4-PBA (4-Phenylbutyric acid)

Catalog No.S3592 Batch:S359201

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Technical Data

Formula

C10H12O2
Molecular Weight 164.20 CAS No. 1821-12-1
Solubility (25°C)* In vitro DMSO 33 mg/mL (200.97 mM)
Ethanol 33 mg/mL (200.97 mM)
Water Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
4.0mg/ml Taking the 1 mL working solution as an example, add 50 μL of 80 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description 4-PBA (4-Phenylbutyric acid) is a histone deacetylase (HDAC) inhibitor and a key epigenetic inducer of anti-HCV hepatic hepcidin. 4-Phenylbutyric acid inhibits LPS-induced inflammation through regulating endoplasmic-reticulum (ER) stress and autophagy in acute lung injury models.
Targets
HDAC [1]
In vitro

4-PBA inhibits the ER stress induced by SiNPs in RAW264.7 cells, as evidenced by the expansion and degranulation of ER, as well as greatly up-regulated Bip and CHOP expressions.[3]
In vivo

4-PBA attenuates LPS-induced bone loss in lipopolysaccharide (LPS)-treated mice, by increasing area of TRAP-positive osteoclasts (OCs) and serum level of collagen type I fragments.[4]

Protocol (from reference)

Cell Assay:

[3]
  • Cell lines

    Mouse macrophage cell line, RAW264.7 cells

  • Concentrations

    3 mM

  • Incubation Time

    6 h

  • Method

    Mouse macrophage cell line, RAW264.7 cells are cultured in DMEM with 10% fetal bovine serum at 37 ℃ in a 5% CO2 incubator. SiNPs are diluted by DMEM to appropriate concentrations, and an ER stress inhibitor, 4-phenylbutyric acid (4-PBA) is applied (3 mM, 6 h). The SiNPs-treated group is at a concentration of 50 μg/ml in vitro experiments. Similarly, the application of 4-PBA is set up through MTT assay, after SiNPs with or without 4-PBA treatment, cells are harvested for the following measurement. The levels of Bip and CHOP induced by SiNPs are measured.

Selleck's 4-PBA (4-Phenylbutyric acid) has been cited by 14 publications

A novel CSN5/CRT O-GlcNAc/ER stress regulatory axis in platinum resistance of epithelial ovarian cancer [ Int J Biol Sci, 2024, 20(4):1279-1296] PubMed: 38385070
Inhibition of OGFOD1 by FG4592 confers neuroprotection by activating unfolded protein response and autophagy after ischemic stroke [ J Transl Med, 2024, 22(1):248] PubMed: 38454480
IRE1α arm of unfolded protein response in muscle-specific TGF-β signaling-mediated regulation of muscle cell immunological properties [ Cell Mol Biol Lett, 2023, 28(1):15] PubMed: 36849929
Porcine reproductive and respiratory syndrome virus infection triggers autophagy via ER stress-induced calcium signaling to facilitate virus replication [ PLoS Pathog, 2023, 19(3):e1011295] PubMed: 36972295
Routes of Albumin Overload Toxicity in Renal Tubular Epithelial Cells [ Int J Mol Sci, 2023, 24(11)9640] PubMed: 37298591
Large HBV Surface Protein-Induced Unfolded Protein Response Dynamically Regulates p27 Degradation in Hepatocellular Carcinoma Progression [ Int J Mol Sci, 2023, 10.3390/ijms241813825] PubMed: 37762128
Endoplasmic reticulum stress mediates environmental particle-induced inflammatory response in bronchial epithelium [ J Immunotoxicol, 2023, 10.1080/1547691X.2023.2229428] PubMed: 37417782
Effects of endoplasmic reticulum stress on erectile function in rats with cavernous nerve injury [ Sex Med, 2023, 11(4):qfad050] PubMed: 37674767
ASK1-ER stress pathway-mediated fibrotic-EV release contributes to the interaction of alveolar epithelial cells and lung fibroblasts to promote mechanical ventilation-induced pulmonary fibrosis [ Exp Mol Med, 2022, 1-13.] PubMed: 36473935
A heterozygous p.S143P mutation in LMNA associates with proteasome dysfunction and enhanced autophagy-mediated degradation of mutant lamins A and C [ Front Cell Dev Biol, 2022, 10:932983] PubMed: 36111332

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.