Name: Z-VAD(OH)-FMK
Brand: Selleck Chemicals
SKU: S8102
Availability: InStock
Rating: 5 (30 reviews)

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Quality Control

Batch: Purity: 99.74%

99.74

Related Targets	Bcl-2 PD-1/PD-L1 Ferroptosis p53 Apoptosis related Synthetic Lethality STAT TNF-alpha Ras KRas
Other Caspase Inhibitors	Emricasan (IDN-6556) Z-VAD-FMK Q-VD-Oph Z-DEVD-FMK Belnacasan (VX-765) Z-IETD-FMK Ac-DEVD-CHO Z-LEHD-FMK TFA PAC-1 Z-YVAD-FMK

Cell Culture, Treatment & Working Concentration

Cell Lines	Assay Type	Activity Description	PMID
SF188	Apoptosis assay	Inhibition of 20S-protopanaxadiol-induced activation of caspases in human SF188 cells	17261067
SF188	Apoptosis assay	Inhibition of TNF-alpha-induced apoptosis in human SF188 cells	17261067
SF188	Apoptosis assay	Inhibition of cycloheximide-induced apoptosis in human SF188 cells	17261067
Click to View More Cell Line Experimental Data

Solubility

In vitro
Batch:

DMSO : 90 mg/mL (198.47 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 90 mg/mL

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	2%DMSO 1 40%PEG300 2 5%Tween80 3 53%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.200mg/ml (0.44mM)	Taking the 1 mL working solution as an example, add 20 μL of clarified DMSO stock solution of 10 mg/ml to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify it; then continue to add 530 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

%

% Tween 80

% ddH₂O

% DMSO

+

%

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	453.46	Formula	C₂₁H₂₈FN₃O₇	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	161401-82-7	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	N/A			SMILES	CC(C)C(C(=O)NC(C)C(=O)NC(CC(=O)O)C(=O)CF)NC(=O)OCC1=CC=CC=C1

Read more about storage stability stock solution CAS number SMILES

Mechanism of Action

Targets/IC₅₀/Ki	Caspase
In vitro	Z-VAD(OH)-FMK is the non-methylated form of Z-VAD-FMK, and useful in studies including recombinant, or purified enzymes.
In vivo	Z-VAD-FMK is a widely used broad-spectrum caspase inhibitor that protects muscle from compression-induced damage and restores muscle function.
References	[1] https://pubmed.ncbi.nlm.nih.gov/9829999/ [2] https://pubmed.ncbi.nlm.nih.gov/33613115/ [3] https://pubmed.ncbi.nlm.nih.gov/25737433/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2026-05-20)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT07598032	NOT_YET_RECRUITING	Prostate Cancer (Adenocarcinoma); Vitamin D on Tumor Response and Inflammatory Markers	University of Ilorin Teaching Hospital	2026-06-01	PHASE3
NCT07495605	NOT_YET_RECRUITING	Intensity of Postoperative Pain, Bacterial Load Reduction and Periapical MMP-9 Levels in Patients With Necrotic Pulp	Cairo University	2026-03-22
NCT05644522	RECRUITING	Cerebrovascular Accident; Post-polio Syndrome; Spinal Cord Injuries; Multiple Sclerosis; Muscular Dystrophy; Paralysis	Shirley Ryan AbilityLab	2024-03-01
NCT07661329	RECRUITING	Primary Open Angle Glaucoma (POAG); Ocular Hypertension (OH)	YS Life Science Co., Ltd.	2026-06-02	PHASE3
NCT07641933	RECRUITING	Intervention Studies; ASA Physical Status I; Intracanal Medication; Periapical Abscess; Retreatment	Ain Shams University	2026-06-01	PHASE1
NCT06791044	RECRUITING	Vitamin D Insufficiency	Dionyssiotis, Yannis, M.D.	2025-09-01

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Z-VAD(OH)-FMK Caspase Inhibitor

Chemical Structure

Molecular Weight: 453.46