Catalog No.S3839 Synonyms: duvoglustat, moranolin

For research use only.

1-Deoxynojirimycin (duvoglustat, moranolin) is a potent α-glucosidase inhibitor and most commonly found in mulberry leaves. It has therapeutic potency against diabetes mellitus.

1-Deoxynojirimycin Chemical Structure

CAS No. 19130-96-2

Purity & Quality Control

Choose Selective Carbohydrate Metabolism Inhibitors

Biological Activity

Description 1-Deoxynojirimycin (duvoglustat, moranolin) is a potent α-glucosidase inhibitor and most commonly found in mulberry leaves. It has therapeutic potency against diabetes mellitus.
α-glucosidase [3]
(Cell-free assay)
In vitro

1-Deoxynojirimycin (DNJ) up to 200 μmol/L does not influence survival of HUVECs. DNJ behaves as an antioxidant, decreases ROS production, and delays cellular senescence[2].

In vivo 1-Deoxynojirimycin (DNJ) inhibits intestinal glucose absorption in intestine. 1-Deoxynojirimycin down-regulates intestinal SGLT1, Na+/K+-ATP and GLUT2 mRNA and protein expression. Pretreatment with DNJ (50 mg/kg) increases the activity, mRNA and protein levels of hepatic glycolysis enzymes (GK, PFK, PK, PDE1) and decreases the expression of gluconeogenesis enzymes (PEPCK, G-6-Pase), improves glucose tolerance in both normal and diabetic mice. DNJ inhibits intestinal glucose absorption and accelerates hepatic glucose metabolism by directly regulating the expression of proteins involved in glucose transport systems, glycolysis and gluconeogenesis enzymes[1]. DNJ reduces oxidative stress in the liver and plasma in rodents[2].

Protocol (from reference)

Cell Research:


  • Cell lines: HUVECs
  • Concentrations: 0-200 μmol/L
  • Incubation Time: 24 or 48 h
  • Method:

    Cytotoxicity of DNJ is assessed using a WST-8 assay. HUVECs at 80% confluency are trypsinized and transferred to 96-well plates (4000 cells/well). Stock solutions of DNJ are prepared in HuMedia-EG2 medium. DNJ test media are prepared from the stock solutions and diluted to final concentrations of 0-200 μmol/L in HuMedia-EG2 medium. After incubation for 24 h at 37℃, the cells are placed in 200 μL of fresh HuMedia-EG2 medium with various concentrations of DNJ. After 24 or 48 h, 10 μL WST-8 solution is added to each well. After incubation for 3 h at 37℃, cytotoxicity is measured using a microplate reader at a wavelength of 450 nm and a reference wavelength of 655 nm.

Animal Research:


  • Animal Models: ICR mice
  • Dosages: 50 mg/kg
  • Administration: administered intragastrically

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 163.17


CAS No. 19130-96-2
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1C(C(C(C(N1)CO)O)O)O

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Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT01380743 Completed Drug: duvoglustat|Drug: rhGAA Pompe Disease Amicus Therapeutics October 31 2011 Phase 2

(data from https://clinicaltrials.gov, updated on 2022-08-01)

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