Name: Sodium butyrate
Brand: Selleck Chemicals
SKU: S1999
Rating: 5 (25 reviews)

Sodium butyrate (NaB, Butanoic acid sodium salt), sodium salt of butyric acid, is a histone deacetylase inhibitor and competitively binds to the zinc sites of class I and II histone deacetylases (HDACs). Sodium butyrate (NaB) inhibits cell cycle progression, promotes differentiation, and induces apoptosis and autophagy in several types of cancer cells.

Sodium butyrate Chemical Structure

CAS: 156-54-7

Selleck's Sodium butyrate has been cited by 25 Publications

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Purity & Quality Control

Batch: Purity: 100.00%

100.00

Sodium butyrate Related Products

Related Targets	HDAC1 HDAC2 HDAC3 HDAC4 HDAC5 HDAC6 HDAC7 HDAC8 HDAC9 HDAC10 HDAC11 HD1 HD2	Click to Expand
Related Compound Libraries	Kinase Inhibitor Library FDA-approved Drug Library Natural Product Library Bioactive Compound Library-I Bioactive Compound Library-Ⅱ	Click to Expand

Signaling Pathway

HDAC Signaling Pathway

Choose Selective HDAC Inhibitors

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
human K562 cells	Proliferation assay	1 mM		Antiproliferative activity against human K562 cells at 1 mM in presence of guanosine	23937979
human H1299 cells	Function assay		7 days	Inhibition of human USP1/UAF1 in human H1299 cells assessed as reduction in colony formation after 7 days, EC50=3 μM	25229643
human JEG3 cells	Function assay			Inhibition of aromatase in human JEG3 cells by scintillation spectrometry, IC50=0.0015 μM	20148564
Click to View More Cell Line Experimental Data

Biological Activity

Description

Sodium butyrate (NaB, Butanoic acid sodium salt), sodium salt of butyric acid, is a histone deacetylase inhibitor and competitively binds to the zinc sites of class I and II histone deacetylases (HDACs). Sodium butyrate (NaB) inhibits cell cycle progression, promotes differentiation, and induces apoptosis and autophagy in several types of cancer cells.

Targets

Histone deacetylase ^[6]

In vitro
In vitro	Butyrate mediates growth inhibition of colonic cancer cells and thereby to elucidate the molecular link between a high-fiber diet and the arrest of colon carcinogenesis. Butyrate induces p21 mRNA expression in an immediate-early fashion, through transactivation of a promoter cis-element(s) located within 1.4 kb of the transcriptional start site, independent of p53 binding. ^[1] Sodium butyrate, at physiological concentrations, induces apoptosis in 2 adenoma cell lines, RG/C2 and AA/Cl, and in the carcinoma cell line PC/JW/FI. ^[2] Butyrate exerts potent effects on a variety of colonic mucosal functions such as inhibition of inflammation and carcinogenesis, reinforcing various components of the colonic defence barrier and decreasing oxidative stress. ^[3] Butyrate inhibits most HDAC except class III HDAC and class II HDAC6 and -10. Butyrate also is the most effective fatty acid in stimulating or repressing the expression of specific genes. ^[4]
Experimental Result Images	Methods	Biomarkers	Images	PMID
	Western blot	Cyclin D3 / CDK4 / CDK6 / CDK2 p15INK4b / p21Cip1 Phospho-H3 Ser10 / Acetyl-H3Lys9 / Acetyl-H3Lys14 / Dimethyl-H3		24280698
	Immunofluorescence	Acetyl-H3Lys9		24280698

In Vivo
In vivo	Sodium butyrate significantly extends survival in a dose-dependent manner, improves body weight and motor performance, and delays the neuropathological sequelae in the R6/2 transgenic mouse model of Huntington's disease (HD). Sodium butyrate also increases histone and specificity protein-1 acetylation and protects against 3-nitropropionic acid neurotoxicity. ^[5]

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-01-11)
NCT00800930	Completed	Shigellosis	International Centre for Diarrhoeal Disease Research Bangladesh\|Swedish International Development Cooperation Agency (SIDA)\|Karolinska Institutet	January 2005	Phase 2

References

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Chemical Information & Solubility

Molecular Weight	110.09	Formula	C₄H₇NaO₂
CAS No.	156-54-7	SDF	Download Sodium butyrate SDF
Smiles	CCCC(=O)[O-].[Na+]
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vitro
Batch:

Water : 22 mg/mL

Ethanol : 8 mg/mL

DMSO : Insoluble ( Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

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In vivo
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