CI994 (Tacedinaline)

Catalog No.S2818 2 Product Use Citation

CI994 (Tacedinaline) is an anti-cancer drug which inhibits HDAC1 with IC50 of 0.57 μM in a cell-free assay and causes G1 cell cycle arrest. Phase 3.

Price Stock Quantity  
USD 90 In stock
USD 70 In stock
USD 270 In stock
Bulk Inquiry

Massive Discount Available

Free Overnight Delivery on all orders over $ 500.

CI994 (Tacedinaline) Chemical Structure

CI994 (Tacedinaline) Chemical Structure
Molecular Weight: 269.3

Validation & Quality Control

Quality Control & MSDS

Related Compound Libraries

CI994 (Tacedinaline) is available in the following compound libraries:

Product Information

  • Compare HDAC Inhibitors
    Compare HDAC Products
  • Research Area
  • Combination Therapy
    Combination Therapy

Product Description

Biological Activity

Description CI994 (Tacedinaline) is an anti-cancer drug which inhibits HDAC1 with IC50 of 0.57 μM in a cell-free assay and causes G1 cell cycle arrest. Phase 3.
Targets HDAC1 [1]
(Cell-free assay)
IC50 0.57 μM
In vitro CI-994 (< 160 mM) shows cytostatic effect with concomitant increase at G0/G1 phase, a reduction at S phase level and increased apoptosis in A-549 and LX-1 cells. [2] CI-994 inhibits growth of LNCaP cell with IC50 of 7.4 μM. [3] CI-994 exerts activity against several tumor cell lines with greater cytotoxicity against the solid tumors relative to both the leukemia and normal fibroblast cell lines. [4] CI-994 inhibits growth of rat leukemia BCLO cells with IC50 of 2.5 μM. [5]
In vivo CI-994 exerts demonstrated antitumor activity against several tumor models, including the chemo-resistant mouse pancreatic ductal carcinoma as well as the human prostate tumor model LNCaP. [4]

Protocol(Only for Reference)

Cell Assay: [3]

Cell lines LNCaP cell lines
Concentrations 2.5 μM
Incubation Time 2-4 days
Method LNCaP cell lines are maintained in RPMI 1640 medium containing 10% fetal bovine serum, 1% penicillin and streptomycin, as the complete culture medium. Cells (2×104) are seeded in 24-well plates and incubated in a 5% CO2 incubator at 37 °C for 1 day. Cultures are treated with CI-994, alone and in combination on day 2 and 4. Cells are washed on day 2 and 4 and media are changed. Mitochondrial metabolism is measured as a marker for cell growth by adding 100 μL/well MTT (5 mg/mL in medium) with 2 hours incubation at 37 °C on Day 6. Crystals formed are dissolved in 500 μL of DMSO. The absorbance is determined using a microplate reader at 560 nm. The absorbance data are converted into cell proliferation percentage. Each assay is performed in triplicate.

Animal Study: [4]

Animal Models human prostate tumor model LNCaP
Formulation 5% ethanol, 1% P.O.E. and 94% dH20
Dosages 535 mg/kg
Administration Administered via oral gavage

Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)

SpeciesMouseRatRabbitGuinea pigHamsterDog
Weight (kg)
Body Surface Area (m2)0.0070.0250.
Km factor36128520
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Rat dose (mg/kg) = mouse dose (22.4 mg/kg) ×  mouse Km(3)  = 11.2 mg/kg
rat Km(6)


[1] Methot JL, et al. Bioorg Med Chem Lett, 2008, 18(3), 973-978.

[2] Loprevite M, et al. A Oncol Res, 2005, 15(1), 39-48.

view more

Clinical Trial Information( data from, updated on 2015-11-14)

NCT Number Recruitment Conditions Sponsor
Start Date Phases
NCT00005093 Completed Lung Cancer Pfizer December 1999 Phase 3
NCT00004861 Completed Pancreatic Cancer Pfizer October 1999 Phase 2
NCT00005624 Completed Multiple Myeloma H. Lee Moffitt Cancer Center and Research Institute|Natio  ...more H. Lee Moffitt Cancer Center and Research Institute|National Cancer Institute (NCI)|Parke-Davis August 1997 Phase 2

Chemical Information

Download CI994 (Tacedinaline) SDF
Molecular Weight (MW) 269.3


CAS No. 112522-64-2
Storage 3 years -20℃powder
6 months-80℃in solvent
Synonyms PD-123654, GOE-5549, Acetyldinaline
Solubility (25°C) * In vitro DMSO 54 mg/mL (200.51 mM)
Water <1 mg/mL (<1 mM)
Ethanol <1 mg/mL (<1 mM)
In vivo 30% PEG400/0.5% Tween80/5% propylene glycol 30 mg/mL
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
Chemical Name Benzamide, 4-(acetylamino)-N-(2-aminophenyl)-

Product Use Citation (2)

Tech Support & FAQs

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

* Indicates a Required Field

Related HDAC Products

  • LMK-235

    LMK-235 is a selective inhibitor of HDAC4 and HDAC5 with IC50 of 11.9 nM and 4.2 nM, respectively.

  • CAY10603

    CAY10603 is a potent and selective HDAC6 inhibitor with IC50 of 2 pM, >200-fold selectivity over other HDACs.

  • 4SC-202

    4SC-202 is a selective class I HDAC inhibitor with IC50 of 1.20 μM, 1.12 μM, and 0.57 μM for HDAC1, HDAC2, and HDAC3, respectively. Also displays inhibitory activity against Lysine specific demethylase 1 (LSD1). Phase 1.

  • Panobinostat (LBH589)

    Panobinostat (LBH589) is a novel broad-spectrum HDAC inhibitor with IC50 of 5 nM in a cell-free assay. Phase 3.

  • Vorinostat (SAHA, MK0683)

    Vorinostat (suberoylanilide hydroxamic acid, SAHA) is an HDAC inhibitor with IC50 of ~10 nM in a cell-free assay.

  • Entinostat (MS-275)

    Entinostat (MS-275) strongly inhibits HDAC1 and HDAC3 with IC50 of 0.51 μM and 1.7 μM in cell-free assays, compared with HDACs 4, 6, 8, and 10. Phase 3.

  • Trichostatin A (TSA)

    Trichostatin A (TSA) is an HDAC inhibitor with IC50 of ~1.8 nM in cell-free assays.

  • Romidepsin (FK228, Depsipeptide)

    Romidepsin (FK228, depsipeptide) is a potent HDAC1 and HDAC2 inhibitor with IC50 of 36 nM and 47 nM in cell-free assays, respectively.

    Features:More effective than other classical HDAC inhibitors such as TSA, TPX, and butyrate.

  • RGFP966

    RGFP966 is an HDAC3 inhibitor with IC50 of 0.08 μM in cell-free assay, exhibits > 200-fold selectivity over other HDAC.

  • Tubastatin A

    Tubastatin A is a potent and selective HDAC6 inhibitor with IC50 of 15 nM in a cell-free assay. It is selective against all the other isozymes (1000-fold) except HDAC8 (57-fold).

Recently Viewed Items

Tags: buy CI994 (Tacedinaline) | CI994 (Tacedinaline) supplier | purchase CI994 (Tacedinaline) | CI994 (Tacedinaline) cost | CI994 (Tacedinaline) manufacturer | order CI994 (Tacedinaline) | CI994 (Tacedinaline) distributor
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Contact Us