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2-DG (2-Deoxy-D-glucose) Glycolysis inhibitor

Cat.No.S4701

2-DG (2-Deoxy-D-glucose), an analog of glucose, is a glycolytic inhibitor with antiviral activity. 2-Deoxy-D-glucose induces apoptosis and inhibits Herpes Simplex Virus type-1 (HSV-1) receptor expression.

2-DG (2-Deoxy-D-glucose) Carbohydrate Metabolism modulator Chemical Structure

Chemical Structure

Molecular Weight: 164.16

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Quality Control

Batch: Purity: >97%

Cited in Nature Medicine for its top-tier quality
COA
NMR
Datasheet
SDS/MSDS

Cited in Nature Medicine for its top-tier quality
COA
NMR
Datasheet
SDS/MSDS

Cited in Nature Medicine for its top-tier quality
COA
NMR
Datasheet
SDS/MSDS

Cited in Nature Medicine for its top-tier quality
COA
NMR
Datasheet
SDS/MSDS

Cited in Nature Medicine for its top-tier quality
COA
NMR
Datasheet
SDS/MSDS

97

Related Targets	Dehydrogenase HSP Transferase P450 (e.g. CYP17) PDE phosphatase PPAR Vitamin Mitochondrial Metabolism Casein Kinase
Other Carbohydrate Metabolism Inhibitors	Bromopyruvic acid (3-BP) Dorzagliatin 1-Deoxynojirimycin LY2608204 Voglibose AZD1656 4',7-Dimethoxy-5-Hydroxyflavone BI-9787 Eleutherol Kaempferol-3-O-neohesperidoside

Q&A of 2-DG (2-Deoxy-D-glucose): Insights Rooted in Extensive Publications, Every Answer Cited

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Solubility in DMSO or Water

In vitro
Batch:

DMSO : 500 mg/mL (3045.8 mM) Warmed with 60°C water bath; Ultrasonicated;
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : 83 mg/mL

Ethanol : 8 mg/mL

The solubility data above are all experimental results (not literature values).

DMSO : 33 mg/mL (201.02 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : 33 mg/mL

Ethanol : 5.5 mg/mL

The solubility data above are all experimental results (not literature values).

DMSO : 33 mg/mL (201.02 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : 33 mg/mL

Ethanol : 8 mg/mL

The solubility data above are all experimental results (not literature values).

Water : 100 mg/mL

DMSO : 33 mg/mL (201.02 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 5 mg/mL

The solubility data above are all experimental results (not literature values).

DMSO : 33 mg/mL (201.02 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : 33 mg/mL

Ethanol : Insoluble

The solubility data above are all experimental results (not literature values).

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	1.670mg/ml (10.17mM)	Taking the 1 mL working solution as an example, add 50 μL of 33.3 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to make it clear. Volume up to 1 mL. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

%

% Tween 80

% ddH₂O

% DMSO

+

%

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Read more about 2-DG (2-Deoxy-D-glucose) solubility in DMSO or water

Search directly for a cell line, or select a cell line to find matched working concentrations

Read more about 2-DG (2-Deoxy-D-glucose) working concentrations for cell culture treatment

2-DG (2-Deoxy-D-glucose) is a potent inhibitor of WNV (500 mg/kg), cellular ATP (10 mM), WNV-induced neuroinflammation (500 mg/kg), immune cells (500 mg/kg), Isg15 (500 mg/kg), Usp18 (500 mg/kg), Mx2 (500 mg/kg), interleukins signaling (500 mg/kg), CCL2 (500 mg/kg), CXCL10 (500 mg/kg), CXCL11 (500 mg/kg), TNF-α (500 mg/kg), HK, GLUT, HK2, HIF-1α, AMPK, GPI, glycolytic pathway, glycolysis, glucose metabolism, ATP production, ROS, Beclina-1, Atg-3, FRAP, mitochondrial complex I, mitochondrial complex IV, CAT, SOD, viral synthesis, protein energy production, glycosylation, mTOR, TSC1, SARS-CoV-2, N-glycosylation, UPR pathway, viral replication, glycosylation of viral proteins, inflammatory responses, hyperglycolysis, metabolic reprograming, CD8+ T cell-mediated cytotoxicity, IFN-g production, MHCII, CD86, TNFα production, WNV multiplication, GLUT1, cellular viability, clonogenic survival, apoptotic cell, angiogenesis, cell viability, proliferation, N-linked glycosylation, cell proliferation, IL-1B, aerobic glycolysis, PI3K signaling, mTOR signaling, GLUTs, cellular ATP production, Akt phosphorylation, endoplasmic reticulum stress, autophagy, tumor burden, tumor growth, oxygen consumption rate, mitochondrial content, PDH, LDH, mTORC1, S6K1, ACC, AMPK phosphorylation, mTORC1-mediated promotion of glycolysis, ERK phosphorylation, PI3K/AKT, ATP-dependent drug efflux transporters. 2-DG (2-Deoxy-D-glucose) exhibits the greatest inhibitory potency toward cellular ATP (IC50 = 10000000 nM).

Read more about selectivity & specificity

Mechanism of Action

Information	2-Deoxy-D-glucose (2-DG) is a competitive hexokinase inhibitor. By blocking glycolysis and depleting cellular ATP, it activates the AMPK pathway while suppressing PI3K/AKT/mTOR signaling, and modulates HIF-1α activity, thereby inhibiting tumor cell proliferation and migration, and promoting apoptosis.
Read more about 2-DG (2-Deoxy-D-glucose) IC50 for cell-based and cell-free assays
Primary Applications and Mechanism of Action

Read more about 2-DG (2-Deoxy-D-glucose) Mechanism of Action

Chemical Information, Storage & Stability

Molecular Weight	164.16	Formula	C₆H₁₂O₅	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	154-17-6	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	2-deoxyglucose, NSC 15193, 2-Deoxy-D-arabino-hexose, D-Arabino-2-deoxyhexose			Smiles	C(C=O)C(C(C(CO)O)O)O

Read more about storage & stability

2-DG (2-Deoxy-D-glucose) Combination Database

Read more comparative analysis about 2-DG (2-Deoxy-D-glucose)

Tech Support

Handling Instructions

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Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration _(start) x Volume _(start) = Concentration _(final) x Volume _(final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

Serial Dilutions

Concentration (start):

Dilution-folds:

Computed Result

C1=C0/X	C1:		LOG(C1):
C2=C1/X	C2:		LOG(C2):
C3=C2/X	C3:		LOG(C3):
C4=C3/X	C4:		LOG(C4):
C5=C4/X	C5:		LOG(C5):
C6=C5/X	C6:		LOG(C6):
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C8=C7/X	C8:		LOG(C8):