVcxRC:jPh?=
HUVEC M3WzfGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NV\QeWpvPzJiaILz MVTHdo94fGhiaX7obYJqfGmxbjDv[kBJXV[HQzDj[YxteyCjZoTldkA4OiCqcoOgZpkhX1OWLUGgZZN{[XluIFfJOVAhRSByLkKzJO69VS5? MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjJ4NEG3NEc,OjJ{NkSxO|A9N2F-
NUGC3 Mn;uS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? Mk\rNlQhcHK| MXnHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDOWWdEOyClZXzsd{Bi\nSncjCyOEBpenNiYomgW3NVNTFiYYPzZZktKEeLNUCgQUAxNjJ3IN88UU4> MofvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ{NkSxO|AoRjJ{Mk[0NVcxRC:jPh?=
HDF NXXj[mNOT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MYK3NkBpenN? MWnHdo94fGhiaX7obYJqfGmxbjDv[kBJTEZiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JHdUXC1zIHHzd4F6NCCJSUWwJF0hOC5{NTFOwG0v M37UcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{Mk[0NVcxLz5{MkK2OFE4ODxxYU6=
leukemia L1210 Mor6SpVv[3Srb36gZZN{[Xl? MXLJcohq[mm2aX;uJI9nKExzMkGwJIx6dXCqb3nkJIxmfWunbXnhJINmdGy|IES4JIhzKGGodHXyJIFldWmwaYP0doF1cW:wIDjs[Y5ofGhib3[gdJJmcW6ldXLheIlwdj1yIHjyLUwhUUN3MDC9JFAvODFizszNMi=> M2P3SFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3Mke3PFMoRjF3Mke3PFM9N2F-
leukemia L1210 MkPoSpVv[3Srb36gZZN{[Xl? NFHtV4tKdmirYnn0bY9vKG:oIFyxNlExKGy7bYDoc4llKGyndXvlcYliKGOnbHzzJFczKGi{IHHmeIVzKGGmbXnubZN1emG2aX;uJEht\W6pdHigc4YheHKnaX7jeYJifGmxbk2wJIhzMSxiSVO1NEA:KDBwMEGg{txONg>? NF3NdZY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUK3O|g{Lz5zNUK3O|g{RC:jPh?=
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Assay
Methods Test Index PMID
Western blot p-mTOR / mTOR / p-S6K / S6K / p-AMPK / AMPK / p-AKT / AKT / p-ERK / ERK 24714637
Growth inhibition assay Cell viability 24714637
In vivo Cytarabine is highly effective against acute leukaemias, which causes the characteristic G1/S blockage and synchronization, and increases the survival time for leukaemic Brown Norway rats in a weak dose-related fashion indicating that the use of higher dosages of Cytarabine does not contribute to its antileukaemic effectiveness in man. [4] Cytarabine (250 mg/kg) also causes placental growth retardation and increases placental trophoblastic cells apoptosis in the placental labyrinth zone of the pregnant Slc:Wistar rats, which increases from 3 hour after the treatment and peaks at 6 hour before returning to control levels at 48 hour, with remarkably enhanced p53 protein, p53 trancriptional target genes such as p21, cyclinG1 and fas and caspase-3 activity. [5]

Protocol (from reference)

Kinase Assay:[1]
  • In Vitro Growth Inhibition Assay:

    Stock solution of Cytarabine is prepared in absolute ethanol, and serial dilutions of Cytarabine are prepared. CCRF-CEM cells are suspended in RPMI medium supplemented with 10% FBS, 0.1% gentamicin, and 1% sodium pyruvate. The cells are suspended in their respective media to give 10 mL volumes of cell suspension at a final density of 3-6 × 104 cells/mL. Appropriate volumes of Cytarabine solution are transferred to the cell suspensions, and incubation is continued for 72 hours. The cells are spun down and resuspended in fresh Cytarabine -free medium, and final cell counts are determined. The data are analyzed by sigmoidal curve fitting of the cell count versus Cytarabine concentration, and the results are expressed as the IC50 (Cytarabine concentration that inhibits cell growth to 50% of the control value).

Cell Research:[2]
  • Cell lines: Rat leukemic cell lines RCL/0, RO/1 and K7 and human myelomonocytic leukemic U937
  • Concentrations: ~100 μM
  • Incubation Time: 24, 48 and 72 hours
  • Method: Cells are incubated in the presence of different concentrations of Cytarabine at 37 °C for 24, 48, and 72 hours. At the time of 20-, 44-, or 68-hour incubation in the presence of Cytarabine, 10 mL of cell proliferation reagent WST-1 solution is added. After 2- and 4-hour incubation with WST-1, cell metabolic activity is assessed with colorimetric changes quantified by measuring the absorbance in a spectrophotometer at 450 nm. And cell division times are calculated from eosin counting in parallel with viability assay
Animal Research:[4]
  • Animal Models: Brown Norway rat with myelocytic leukaemia
  • Dosages: 5 - 1000 mg/kg
  • Administration: Injection i.v.

Solubility (25°C)

In vitro

Water 48 mg/mL
(197.35 mM)
DMSO 1 mg/mL
(4.11 mM)
Ethanol Insoluble

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
Saline
For best results, use promptly after mixing.

30 mg/mL

Chemical Information

Molecular Weight 243.22
Formula

C9H13N3O5

CAS No. 147-94-4
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1=CN(C(=O)N=C1N)C2C(C(C(O2)CO)O)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04526795 Recruiting Drug: Cytarabine|Drug: Fludarabine|Drug: Pegcrisantaspase Blast Phase Chronic Myelogenous Leukemia BCR-ABL1 Positive|Recurrent Acute Biphenotypic Leukemia|Recurrent Acute Lymphoblastic Leukemia|Recurrent Acute Myeloid Leukemia|Recurrent Chronic Myelogenous Leukemia BCR-ABL1 Positive|Recurrent T-Cell Prolymphocytic Leukemia|Refractory Acute Biphenotypic Leukemia|Refractory Acute Lymphoblastic Leukemia|Refractory Acute Myeloid Leukemia|Refractory Chronic Myelogenous Leukemia BCR-ABL1 Positive|Refractory T-Cell Prolymphocytic Leukemia M.D. Anderson Cancer Center|National Cancer Institute (NCI) April 9 2021 Phase 1
NCT04330820 Recruiting Drug: Venetoclax Oral Tablet Relapsed Adult AML Technische Universität Dresden|AbbVie April 6 2020 Phase 1|Phase 2
NCT04253314 Active not recruiting -- Acute Myeloid Leukemia (AML) AbbVie March 13 2020 --

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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