Home DNA Damage/DNA Repair HDAC inhibitor BRD3308 For research use only.

BRD3308

BRD3308 is a potant and highly selective inhibitor of HDAC3 with IC50 of 54 nM, 1.26 μM and 1.34 μM for HDAC3, HDAC1 and HDAC2, respectively. BRD3308 activates HIV-1 transcription. BRD3308 suppresses pancreatic β-cell apoptosis induced by inflammatory cytokines (glucolipotoxic stress) and increases functional insulin release.

BRD3308 Chemical Structure

BRD3308 Chemical Structure

CAS: 1550053-02-5

Purity & Quality Control

Batch: S896201 DMSO] 57 mg/mL] false] Water] Insoluble] false] Ethanol] Insoluble] false Purity: 98.19%
98.19

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Biological Activity

Description BRD3308 is a potant and highly selective inhibitor of HDAC3 with IC50 of 54 nM, 1.26 μM and 1.34 μM for HDAC3, HDAC1 and HDAC2, respectively. BRD3308 activates HIV-1 transcription. BRD3308 suppresses pancreatic β-cell apoptosis induced by inflammatory cytokines (glucolipotoxic stress) and increases functional insulin release.
Targets
HDAC3 [1]
(Cell-free assay)		 HDAC1 [1]
(Cell-free assay)		 HDAC2 [1]
(Cell-free assay)
54 nM 1.26 μM 1.34 μM
In vitro
In vitro

BRD3308 is a derivative of the ortho-aminoanilide HDAC inhibitor CI-994 and is developed to be highly selective for inhibition of HDAC3 with an IC50 value that is 23-fold lower for HDAC3 than for HDAC1 or 22. HDAC3 selective inhibition induces expression of HIV and exposure to BRD3308 allows the recovery of latent HIV-1 from patient cells.[1]
Cell Research Cell lines 2D10 cells, J89 cells, THP89 cells, J-Lat 6.3 cells
Concentrations 5 μM, 10 μM, 15 μM, 30 μM
Incubation Time 6 h, 12 h, 18 h, 24 h
Method

BRD3308 (15 μM) is added to aliquots of resting CD4+ T cells which are then plated in limiting dilution replicates of 2.5 or 1 million, 0.5 million, and 0.1 million cells and incubated at 37℃ under 5% CO2. BRD3308 is removed from the cultures after 24 hours.
In Vivo
In vivo

Selective inhibition of HDAC3 by BRD3308 prevents diabetes onset in female NOD mice. HDAC3 treatment in vivo prevents pancreatic islet infiltration and protects β-cells from apoptosis. β-cell proliferation is increased in animals treated with BRD3308. HDAC3 treatment in vivo prevents white adipose tissue infiltration in NOD mice.[2]
Animal Research Animal Models Female NOD/ShiLTJ (NOD) mice
Dosages 0.1 mg/kg, 1 mg/kg, 10 mg/kg
Administration IP

Chemical Information & Solubility

Molecular Weight 287.29 Formula

C15H14FN3O2
CAS No. 1550053-02-5 SDF --
Smiles CC(=O)NC1=CC=C(C=C1)C(=O)NC2=C(C=C(C=C2)F)N
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 57 mg/mL ( (198.4 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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