SN-38

Catalog No.S4908

SN-38 Chemical Structure

Molecular Weight(MW): 392.4

SN-38 is an active metabolite of CPT-11, inhibits DNA topoisomerase I, DNA synthesis and causes frequent DNA single-strand breaks.

Size Price Stock Quantity  
USD 90 In stock
USD 370 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

2 Customer Reviews

  • (B) HCT116 cells were treated with increasing doses of SN-38 and treated with 4 nM SN-38 for different time. Cell extracts were prepared and analyzed by Western blotting with indicated antibody. These experiments were repeated thrice. (C) HCT116 cells were transfected with 2 μg of EGFP-LC3 construct. At 8 h post-transfection, cells were treated with 4 nM of SN-38 for 48 h. And then cells were examined by confocal microscopy (magnification × 400). The percentage of cells showing accumulation of EGFP-LC3 in puncta (EGFP-LC3vac) was quantified. (D) LOVO and HCT116 cells were treated with 2 nM and 4 nM of SN-38 combined with 10 mM of 3-Methyladenine (3-MA) for 48 h respectively. Cell extracts were prepared and analyzed by Western blotting with indicated antibody. These experiments were repeated thrice. (E) Cells were treated with indicated concentrations of 3-MA and SN-38 for 48 h. Cell apoptosis was assessed by Annexin V-FITC/PI staining assay by flow cytometry. Columns, means of three determinations; bars, SD. (F) and (G) LOVO and HCT116 cells were transfected with 50 nM of NC siRNA, ATG5 siRNA respectively, and then were treated with increasing doses of SN-38 for 48 h, the knockdown effects on ATG5 were confirmed by Western blot analysis (upper panel). Cell viability was measured using CCK8 assay. Columns, means of three determinations; bars, SD.

    Free Radic Biol Med, 2017, 104:280-297. SN-38 purchased from Selleck.

    HCT116 cells were pretreated with tested compounds for 1 hour and then cotreated with 1 μM SN-38 for 2 hours. Cell lysates were then subjected to Western blot analysis. Data shown are representative of three independent experiments. Con, concentration.

    J Pharmacol Exp Ther 2014 348(3), 432-41. SN-38 purchased from Selleck.

Purity & Quality Control

Choose Selective Topoisomerase Inhibitors

Biological Activity

Description SN-38 is an active metabolite of CPT-11, inhibits DNA topoisomerase I, DNA synthesis and causes frequent DNA single-strand breaks.
Targets
Topo I [1]
(Cell-free assay)
In vitro

