Indomethacin

Catalog No.S1723

Indomethacin  Chemical Structure

Molecular Weight(MW): 357.79

Indomethacin is a nonselective COX1 and COX2 inhibitor with IC50 of 0.1 μg/mL and 5 μg/mL, respectively, used to reduce fever, pain, stiffness, and swelling.

Size Price Stock Quantity  
In DMSO USD 130 In stock
USD 97 In stock
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1 Customer Review

  • Indicated PN and MES spheres were shown expressing GFP under the control of a 7 × TCF/LEF optimal promoter cassette (7 × TOP) and constitutively expressed nuclear mCherry. PN 84 and MES 1123 were treated with Wnt3a (200 ng/ml), indomethacin (Indo; 20 μM), or a control (vehicle). Scale bar, 100 μm. Bar graph, quantification of fluorescent signal of GFP (TOP-GFP) versus mCherry (PGK-H2BmCherry). The percentage of GFP- and mCherry-positive spheres was determined by FACS.

    Nat Commun, 2016, 7:12885. Indomethacin purchased from Selleck.

Purity & Quality Control

Choose Selective COX Inhibitors

Biological Activity

Description Indomethacin is a nonselective COX1 and COX2 inhibitor with IC50 of 0.1 μg/mL and 5 μg/mL, respectively, used to reduce fever, pain, stiffness, and swelling.
Targets
COX1 [6] COX-2 [6]
0.28 μM 14 μM
In vitro

Indomethacin inhibits transcription of a beta-catenin/TCF-responsive reporter gene in a dose dependent manner. Indomethacin also downregulates the beta-catenin/TCF transcriptional target cyclin D1. Indomethacin attenuates the transcription of beta-catenin/TCF-responsive genes, by modulating TCF activity without disrupting beta-catenin/TCF complex formation. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HeLa cells MYfGeY5kfGmxbjDhd5NigQ>? M{fYW|IxKG2rboO= MVHB[49vcXO2IHHjeIl3cXS7IHH0JIFv\HKxZ3XuJJJm[2WydH;yJEh2dmuwb4fuJI9zcWerbjmg[ZhxemW|c3XkJIlvKEinTHGgZ4VtdHNiY3:t[ZhxemW|c3nu[{BRW0FvKFHSSUk1NUy3Y{GzJIF{e2W|c3XkJIF{KGmwZIXjeIlwdiCxZjDETHQucW6mdXPl[EBtfWOrZnXyZZNmKGGldHn2bZR6KGGodHXyJFIxKG2rboOgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDFR|UxRTBwMUSgcm0> M1PVZ|I{PDN{MEm1
J774 cells NWXxV3I1TnWwY4Tpc44h[XO|YYm= NVj5WmNWUW5idnn0do8hcW6qaXLpeI9zgSCjY4Tpeol1gSCjZ3HpcpN1KFC{b4P0ZYdt[W6maX6gS{9JKHO7boToZZNmKDFiaX6gcZVzcW6nIFq3O|Qh[2WubIOsJGlEPTB;MjDuUS=> Mm[wNVU5PTdzNEm=
U-937 cells MlPGSpVv[3Srb36gZZN{[Xl? NH74UXRKdiC4aYTyc{BqdmirYnn0c5J6KGGldHn2bZR6KGGpYXnud5QhcHWvYX6gVJJwe3SjZ3zhcoRqdiCJL1igd5lvfGijc3WgNUApS0:[LUGpJIlvKFVvOUO3JINmdGy|LDDJR|UxRTJibl2= NYTvUHBVOTJ6N{e1PFQ>
rat synovial cells NYfxWG0yTnWwY4Tpc44h[XO|YYm= NWDnWYl1UW5idnn0do8hcW6qaXLpeI9zgSCnZn\lZ5Qhd25icILv[JVkfGmxbjDv[kBxem:|dHHncIFv\GmwIFWyJEhRT0V{KTDpckBz[XRic4nuc5Zq[WxiY3XscJMtKEmFNUC9Nk46KG6P MWGxNFgzOTdzNh?=
143982 cells MYPGeY5kfGmxbjDhd5NigQ>? MUDJckB3cXS{bzDpcohq[mm2b4L5JIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iUILvd5Ri\2yjbnTpckBIN0hic4nueIhie2ViMjCoR29ZNTJrIHnuJFE1Ozl6MjDj[YxteyxiSVO1NF06KG6P NELHUlMyOjh5N{W4OC=>
CHO cells MYDGeY5kfGmxbjDhd5NigQ>? NGrsb4pKdiC4aYTyc{BqdmirYnn0c5J6KHCxdHXuZ5kh[WejaX7zeEBpfW2jbjDDU3guOSCrbjDzeIFjdHlidILhcpNn\WO2ZXSgZ4hqdmW|ZTDoZY1{fGW{IH;2ZZJ6KCiFSF:pJINmdGy|LDDJR|UxRTF6IH7N NHvIdFcyODV5Nk[4OC=>
RBL-1 cell line MlPGSpVv[3Srb36gZZN{[Xl? M1PSeGlvcGmkaYTpc44hd2ZiUILvd5Ri\2yjbnTpckBIN0hic4nueIhie2ViaX6gbY51[WO2IGLCUE0yKGOnbHygcIlv\SxiSVO1NF0xNjVizszN MlvUNlEyPTV6Nh?=
SF-9 insect cells M{jNb2Z2dmO2aX;uJIF{e2G7 Mn7NNlAhVWmwcx?= NF;YeVZKdmirYnn0bY9vKG:oIHj1cYFvKEORWEKg[ZhxemW|c3XkJIlvKFOILUmgbY5{\WO2IHPlcIx{KGG|c3Xzd4VlKGG|IHPvcpZmenOrb36gc4YhYzF2Q22tZZJi[2irZH;ubYMh[WOrZDD0c{BcOTSFXT3wdo9{fGGpbHHu[IlveyCycnXpcoN2[mG2ZXSg[o9zKDJyIH3pcpMh[nlidHjpck1t[XmncjDjbJJwdWG2b3fyZZBpgSCjbnHsfZNqeyxiSVO1NF0xNjd3IN88US=> NGHtPJkzOTV{NEW4Oy=>
mouse RAW264.7 cells MkLRSpVv[3Srb36gZZN{[Xl? NU\sTpBDOjRiaB?= NYf1dmdGSW62aXnu[oxidW2jdH;yfUBi[3Srdnn0fUBqdiCvb4Xz[UBTSVd{NkSuO{Bk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIFzQV{1qdmS3Y3XkJG5QKHC{b3T1Z5Rqd25iYX\0[ZIhOjRiaILzJIJ6KEe{aXXzd{Bie3OjeTygTWM2OD1zLkK1JO69VQ>? MXeyOFgxPjNzMB?=
mouse BV2 cells MojnSpVv[3Srb36gZZN{[Xl? NEDGN2QzPCCq MlPFRY51cWmwZnzhcY1ifG:{eTDhZ5Rqfmm2eTDpckBud3W|ZTDCWlIh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjDMVHMucW6mdXPl[EBPVyCycn;keYN1cW:wIHHmeIVzKDJ2IHjyd{BjgSCJcnnld5Mh[XO|YYmsJGlEPTB;Nz6xJO69VQ>? MofxNlQxPDJyMEe=
human 2008 cells MlLZSpVv[3Srb36gZZN{[Xl? NUTkdZVqUW6qaXLpeIlwdiCxZjDoeY1idiCPUmCxJIlvKGi3bXHuJFIxODhiY3XscJMtKEmFNUC9NVIh|ryP NXzDU3FUOTh5MEe4PFQ>
A549 cells  NFfPSmRHfW6ldHnvckBie3OjeR?= NIPIUGYyOCEQvF2= M3Kw[WlvcGmkaYTpc44hd2ZibWDHSXMyKGmwIHj1cYFvKEF3NEmgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCLTD2xMYJmfGFvaX7keYNm\CCSR1WyJIZwem2jdHnvckBifCBzMDD1UUBjgSClZXzsMYlvfGGldDDhd5NigQ>? M33JRVE6ODV|N{Wx
HepG2 cells NGXCenVHfW6ldHnvckBie3OjeR?= NXjjSXF4OTByIN88US=> NGXDUFdKdmirYnn0bY9vKG:oIF3SVFEudWWmaXH0[YQh\G:6b4L1ZolkcW5iZX\mcJV5KGmwIHTvfI9zfWKrY3nuJJJme2m|dHHueEBpfW2jbjDI[ZBIOiClZXzsd{BifCBzMECgeW0h[nliZnzve{BkgXSxbXX0dpk> MYSyNFQ2PTV5OB?=
THP1 cells NIrkZmZHfW6ldHnvckBie3OjeR?= MWSxNFAh|ryP Mkn4N|AhdWmwcx?= NI\mbG1KenKndnXyd4ljdGViaX7obYJqfGmxbjDv[kBEV1hvMTDpckBpfW2jbjDUTHAyKGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gZZJi[2irZH;ubYMh[WOrZD3pcoR2[2WmIGTYRlIh\m:{bXH0bY9vKGG2IEGwNEB2VSCrbnP1ZoF1\WRiZn;yJFMxKG2rboOg[o9tdG:5ZXSgZpkh[2:vcH;1coQhf2G|aH;1eEBu\WG|dYLl[EA{OCCvaX7zJJBwe3RiYYLhZ4hq\G:waXOgZYNq\CClaHHscIVv\2ViYomgdoFlcW:rbX31co9ie3OjeR?= NF\yPWszOzZ3MUO1PS=>
MDA-MB-231 cells M3XFOmZ2dmO2aX;uJIF{e2G7 NGna[FIyODBizszN MVezNEBucW6| Ml\4TZJz\X[ncoPpZoxmKGmwaHnibZRqd25ib3[gR29ZNTFiaX6gbJVu[W5iTVTBMW1DNTJ|MTDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKGG{YXPobYRwdmmlIHHjbYQucW6mdXPl[EBRT0V{IH\vdo1ifGmxbjDheEAyODBidV2gbY5kfWKjdHXkJIZweiB|MDDtbY5{KG[xbHzve4VlKGK7IHPvcZBwfW6mIIfhd4hwfXRibXXhd5Vz\WRiM{CgcYlveyCyb4P0JIFz[WOqaXTvcolkKGGlaXSgZ4hidGynbnflJIJ6KHKjZHnvbY1ufW6xYYPzZZk> MY[yN|Y2OTN3OR?=
LNCAP cells NX3FNGZxTnWwY4Tpc44h[XO|YYm= M4TNcVMxKM7:TR?= NF;RNZc1QCCq Mn;4TY5pcWKrdHnvckBw\iCDS2KxR|MhMHWwa37ve44hd3KrZ3nuLU1u\WSrYYTl[EB1\XO2b4P0[ZJwdmVvMUfi[ZRiNWeudXP1do9vcWSnIH\vdo1ifGmxbjDlfJBz\XO|ZXSgbY4hcHWvYX6gUG5ESVBiY3XscJMh[XRiM{CgeW0h[W[2ZYKgOFghcHK|IHnuJJBz\XOnbnPlJI9nKDRvYX7kdo9{fGWwZT2zMFE4NWSrb37l MVSyN|Q{OjB7NR?=
mouse BV2 cells NXL0NllsS3m2b4TvfIlkcXS7IHHzd4F6 NF7QdlkzPCCq NXPleIlZS3m2b4TvfIlkcXS7IHHnZYlve3RibX;1d4UhSlZ{IHPlcIx{KGG|c3Xzd4VlKGG|IH3hfIlufW1ibn;uMZRwgGmlIHPvcoNmdnS{YYTpc44h[W[2ZYKgNlQhcHK|IHL5JG1VXCCjc4PhfS=> NH;BRpUzOzdzMUmxOy=>

... Click to View More Cell Line Experimental Data

In vivo Indomethacin increases BrdU+ cells of all lineages and reduces microglial/monocyte activation in rats after focal cerebral ischemia. [2] Indomethacin (7.5 mg/kg, one injection) produces acute injury and inflammation in the distal jejunum and proximal ileum that are maximal at three days and completely resolves within one week. Indomethacin (two daily subcutaneous injections) produces a more extensive and chronic inflammation that lasts in an active form in more than 75% of the rats for at least two weeks. [3] Indomethacin (4ppm) reduces tumor yield by 78% in SKH:HR-1-hrBr hairless mice. [4] Indomethacin (20 mg/kg, orally) increases the total area of gastric erosions and concentration of lipid peroxides in the gastric mucosa of rats. Indomethacin increases the alpha-tocopherol:total cholesterol ratio in serum. Indomethacin inhibits the increases in gastric mucosal erosions and lipid peroxides in the gastric mucosa, and the reduction of serum alpha-tocopherol. [5]

Protocol

Solubility (25°C)

In vitro DMSO 72 mg/mL (201.23 mM)
Ethanol 24 mg/mL (67.07 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 357.79
Formula

C19H16ClNO4

CAS No. 53-86-1
Storage powder
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

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The Serial Dilution Calculator Equation

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Molecular Weight Calculator

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Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

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Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT02935205 Not yet recruiting Metastatic Prostate Carcinoma|Recurrent Prostate Carcinoma|Stage IV Prostate Cancer University of California, Davis|National Cancer Institute (NCI) January 2017 Phase 1|Phase 2
NCT02964403 Not yet recruiting Pancreatitis, Acute First Affiliated Hospital Xian Jiaotong University December 2016 Phase 4
NCT02947932 Not yet recruiting Pancreatitis, Acute|Gastrointestinal Disease First Affiliated Hospital Xian Jiaotong University December 2016 Phase 4
NCT01869361 Not yet recruiting Preterm Labor MetroHealth Medical Center November 2016 --
NCT02950259 Not yet recruiting Breast Neoplasm|Breast Neoplasm, Male Providence Health & Services|IRX Therapeutics November 2016 Phase 1
NCT02849990 Not yet recruiting Stage III Prostate Adenocarcinoma|Stage III Prostate Cancer|Stage IV Prostate Adenocarcinoma|Stage IV Prostate Cancer University of Washington|National Cancer Institute (NCI)|Janssen Scientific Affairs, LLC October 2016 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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COX Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID