Cytarabine

Catalog No.S1648

Cytarabine Chemical Structure

Molecular Weight(MW): 243.22

Cytarabine is an antimetabolic agent and DNA synthesis inhibitor with IC50 of 16 nM in wild-type CCRF-CEM cells.

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3 Customer Reviews

  • Viability and CI vs Fa after 24-h exposure to cytarabine alone or in combination with ABT-199 in Riva, U2932 and VavP-Bcl2/c-MYC murine tumor cells. Viability shown at 500 nM (500 ng/ml for cytarabine; quadruplicates±s.e.m.).

    Leukemia, 2015, 29(8): 1702–1712. Cytarabine purchased from Selleck.

    Cell cycle was analyzed using propidium iodide (PI) staining and flow cytometry. The plots show PI staining at the x-axis and cell counts at the y-axis. The graphs were prepared using ModFit LT™ software (Verity Software House). Nd: not detected, Ara-c:Cytarabine

    J Exp Clin Cancer Res, 2017, 36(1):22. Cytarabine purchased from Selleck.

  • Flow cytometry analysis for cell surface expression of CD11b shows time- and dose-dependent upregulation of the marker CD11b in MLL-rearranged leukemia cells treated with EPZ-5676 and Cytarabine(Ara-C) as single agents and in combination.

    J Pharmacol Exp Ther, 2014, 350(3): 646-56. Cytarabine purchased from Selleck.

Purity & Quality Control

Choose Selective DNA/RNA Synthesis Inhibitors

Biological Activity

Description Cytarabine is an antimetabolic agent and DNA synthesis inhibitor with IC50 of 16 nM in wild-type CCRF-CEM cells.
Features The 1st of a series of cancer drugs that alters the sugar component of nucleosides.
Targets
DNA synthesis [1]
(CCRF-CEM cells)
16 nM
In vitro

