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Lycorine hydrochloride ADC Cytotoxin inhibitor

Name: Lycorine hydrochloride
Brand: Selleck Chemicals
SKU: S3800
Availability: InStock
Rating: 5 (1 reviews)

Cat.No.S3800

Lycorine (Galanthidine, Amarylline, Narcissine, Licorine), a natural alkaloid extracted from the Amaryllidaceae plant family, has been reported to exhibit a wide range of physiological effects, including the potential effect against cancer. It is an HCV inhibitor with strong activity.

Chemical Structure

Molecular Weight: 323.77

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Quality Control

Batch: Purity: 99.81%

99.81

Related Targets	HDAC Caspase Proteasome Secretase MMP HCV Protease Cysteine Protease DPP Tyrosinase HIV Protease
Other ADC Cytotoxin Inhibitors	SN-38 Triptolide (+)-Bicuculline Rutin Artemisinin Pinocembrin BHQ Harmine hydrochloride Psoralen Lappaconite HBr

Solubility

In vitro
Batch:

DMSO : 65 mg/mL (200.75 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	3.250mg/ml (10.04mM)	Taking the 1 mL working solution as an example, add 50 μL of 65 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.500mg/ml (1.54mM)	Taking the 1 mL working solution as an example, add 50 μL of 10 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	3.250mg/ml (10.04mM)	Taking the 1 mL working solution as an example, add 50 μL of 65 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.800mg/ml (2.47mM)	Taking the 1 mL working solution as an example, add 50 μL of 16 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	323.77	Formula	C₁₆H₁₇NO₄.HCl	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	2188-68-3	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	Galanthidine hydrochloride, Amarylline hydrochloride, Narcissine hydrochloride, Licorine hydrochloride			Smiles	C1CN2CC3=CC4=C(C=C3C5C2C1=CC(C5O)O)OCO4.Cl

Mechanism of Action

In vitro	Lycorine not only possesses strong pharmacological effects on many diseases, including anti-leukemia, anti-tumor, anti-angiogenesis, anti-virus, anti-bacteria, anti-inflammation, and anti-malaria, but also exerts many other biological functions, such as inhibition of acetylcholinesterase and topoisomerase, suppression of ascorbic acid biosynthesis, and control of circadian period length. Lycorine inhibits cell proliferation and induces cell apoptosis in acute myeloid leukemia (AML) cell line HL-60, monocyte leukemia cell line U937, and T-cell leukemia cell line Jurkat. Lycorine also blocks multiple myeloma KM3 cell cycle, induces K562 cell-cycle arrest at the G0/G1 phase and suppresses tumorigenesis and the growth of various tumor cells, including melanoma C8161, ovarian cancer Hey1B, lung cancer A549, glioblatatoma U373, and esophageal cancer OE21. Lycorine can inhibit protein synthesis in eukaryotic cells, and acetylcholinesterase activity.
In vivo	Lycorine exhibits its numerous pharmacological effects on various diseases with very low toxicity and mild side effects. In vivo studies, lycorine effectively inhibits tumor growth in several mouse xenograft models. It effectively inhibits tumor neovascularization. Lycorine has trivial toxicity to normal cells and normal bodies. The animal toxicological experiments show that the 50% lethal death rate (LD50) of lycorine in mice is 112.2±0.024 mg/kg via intraperitoneal injection. The LD50 of lycorine in mice is 344 mg/kg via gastric lavage injection, suggesting that lycorine is very low toxicity in gastrointestinal administration. In vivo studies show that lycorine has linear plasma kinetics with a mean elimination half-life of 0.67 and 0.3 h after single s.c. and i.v. administration, respectively. The mean oral bioavailability is calculated to be about 40%. Biochemical and hematological parameters of safety studies indicate no pathological signs.
References	[1] https://link.springer.com/article/10.1007/s11426-013-4967-9 [2] https://pubmed.ncbi.nlm.nih.gov/17336028/ [3] https://pubmed.ncbi.nlm.nih.gov/23176676/

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