SN-38, a biological active metabolite of irinotecan hydrochloride (CPT-11). SN-38 causes the strongest inhibition of DNA topoisomerase I, followed by CPT and then CPT-11. CPT-11 dose dependently shifts the position of relaxed DNA in the direction of nicked DNA, but SN-38 and CPT shows no effect on the position of relaxed DNA. SN-38 dose-dependently and time-dependently inhibit DNA synthesis. Respective IC50 values of SN-38, in DNA synthesis is 0.077 μM. The inhibitory effect of SN-38 on RNA synthesis is less than that on DNA synthesis and it does not inhibit protein synthesis. SN-38 caused frequent DNA single-strand breaks in P388 cells. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human PC6 cells MkXiVJJwdGmoZYLheIlwdiCjc4PhfS=> NXHhTYU6PiCmYYnz NYizNI5USW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDQR|Yh[2WubIOgZ4FzenmrbnegdHJEKGGodHXyJFYh\GG7czygTWM2OD1yLkSzJI5O NUnwbVQ2OTl{NUS4OFM>
human HCT116 cells MX;Qdo9tcW[ncnH0bY9vKGG|c3H5 M4TpZVMh\GG7cx?= NH7BVY1CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjDWFEyPiClZXzsd{Bi\nSncjCzJIRigXNuIFnDOVA:OC53NTDuUS=> NHrGeI8yQTJ3NEi0Ny=>
human PC3 cells M3TVPWN6fG:2b4jpZ:Kh[XO|YYm= MnvEO|IhcA>? NHXzd2FEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBRSzNiY3XscJMh\XiycnXzd4lv\yCjbIDoZVVj\XSjMzDpcpRm\3KrbjDhd5Nme3OnZDDhd{Bk\WyuIIP1dpZqfmGuIHHmeIVzKDd{IHjyd{BjgSCVUlKgZZN{[XluIFnDOVA:Oi54IH7N NWLlT3ZtOjJ7NUmyOFY>
human A549 cells NVPOWotoS3m2b4TvfIlkyqCjc4PhfS=> NWjQV3NtOyCmYYnz NHXRWlBEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCPTR7IHPlcIx{KGG|c3Xzd4VlKGG|IHfyc5d1cCCrbnjpZol1cW:wIHHmeIVzKDNiZHH5d{BjgSCVUlKgZZN{[XluIFnDOVA:Oi55MjDuUS=> MXyyOFUzQTh5MB?=
human QG56 cells MY\Qdo9tcW[ncnH0bY9vKGG|c3H5 M17aVFMh\GG7cx?= MljZRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCTR{W2JINmdGy|IHHmeIVzKDNiZHH5d{whUUN3ME2yMlghdk1? MkTpNVkzPTR6NEO=
human NCI-H460 cells NXfSOIU1WHKxbHnm[ZJifGmxbjDhd5NigQ>? NV;VWnk3OyCmYYnz NVrW[pFwSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDOR2kuUDR4MDDj[YxteyCjZoTldkA{KGSjeYOsJGlEPTB;Mz6zJI5O M2X4flE6OjV2OESz
human DU145 cells M4PlR3Bzd2yrZnXyZZRqd25iYYPzZZk> M2XaXlk3KGh? NIGzNY1CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFTVNVQ2KGOnbHzzJIFnfGW{IEm2JIhzeyxiSVO1NF01KG6P M1jWfFE5Ojd4MUSx
human breast cancer cell line (SK-BR-3) M1fmR2N6fG:2b4jpZ:Kh[XO|YYm= NFvmUXBKdiCYaYTyc{BkgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBjemWjc4SgZ4Fv[2W{IHPlcIwhdGmwZTCoV2suSlJvMzmsJGlEPTB;NDDuUS=> M{f2ZlEyOzN2NU[5
human KB3-1 cells NH3nVWhEgXSxdH;4bYPDqGG|c3H5 NHvJU3U1KGSjeYO= NXHoeZMxS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hU0J|LUGgZ4VtdHNiYX\0[ZIhPCCmYYnzJIJ6KE2WVDDt[ZRpd2RuIFnDOVA:PCCwTR?= MkG1NVk{ODN|ME[=
human HCT116 cells MWjDfZRwfG:6aXRCpIF{e2G7 M4L6cFczKGh? NEDGVWZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJS1RzMU[gZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KHO3bH\vdohw\GGvaX7lJGIh[XO|YYmsJGlEPTB;ND6yPEBvVQ>? MnjpNlU5OzV|NUm=
MDA-MB-435 S human breast cancer cells M2fsZmZ2dmO2aX;uJIF{e2G7 MV;Jcohq[mm2aX;uJIFo[Wmwc4SgUWRCNU2ELUSzOUBUKGi3bXHuJIJz\WG|dDDjZY5k\XJiY3XscJMhcW5idHjlJIFje2WwY3Wgc4Yh[WykdX3pckwhUUN3ME21JI5O MUCxNVA2OjhyMh?=
human HT-29 cells MnHrR5l1d3SxeHnjxsBie3OjeR?= NETzWJBEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJXC1{OTDj[YxteyxiSVO1NF02Njlibl2= NX\GdmFmOjF2N{C4OlQ>
human lung cancer cell line (H128) NVHNNmlzS3m2b4TvfIlkyqCjc4PhfS=> NF;wfXdKdiCYaYTyc{BkgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBtfW6pIHPhcoNmeiClZXzsJIxqdmViKFixNlgqNCCLQ{WwQVYhdk1? NEnPTVcyOTN|NEW2PS=>
human MIA PaCa cells MkO4VJJwdGmoZYLheIlwdiCjc4PhfS=> NV3XelVSSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNTWEhWGGFYTDj[YxteyCjZoTldkA6PiCqcoOsJGlEPTB;NjDuUS=> NEGyRVgyQDJ5NkG0NS=>
human HCT116 colon cancer cell line NIXmZ4VHfW6ldHnvckBie3OjeR?= M{nWWGlvcGmkaYTvdpkh[2:wY3XueJJifGmxbjDh[4FqdnO2IHj1cYFvKEiFVEGxOkBkd2yxbjDjZY5k\XJiY3XscEBtcW6nLDDJR|UxRTdibl2= NWfUSpZFOTV6MEi0OVY>
