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Choline Chloride AChR agonist

Name: Choline Chloride
Brand: Selleck Chemicals
SKU: S4171
Availability: InStock
Rating: 5 (1 reviews)

Cat.No.S4171

Choline chloride(Hepacholine) is a quaternary ammonium salt used as an additive for animal feed. It is important as a precursor of acetylcholine, as a methyl donor in various metabolic processes, and in lipid metabolism.

Chemical Structure

Molecular Weight: 139.62

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Quality Control

Batch: S417101 DMSO]28 mg/mL]false]Water]28 mg/mL]false]Ethanol]28 mg/mL]false Purity: 100.0%

Cited in Nature Medicine for its top-tier quality
COA
NMR
HPLC
SDS
Datasheet

100.0

Related Targets	Adrenergic Receptor 5-HT Receptor COX Calcium Channel Histamine Receptor Dopamine Receptor GABA Receptor TRP Channel Cholinesterase (ChE) GluR
Other AChR Inhibitors	PNU-120596 Benzethonium Chloride Arecoline HBr Lycorine Otilonium Bromide Hyoscyamine (-)-Huperzine A (HupA) Nitenpyram Cytisine Palmatine

Solubility

In vitro
Batch:

DMSO : 28 mg/mL (200.54 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : 28 mg/mL

Ethanol : 28 mg/mL

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	139.62	Formula	C₅H₁₄NO.Cl	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	67-48-1	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	Hepacholine			Smiles	C[N+](C)(C)CCO.[Cl-]

Mechanism of Action

In vitro

Choline is a major part of the polar head group of phosphatidylcholine and a major part of another membrane phospholipid, sphingomyelin, which are very important for maintenance of cell structure and function. Choline deficiency in cell culture causes apoptosis or programmed cell death. Betaine or trimethylglycine is derived from choline via an oxidation reaction. Acetylcholine is one of the major neurotransmitters and requires choline for its synthesis. Adequate acetylcholine levels in the brain are believed to be protective against certain types of dementia, including Alzheimer's disease. Choline is also needed for gallbladder regulation, liver function and lecithin (a key lipid) formation. Choline also aids in fat and cholesterol metabolism and prevents excessive fat build up in the liver. Choline has been used to mitigate the effects of Parkinsonism and tardive dyskinesia. Choline deficiencies may result in excessive build-up of fat in the liver, high blood pressure, gastric ulcers, kidney and liver dysfunction and stunted growth.

In vivo

Choline chloride is of very low acute oral toxicity. The oral LD50 in rats is determined to be between 3150 and 5000 mg/kg bw. Clinical symptoms after application are restlessness, increased frequency of respiration, hypoactivity, convulsions, ruffled coat, staggered gait and dyspnoea. Some animals have diarrhoea and at necropsy in the high dose groups in one study 3 out of 10 rats have inflamed lungs.

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2024-05-22)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT06379971	Not yet recruiting	Child Development\|Cannabis Use	University of Colorado Denver	May 2024	Phase 1
NCT06120400	Not yet recruiting	Cardiovascular Diseases	Penn State University\|American Egg Board	May 2024	Phase 3
NCT06405360	Not yet recruiting	Infant Malnutrition\|Fetal Growth Retardation	University of Florida\|World Vision\|University of Rwanda	May 2 2024	Not Applicable
NCT06208085	Recruiting	Breastfeeding Exclusive	University of North Carolina Chapel Hill\|Avocado Nutrition Center	February 8 2024	Not Applicable

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