Indomethacin

Catalog No.S1723

Indomethacin  Chemical Structure

Molecular Weight(MW): 357.79

Indomethacin is a nonselective COX1 and COX2 inhibitor with IC50 of 0.1 μg/mL and 5 μg/mL, respectively, used to reduce fever, pain, stiffness, and swelling.

Size Price Stock Quantity  
In DMSO USD 130 In stock
USD 97 In stock
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1 Customer Review

  • Indicated PN and MES spheres were shown expressing GFP under the control of a 7 × TCF/LEF optimal promoter cassette (7 × TOP) and constitutively expressed nuclear mCherry. PN 84 and MES 1123 were treated with Wnt3a (200 ng/ml), indomethacin (Indo; 20 μM), or a control (vehicle). Scale bar, 100 μm. Bar graph, quantification of fluorescent signal of GFP (TOP-GFP) versus mCherry (PGK-H2BmCherry). The percentage of GFP- and mCherry-positive spheres was determined by FACS.

    Nat Commun, 2016, 7:12885. Indomethacin purchased from Selleck.

Purity & Quality Control

Choose Selective COX Inhibitors

Biological Activity

Description Indomethacin is a nonselective COX1 and COX2 inhibitor with IC50 of 0.1 μg/mL and 5 μg/mL, respectively, used to reduce fever, pain, stiffness, and swelling.
Targets
COX1 [6] COX-2 [6]
0.28 μM 14 μM
In vitro