human stomach cancer cell line (MKN45) NYTHcmgxS3m2b4TvfIlkyqCjc4PhfS=> NEX4cJpKdiCYaYTyc{BkgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckB{fG:vYXPoJINidmOncjDj[YxtKGyrbnWgLG1MVjR3KTygTWM2OD16IH7N MVKxNVM{PDV4OR?=
human A2780 cells NG\WeJdEgXSxdH;4bYPDqGG|c3H5 NF\yUFg4OiCq M4KyemN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGEzPzhyIHPlcIx{KG:4ZYLlfJBz\XO|aX7nJIFteGijNXLleIE{KGmwdHXndolvKGG|c3Xzd4VlKGG|IHPlcIwhe3W{dnn2ZYwh[W[2ZYKgO|IhcHK|IHL5JHNTSiCjc4PhfUwhUUN3ME25JI5O MWCyNlk2QTJ2Nh?=
human ovarian cancer cell line (SK-OV-3) MknRR5l1d3SxeHnjxsBie3OjeR?= MlHmTY4hXmm2cn:gZ5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gc5ZiemmjbjDjZY5k\XJiY3XscEBtcW6nIDjTT{1QXi1|KTygTWM2OD1zMTDuUS=> MWKxNVM{PDV4OR?=
human DU145 prostate cell line M4PQUXBzd2yrZnXyZZRqd25iYYPzZZk> M2LzRmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iRGWxOFUheHKxc4TheIUh[2WubDDsbY5mNCCLQ{WwQVE{KG6P MXyxNFQ6QDJzNh?=
human colon cancer cell line (WiDr) NGOzb3VEgXSxdH;4bYPDqGG|c3H5 M2TZVmlvKF[rdILvJIN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJINwdG:wIHPhcoNmeiClZXzsJIxqdmViKGfpSJIqNCCLQ{WwQVE1KG6P M2\MZVEyOzN2NU[5
human lung cancer cell line (A549) NHW2PJNEgXSxdH;4bYPDqGG|c3H5 NVXZVXlHUW5iVnn0do8h[3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hdHWwZzDjZY5k\XJiY3XscEBtcW6nIDjBOVQ6MQ>? MkPsNVE{OzR3Nkm=
human tumor HL60 cell-line MY\GeY5kfGmxbjDhd5NigQ>? NVrRNVBpOyCmYYnz NIroO|FKdi24aYTyc{BqdmirYnn0c5J6KGOxbnPlcpRz[XSrb36g[o9zKGi3bXHuJJR2dW:{IFjMOlAh[2WubD3sbY5mKHejczDk[ZRmem2rbnXkJJV{cW6pIGPSRkBie3OjeTDh[pRmeiB|IHThfZMhd2ZiaX7jeYJifGmxbjygTWM2OD1zOTDuUS=> NV7MbYM3OTV7MUO5PVY>
PC-3 carcinoma cell line MYXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M2D3VmNwdmOnboTyZZRqd25icnXxeYlz\WRidH:gbY5pcWKrdDDndo94fGhib3[gbJVu[W5icILvd5RifGViUFOtN{Bk[XKlaX7vcYEh[2WubDDsbY5mNCCLQ{WwQVM2NjZibl2= NIPG[lMyPTR3NEKzNC=>
human KBV1 cells M{KzVGN6fG:2b4jpZ:Kh[XO|YYm= NFvWfVA1KGSjeYO= NVr6flhrS3m2b4TvfIlkcXS7IHHnZYlve3RiTVTSNUBwfmW{ZYjwdoV{e2mwZzDoeY1idiCNQm[xJINmdGy|IHHmeIVzKDRiZHH5d{BjgSCPVGSgcYV1cG:mLDDJR|UxRTR4IH7N NV;iPY44OTl|MEOzNFY>
human A549 cells NH3jSGJEgXSxdH;4bYPDqGG|c3H5 M1KwemN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGE2PDliY3XscJMtKEmFNUC9OFchdk1? M1fsO|IyPDdyOE[0
NSCLC-H460 NFmzbYZEgXSxdH;4bYPDqGG|c3H5 MVzDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDuc44ue22jbHytZ4VtdCCudX7nJINiemOrbn;tZUBk\WyuIHzpcoUhUDR4MDCoUnNEVENvSES2NEktKEmFNUC9PFAhdk1? MnriNVE2PjN7MkW=
MDA-MB-435 S human breast cancer cells M1jJemZ2dmO2aX;uJIF{e2G7 M{nFN2lvcGmkaYTpc44h[WejaX7zeEBOTEFvTVKtOFM2KFNiaIXtZY4h[nKnYYP0JINidmOncjDj[YxteyCrbjD0bIUheHKnc3XuZ4Uhd2ZiM{CgcYcwdUxiSGPBMEBKSzVyPUWwJI5O MlvNNVExPTJ6MEK=
human H460 cell MWLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MX7Jcohq[mm2aX;uJI9nKGi3bXHuJGg1PjBiY3XscEBoem:5dHisJGlEPTB;OECgcm0> NFnaU24yPjlzM{ewOi=>
human MDA-MB-231 cells M3v2PGN6fG:2b4jpZ:Kh[XO|YYm= M1zT[mN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1FSS2PQj2yN|Eh[2WubIOsJGlEPTB;MUe2JI5O NHTOO|AzPDV{OUi3NC=>
human KBH5.0 cells NXOyPWpWS3m2b4TvfIlkyqCjc4PhfS=> Mn;kOEBl[Xm| NXLGZ3YyS3m2b4TvfIlkcXS7IHHnZYlve3RiQlPSVEBwfmW{ZYjwdoV{e2mwZzDoeY1idiCNQli1MlAh[2WubIOgZYZ1\XJiNDDkZZl{KGK7IF3UWEBu\XSqb3SsJGlEPTB;MD6zJO69VQ>? MVexPVMxOzNyNh?=
human MCF-7 breast cell line M1XkSnBzd2yrZnXyZZRqd25iYYPzZZk> NWrRZot2SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNR2YuPyCkcnXhd5Qh[2WubDDsbY5mNCCLQ{WwQVAvOzdizszN MmnINVA1QTh{MU[=
human SKOV-3 ovarian cell line NWPvV3ZwWHKxbHnm[ZJifGmxbjDhd5NigQ>? M2jiUWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iU1vPWk0{KG:4YYLpZY4h[2WubDDsbY5mNCCLQ{WwQVAvPzJizszN NH[yU48yODR7OEKxOi=>
human Bel-7402 liver cancer cell line NVHJVZc4TnWwY4Tpc44h[XO|YYm= NHrrNGlKdmirYnn0c5J6KGOxbnPlcpRz[XSrb36gZYdicW6|dDDoeY1idiCEZXytO|QxOiCuaY\ldkBk[W6lZYKgZ4VtdCCuaX7lMEBKSzVyPUOuNVkh|ryP M3zndlE2QDB6NEW2
HEK293 cells NGH4WWxEgXSxdH;4bYPDqGG|c3H5 Mlr3O|IhcA>? M3\lU2lvfHKrboPpZ{BkgXSxdH;4bYNqfHliYXfhbY5{fCCKRVuyPVMh[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIHPlcIwhfmmjYnnsbZR6KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZk> NHK0[YszPTJ5MkC1OS=>

... Click to View More Cell Line Experimental Data

In vivo After oral dosing, peak SN-38 concentrations occurrs within 1 h, and the The percent unbound SN-38 lactone in murine plasma at 1000 ng/mL is 3.4 +/- 0.67%, whereas at 100 ng/mL the percent unbound is 1.18 +/- 0.14%. SN-38 lactone AUCs in micebearing human neuroblastoma xenografts are greater than in nontumor-bearing animals. [2]

Protocol

Kinase Assay:[1]
+ Expand

Topoisomerase I Assay:

One unit (the minimum amount for full relaxation of 0.5 μg SV40 DNA under the conditions of this study) of topoisomerase I, 0.5 μL of the test compounds, and 0.5μg SV40 DNA are added sequentially to the reaction buffer, which is composed of 25 mM Tris-HCl (pH 7.5), 50 mM KC1, 5 mM MgCl2, 0.25 mM EDTA disodium salt, 0.25 mM dithiothreitol, 15μg /mL bovine serum albumin, and 5% glycerol. Then, the reaction mixture (50 μL) is incubated for 10 min at 37 °C, and the reaction is terminated by treatment with 7.5 μL of a solution consisting of 1% sodium dodecyl sulfate, 20 mM EDTA disodium salt, and 0.5 mg/mL proteinase K for an additional 30 min at 37°C. The samples are mixed with 5 μL of the loading buffer containing 10 mM Na2HPO4, 31.3% sucrose, and 0.3% bromophenol blue. Relaxed (form Ir) DNA is separated from supercoiled (form I) and nicked (form II) DNA by electrophoresis on 0.8% agarose gel at 50 mA and 20 V for 17 h in the presence of 2 μg/mL chloroquine, 10 mM EDTA, 30 mM NaH2PO4, and 36 Mm Tris-HCl (pH 7.8). After electrophoresis, the gel is stained with 0.05% ethidium bromide and photographed with UV light (302 nm). The amount of DNA is quantified using a densitometer.
Cell Research:[3]
+ Expand
  • Cell lines: A-172, U-87, and LA-567
  • Concentrations: 0 -1000 nM
  • Incubation Time: 48 h
  • Method: MTT assay
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 21 mg/mL (53.51 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 392.4
Formula

C22H20N2O5

CAS No. 86639-52-3
Storage powder
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT00311610 Completed Colorectal Cancer Alliance for Clinical Trials in Oncology|National Cancer Institute (NCI) January 2006 Phase 2
NCT00046540 Completed Neoplasms INSYS Therapeutics Inc October 2002 Phase 1
NCT00104754 Withdrawn Lung Cancer Alliance for Clinical Trials in Oncology|National Cancer Institute (NCI) null Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Topoisomerase Signaling Pathway Map

Related Topoisomerase Products

Tags: buy SN-38 | SN-38 supplier | purchase SN-38 | SN-38 cost | SN-38 manufacturer | order SN-38 | SN-38 distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID