Indomethacin

Catalog No.S1723

Indomethacin  Chemical Structure

Molecular Weight(MW): 357.79

Indomethacin is a nonselective COX1 and COX2 inhibitor with IC50 of 0.1 μg/mL and 5 μg/mL, respectively, used to reduce fever, pain, stiffness, and swelling.

Size Price Stock Quantity  
In DMSO USD 130 In stock
USD 97 In stock
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1 Customer Review

  • Indicated PN and MES spheres were shown expressing GFP under the control of a 7 × TCF/LEF optimal promoter cassette (7 × TOP) and constitutively expressed nuclear mCherry. PN 84 and MES 1123 were treated with Wnt3a (200 ng/ml), indomethacin (Indo; 20 μM), or a control (vehicle). Scale bar, 100 μm. Bar graph, quantification of fluorescent signal of GFP (TOP-GFP) versus mCherry (PGK-H2BmCherry). The percentage of GFP- and mCherry-positive spheres was determined by FACS.

    Nat Commun, 2016, 7:12885. Indomethacin purchased from Selleck.

Purity & Quality Control

Choose Selective COX Inhibitors

Biological Activity

Description Indomethacin is a nonselective COX1 and COX2 inhibitor with IC50 of 0.1 μg/mL and 5 μg/mL, respectively, used to reduce fever, pain, stiffness, and swelling.
Targets
COX1 [6] COX-2 [6]
0.28 μM 14 μM
In vitro

Indomethacin inhibits transcription of a beta-catenin/TCF-responsive reporter gene in a dose dependent manner. Indomethacin also downregulates the beta-catenin/TCF transcriptional target cyclin D1. Indomethacin attenuates the transcription of beta-catenin/TCF-responsive genes, by modulating TCF activity without disrupting beta-catenin/TCF complex formation. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HeLa cells NFO4VI1HfW6ldHnvckBie3OjeR?= NH\2UI8zOCCvaX7z MoCwRYdwdmm|dDDhZ5Rqfmm2eTDheEBidmS{b3flckBz\WOncITvdkApfW6tbn;3ckBwemmpaX6pJIV5eHKnc4Pl[EBqdiCKZVzhJINmdGy|IHPvMYV5eHKnc4PpcochWFODLTjBVmUqPC2OdXOxN{Bie3Onc4Pl[EBieyCrbnT1Z5Rqd25ib3[gSGhVNWmwZIXj[YQhdHWlaX\ldoF{\SCjY4Tpeol1gSCjZoTldkAzOCCvaX7zJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygSWM2OD1yLkG0JI5O NFLJRWwzOzR|MkC5OS=>
J774 cells MYLGeY5kfGmxbjDhd5NigQ>? MVrJckB3cXS{bzDpcohq[mm2b4L5JIFkfGm4aYT5JIFo[Wmwc4SgVJJwe3SjZ3zhcoRqdiCJL1igd5lvfGijc3WgNUBqdiCvdYLpcoUhUjd5NDDj[YxteyxiSVO1NF0zKG6P M3yy[FE2QDV5MUS5
U-937 cells MV7GeY5kfGmxbjDhd5NigQ>? MkTlTY4hfmm2cn:gbY5pcWKrdH;yfUBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGDyc5N1[WeuYX7kbY4hTy:KIIP5cpRp[XOnIEGgLGNQYC1zKTDpckBWNTl|NzDj[YxteyxiSVO1NF0zKG6P NXrrNnFxOTJ6N{e1PFQ>
rat synovial cells Moq1SpVv[3Srb36gZZN{[Xl? M4[zVWlvKH[rdILvJIlvcGmkaYTvdpkh\W[oZXP0JI9vKHC{b3T1Z5Rqd25ib3[gdJJwe3SjZ3zhcoRqdiCHMjCoVGdGOiliaX6gdoF1KHO7bn;2bYFtKGOnbHzzMEBKSzVyPUKuPUBvVQ>? MY[xNFgzOTdzNh?=
143982 cells M2jVb2Z2dmO2aX;uJIF{e2G7 MmHWTY4hfmm2cn:gbY5pcWKrdH;yfUBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGDyc5N1[WeuYX7kbY4hTy:KIIP5cpRp[XOnIEKgLGNQYC1{KTDpckAyPDN7OEKgZ4VtdHNuIFnDOVA:QSCwTR?= MoPrNVI5Pzd3OES=
CHO cells NVO3N5pLTnWwY4Tpc44h[XO|YYm= M3vBPGlvKH[rdILvJIlvcGmkaYTvdpkheG:2ZX7jfUBi\2GrboP0JIh2dWGwIFPPXE0yKGmwIIP0ZYJtgSC2cnHud4Zm[3SnZDDjbIlv\XOnIHjhcZN1\XJib4\hdpkhMEOKTzmgZ4VtdHNuIFnDOVA:OThibl2= M4m3dlExPTd4Nki0
RBL-1 cell line NEW0T2tHfW6ldHnvckBie3OjeR?= NHXzPHlKdmirYnn0bY9vKG:oIGDyc5N1[WeuYX7kbY4hTy:KIIP5cpRp[XOnIHnuJIlvfGGldDDSRmwuOSClZXzsJIxqdmVuIFnDOVA:OC53IN88US=> NFzPRlIzOTF3NUi2
SF-9 insect cells M13sVGZ2dmO2aX;uJIF{e2G7 MVeyNEBOcW6| NUfYd5ZUUW6qaXLpeIlwdiCxZjDoeY1idiCFT2iyJIV5eHKnc4Pl[EBqdiCVRj25JIlve2WldDDj[YxteyCjc4Pld5Nm\CCjczDjc453\XK|aX;uJI9nKFtzNFPdMYFz[WOqaXTvcolkKGGlaXSgeI8hYzF2Q22tdJJwe3SjZ3zhcoRqdnNicILlbY5kfWKjdHXkJIZweiB{MDDtbY5{KGK7IITobY4udGG7ZYKgZ4hzd22jdH;ndoFxcHliYX7hcJl{cXNuIFnDOVA:OC55NTFOwG0> NVi5NHc4OjF3MkS1PFc>
mouse RAW264.7 cells MoHjSpVv[3Srb36gZZN{[Xl? MXyyOEBp MnjCRY51cWmwZnzhcY1ifG:{eTDhZ5Rqfmm2eTDpckBud3W|ZTDSRXczPjRwNzDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKEySUz3pcoR2[2WmIF7PJJBzd2S3Y4Tpc44h[W[2ZYKgNlQhcHK|IHL5JGdzcWW|czDhd5NigSxiSVO1NF0yNjJ3IN88US=> NUf6VJc6OjR6ME[zNVA>
mouse BV2 cells NEPqdG5HfW6ldHnvckBie3OjeR?= MmGxNlQhcA>? NY\OUoJvSW62aXnu[oxidW2jdH;yfUBi[3Srdnn0fUBqdiCvb4Xz[UBDXjJiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBNWFNvaX7keYNm\CCQTzDwdo9lfWO2aX;uJIFnfGW{IEK0JIhzeyCkeTDHdolme3NiYYPzZZktKEmFNUC9O{4yKM7:TR?= M3XteFI1ODR{MEC3
human 2008 cells MWDGeY5kfGmxbjDhd5NigQ>? NGOwcWxKdmirYnn0bY9vKG:oIHj1cYFvKE2UUEGgbY4hcHWvYX6gNlAxQCClZXzsd{whUUN3ME2xNkDPxE1? NUCyc41ZOTh5MEe4PFQ>
A549 cells  NHzkcpVHfW6ldHnvckBie3OjeR?= MnL0NVAh|ryP MYXJcohq[mm2aX;uJI9nKG2SR1XTNUBqdiCqdX3hckBCPTR7IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhUUxvMT3i[ZRiNWmwZIXj[YQhWEeHMjDmc5Ju[XSrb36gZZQhOTBidV2gZpkh[2WubD3pcpRi[3RiYYPzZZk> NFSweWYyQTB3M{e1NS=>
HepG2 cells NH[wbYhHfW6ldHnvckBie3OjeR?= NVfU[HU1OTByIN88US=> M4fLRWlvcGmkaYTpc44hd2ZiTWLQNU1u\WSrYYTl[EBld3ixcoXibYNqdiCnZn\seZghcW5iZH;4c5J2[mmlaX6gdoV{cXO2YX70JIh2dWGwIFjldGczKGOnbHzzJIF1KDFyMDD1UUBjgSCobH;3JIN6fG:vZYTyfS=> M3XvSFIxPDV3NUe4
THP1 cells NEPEXVBHfW6ldHnvckBie3OjeR?= M13oZ|ExOCEQvF2= MVmzNEBucW6| NVvCO4JQUXK{ZY\ldpNq[mynIHnubIljcXSrb36gc4YhS0:[LUGgbY4hcHWvYX6gWGhROSClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJIFz[WOqaXTvcolkKGGlaXStbY5lfWOnZDDUXGIzKG[xcn3heIlwdiCjdDCxNFAhfU1iaX7jeYJifGWmIH\vdkA{OCCvaX7zJIZwdGyxd3XkJIJ6KGOxbYDveY5lKHejc3jveZQhdWWjc4Xy[YQhOzBibXnud{Bxd3O2IHHyZYNpcWSxbnnjJIFkcWRiY3jhcIxmdmenIHL5JJJi\GmxaX3teY5w[XO|YYm= NHX2WpYzOzZ3MUO1PS=>
MDA-MB-231 cells NHG0d|VHfW6ldHnvckBie3OjeR?= NIrKTGsyODBizszN M1nxcFMxKG2rboO= NEjF[odKenKndnXyd4ljdGViaX7obYJqfGmxbjDv[kBEV1hvMTDpckBpfW2jbjDNSGEuVUJvMkOxJINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiYYLhZ4hq\G:waXOgZYNq\C2rbnT1Z4VlKFCJRUKg[o9zdWG2aX;uJIF1KDFyMDD1UUBqdmO3YnH0[YQh\m:{IEOwJI1qdnNiZn;scI94\WRiYomgZ49ueG:3bnSge4F{cG:3dDDt[YF{fXKnZDCzNEBucW6|IIDvd5Qh[XKjY3jp[I9vcWNiYXPp[EBkcGGubHXu[4Uh[nlicnHkbY9qdW23bn;hd5NigQ>? MYeyN|Y2OTN3OR?=
LNCAP cells NETQ[m1HfW6ldHnvckBie3OjeR?= NVruU5lXOzBizszN MUW0PEBp MlfjTY5pcWKrdHnvckBw\iCDS2KxR|MhMHWwa37ve44hd3KrZ3nuLU1u\WSrYYTl[EB1\XO2b4P0[ZJwdmVvMUfi[ZRiNWeudXP1do9vcWSnIH\vdo1ifGmxbjDlfJBz\XO|ZXSgbY4hcHWvYX6gUG5ESVBiY3XscJMh[XRiM{CgeW0h[W[2ZYKgOFghcHK|IHnuJJBz\XOnbnPlJI9nKDRvYX7kdo9{fGWwZT2zMFE4NWSrb37l NULtbmhNOjN2M{KwPVU>
mouse BV2 cells MV;DfZRwfG:6aXPpeJkh[XO|YYm= MoDKNlQhcA>? NVfQSI93S3m2b4TvfIlkcXS7IHHnZYlve3RibX;1d4UhSlZ{IHPlcIx{KGG|c3Xzd4VlKGG|IH3hfIlufW1ibn;uMZRwgGmlIHPvcoNmdnS{YYTpc44h[W[2ZYKgNlQhcHK|IHL5JG1VXCCjc4PhfS=> NH;M[YUzOzdzMUmxOy=>