Cytarabine (AraC) is phosphorylated into a triphosphate form (Ara-CTP) involving deoxycytidine kinase (dCK), which competes with dCTP for incorporation into DNA, and then blocks DNA synthesis by inhibiting the function of DNA and RNA polymerases. Cytarabine displays a higher growth inhibitory activity towards wild-type CCRF-CEM cells compared to other acute myelogenous leukemia (AML) cells with IC50 of 16 nM. [1] Increasing concentrations of Cytarabine (IC50 of 0.69 μM) results in decreased metabolic activity of sensitive rat leukemic cell line RO/1, and the cell toxity can be highly enhanced by transfection with human wt dCK (IC50 of 0.037 μM) but not the inactive, alternatively spliced dCK forms. [2] Cytarabine apparently induces apoptosis of rat sympathetic neurons at 10 μM, of which 100 μM shows the highest toxicity and kills over 80% of the neurons by 84 hours, involving the release of mitochondrial cytochrome-c and the activation of caspase-3, and the toxicity can be attenuated by p53 knockdown and delayed by bax deletion. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
mouse L1210 cells NVjNW|FqS3m2b4TvfIlkyqCjc4PhfS=> Mmq1N{Bl[Xm| MUXDfZRwfG:6aXPpeJkh[WejaX7zeEBl\W:6eX71Z4xmd3OrZHWgZY5idG:pdXWtd4Vve2m2aY\lJI1wfXOnIFyxNlExKGOnbHzzJIFnfGW{IEOg[IF6eyCkeTDNWHQh[XO|YYmsJGlEPTB;MD60JI5ONg>? NF3ReIsyQTZ5NEmwNy=>
human CCRF-CEM cells MlrUR5l1d3SxeHnjxsBie3OjeR?= NGW3OVA{KGSjeYO= Mo\sR5l1d3SxeHnjbZR6KGGpYXnud5Qh\GWxeInueYNt\W:|aXTlJIFv[WyxZ4XlMZNmdnOrdHn2[UBpfW2jbjDDR3JHNUOHTTDj[YxteyCjZoTldkA{KGSjeYOgZpkhVVSWIHHzd4F6NCCLQ{WwQVEhdk1w M3ntOlE6Pjd2OUCz
human MCF7 cells NYfNPGFPS3m2b4TvfIlkyqCjc4PhfS=> MV[zJIRigXN? NFTUfHFEgXSxdH;4bYNqfHliYXfhbY5{fCCmZX;4fY52[2ynb4Pp[IUh[W6jbH;neYUue2Wwc3n0bZZmKGi3bXHuJG1ETjdiY3XscJMh[W[2ZYKgN{Bl[Xm|IHL5JG1VXCCjc4PhfUwhUUN3ME20JI5ONg>? MoHpNVk3PzR7MEO=
CCRF-CEM cell line MX\GeY5kfGmxbjDhd5NigQ>? MYLJcohq[mm2IITo[UBz\XCuaXPheIlwdiCxZjDDR3JHNUOHTTDj[YxtKGyrbnWgbY4hfmm2cn:sJGVFPTB;NTDuUU4> MlX0NVk6PTh7NB?=
human P12-ICHIKAWA cell MVvHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? Ml\qTY5pcWKrdHnvckBw\iCqdX3hckBROTJvSVPITWtCX0FiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD13LkW0JI5ONg>? NHuwendUSU6JRWK=
human CCRF-CEM cells M3HKU2N6fG:2b4jpZ:Kh[XO|YYm= MoK5OFghcA>? M2C3bmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGNEWkZvQ1XNJINmdGy|IHHmeIVzKDR6IHjyd{BjgSClZXzsJJRqfGW{LXLseYUh[XO|YYmsJGVEPTB;NT62NUBvVS5? MW[xPVc1Ozh3OB?=
CCRF-CEM cell lines MXzGeY5kfGmxbjDhd5NigQ>? NETsXHA4OiCq NGfO[YFEd26lZX70doF1cW:wIILldZVqemWmIITvJIlvcGmkaYSgdoVxdGmlYYTpc44hd2ZiQ1PSSk1ETU1iY3XscEBtcW6nczDifUA2OCViYX\0[ZIhPzJiaIKgbY5kfWKjdHnvckwhTUR3ME22JI5ONg>? NFPQencyPjV{MEK0
human CCRF-CEM cells Ml\CR5l1d3SxeHnjxsBie3OjeR?= M2rrVlQ5KGh? NYnDdmlZS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hS0OURj3DSW0h[2WubIOgZZN{\XO|ZXSgZZMh[2WubDD2bYFjcWyrdImgZYZ1\XJiNEigbJJ{KGK7IHPlcIx1cXSncj3icJVmKGG|c3H5MEBKSzVyPU[gcm0v M4jIdFIzPTh{OUmx
human SF268 cells M1H2bWN6fG:2b4jpZ:Kh[XO|YYm= NH\EXGhEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBUTjJ4ODDj[YxteyCkeTDNWHQh[XO|YYmsJGlEPTB;Nz62PEBvVS5? NXTORZlWOTl|NEW1PFE>
MOLT-4 human leukemic lymphoblastoid cell lines NIXJNWVEgXSxdH;4bYPDqGG|c3H5 M3TTXGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KE2RTGStOEBpfW2jbjDs[ZVs\W2rYzDsfY1xcG:kbHHzeI9q\CClZXzsJIxqdmW|LDDJR|UxRTFyIH7NMi=> NVTCZZFwPjh{N{W0Oi=>
