3-Nitropropionic acid

Catalog No.S3652 Synonyms: β-Nitropropionic Acid, 3-NP

For research use only.

3-Nitropropionic acid (β-Nitropropionic Acid, 3-NP) is an irreversible inhibitor of mitochondrial respiratory Complex II succinate dehydrogenase, resulting in energy depletion through disruption of the electron transport chain.

3-Nitropropionic acid Chemical Structure

CAS No. 504-88-1

Selleck's 3-Nitropropionic acid has been cited by 1 Publication

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Biological Activity

Description 3-Nitropropionic acid (β-Nitropropionic Acid, 3-NP) is an irreversible inhibitor of mitochondrial respiratory Complex II succinate dehydrogenase, resulting in energy depletion through disruption of the electron transport chain.
Targets
Succinate dehydrogenase [1]
()
In vitro

3-nitropropionic acid (3-NPA) is an irreversible inhibitor of complex II in the mitochondria. It impairs cellular energy metabolism via inhibition of succinate dehydrogenase, which induces a reduction in ATP production and leads to oxidative stress. 3-NPA can cause the generation and release of ROS from mitochondria, mitochondrial DNA damage, and thus loss of mitochondrial function. 3-NPA reduces the number of large follicles, impairs oocyte development, and increases the percentage of atretic large follicles and ROS levels in oocytes and granulosa cells[2]. ROS production followed by mitochondrial DNA damage is the primary event in 3-NPA toxicity, and Bcl-2 protects PC12 cells from 3-NPA toxicity by preventing mitochondrial DNA damage[3].

In vivo 3-NPA induces granulosa cell apoptosis, large follicle atresia, and an increase of ROS levels in the ovary[2].

Protocol (from reference)

Cell Research:[3]
  • Cell lines: PC12 cells
  • Concentrations: 4-8 mM
  • Incubation Time: 48 h
  • Method: Viability of the PC12 cells with or without treatment of 3-NPA is estimated using trypan blue exclusion test. Cells are grown in Petri dishes to a density of 1 × 106 cells/dish. After treatment with 4 or 8 mM 3-NPA for 48 h, cells are washed with Hank's balanced salt solution. Trypan blue (0.4 %) is added to the cells and incubated for 5 min. Both viable and nonviable cells are scored on a hemocytometer using a Nikon microscope. The nonviable cells are expressed as a percentage of total cell population
  • (Only for Reference)
Animal Research:[2]
  • Animal Models: ICR mice
  • Dosages: 6.25, 12.5 and 25 mg/kg
  • Administration: i.p.
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 23 mg/mL
(193.14 mM)
Water 23 mg/mL
(193.14 mM)
Ethanol 23 mg/mL
(193.14 mM)

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 119.08
Formula

C3H5NO4

CAS No. 504-88-1
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C(C[N+](=O)[O-])C(=O)O

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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