... Click to View More Cell Line Experimental Data

In vivo Indomethacin increases BrdU+ cells of all lineages and reduces microglial/monocyte activation in rats after focal cerebral ischemia. [2] Indomethacin (7.5 mg/kg, one injection) produces acute injury and inflammation in the distal jejunum and proximal ileum that are maximal at three days and completely resolves within one week. Indomethacin (two daily subcutaneous injections) produces a more extensive and chronic inflammation that lasts in an active form in more than 75% of the rats for at least two weeks. [3] Indomethacin (4ppm) reduces tumor yield by 78% in SKH:HR-1-hrBr hairless mice. [4] Indomethacin (20 mg/kg, orally) increases the total area of gastric erosions and concentration of lipid peroxides in the gastric mucosa of rats. Indomethacin increases the alpha-tocopherol:total cholesterol ratio in serum. Indomethacin inhibits the increases in gastric mucosal erosions and lipid peroxides in the gastric mucosa, and the reduction of serum alpha-tocopherol. [5]

Protocol

Solubility (25°C)

In vitro DMSO 72 mg/mL (201.23 mM)
Ethanol 24 mg/mL (67.07 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+30% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.
2mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 357.79
Formula

C19H16ClNO4

CAS No. 53-86-1
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

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Dilution Calculator

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Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

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Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT02849990 Recruiting Stage III Prostate Adenocarcinoma AJCC v7|Stage III Prostate Cancer AJCC v7|Stage IV Prostate Adenocarcinoma AJCC v7|Stage IV Prostate Cancer AJCC v7 University of Washington|Janssen Scientific Affairs LLC|National Cancer Institute (NCI) March 9 2017 Phase 2
NCT02950259 Active not recruiting Breast Neoplasm|Breast Neoplasm Male|Triple Negative Breast Cancer Providence Health & Services|IRX Therapeutics February 9 2017 Phase 1
NCT03267680 Recruiting Cervical Squamous Cell Carcinoma In Situ|Vulvar High Grade Squamous Intraepithelial Lesion University of Southern California|National Cancer Institute (NCI) November 8 2017 Phase 2
NCT03261635 Completed Macular Edema Università degli Studi di Brescia January 7 2016 Phase 4
NCT02884219 Recruiting Patent Ductus Arteriosus Radboud University|ZonMw: The Netherlands Organisation for Health Research and Development December 23 2016 Not Applicable
NCT02964403 Not yet recruiting Pancreatitis Acute First Affiliated Hospital Xi''an Jiaotong University December 2016 Phase 4

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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COX Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID