Home Metabolism Vitamin chemical Thiamine HCl (Vitamin B1)

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Thiamine HCl (Vitamin B1) Vitamin chemical

Cat.No.S3211

Thiamine HCl (Vitamin B1) is a water-soluble vitamin of the B complex, and its phosphate derivatives are involved in many cellular processes.
Thiamine HCl (Vitamin B1) Vitamin chemical Chemical Structure

Chemical Structure

Molecular Weight: 337.27

Jump to Mechanism of Action Solubility Chemical Information, Storage & Stability

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Chemical Information, Storage & Stability

Molecular Weight 337.27 Formula

C12 H17N4OS.HCl

 Storage (From the date of receipt)
CAS No. 67-03-8 Download SDF Storage of Stock Solutions

Synonyms N/A Smiles CC1=C(SC=[N+]1CC2=CN=C(N=C2N)C)CCO.Cl.[Cl-]

Solubility

In vitro
Batch:

Water : 67 mg/mL

DMSO : Insoluble ( Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : Insoluble

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Mechanism of Action

In vitro
Thiamine HCl (Vitamin B1) (50 mM), in addition to its nutritional value, induces systemic acquired resistance (SAR) in rice, tobacco, cucumber, and Arabidopsis. It also confers resistance to fungal, bacterial, and viral infections in treated rice, Arabidopsis (Arabidopsis thaliana), and vegetable crop plants. This compound induces the transient expression of pathogenesis-related (PR) genes in rice and other plants, and potentiates stronger and more rapid PR gene expression along with up-regulation of protein kinase C activity. [1] At a concentration of 10 μM, it prevents acetaldehyde-induced inhibition of myocyte shortening in adult rat ventricular myocytes, effectively blunts the acetaldehyde-induced depression in ±dL/dt, and prevents acetaldehyde-induced shortening of time-to-peak shortening. Furthermore, it inhibits acetaldehyde-induced elevation in both protein carbonyl formation and caspase-3 activation in adult rat ventricular myocytes. [2] Its uptake is energy- and temperature-dependent, pH-sensitive, Na+-independent, and saturable at both the nanomolar (apparent Km, 30 nM) and the micromolar (apparent Km, 1.72 mM) concentration ranges in ARPE-19 cells. Uptake of this compound is adaptively regulated by extracellular substrate level via transcriptionally mediated mechanisms that involve both hTHTR-1 and hTHTR-2 in ARPE-19 cells, and is also regulated by an intracellular Ca2+-calmodulin-mediated pathway. [3] Thiamine-responsive megaloblastic anemia (TRMA) fibroblasts are rescued from death with 10 nM-30 nM of it (in the range of normal plasma thiamine concentrations). Normal fibroblasts exhibit saturable, high-affinity thiamine uptake (Km 400 nM-550 nM; Vmax 11 pmol/min/1×106 cells), while TRMA fibroblasts lack detectable high-affinity uptake. At 30 nM, the rate of its uptake by TRMA fibroblasts is 10-fold less than that of wild-type, which explains the increased apoptosis of TRMA fibroblasts. [4]
In vivo
Thiamine HCl (Vitamin B1) deficiency results in amyloid precursor protein immunoreactivity accumulated in swollen neurites within, or around lesions in rats, or in abnormal clusters in mice. [5]
References
  • https://pubmed.ncbi.nlm.nih.gov/10074490/
  • https://pubmed.ncbi.nlm.nih.gov/9261840/

Applications

Methods Biomarkers Images PMID
Western blot THICL / THICS / THI4L / THI4M / THI4S / ACT Bax / Bid / p53 / p84 Rpb1 / Fcp1 / f-Rpb3 / Tfg3 / Rpb7 / Rpb4 S3211-WB-3 18093957
Growth inhibition assay TEN1 and TEAEN1 strains hippocampal Dcx+ cells S3211-Growth-inhibition-assay-1 19028995
Glycolysis stress test Glycolysis stress S3211-Glycolysis-stress-test-1 29914147
Cell death Cell survival S3211-Cell-death-1 31193162
Western blot / RNA Blot PR-1 / rRNA PR-1 / rRNA S3211-WB-RNAB-1 15980201
Immunofluorescence Thi76KR / Thi7M399R / ThiN350K S3211-IF-1 31658248

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2024-05-22)

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT06298344 Recruiting
Congenital Heart Disease|Thiamine Deficiency|Patent Ductus Arteriosus|Ventricular Septal Defect|Atrial Septal Defect
Universitas Sumatera Utara
 May 1 2024 Early Phase 1
NCT06326996 Not yet recruiting
Coronary Heart Disease|Coronary Artery Bypass Grafting
University of California Los Angeles
 May 2024 Not Applicable
NCT06322212 Not yet recruiting
Type2diabetes
University of California Los Angeles
 April 2024 Not Applicable
NCT06323538 Not yet recruiting
Dietary Exposure|Diabetes Mellitus Type 2|Cardiovascular Diseases|Bone Loss|Sustainability|Exposure|Obesity|Vitamin Deficiency|Mineral Deficiency
German Federal Institute for Risk Assessment|Max Rubner-Institut|University of Bonn|Research Institute for Plant-Based Nutrition|University of Jena|University of Regensburg|Heidelberg University|University of Vienna
 April 1 2024 --

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