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Thiamine HCl (Vitamin B1) Vitamin chemical

Thiamine HCl (Vitamin B1), a water-soluble vitamin of the B complex, has phosphate derivatives involved in many cellular processes.

Thiamine HCl (Vitamin B1) Vitamin chemical Chemical Structure

Chemical Structure

Molecular Weight: 337.27

Purity & Quality Control

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Mechanism of Action

In vitro
In vitro Thiamine HCl (Vitamin B1) (50 mM), in addition to its nutritional value, induces systemic acquired resistance (SAR) in rice, tobacco, cucumber, and Arabidopsis. This compound-treated rice, Arabidopsis (Arabidopsis thaliana), and vegetable crop plants shows resistance to fungal, bacterial, and viral infections. It induces the transient expression of pathogenesis-related (PR) genes in rice and other plants. In addition, it potentiates stronger and more rapid PR gene expression and the up-regulation of protein kinase C activity. [1] At 10 μM, it prevents acetaldehyde-induced inhibition of myocyte shortening in adult rat ventricular myocytes, effectively blunts the acetaldehyde-induced depression in ±dL/dt, prevents acetaldehyde-induced shortening of time-to-peak shortening, and prevents acetaldehyde-induced elevation in both protein carbonyl formation and caspase-3 activation. [2] Its uptake is energy- and temperature-dependent, pH-sensitive, Na+-independent, saturable at both the nanomolar (apparent Km, 30 nM) and the micromolar (apparent Km, 1.72 mM) concentration ranges in ARPE-19 cells. Uptake of this compound is adaptively regulated by extracellular substrate level via transcriptionally mediated mechanisms that involve both hTHTR-1 and hTHTR-2 in ARPE-19 cells, it is also regulated by an intracellular Ca2+-calmodulin-mediated pathway. [3] Thiamine-responsive megaloblastic anemia (TRMA) fibroblasts are rescued from death with 10 nM-30 nM of it (in the range of normal plasma thiamine concentrations). Normal fibroblasts exhibits saturable, high-affinity thiamine uptake (Km 400 nM-550 nM; Vmax 11 pmol/min/1×106 cells), while TRMA fibroblasts lacks detectable high-affinity uptake. At 30 nM, the rate of uptake by TRMA fibroblasts is 10-fold less than that of wild-type, which explains the increased apoptosis of TRMA fibroblasts. [4]
Experimental Result Images Methods Biomarkers Images PMID
Western blot THICL / THICS / THI4L / THI4M / THI4S / ACT Bax / Bid / p53 / p84 Rpb1 / Fcp1 / f-Rpb3 / Tfg3 / Rpb7 / Rpb4 S3211-WB-3 18093957
Growth inhibition assay TEN1 and TEAEN1 strains hippocampal Dcx+ cells S3211-Growth-inhibition-assay-1 19028995
Glycolysis stress test Glycolysis stress S3211-Glycolysis-stress-test-1 29914147
Cell death Cell survival S3211-Cell-death-1 31193162
Western blot / RNA Blot PR-1 / rRNA PR-1 / rRNA S3211-WB-RNAB-1 15980201
Immunofluorescence Thi76KR / Thi7M399R / ThiN350K S3211-IF-1 31658248

In Vivo
In vivo Thiamine HCl (Vitamin B1) deficiency results in amyloid precursor protein immunoreactivity accumulated in swollen neurites within, or around lesions in rats, or in abnormal clusters in mice. [5]
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT06298344 Recruiting
Congenital Heart Disease|Thiamine Deficiency|Patent Ductus Arteriosus|Ventricular Septal Defect|Atrial Septal Defect
Universitas Sumatera Utara
 May 1 2024 Early Phase 1
NCT06326996 Not yet recruiting
Coronary Heart Disease|Coronary Artery Bypass Grafting
University of California Los Angeles
 May 2024 Not Applicable
NCT06322212 Not yet recruiting
Type2diabetes
University of California Los Angeles
 April 2024 Not Applicable
NCT06323538 Not yet recruiting
Dietary Exposure|Diabetes Mellitus Type 2|Cardiovascular Diseases|Bone Loss|Sustainability|Exposure|Obesity|Vitamin Deficiency|Mineral Deficiency
German Federal Institute for Risk Assessment|Max Rubner-Institut|University of Bonn|Research Institute for Plant-Based Nutrition|University of Jena|University of Regensburg|Heidelberg University|University of Vienna
 April 1 2024 --

References
  • https://pubmed.ncbi.nlm.nih.gov/15980201/
  • https://pubmed.ncbi.nlm.nih.gov/15304379/
  • https://pubmed.ncbi.nlm.nih.gov/17463047/
  • https://pubmed.ncbi.nlm.nih.gov/10074490/
  • https://pubmed.ncbi.nlm.nih.gov/9261840/

Chemical Information

Molecular Weight 337.27 Formula

C12 H17N4OS.HCl
CAS No. 67-03-8 SDF Download SDF
Synonyms N/A
Smiles CC1=C(SC=[N+]1CC2=CN=C(N=C2N)C)CCO.Cl.[Cl-]

Storage and Stability

Storage (From the date of receipt)

In vitro
Batch:

Water : 67 mg/mL

DMSO : Insoluble ( Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : Insoluble


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In vivo
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