Indomethacin inhibits transcription of a beta-catenin/TCF-responsive reporter gene in a dose dependent manner. Indomethacin also downregulates the beta-catenin/TCF transcriptional target cyclin D1. Indomethacin attenuates the transcription of beta-catenin/TCF-responsive genes, by modulating TCF activity without disrupting beta-catenin/TCF complex formation. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HeLa cells MWPGeY5kfGmxbjDhd5NigQ>? M2T4cVIxKG2rboO= M1\5SGFod26rc4SgZYN1cX[rdImgZZQh[W6mcn;n[Y4hemWlZYD0c5IhMHWwa37ve44hd3KrZ3nuLUBmgHC{ZYPz[YQhcW5iSHXMZUBk\WyuczDjc{1mgHC{ZYPzbY5oKFCVQT2oRXJGMTRvTIXjNVMh[XO|ZYPz[YQh[XNiaX7keYN1cW:wIH;mJGRJXC2rbnT1Z4VlKGy3Y3nm[ZJie2ViYXP0bZZqfHliYX\0[ZIhOjBibXnud{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYmsJGVEPTB;MD6xOEBvVQ>? MVqyN|Q{OjB7NR?=
J774 cells M3PCfGZ2dmO2aX;uJIF{e2G7 NFrme3RKdiC4aYTyc{BqdmirYnn0c5J6KGGldHn2bZR6KGGpYXnud5QhWHKxc4Th[4xidmSrbjDHM2ghe3mwdHjhd4UhOSCrbjDteZJqdmViSke3OEBk\WyuczygTWM2OD1{IH7N NV;pPVZWOTV6NUexOFk>
U-937 cells MWLGeY5kfGmxbjDhd5NigQ>? MnvUTY4hfmm2cn:gbY5pcWKrdH;yfUBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGDyc5N1[WeuYX7kbY4hTy:KIIP5cpRp[XOnIEGgLGNQYC1zKTDpckBWNTl|NzDj[YxteyxiSVO1NF0zKG6P M2PwUVEzQDd5NUi0
rat synovial cells NX7MflJlTnWwY4Tpc44h[XO|YYm= MnTYTY4hfmm2cn:gbY5pcWKrdH;yfUBm\m[nY4Sgc44heHKxZIXjeIlwdiCxZjDwdo9{fGGpbHHu[IlvKEV{IDjQS2UzMSCrbjDyZZQhe3mwb4\pZYwh[2WubIOsJGlEPTB;Mj65JI5O MVGxNFgzOTdzNh?=
143982 cells MYHGeY5kfGmxbjDhd5NigQ>? NGTjS|VKdiC4aYTyc{BqdmirYnn0c5J6KGGldHn2bZR6KGGpYXnud5QhcHWvYX6gVJJwe3SjZ3zhcoRqdiCJL1igd5lvfGijc3WgNkApS0:[LUKpJIlvKDF2M{m4NkBk\WyuczygTWM2OD17IH7N NX3qfJczOTJ6N{e1PFQ>
CHO cells NG\UbYFHfW6ldHnvckBie3OjeR?= MX3JckB3cXS{bzDpcohq[mm2b4L5JJBwfGWwY4mgZYdicW6|dDDoeY1idiCFT2itNUBqdiC|dHHicJkhfHKjboPm[YN1\WRiY3jpcoV{\SCqYX3zeIVzKG:4YYL5JEhEUE9rIHPlcIx{NCCLQ{WwQVE5KG6P M1v0WFExPTd4Nki0
RBL-1 cell line NIjJPYVHfW6ldHnvckBie3OjeR?= NGD5TJRKdmirYnn0bY9vKG:oIGDyc5N1[WeuYX7kbY4hTy:KIIP5cpRp[XOnIHnuJIlvfGGldDDSRmwuOSClZXzsJIxqdmVuIFnDOVA:OC53IN88US=> MojRNlEyPTV6Nh?=
SF-9 insect cells M2PRTWZ2dmO2aX;uJIF{e2G7 MXqyNEBOcW6| MlrxTY5pcWKrdHnvckBw\iCqdX3hckBEV1h{IHX4dJJme3OnZDDpckBUTi17IHnud4VkfCClZXzsd{Bie3Onc4Pl[EBieyClb372[ZJ{cW:wIH;mJHsyPEOfLXHyZYNpcWSxbnnjJIFkcWRidH:gX|E1S11vcILvd5Ri\2yjbnTpcpMheHKnaX7jeYJifGWmIH\vdkAzOCCvaX7zJIJ6KHSqaX6tcIF6\XJiY3jyc41ifG:pcnHwbJkh[W6jbInzbZMtKEmFNUC9NE44PSEQvF2= Ml\UNlE2OjR3OEe=
mouse RAW264.7 cells MoLWSpVv[3Srb36gZZN{[Xl? MX2yOEBp MVzBcpRqcW6obHHtcYF1d3K7IHHjeIl3cXS7IHnuJI1wfXOnIGLBW|I3PC55IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhVFCVLXnu[JVk\WRiTl:gdJJw\HWldHnvckBi\nSncjCyOEBpenNiYomgS5Jq\XO|IHHzd4F6NCCLQ{WwQVEvOjVizszN MWmyOFgxPjNzMB?=
mouse BV2 cells MX7GeY5kfGmxbjDhd5NigQ>? NHzyN4ozPCCq NXvlV5N2SW62aXnu[oxidW2jdH;yfUBi[3Srdnn0fUBqdiCvb4Xz[UBDXjJiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBNWFNvaX7keYNm\CCQTzDwdo9lfWO2aX;uJIFnfGW{IEK0JIhzeyCkeTDHdolme3NiYYPzZZktKEmFNUC9O{4yKM7:TR?= MXuyOFA1OjByNx?=
human 2008 cells M2PDTWZ2dmO2aX;uJIF{e2G7 NX\wc29JUW6qaXLpeIlwdiCxZjDoeY1idiCPUmCxJIlvKGi3bXHuJFIxODhiY3XscJMtKEmFNUC9NVIh|ryP NYXOXoRmOTh5MEe4PFQ>
A549 cells  MlrUSpVv[3Srb36gZZN{[Xl? NFSxc40yOCEQvF2= MlizTY5pcWKrdHnvckBw\iCvUFfFV|EhcW5iaIXtZY4hSTV2OTDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKEmOLUGtZoV1[S2rbnT1Z4VlKFCJRUKg[o9zdWG2aX;uJIF1KDFyIIXNJIJ6KGOnbHytbY51[WO2IHHzd4F6 NHTJVZcyQTB3M{e1NS=>
HepG2 cells NFnjRm1HfW6ldHnvckBie3OjeR?= MmrjNVAxKM7:TR?= MmnETY5pcWKrdHnvckBw\iCPUmCxMY1m\GmjdHXkJIRwgG:{dXLpZ4lvKGWoZnz1fEBqdiCmb4jvdpVjcWOrbjDy[ZNqe3SjboSgbJVu[W5iSHXwS|Ih[2WubIOgZZQhOTByIIXNJIJ6KG[ub4egZ5l1d22ndIL5 M1nlVlIxPDV3NUe4
THP1 cells NU[zfGJkTnWwY4Tpc44h[XO|YYm= NGTLW|QyODBizszN NYGxPVN3OzBibXnudy=> MnW2TZJz\X[ncoPpZoxmKGmwaHnibZRqd25ib3[gR29ZNTFiaX6gbJVu[W5iVFjQNUBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIHHyZYNpcWSxbnnjJIFkcWRvaX7keYNm\CCWWFKyJIZwem2jdHnvckBifCBzMECgeW0hcW6ldXLheIVlKG[xcjCzNEBucW6|IH\vcIxwf2WmIHL5JINwdXCxdX7kJJdie2ixdYSgcYVie3W{ZXSgN|AhdWmwczDwc5N1KGG{YXPobYRwdmmlIHHjbYQh[2ijbHzlcodmKGK7IILh[IlwcW2vdX7vZZN{[Xl? MYGyN|Y2OTN3OR?=
MDA-MB-231 cells M4T6[mZ2dmO2aX;uJIF{e2G7 MmL2NVAxKM7:TR?= NIPwTmI{OCCvaX7z NHPQbZBKenKndnXyd4ljdGViaX7obYJqfGmxbjDv[kBEV1hvMTDpckBpfW2jbjDNSGEuVUJvMkOxJINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiYYLhZ4hq\G:waXOgZYNq\C2rbnT1Z4VlKFCJRUKg[o9zdWG2aX;uJIF1KDFyMDD1UUBqdmO3YnH0[YQh\m:{IEOwJI1qdnNiZn;scI94\WRiYomgZ49ueG:3bnSge4F{cG:3dDDt[YF{fXKnZDCzNEBucW6|IIDvd5Qh[XKjY3jp[I9vcWNiYXPp[EBkcGGubHXu[4Uh[nlicnHkbY9qdW23bn;hd5NigQ>? M1q5TFI{PjVzM{W5
LNCAP cells M1PYT2Z2dmO2aX;uJIF{e2G7 MojZN|Ah|ryP NWXFfI4xPDhiaB?= MXLJcohq[mm2aX;uJI9nKEGNUkHDN{ApfW6tbn;3ckBwemmpaX6pMY1m\GmjdHXkJJRme3Sxc4Tldo9v\S1zN3LleIEu\2y3Y4Xyc45q\GViZn;ycYF1cW:wIHX4dJJme3OnZDDpckBpfW2jbjDMUmNCWCClZXzsd{BifCB|MDD1UUBi\nSncjC0PEBpenNiaX6gdJJme2WwY3Wgc4YhPC2jbnTyc5N1\W6nLUOsNVcu\GmxbnW= MlTYNlM1OzJyOUW=
mouse BV2 cells NGjqXGtEgXSxdH;4bYNqfHliYYPzZZk> MnzINlQhcA>? MmXQR5l1d3SxeHnjbZR6KGGpYXnud5QhdW:3c3WgRnYzKGOnbHzzJIF{e2W|c3XkJIF{KG2jeHnteY0hdm:wLYTvfIlkKGOxbnPlcpRz[XSrb36gZYZ1\XJiMkSgbJJ{KGK7IF3UWEBie3OjeR?= Mo\3NlM4OTF7MUe=

... Click to View More Cell Line Experimental Data

In vivo Indomethacin increases BrdU+ cells of all lineages and reduces microglial/monocyte activation in rats after focal cerebral ischemia. [2] Indomethacin (7.5 mg/kg, one injection) produces acute injury and inflammation in the distal jejunum and proximal ileum that are maximal at three days and completely resolves within one week. Indomethacin (two daily subcutaneous injections) produces a more extensive and chronic inflammation that lasts in an active form in more than 75% of the rats for at least two weeks. [3] Indomethacin (4ppm) reduces tumor yield by 78% in SKH:HR-1-hrBr hairless mice. [4] Indomethacin (20 mg/kg, orally) increases the total area of gastric erosions and concentration of lipid peroxides in the gastric mucosa of rats. Indomethacin increases the alpha-tocopherol:total cholesterol ratio in serum. Indomethacin inhibits the increases in gastric mucosal erosions and lipid peroxides in the gastric mucosa, and the reduction of serum alpha-tocopherol. [5]

Protocol

Solubility (25°C)

In vitro DMSO 72 mg/mL (201.23 mM)
Ethanol 24 mg/mL (67.07 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 357.79
Formula

C19H16ClNO4

CAS No. 53-86-1
Storage powder
Synonyms N/A

Bio Calculators

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Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT02935205 Not yet recruiting Metastatic Prostate Carcinoma|Recurrent Prostate Carcinoma|Stage IV Prostate Cancer University of California, Davis|National Cancer Institute (NCI) January 2017 Phase 1|Phase 2
NCT02964403 Not yet recruiting Pancreatitis, Acute First Affiliated Hospital Xian Jiaotong University December 2016 Phase 4
NCT02947932 Not yet recruiting Pancreatitis, Acute|Gastrointestinal Disease First Affiliated Hospital Xian Jiaotong University December 2016 Phase 4
NCT01869361 Not yet recruiting Preterm Labor MetroHealth Medical Center November 2016 --
NCT02950259 Not yet recruiting Breast Neoplasm|Breast Neoplasm, Male Providence Health & Services|IRX Therapeutics November 2016 Phase 1
NCT02849990 Not yet recruiting Stage III Prostate Adenocarcinoma|Stage III Prostate Cancer|Stage IV Prostate Adenocarcinoma|Stage IV Prostate Cancer University of Washington|National Cancer Institute (NCI)|Janssen Scientific Affairs, LLC October 2016 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID