Name: Cidofovir
Brand: Selleck Chemicals
SKU: S1516
Rating: 5 (8 reviews)

Cidofovir suppresses virus replication by selective inhibition of viral DNA synthesis.

Cidofovir Chemical Structure

CAS: 113852-37-2

Selleck's Cidofovir has been cited by 8 Publications

2 Customer Reviews

Purity & Quality Control

Batch: Purity: 99.97%

99.97

Cidofovir Related Products

Related Targets	tRNA synthetase RdRp DNA synthesis helicase ribonucleotide reductase	Click to Expand
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Related Compound Libraries	FDA-approved Drug Library Natural Product Library Apoptosis Compound Library DNA Damage/DNA Repair compound Library Cell Cycle compound library	Click to Expand

Signaling Pathway

DNA/RNA Synthesis Signaling Pathway

Choose Selective DNA/RNA Synthesis Inhibitors

Cell Data

Cell Lines	Assay Type	Incubation Time	Activity Description	PMID
HEL cells	Function assay		Compound was tested for anti-viral activity against HSV-1(KOS) in HEL cells, EC50=0.57 μM	14643328
MRC-5 cells	Function assay		Inhibitory activity against cytopathic effect of HSV-2(E 194) in MRC-5 cells, IC50=10 μM	8632439
NHDF cells	Function assay		Inhibitory activity against replication of HCMV in NHDF cells, IC50=0.5 μM	8632439
HFF cells	Function assay		Antiviral activity against Vaccinia virus Copenhagen in HFF cells assessed as reduction in cytopathogenicity, EC50=3.2 μM	16603351
HFF cells	Function assay		Antiviral activity against Cowpox virus Brighton in HFF cells assessed as reduction in cytopathogenicity, EC50=7.1 μM	16603351
HFF cells	Function assay		Antiviral activity against vaccinia virus Copenhagen measured as cytopathogenicity in HFF cells, EC50=6.9 μM	16722657
bone marrow cells	Cytotoxicity assay		Cytotoxicity against human bone marrow cells assessed as inhibition of colony forming unit of granulocyte/macrophage, IC50=10 μM	16814545
HFF cells	Function assay		Antiviral activity against Human CMV T2241 in HFF cells by SEAP assay, IC50=0.3 μM	17043128
MRC5 cells	Function assay		Antiviral activity against Human CMV Towne in MRC5 cells by PRA, IC50=0.3 μM	17043128
UC1B cells	Function assay		Antiviral activity against Murine polyomavirus MN/RDE Toronto in mouse UC1B cells assessed as reduction of virus-induced cytopathogenicity, EC50=13 μM	17420214
african green monkey Vero cells	Function assay		Antiviral activity against Herpes simplex virus 1 F infected in african green monkey Vero cells assessed as plaque reduction after 36 to 48 hrs, EC50=14.4 μM	17438061
HEL cells	Function assay	3 days	Antiviral activity against HCMV AD169 infected in HEL cells assessed as reduction of virus-induced cytopathogenicity after 3 days, EC50=0.41 μM	17961851
HEL cells	Function assay	3 days	Antiviral activity against HCMV Davis infected in HEL cells assessed as reduction of virus-induced cytopathogenicity after 3 days, EC50=0.41 μM	17961851
HFF cells	Function assay		Antiviral activity against Cytomegalovirus CMV T2211 infected in HFF cells by SEAP reporter gene assay, EC50=0.22 μM	17709468
HEL cells	Function assay		Antiviral activity against ganciclovir-resistant HCMV AD169 clone 4 infected in HEL cells assessed as inhibition of virus-induced cytopathicity, EC50=0.74 μM	19226140
HEL cells	Function assay		Antiviral activity against HCMV Davis infected in human HEL cells assessed inhibition of virus-induced cytopathicity after 7 days postinfection, EC50=0.5 μM	19226140
HEL cells	Function assay		Antiviral activity against HCMV AD169 infected in human HEL cells assessed inhibition of virus-induced cytopathicity after 7 days postinfection, EC50=0.3 μM	19226140
HEL cells	Function assay		Antiviral activity against foscarnet-resistant HCMV AD169 clone C infected in HEL cells assessed as inhibition of virus-induced cytopathicity, IC50=0.045 μM	19226140
HFF	Proliferation assay	3 days	Antiproliferative activity against HFF after 3 days by coulter counter assay, EC50=1.9 μM	19029322
HeLaS3 cells	Function assay	3-5 days	Antiviral activity against Vaccinia virus IHD-J ATCC VR-156 infected in HeLaS3 cells after 3 to 5 days by plaque assay, EC50=18.74 μM	18852271
HSB2 cells	Function assay	7 days	Antiviral activity against Human herpesvirus 6 GS infected in human HSB2 cells assessed as decrease in viral DNA accumulation after 7 days, EC50=2.7 μM	19770274
A549 cells	Function assay		Antiviral activity against Human adenovirus type 11p slobitski infected in A549 cells assessed as inhibition of DNA replication by QPCR assay, EC50=16.5 μM	20585112
HFF cells	Function assay		Antiviral activity against Cowpox virus (Brighton Red) infected in human HFF cells assessed as reduction in viral-induced cytopathic effect by neutral red uptake assay, EC50=6.7 μM	21376429
Click to View More Cell Line Experimental Data

Biological Activity

Description	Cidofovir suppresses virus replication by selective inhibition of viral DNA synthesis.

In vitro
In vitro	Cidofovir inhibits human cytomegalovirus (HCMV) infection in cultured cells. Cidofovir is inhibitory to CMV plaque formation even when added to the cells at 48 hr post infection with IC50 of 0.9 μg/mL for Davis and 1.6 μg/mL for AD-169 strains,respectively. ^[1] Cidofovir also inhibits herpes simplex virus infection. In addition, Cidofovir blocks cell fusion induced by HSV-1 in monkey kidney cells and blocks the expression of HSV-l-specific proteins and the synthesis of viral DNA. ^[3]

In Vivo
In vivo	Cidofovir (5 mg/kg/day) subcutaneously for 5 days significantly reduces average virus infectivity titer in blood, spleen, lung and salivary gland in infected guinea pigs. Cidofovir significantly reduces lymphocytosis and average tissue indexe of spleen in infected animals. ^[2]. Cidofovir suppresses all manifestations (skin lesions, paralysis of the hind legs, and mortality) of hairless mice infected intracutaneously with HSV-1 or HSV-2. The most remarkable feature of Cidofovir is that a single administration of the compound, even as late as 4 days after infection, conferees significant protection against HSV-1 or HSV-2 infection. ^[4] Cidofovir inhibits growth of the highly aggressive melanoma tumor arising from mouse melanoma B16 cells grafted subcutaneously in C57B16/J mice. ^[5]

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-01-11)
NCT04542252	Completed	Drug Drug Interaction	SymBio Pharmaceuticals	November 9 2020	Phase 1
NCT01610765	Withdrawn	Herpes Simplex Virus	University of Alabama at Birmingham	January 2016	Phase 1\|Phase 2
NCT01816646	Completed	Blood And Marrow Transplantation	M.D. Anderson Cancer Center\|Gilead Sciences	September 2013	Phase 1
NCT00780182	Completed	Healthy	Chimerix\|National Institutes of Health (NIH)	October 2008	Phase 1

References

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Chemical Information & Solubility

Molecular Weight	279.19	Formula	C₈H₁₄N₃O₆P
CAS No.	113852-37-2	SDF	Download Cidofovir SDF
Smiles	C1=CN(C(=O)N=C1N)CC(CO)OCP(=O)(O)O
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vitro
Batch:

Water : 3 mg/mL

DMSO : Insoluble ( Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : Insoluble

Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.

In vivo Formulation Calculator

Preparing Stock Solutions

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Dilution Calculator Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

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% DMSO + % + % Tween 80 + % ddH₂O

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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