human 697 cell M{m1WWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MV\Jcohq[mm2aX;uJI9nKGi3bXHuJFY6PyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTFyLk[gcm0v MWLTRW5ITVJ?
human ES1 cell MonTS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MXPJcohq[mm2aX;uJI9nKGi3bXHuJGVUOSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTF{Lkm3JI5ONg>? NFXH[I1USU6JRWK=
CCRF-CEM human leukemic lymphoblastoid cell lines M1nQVWN6fG:2b4jpZ:Kh[XO|YYm= MWHDfZRwfG:6aXPpeJkh[WejaX7zeEBES1KILVPFUUBpfW2jbjDs[ZVs\W2rYzDsfY1xcG:kbHHzeI9q\CClZXzsJIxqdmW|LDDJR|UxRTJyIH7NMi=> NYXvbXhwPjh{N{W0Oi=>
P388 murine cell lines M{LDS2Z2dmO2aX;uJIF{e2G7 NXzSdVVZPzJiaB?= M1HFOHRp\SClb33wc5Vv\CC5YYOgeIV{fGWmIHnuJJZqfHKxIH\vdkBidnSrdIXtc5Ih[WO2aY\peJkpPTBnIHnubIljcXSrb36gc4YhfHWvb4KgZ4VtdCCpcn;3eIgqKGGpYXnud5QhfGinIGCzPFghdXW{aX7lJINmdGxibHnu[ZMh[W[2ZYKgO|IhcG:3coOsJGlFPTB;MkCgcm0v NIDUc2Q{PjJ3N{G0
L1210 murine cell lines NXzZOFdnTnWwY4Tpc44h[XO|YYm= NXjJOoN{PzJiaB?= NYfrXlA3XGinIHPvcZBwfW6mIIfhd{B1\XO2ZXSgbY4hfmm2cn:g[o9zKGGwdHn0eY1weiCjY4Tpeol1gSh3MDWgbY5pcWKrdHnvckBw\iC2dX3vdkBk\WyuIHfyc5d1cCliYXfhbY5{fCC2aHWgUFEzOTBibYXybY5mKGOnbHygcIlv\XNiYX\0[ZIhPzJiaH;1dpMtKEmGNUC9NlAhdk1w MUKzOlI2PzF2
human HEL cell M2DrfWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NYrpZWh5UW6qaXLpeIlwdiCxZjDoeY1idiCKRVygZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yNE42OiCwTT6= NFjJT2NUSU6JRWK=
human CCRF-CEM/C2 cells NH6zRWVEgXSxdH;4bYPDqGG|c3H5 M1yxNlQ5KGh? NEHkN21EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBES1KILVPFUU9EOiClZXzsd{Bi\nSncjC0PEBpenNiYomgZ4VtdCC2aYTldk1jdHWnIHHzd4F6NCCHQ{WwQVIzNjhibl2u Moq4NVk4PDN6NUi=
mouse L1210 cells MmnsR5l1d3SxeHnjxsBie3OjeR?= M{DWTVQ5KGh? MVjDfZRwfG:6aXPpeJkh[WejaX7zeEBud3W|ZTDMNVIyOCClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJINmdGxicILvcIln\XKjdHnvckBi\nSncjC0PEBpenNuIFnDOVA:OC5yMkSg{txONg>? NFfNVXYzODByMESxPC=>
human CEM cells Mn3uR5l1d3SxeHnjxsBie3OjeR?= MVK0PEBp M2LySmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGNGVSClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJINmdGxicILvcIln\XKjdHnvckBi\nSncjC0PEBpenNuIFnDOVA:OC5yMkSg{txONg>? MUWyNFAxODRzOB?=
human 2209-23 cells NH7D[VJRem:uaX\ldoF1cW:wIHHzd4F6 NYX6[HQzSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjCyNlA6NTJ|IHPlcIx{KGK7IGuzTH11cHmvaXTpcoUhcW6lb4Lwc5JifGmxbjDzZ4lvfGmubHH0bY9vKHC{b4jpcYl1gSCjc4PhfUwhUUN3ME2wMlAzQSEQvF2= NEXBXlIyQTB3NUS4Ni=>
human CHP-212 cell Mn\KS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NFzqe3VKdmirYnn0bY9vKG:oIHj1cYFvKEOKUD2yNVIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0{Oy52NzDuUU4> NHSzTo9USU6JRWK=
human CTB-1 cell MknkS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MWfJcohq[mm2aX;uJI9nKGi3bXHuJGNVSi1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;M{SuN{BvVQ>? NWK4V3VLW0GQR1XS
human A427 cell MYjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? Ml;oTY5pcWKrdHnvckBw\iCqdX3hckBCPDJ5IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;M{SuOFkhdk1w NXy5bXFyW0GQR1XS
human MOLT-16 cell M17zOmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NHHjV3NKdmirYnn0bY9vKG:oIHj1cYFvKE2RTGStNVYh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0{Pi5yNzDuUU4> NGC1bYlUSU6JRWK=
human MCF7 1K cells M3G2SmN6fG:2b4jpZ:Kh[XO|YYm= Mmm0N{Bl[Xm| NXmwN4hLS3m2b4TvfIlkcXS7IHHnZYlve3RiZHXvfJlvfWOuZX;zbYRmKGGwYXzv[5VmNXKnc3nzeIFvfCCqdX3hckBOS0Z5IEHLJINmdGy|IH;2[ZJmgHC{ZYPzbY5oKHKrYn;ueYNt\W:2aXTlJJJm\HWldHHz[UBi\nSncjCzJIRigXNiYomgUXRVKGG|c3H5MEBKSzVyPUSwJI5ONg>? M3nvdVE6Pjd2OUCz
human MC-IXC cell M2Xm[Wdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MWrJcohq[mm2aX;uJI9nKGi3bXHuJG1ENUm[QzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUSxMlQyKG6PLh?= MVvTRW5ITVJ?
human BHT-101 cell NUPUdpdHT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M33SUGlvcGmkaYTpc44hd2ZiaIXtZY4hSkiWLUGwNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVQyNjd4IH7N MoPOV2FPT0WU
human K5 cell M{SwXWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MorYTY5pcWKrdHnvckBw\iCqdX3hckBMPSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTR|LkKgcm0> MmrrV2FPT0WU
parent promyelocytic leukemia cell line MlfPSpVv[3Srb36gZZN{[Xl? M3rVc2FvfGmlYX7j[ZIh[WO2aY\peJkhf2G|IHX2ZYx2[XSnZDDmc5IhfGinIHPvcZBwfW6mIHHnZYlve3RicHHy[Y51KHC{b335[Yxw[3m2aXOgcIV2c2WvaXGgZ4VtdCCuaX7lJEhJVC14MD3HTHBTXCtxZFPLL{ktKEWGNUC9OFchdk1w NISwR3g{ODJ5M{K5
human HL60 cells NWXaOm9WS3m2b4TvfIlkyqCjc4PhfS=> MWHDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIUFYxKGOnbHzzJIF{e2W|c3XkJIF{KGOnbHygeoli[mmuaYT5JIFnfGW{IES4JIhzeyCkeTDj[YxtfGm2ZYKtZox2\SCjc4PhfUwhUUN3ME2wMlA1QSEQvF2u NFjuSowzOjV6Mkm5NS=>
human A549 cell MWHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MUnJcohq[mm2aX;uJI9nKGi3bXHuJGE2PDliY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD12OT62OkBvVQ>? M17MPHNCVkeHUh?=
human NCI-H1703 cell MUXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NFfBdoNKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3INVcxOyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTR7LkixJI5O M4POVXNCVkeHUh?=
L1210 cell lines MYnGeY5kfGmxbjDhd5NigQ>? NVTxfIcxS2:wY3XueJJifGmxbjDy[ZF2cXKnZDD0c{BqdmirYnn0JJJmeGyrY3H0bY9vKG:oIFyxNlExKGOnbHygcIlv\XNiYomgOVAmKGGodHXyJFczKGi{IHnuZ5Vj[XSrb36sJGVFPTB;MD6wOUDPxE1w MmjLNVY2OjB{NB?=
human K562 cells NUHub|QxS3m2b4TvfIlkyqCjc4PhfS=> M3raUGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGs2PjJiY3XscJMh[nliU2LCJIF{e2G7LDDJR|UxRTBwMEWg{txO MljoNlQ6PTZ3NU[=
human LB2241-RCC cell MkjOS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MV3Jcohq[mm2aX;uJI9nKGi3bXHuJGxDOjJ2MT3SR2Mh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF02PS5|NDDuUS=> NInaSm1USU6JRWK=
human MLMA cell MY\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M{TpbWlvcGmkaYTpc44hd2ZiaIXtZY4hVUyPQTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUW1MlUyKG6P NYTzfoRrW0GQR1XS
human P30-OHK cell MXrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MlfoTY5pcWKrdHnvckBw\iCqdX3hckBROzBvT1jLJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OVYvPSCwTR?= MWHTRW5ITVJ?
RPMI-6410 human leukemic lymphoblastoid cell lines MX7DfZRwfG:6aXRCpIF{e2G7 Mk\pR5l1d3SxeHnjbZR6KGGpYXnud5QhWlCPST22OFExKGi3bXHuJIxmfWunbXnjJIx6dXCqb3LsZZN1d2mmIHPlcIwhdGmwZYOsJGlEPTB;MD6wOkDPxE1? M2Xqd|Y5Ojd3NE[=
human HT-29 cells M4HKOnBzd2yrZnXyZZRqd25iYYPzZZk> Mnv3RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKVD2yPUBk\WyuczDh[pRmeiB7NjDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTBwME[2JO69VS5? M3vzTVI4Pzh2NEm=
human NCI-H510A cell NGrZR5lIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MnTZTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSEWxNGEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF03QC5{NjDuUS=> Ml\zV2FPT0WU
human J-RT3-T3-5 cell MXzHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MVnJcohq[mm2aX;uJI9nKGi3bXHuJGouWlR|LWSzMVUh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF03QC52MjDuUS=> M4PPTnNCVkeHUh?=
human VA-ES-BJ cell MlTXS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MmTpTY5pcWKrdHnvckBw\iCqdX3hckBXSS2HUz3CTkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVcyNjZ3IH7N Mof5V2FPT0WU
human T-24 cell NGLNfG1Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= MYfJcohq[mm2aX;uJI9nKGi3bXHuJHQuOjRiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD15OD61OkBvVQ>? MnTOV2FPT0WU
human NB69 cell NWfkeXVtT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MVTJcohq[mm2aX;uJI9nKGi3bXHuJG5DPjliY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD15OT60PEBvVQ>? MkTIV2FPT0WU
human BALL-1 cells MVrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NUPVVJNlOjRiaB?= MoS5S5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gRmFNVC1zIHPlcIx{KGGodHXyJFI1KGi{czDifUBYW1RvMTDhd5NigSxiR1m1NF0xNjB6IN88US=> MYGyNlI3PDF5MB?=
HL60 cells Mnz0VJJwdGmoZYLheIlwdiCjc4PhfS=> NFjSflNKdiC4aYTyc{BqdmirYnn0c5J6KGOxbnPlcpRz[XSrb36gZYdicW6|dDDwdo9tcW[ncnH0bY9vKG:oIFjMOlAh[2WubIOsJGlEPTB;MD6wPEDPxE1? MlrFNVI5Pzd3OUO=
human CTV-1 cell MWPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MYXJcohq[mm2aX;uJI9nKGi3bXHuJGNVXi1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;OESuO|Qhdk1? MlPjV2FPT0WU
human MEL-JUSO cell MVXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NYHHWnpiUW6qaXLpeIlwdiCxZjDoeY1idiCPRVytTnVUVyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTh3Lke2JI5O NEPtRVBUSU6JRWK=
human MOLT-4 cell Mn:zS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MXXJcohq[mm2aX;uJI9nKGi3bXHuJG1QVFRvNDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUi5MlY4KG6P NWHDVWRwW0GQR1XS
human leukemia cell line(CCRF-CEM) NEe1bZpEgXSxdH;4bYPDqGG|c3H5 NEDEfGxKdiC4aYTyc{BkgXSxdH;4bYNqfHlib3[gZ49ueG:3bnTzJJdmemViZHX0[ZJucW6nZDDvckBKdmirYnn0bY9vKG:oIHj1cYFvKGyndXvlcYliKGOnbHygcIlv\SiFQ2LGMWNGVSluIFnDOVA:OC5yOTFOwG0v MXG4PVE4PjR3
human SW982 cell NETYRopIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NWX0SIJMUW6qaXLpeIlwdiCxZjDoeY1idiCVV{m4NkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVkyNjJ|IH7N NI\DWpZUSU6JRWK=
human NCI-SNU-1 cell MoTHS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NHXCPGZKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3TUnUuOSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G778{MJGlEPTB;OU[uO|chdk1? MYrTRW5ITVJ?
HeLa human cell lines NUPGWWdGTnWwY4Tpc44h[XO|YYm= NV3ZSnhCOTR2IHi= NXzOOYxoXGinIHPvcZBwfW6mIIfhd{B1\XO2ZXSgbY4hfmm2cn:g[o9zKGGwdHn0eY1weiCjY4Tpeol1gSh3MDWgbY5pcWKrdHnvckBw\iC2dX3vdkBk\WyuIHfyc5d1cCliYXfhbY5{fCCKZVzhJIh2dWGwIHPlcIwhdGmwZYOgZYZ1\XJiMUS0JIhzNCCLREWwQVAvOSEQvF2= NEDaRoc{PjJ3N{G0
human H460 cells NXfLUW01TnWwY4Tpc44h[XO|YYm= MkjiNlQhcA>? NVf1PYhtSW62aYT1cY9zKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gTFQ3OCClZXzsd{Bi\nSncjCyOEBpenNiYomgUXRVKGG|c3H5xsA> NFrKWGszODJzMUW2OC=>
human HCT116 cells M4[1WWZ2dmO2aX;uJIF{e2G7 Mmq3RY51cXS3bX;yJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSFPUNVE3KGOnbHzzJIFnfGW{IEK0JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MD6xJO69VQ>? MYOyNFIyOTV4NB?=
human GP5d cell NX64TGVDT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MWXJcohq[mm2aX;uJI9nKGi3bXHuJGdRPWRiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zMEKgcm0> MnSyV2FPT0WU
human SAS cell NIjyU3dIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NIn6OYZKdmirYnn0bY9vKG:oIHj1cYFvKFODUzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGwO{42PyCwTR?= MYPTRW5ITVJ?
human BFTC-905 cell  M4nNeGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NFT1N3VKdmirYnn0bY9vKG:oIHj1cYFvKEKIVFOtPVA2KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OTB6LkOxJI5O NVG1XFlIW0GQR1XS
human LB1047-RCC cell M3H2Zmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M{C1N2lvcGmkaYTpc44hd2ZiaIXtZY4hVEJzMES3MXJESyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTFyOD60NkBvVQ>? M2LHd3NCVkeHUh?=
human BB65-RCC cell NFXoeXFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NH7rb3lKdmirYnn0bY9vKG:oIHj1cYFvKEKENkWtVmNEKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OTFyLkG5JI5O MmjhV2FPT0WU
human KE-37 cell M2mxd2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NIjrXlBKdmirYnn0bY9vKG:oIHj1cYFvKEuHLUO3JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVExNjh3IH7N NGnXNZdUSU6JRWK=
human NCI-H292 cell MmC0S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M33KTmlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLViyPVIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yOTRwMjDuUS=> NFe3N3lUSU6JRWK=
human HT-1080 cell MXPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M4DsfWlvcGmkaYTpc44hd2ZiaIXtZY4hUFRvMUC4NEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEyPC52NzDuUS=> NHfnUWRUSU6JRWK=
human HL60 cells MY\DfZRwfG:6aXRCpIF{e2G7 NHzuZXY1QCCq MkO4R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTGw3OCClZXzsd{Bi\nSncjC0PEBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUCuNVE5KM7:TR?= Mne1NVk{OjF{M{S=
human CAKI-1 cell NE\XVllIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXqyT4pnT3Kxd4ToJIlvcGmkaYTpc44hd2ZiYXfhbY5{fCCqdX3hckBJS1RzMU[gZ4VtdHNiYX\0[ZIhOjRiaILzJIJ6KFeVVD2xJIF{e2G7LDDHTVUxRTBwMUKg{txO NF7ZU3ozOjJ4NEG3NC=>
human CAKI-1 cell M1i1fGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NYr6[JJMUW6qaXLpeIlwdiCxZjDoeY1idiCFQVvJMVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yOjBibl2= M2nQSnNCVkeHUh?=
human HMV-II cell MlnOS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? Mmq5TY5pcWKrdHnvckBw\iCqdX3hckBJVVZvSVmgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfS=> M1jodnNCVkeHUh?=
human ES8 cell MoLjS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MVvJcohq[mm2aX;uJI9nKGi3bXHuJGVUQCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTF{Mz65N{BvVQ>? NWq0d29GW0GQR1XS
human NEC8 cell M{HWRmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MWPJcohq[mm2aX;uJI9nKGi3bXHuJG5GSzhiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zM{SuNlEhdk1? NFHT[4FUSU6JRWK=
human EW-1 cell MonwS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NEnXPYxKdmirYnn0bY9vKG:oIHj1cYFvKEWZLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xN|gvPjJibl2= NUnNdJV[W0GQR1XS
murine leukemia P388 NWi5OXRITnWwY4Tpc44h[XO|YYm= NWD0W2lJSW62aXPhcoNmeiCjY4Tpeol1gSClYYLybYVlKG:3dDDpckB3cXS{bzDh[4FqdnO2IH31dolv\SCuZYXr[Y1q[SCSM{i4MEBKSzVyPUCuNVQh|ryP MUSxNVM2PjFzMB?=
human H-EMC-SS cell M3zSWGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MlGyTY5pcWKrdHnvckBw\iCqdX3hckBJNUWPQz3TV{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE1PS5{ODDuUS=> MnnIV2FPT0WU
human SNU638 cells Mo\1R5l1d3SxeHnjxsBie3OjeR?= MknLR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gV25WPjN6IHPlcIx{KGK7IGPSRkBie3OjeTygTWM2OD1yLkG1JO69VQ>? MWGyOFk2PjV3Nh?=
human COR-L105 cell MojtS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MXTJcohq[mm2aX;uJI9nKGi3bXHuJGNQWi2OMUC1JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVYxNjN7IH7N NHLSco1USU6JRWK=

... Click to View More Cell Line Experimental Data

In vivo Cytarabine is highly effective against acute leukaemias, which causes the characteristic G1/S blockage and synchronization, and increases the survival time for leukaemic Brown Norway rats in a weak dose-related fashion indicating that the use of higher dosages of Cytarabine does not contribute to its antileukaemic effectiveness in man. [4] Cytarabine (250 mg/kg) also causes placental growth retardation and increases placental trophoblastic cells apoptosis in the placental labyrinth zone of the pregnant Slc:Wistar rats, which increases from 3 hour after the treatment and peaks at 6 hour before returning to control levels at 48 hour, with remarkably enhanced p53 protein, p53 trancriptional target genes such as p21, cyclinG1 and fas and caspase-3 activity. [5]

Protocol

Kinase Assay:[1]
+ Expand

In Vitro Growth Inhibition Assay:

Stock solution of Cytarabine is prepared in absolute ethanol, and serial dilutions of Cytarabine are prepared. CCRF-CEM cells are suspended in RPMI medium supplemented with 10% FBS, 0.1% gentamicin, and 1% sodium pyruvate. The cells are suspended in their respective media to give 10 mL volumes of cell suspension at a final density of 3-6 × 104 cells/mL. Appropriate volumes of Cytarabine solution are transferred to the cell suspensions, and incubation is continued for 72 hours. The cells are spun down and resuspended in fresh Cytarabine -free medium, and final cell counts are determined. The data are analyzed by sigmoidal curve fitting of the cell count versus Cytarabine concentration, and the results are expressed as the IC50 (Cytarabine concentration that inhibits cell growth to 50% of the control value).
Cell Research:[2]
+ Expand
  • Cell lines: Rat leukemic cell lines RCL/0, RO/1 and K7 and human myelomonocytic leukemic U937
  • Concentrations: ~100 μM
  • Incubation Time: 24, 48 and 72 hours
  • Method: Cells are incubated in the presence of different concentrations of Cytarabine at 37 °C for 24, 48, and 72 hours. At the time of 20-, 44-, or 68-hour incubation in the presence of Cytarabine, 10 mL of cell proliferation reagent WST-1 solution is added. After 2- and 4-hour incubation with WST-1, cell metabolic activity is assessed with colorimetric changes quantified by measuring the absorbance in a spectrophotometer at 450 nm. And cell division times are calculated from eosin counting in parallel with viability assay
    (Only for Reference)
Animal Research:[4]
+ Expand
  • Animal Models: Brown Norway rat with myelocytic leukaemia
  • Formulation: Dissolved in phosphate-buffered saline (pH 7.0) just before use.
  • Dosages: 5 - 1000 mg/kg
  • Administration: Injection i.v.
    (Only for Reference)

Solubility (25°C)

In vitro Water 48 mg/mL (197.35 mM)
DMSO 1 mg/mL (4.11 mM)
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
Saline
For best results, use promptly after mixing.
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 243.22
Formula

C9H13N3O5

CAS No. 147-94-4
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
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    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

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Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

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Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03571321 Not yet recruiting Acute Lymphoblastic Leukemia|ALL Childhood|ALL University of Chicago|Incyte Corporation September 5 2019 Phase 1
NCT03719560 Not yet recruiting Diffuse Large B Cell Lymphoma Brown University July 1 2019 Phase 2
NCT03634228 Not yet recruiting Acute Myeloid Leukemia Arising From Previous Myelodysplastic Syndrome|Recurrent Acute Myeloid Leukemia|Refractory Acute Myeloid Leukemia|TP53 Gene Mutation Negative M.D. Anderson Cancer Center|National Cancer Institute (NCI) January 31 2019 Phase 1|Phase 2
NCT03724084 Not yet recruiting Acute Myeloid Leukemia|KMT2A Gene Rearrangement National Cancer Institute (NCI) January 25 2019 Phase 1|Phase 2
NCT03710772 Not yet recruiting CD20 Positive|Mantle Cell Lymphoma M.D. Anderson Cancer Center|National Cancer Institute (NCI) January 31 2019 Phase 2
NCT03701308 Not yet recruiting Acute Myeloid Leukemia National Cancer Institute (NCI) January 16 2019 Phase 2|Phase 3

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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DNA/RNA Synthesis Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID