Cytarabine

Catalog No.S1648

Cytarabine Chemical Structure

Molecular Weight(MW): 243.22

Cytarabine is an antimetabolic agent and DNA synthesis inhibitor with IC50 of 16 nM in wild-type CCRF-CEM cells.

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Cited by 10 Publications

3 Customer Reviews

  • Viability and CI vs Fa after 24-h exposure to cytarabine alone or in combination with ABT-199 in Riva, U2932 and VavP-Bcl2/c-MYC murine tumor cells. Viability shown at 500 nM (500 ng/ml for cytarabine; quadruplicates±s.e.m.).

    Leukemia, 2015, 29(8): 1702–1712. Cytarabine purchased from Selleck.

    Cell cycle was analyzed using propidium iodide (PI) staining and flow cytometry. The plots show PI staining at the x-axis and cell counts at the y-axis. The graphs were prepared using ModFit LT™ software (Verity Software House). Nd: not detected, Ara-c:Cytarabine

    J Exp Clin Cancer Res, 2017, 36(1):22. Cytarabine purchased from Selleck.

  • Flow cytometry analysis for cell surface expression of CD11b shows time- and dose-dependent upregulation of the marker CD11b in MLL-rearranged leukemia cells treated with EPZ-5676 and Cytarabine(Ara-C) as single agents and in combination.

    J Pharmacol Exp Ther, 2014, 350(3): 646-56. Cytarabine purchased from Selleck.

Purity & Quality Control

Choose Selective DNA/RNA Synthesis Inhibitors

Biological Activity

Description Cytarabine is an antimetabolic agent and DNA synthesis inhibitor with IC50 of 16 nM in wild-type CCRF-CEM cells.
Features The 1st of a series of cancer drugs that alters the sugar component of nucleosides.
Targets
DNA synthesis [1]
(CCRF-CEM cells)
16 nM
In vitro

Cytarabine (AraC) is phosphorylated into a triphosphate form (Ara-CTP) involving deoxycytidine kinase (dCK), which competes with dCTP for incorporation into DNA, and then blocks DNA synthesis by inhibiting the function of DNA and RNA polymerases. Cytarabine displays a higher growth inhibitory activity towards wild-type CCRF-CEM cells compared to other acute myelogenous leukemia (AML) cells with IC50 of 16 nM. [1] Increasing concentrations of Cytarabine (IC50 of 0.69 μM) results in decreased metabolic activity of sensitive rat leukemic cell line RO/1, and the cell toxity can be highly enhanced by transfection with human wt dCK (IC50 of 0.037 μM) but not the inactive, alternatively spliced dCK forms. [2] Cytarabine apparently induces apoptosis of rat sympathetic neurons at 10 μM, of which 100 μM shows the highest toxicity and kills over 80% of the neurons by 84 hours, involving the release of mitochondrial cytochrome-c and the activation of caspase-3, and the toxicity can be attenuated by p53 knockdown and delayed by bax deletion. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
mouse L1210 cells MX;DfZRwfG:6aXRCpIF{e2G7 MVuzJIRigXN? MmD2R5l1d3SxeHnjbZR6KGGpYXnud5Qh\GWxeInueYNt\W:|aXTlJIFv[WyxZ4XlMZNmdnOrdHn2[UBud3W|ZTDMNVIyOCClZXzsd{Bi\nSncjCzJIRigXNiYomgUXRVKGG|c3H5MEBKSzVyPUCuOEBvVS5? MW[xPVY4PDlyMx?=
human CCRF-CEM cells MX7DfZRwfG:6aXRCpIF{e2G7 NXro[Gl2OyCmYYnz MkPsR5l1d3SxeHnjbZR6KGGpYXnud5Qh\GWxeInueYNt\W:|aXTlJIFv[WyxZ4XlMZNmdnOrdHn2[UBpfW2jbjDDR3JHNUOHTTDj[YxteyCjZoTldkA{KGSjeYOgZpkhVVSWIHHzd4F6NCCLQ{WwQVEhdk1w NFvMUJgyQTZ5NEmwNy=>
human MCF7 cells NIXKTHBEgXSxdH;4bYPDqGG|c3H5 NILMV3A{KGSjeYO= NYfnXlRLS3m2b4TvfIlkcXS7IHHnZYlve3RiZHXvfJlvfWOuZX;zbYRmKGGwYXzv[5VmNXOnboPpeIl3\SCqdX3hckBOS0Z5IHPlcIx{KGGodHXyJFMh\GG7czDifUBOXFRiYYPzZZktKEmFNUC9OEBvVS5? MYKxPVY4PDlyMx?=
CCRF-CEM cell line MXTGeY5kfGmxbjDhd5NigQ>? NEfFN4NKdmirYnn0JJRp\SC{ZYDsbYNifGmxbjDv[kBES1KILVPFUUBk\WyuIHzpcoUhcW5idnn0do8tKEWGNUC9OUBvVS5? NUSzbXdlOTl7NUi5OC=>
human P12-ICHIKAWA cell MlnSS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MWHJcohq[mm2aX;uJI9nKGi3bXHuJHAyOi2LQ1jJT2FYSSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTVwNUSgcm0v NEPNdFdUSU6JRWK=
human CCRF-CEM cells MnjvR5l1d3SxeHnjxsBie3OjeR?= NVu0WoV{PDhiaB?= MWrDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDDR3JHNUOHTTDj[YxteyCjZoTldkA1QCCqcoOgZpkh[2WubDD0bZRmei2kbIXlJIF{e2G7LDDFR|UxRTVwNkGgcm0v MXSxPVc1Ozh3OB?=
CCRF-CEM cell lines NHjNRWZHfW6ldHnvckBie3OjeR?= NFXXT2U4OiCq MV7Dc45k\W62cnH0bY9vKHKncYXpdoVlKHSxIHnubIljcXRicnXwcIlk[XSrb36gc4YhS0OURj3DSW0h[2WubDDsbY5meyCkeTC1NEUh[W[2ZYKgO|IhcHJiaX7jeYJifGmxbjygSWQ2OD14IH7NMi=> MVqxOlUzODJ2
human CCRF-CEM cells NIm0d4hEgXSxdH;4bYPDqGG|c3H5 M2fxfVQ5KGh? Mk\iR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gR2NTTi2FRV2gZ4VtdHNiYYPz[ZN{\WRiYYOgZ4VtdCC4aXHibYxqfHliYX\0[ZIhPDhiaILzJIJ6KGOnbHz0bZRmei2kbIXlJIF{e2G7LDDJR|UxRTZibl2u NULjT5FVOjJ3OEK5PVE>
human SF268 cells NVj6RYI4S3m2b4TvfIlkyqCjc4PhfS=> NFjuWohEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBUTjJ4ODDj[YxteyCkeTDNWHQh[XO|YYmsJGlEPTB;Nz62PEBvVS5? NXPpeZFOOTl|NEW1PFE>
MOLT-4 human leukemic lymphoblastoid cell lines M1vRcmN6fG:2b4jpZ:Kh[XO|YYm= M13sW2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KE2RTGStOEBpfW2jbjDs[ZVs\W2rYzDsfY1xcG:kbHHzeI9q\CClZXzsJIxqdmW|LDDJR|UxRTFyIH7NMi=> MkjsOlgzPzV2Nh?=
human 697 cell MUnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NVjndWdUUW6qaXLpeIlwdiCxZjDoeY1idiB4OUegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNE43KG6PLh?= NFrlU2NUSU6JRWK=
human ES1 cell NYPIN3VGT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MlzFTY5pcWKrdHnvckBw\iCqdX3hckBGWzFiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zMj65O{BvVS5? NW\JPXlTW0GQR1XS
CCRF-CEM human leukemic lymphoblastoid cell lines NIHl[nNEgXSxdH;4bYPDqGG|c3H5 M1HETWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KEOFUl[tR2VOKGi3bXHuJIxmfWunbXnjJIx6dXCqb3LsZZN1d2mmIHPlcIwhdGmwZYOsJGlEPTB;MkCgcm0v M3jIdFY5Ojd3NE[=
P388 murine cell lines MkHBSpVv[3Srb36gZZN{[Xl? M{GzcVczKGh? NXnwb3RsXGinIHPvcZBwfW6mIIfhd{B1\XO2ZXSgbY4hfmm2cn:g[o9zKGGwdHn0eY1weiCjY4Tpeol1gSh3MDWgbY5pcWKrdHnvckBw\iC2dX3vdkBk\WyuIHfyc5d1cCliYXfhbY5{fCC2aHWgVFM5QCCvdYLpcoUh[2WubDDsbY5meyCjZoTldkA4OiCqb4Xyd{whUUR3ME2yNEBvVS5? M1TqSFM3OjV5MUS=
L1210 murine cell lines M{Dm[GZ2dmO2aX;uJIF{e2G7 NWW4V|k6PzJiaB?= NIDGN|JVcGViY3;tdI92dmRid3HzJJRme3SnZDDpckB3cXS{bzDmc5Ih[W62aYT1cY9zKGGldHn2bZR6MDVyJTDpcohq[mm2aX;uJI9nKHS3bX;yJINmdGxiZ4Lve5RpMSCjZ3HpcpN1KHSqZTDMNVIyOCCvdYLpcoUh[2WubDDsbY5meyCjZoTldkA4OiCqb4Xyd{whUUR3ME2yNEBvVS5? M1XnO|M3OjV5MUS=
human HEL cell M2K4Wmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NWjlUol5UW6qaXLpeIlwdiCxZjDoeY1idiCKRVygZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yNE42OiCwTT6= M3rncnNCVkeHUh?=
human CCRF-CEM/C2 cells NGO0VG1EgXSxdH;4bYPDqGG|c3H5 NUi3cFR1PDhiaB?= M4TETGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGNEWkZvQ1XNM2MzKGOnbHzzJIFnfGW{IES4JIhzeyCkeTDj[YxtKHSrdHXyMYJtfWViYYPzZZktKEWFNUC9NlIvQCCwTT6= Mn;hNVk4PDN6NUi=
mouse L1210 cells NVTQPIlCS3m2b4TvfIlkyqCjc4PhfS=> MYK0PEBp MXHDfZRwfG:6aXPpeJkh[WejaX7zeEBud3W|ZTDMNVIyOCClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJINmdGxicILvcIln\XKjdHnvckBi\nSncjC0PEBpenNuIFnDOVA:OC5yMkSg{txONg>? MkW0NlAxODB2MUi=
human CEM cells NE\ETmREgXSxdH;4bYPDqGG|c3H5 MnL1OFghcA>? MkX6R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gR2VOKGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gZ4VtdCCycn;sbYZmemG2aX;uJIFnfGW{IES4JIhzeyxiSVO1NF0xNjB{NDFOwG0v NWrMSZE{OjByMEC0NVg>
human 2209-23 cells MVTQdo9tcW[ncnH0bY9vKGG|c3H5 NXvWTos5SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjCyNlA6NTJ|IHPlcIx{KGK7IGuzTH11cHmvaXTpcoUhcW6lb4Lwc5JifGmxbjDzZ4lvfGmubHH0bY9vKHC{b4jpcYl1gSCjc4PhfUwhUUN3ME2wMlAzQSEQvF2= MluwNVkxPTV2OEK=
human CHP-212 cell MorzS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MneyTY5pcWKrdHnvckBw\iCqdX3hckBEUFBvMkGyJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N|MvPDdibl2u M3qxPHNCVkeHUh?=
human CTB-1 cell NFzDSVdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M1LSTmlvcGmkaYTpc44hd2ZiaIXtZY4hS1SELUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zOE4{KG6P MWHTRW5ITVJ?
human A427 cell NIrafZRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NVTuUYZvUW6qaXLpeIlwdiCxZjDoeY1idiCDNEK3JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N|QvPDlibl2u M2SwN3NCVkeHUh?=
human MOLT-16 cell NYnhWFJ1T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NGHzc49KdmirYnn0bY9vKG:oIHj1cYFvKE2RTGStNVYh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0{Pi5yNzDuUU4> MljGV2FPT0WU
human MCF7 1K cells M4PwUWN6fG:2b4jpZ:Kh[XO|YYm= MoDCN{Bl[Xm| MkTKR5l1d3SxeHnjbZR6KGGpYXnud5Qh\GWxeInueYNt\W:|aXTlJIFv[WyxZ4XlMZJme2m|dHHueEBpfW2jbjDNR2Y4KDGNIHPlcIx{KG:4ZYLlfJBz\XO|aX7nJJJq[m:wdXPs[Y91cWSnIILl[JVkfGG|ZTDh[pRmeiB|IHThfZMh[nliTWTUJIF{e2G7LDDJR|UxRTRyIH7NMi=> MXexPVY4PDlyMx?=
human MC-IXC cell MoSzS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MlXoTY5pcWKrdHnvckBw\iCqdX3hckBOSy2LWFOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME20NU41OSCwTT6= MXLTRW5ITVJ?
human BHT-101 cell MUHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M1PsOGlvcGmkaYTpc44hd2ZiaIXtZY4hSkiWLUGwNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVQyNjd4IH7N M1\JOXNCVkeHUh?=
human K5 cell NH61O3JIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NWS5bnY6UW6qaXLpeIlwdiCxZjDoeY1idiCNNTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUSzMlIhdk1? NYWwOWtDW0GQR1XS
parent promyelocytic leukemia cell line NInNOotHfW6ldHnvckBie3OjeR?= NEHBU5hCdnSrY3HuZ4VzKGGldHn2bZR6KHejczDleoFtfWG2ZXSg[o9zKHSqZTDjc41xd3WwZDDh[4FqdnO2IIDhdoVvfCCycn;tfYVtd2O7dHnjJIxmfWunbXnhJINmdGxibHnu[UApUExvNkCtS2hRWlRtL3TDT{sqNCCHREWwQVQ4KG6PLh?= NUDs[nNIOzB{N{OyPS=>
human HL60 cells NWf6SIcyS3m2b4TvfIlkyqCjc4PhfS=> MorOR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTGw3OCClZXzsd{Bie3Onc4Pl[EBieyClZXzsJJZq[WKrbHn0fUBi\nSncjC0PEBpenNiYomgZ4VtdHSrdHXyMYJtfWViYYPzZZktKEmFNUC9NE4xPDlizszNMi=> NUPwWWZQOjJ3OEK5PVE>
human A549 cell M2D4U2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NFrUXI9KdmirYnn0bY9vKG:oIHj1cYFvKEF3NEmgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME20PU43PiCwTR?= NHvQSo9USU6JRWK=
human NCI-H1703 cell M2\0dmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NULtTmRNUW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFE4ODNiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD12OT64NUBvVQ>? NInIeGJUSU6JRWK=
L1210 cell lines M1LZfGZ2dmO2aX;uJIF{e2G7 M3LSO2NwdmOnboTyZZRqd25icnXxeYlz\WRidH:gbY5pcWKrdDDy[ZBtcWOjdHnvckBw\iCOMUKxNEBk\WyuIHzpcoV{KGK7IEWwKUBi\nSncjC3NkBpeiCrbnP1ZoF1cW:wLDDFSFUxRTBwMEWg{txONg>? MWWxOlUzODJ2
human K562 cells M{DtXWN6fG:2b4jpZ:Kh[XO|YYm= Ml74R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gT|U3OiClZXzsd{BjgSCVUlKgZZN{[XluIFnDOVA:OC5yNTFOwG0> M{CzRlI1QTV4NUW2
human LB2241-RCC cell MmLWS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M3uyXmlvcGmkaYTpc44hd2ZiaIXtZY4hVEJ{MkSxMXJESyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTV3LkO0JI5O M1PZTXNCVkeHUh?=
human MLMA cell MULHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NH\WOVBKdmirYnn0bY9vKG:oIHj1cYFvKE2OTVGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME21OU42OSCwTR?= NHLPNIdUSU6JRWK=
human P30-OHK cell M2m3UWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M4r5UmlvcGmkaYTpc44hd2ZiaIXtZY4hWDNyLV;IT{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVU3NjVibl2= MkDNV2FPT0WU
RPMI-6410 human leukemic lymphoblastoid cell lines NVHhTIZ4S3m2b4TvfIlkyqCjc4PhfS=> MXzDfZRwfG:6aXPpeJkh[WejaX7zeEBTWE2LLU[0NVAhcHWvYX6gcIV2c2WvaXOgcJlueGixYnzhd5RwcWRiY3XscEBtcW6nczygTWM2OD1yLkC2JO69VQ>? MWC2PFI4PTR4
human HT-29 cells NEi1O3lRem:uaX\ldoF1cW:wIHHzd4F6 NHXwc2FCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjUMVI6KGOnbHzzJIFnfGW{IEm2JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MD6wOlYh|ryPLh?= MojvNlc4QDR2OR?=
human NCI-H510A cell M3f6Zmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NGnhSY1KdmirYnn0bY9vKG:oIHj1cYFvKE6FST3IOVExSSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTZ6LkK2JI5O NXX5SGlsW0GQR1XS
human J-RT3-T3-5 cell M{D6cWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MnXzTY5pcWKrdHnvckBw\iCqdX3hckBLNVKWMz3UN{02KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:PjhwNEKgcm0> NUfSWYliW0GQR1XS
human VA-ES-BJ cell M{[4b2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MWLJcohq[mm2aX;uJI9nKGi3bXHuJHZCNUWVLVLKJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9O|EvPjVibl2= M1PqSHNCVkeHUh?=
human T-24 cell M{C3VWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M37pb2lvcGmkaYTpc44hd2ZiaIXtZY4hXC1{NDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUe4MlU3KG6P NFXSUFdUSU6JRWK=
human NB69 cell NIDSdYRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NGPUb3RKdmirYnn0bY9vKG:oIHj1cYFvKE6ENkmgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME23PU41QCCwTR?= Ml7uV2FPT0WU
human BALL-1 cells NHP2VXlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NG\wToEzPCCq MYLHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDCRWxNNTFiY3XscJMh[W[2ZYKgNlQhcHK|IHL5JHdUXC1zIHHzd4F6NCCJSUWwQVAvODhizszN NH\MPIczOjJ4NEG3NC=>
HL60 cells MWDQdo9tcW[ncnH0bY9vKGG|c3H5 M{n0TWlvKH[rdILvJIlvcGmkaYTvdpkh[2:wY3XueJJifGmxbjDh[4FqdnO2IIDyc4xq\mW{YYTpc44hd2ZiSFy2NEBk\WyuczygTWM2OD1yLkC4JO69VQ>? MmroNVI5Pzd3OUO=
human CTV-1 cell M3;VS2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MVjJcohq[mm2aX;uJI9nKGi3bXHuJGNVXi1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;OESuO|Qhdk1? NIrKclZUSU6JRWK=
human MEL-JUSO cell M4HI[mdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1jhemlvcGmkaYTpc44hd2ZiaIXtZY4hVUWOLVrVV28h[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF05PS55NjDuUS=> NYS0W5VHW0GQR1XS
human MOLT-4 cell NUnEWWpyT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NUTibXIyUW6qaXLpeIlwdiCxZjDoeY1idiCPT1zUMVQh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF05QS54NzDuUS=> MYHTRW5ITVJ?
human leukemia cell line(CCRF-CEM) NFn4fnpEgXSxdH;4bYPDqGG|c3H5 M4XsO2lvKH[rdILvJIN6fG:2b4jpZ4l1gSCxZjDjc41xd3WwZIOge4Vz\SCmZYTldo1qdmWmIH;uJGlvcGmkaYTpc44hd2ZiaIXtZY4hdGW3a3XtbYEh[2WubDDsbY5mMEOFUl[tR2VOMSxiSVO1NF0xNjB7IN88UU4> MljYPFkyPzZ2NR?=
human SW982 cell M3\6T2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Ml[3TY5pcWKrdHnvckBw\iCqdX3hckBUXzl6MjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUmxMlI{KG6P M{P5dnNCVkeHUh?=
human NCI-SNU-1 cell NVfocm5NT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NFfocpBKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3TUnUuOSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G778{MJGlEPTB;OU[uO|chdk1? NXPhWWpvW0GQR1XS
HeLa human cell lines MU\GeY5kfGmxbjDhd5NigQ>? M1PncFE1PCCq NWnrZZhHXGinIHPvcZBwfW6mIIfhd{B1\XO2ZXSgbY4hfmm2cn:g[o9zKGGwdHn0eY1weiCjY4Tpeol1gSh3MDWgbY5pcWKrdHnvckBw\iC2dX3vdkBk\WyuIHfyc5d1cCliYXfhbY5{fCCKZVzhJIh2dWGwIHPlcIwhdGmwZYOgZYZ1\XJiMUS0JIhzNCCLREWwQVAvOSEQvF2= MUWzOlI2PzF2
human H460 cells MVfGeY5kfGmxbjDhd5NigQ>? NIDvOJgzPCCq NED4fppCdnSrdIXtc5Ih[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIOFYxKGOnbHzzJIFnfGW{IEK0JIhzeyCkeTDNWHQh[XO|YYpCpC=> MV2yNFIyOTV4NB?=
human HCT116 cells M3r5bGZ2dmO2aX;uJIF{e2G7 MkXCRY51cXS3bX;yJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSFPUNVE3KGOnbHzzJIFnfGW{IEK0JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MD6xJO69VQ>? MnHlNlAzOTF3NkS=
human GP5d cell M33yXWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NXnpXJRrUW6qaXLpeIlwdiCxZjDoeY1idiCJUEXkJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVAzKG6P NWS0[Vh{W0GQR1XS
human SAS cell NXnESW42T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M1\RbWlvcGmkaYTpc44hd2ZiaIXtZY4hW0GVIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUC3MlU4KG6P M3m1d3NCVkeHUh?=
human BFTC-905 cell  NHLBU2NIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MUTJcohq[mm2aX;uJI9nKGi3bXHuJGJHXENvOUC1JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVA5NjNzIH7N NUDzdFNnW0GQR1XS
human LB1047-RCC cell MVrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NYe0ZoFiUW6qaXLpeIlwdiCxZjDoeY1idiCOQkGwOFcuWkOFIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUC4MlQzKG6P M13PcnNCVkeHUh?=
human BB65-RCC cell NHW0V3NIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MkjLTY5pcWKrdHnvckBw\iCqdX3hckBDSjZ3LWLDR{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEyOC5zOTDuUS=> NVGyboJPW0GQR1XS
human KE-37 cell NFTQWXpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NUPBW4JrUW6qaXLpeIlwdiCxZjDoeY1idiCNRT2zO{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEyOC56NTDuUS=> M{HZVHNCVkeHUh?=
human NCI-H292 cell MV7Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NUTpXVdGUW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFI6OiClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTFzND6yJI5O NUL6WGVVW0GQR1XS
human HT-1080 cell MV3Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MoLSTY5pcWKrdHnvckBw\iCqdX3hckBJXC1zMEiwJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVE1NjR5IH7N NHKwSnhUSU6JRWK=
human HL60 cells MnXrR5l1d3SxeHnjxsBie3OjeR?= M4DlR|Q5KGh? MXLDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIUFYxKGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MD6xNVgh|ryP MYqxPVMzOTJ|NB?=
human CAKI-1 cell MVHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MnPoS5Jwf3SqIHnubIljcXSrb36gc4Yh[WejaX7zeEBpfW2jbjDIR3QyOTZiY3XscJMh[W[2ZYKgNlQhcHK|IHL5JHdUXC1zIHHzd4F6NCCJSUWwQVAvOTJizszN NXTX[WJpOjJ{NkSxO|A>
human CAKI-1 cell NWrJN3FwT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MWLJcohq[mm2aX;uJI9nKGi3bXHuJGNCU0lvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGyNEBvVQ>? MWDTRW5ITVJ?
human HMV-II cell NF7wU4FIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MX;Jcohq[mm2aX;uJI9nKGi3bXHuJGhOXi2LSTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5 MU\TRW5ITVJ?
human ES8 cell M4LjfWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MXXJcohq[mm2aX;uJI9nKGi3bXHuJGVUQCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTF{Mz65N{BvVQ>? NEHz[3BUSU6JRWK=
human NEC8 cell Mon6S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NXLGSYE3UW6qaXLpeIlwdiCxZjDoeY1idiCQRVO4JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVM1NjJzIH7N MmfQV2FPT0WU
human EW-1 cell Ml;4S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MknyTY5pcWKrdHnvckBw\iCqdX3hckBGXy1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUO4MlYzKG6P NXTNO5JIW0GQR1XS
murine leukemia P388 M1LTWmZ2dmO2aX;uJIF{e2G7 NF:xfpNCdnSrY3HuZ4VzKGGldHn2bZR6KGOjcoLp[YQhd3W2IHnuJJZqfHKxIHHnZYlve3RibYXybY5mKGyndXvlcYliKFB|OEisJGlEPTB;MD6xOEDPxE1? NGfr[4IyOTN3NkGxNC=>
human H-EMC-SS cell NFf4N2JIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MkDtTY5pcWKrdHnvckBw\iCqdX3hckBJNUWPQz3TV{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE1PS5{ODDuUS=> NGniWmNUSU6JRWK=
human SNU638 cells MUjDfZRwfG:6aXRCpIF{e2G7 NEfRUXBEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBUVlV4M{igZ4VtdHNiYomgV3JDKGG|c3H5MEBKSzVyPUCuNVUh|ryP MUiyOFk2PjV3Nh?=
human COR-L105 cell M1fsfGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1vRV2lvcGmkaYTpc44hd2ZiaIXtZY4hS0:ULVyxNFUh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yPjBwM{mgcm0> NETvc2VUSU6JRWK=

... Click to View More Cell Line Experimental Data

In vivo Cytarabine is highly effective against acute leukaemias, which causes the characteristic G1/S blockage and synchronization, and increases the survival time for leukaemic Brown Norway rats in a weak dose-related fashion indicating that the use of higher dosages of Cytarabine does not contribute to its antileukaemic effectiveness in man. [4] Cytarabine (250 mg/kg) also causes placental growth retardation and increases placental trophoblastic cells apoptosis in the placental labyrinth zone of the pregnant Slc:Wistar rats, which increases from 3 hour after the treatment and peaks at 6 hour before returning to control levels at 48 hour, with remarkably enhanced p53 protein, p53 trancriptional target genes such as p21, cyclinG1 and fas and caspase-3 activity. [5]

Protocol

Kinase Assay:[1]
+ Expand

In Vitro Growth Inhibition Assay:

Stock solution of Cytarabine is prepared in absolute ethanol, and serial dilutions of Cytarabine are prepared. CCRF-CEM cells are suspended in RPMI medium supplemented with 10% FBS, 0.1% gentamicin, and 1% sodium pyruvate. The cells are suspended in their respective media to give 10 mL volumes of cell suspension at a final density of 3-6 × 104 cells/mL. Appropriate volumes of Cytarabine solution are transferred to the cell suspensions, and incubation is continued for 72 hours. The cells are spun down and resuspended in fresh Cytarabine -free medium, and final cell counts are determined. The data are analyzed by sigmoidal curve fitting of the cell count versus Cytarabine concentration, and the results are expressed as the IC50 (Cytarabine concentration that inhibits cell growth to 50% of the control value).
Cell Research:[2]
+ Expand
  • Cell lines: Rat leukemic cell lines RCL/0, RO/1 and K7 and human myelomonocytic leukemic U937
  • Concentrations: ~100 μM
  • Incubation Time: 24, 48 and 72 hours
  • Method: Cells are incubated in the presence of different concentrations of Cytarabine at 37 °C for 24, 48, and 72 hours. At the time of 20-, 44-, or 68-hour incubation in the presence of Cytarabine, 10 mL of cell proliferation reagent WST-1 solution is added. After 2- and 4-hour incubation with WST-1, cell metabolic activity is assessed with colorimetric changes quantified by measuring the absorbance in a spectrophotometer at 450 nm. And cell division times are calculated from eosin counting in parallel with viability assay
    (Only for Reference)
Animal Research:[4]
+ Expand
  • Animal Models: Brown Norway rat with myelocytic leukaemia
  • Formulation: Dissolved in phosphate-buffered saline (pH 7.0) just before use.
  • Dosages: 5 - 1000 mg/kg
  • Administration: Injection i.v.
    (Only for Reference)

Solubility (25°C)

In vitro Water 48 mg/mL (197.35 mM)
DMSO 1 mg/mL (4.11 mM)
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
Saline
For best results, use promptly after mixing.
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 243.22
Formula

C9H13N3O5

CAS No. 147-94-4
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
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    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
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Molecular Weight Calculator

Molecular Weight Calculator

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Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

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Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

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Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03571321 Not yet recruiting Acute Lymphoblastic Leukemia|ALL Childhood|ALL University of Chicago|Incyte Corporation September 5 2019 Phase 1
NCT03571321 Not yet recruiting Acute Lymphoblastic Leukemia|ALL Childhood|ALL University of Chicago|Incyte Corporation September 5 2019 Phase 1
NCT03719560 Not yet recruiting Diffuse Large B Cell Lymphoma Brown University July 1 2019 Phase 2
NCT03836209 Not yet recruiting Acute Myeloid Leukemia PrECOG LLC.|Astellas Pharma Inc July 2019 Phase 2
NCT03719560 Not yet recruiting Diffuse Large B Cell Lymphoma Brown University July 1 2019 Phase 2
NCT03836209 Not yet recruiting Acute Myeloid Leukemia PrECOG LLC.|Astellas Pharma Inc July 2019 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID