Home Metabolism Vitamin chemical Thiamine HCl (Vitamin B1)

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Thiamine HCl (Vitamin B1) Vitamin chemical

Cat.No.S3211

Thiamine HCl (Vitamin B1) is a water-soluble vitamin of the B complex, and its phosphate derivatives are involved in many cellular processes.
Thiamine HCl (Vitamin B1) Vitamin chemical Chemical Structure

Chemical Structure

Molecular Weight: 337.27

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Quality Control

Batch: Purity: 99.99%
99.99

Solubility

In vitro
Batch:

Water : 67 mg/mL

DMSO : Insoluble
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : Insoluble

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In vivo
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Chemical Information, Storage & Stability

Molecular Weight 337.27 Formula

C12 H17N4OS.HCl

 Storage (From the date of receipt)
CAS No. 67-03-8 Download SDF Storage of Stock Solutions

Synonyms N/A SMILES CC1=C(SC=[N+]1CC2=CN=C(N=C2N)C)CCO.Cl.[Cl-]

Read more about storage stability stock solution CAS number SMILES

Mechanism of Action

In vitro
Thiamine HCl (Vitamin B1) (50 mM), in addition to its nutritional value, induces systemic acquired resistance (SAR) in rice, tobacco, cucumber, and Arabidopsis. It also confers resistance to fungal, bacterial, and viral infections in treated rice, Arabidopsis (Arabidopsis thaliana), and vegetable crop plants. This compound induces the transient expression of pathogenesis-related (PR) genes in rice and other plants, and potentiates stronger and more rapid PR gene expression along with up-regulation of protein kinase C activity. At a concentration of 10 μM, it prevents acetaldehyde-induced inhibition of myocyte shortening in adult rat ventricular myocytes, effectively blunts the acetaldehyde-induced depression in ±dL/dt, and prevents acetaldehyde-induced shortening of time-to-peak shortening. Furthermore, it inhibits acetaldehyde-induced elevation in both protein carbonyl formation and caspase-3 activation in adult rat ventricular myocytes. Its uptake is energy- and temperature-dependent, pH-sensitive, Na+-independent, and saturable at both the nanomolar (apparent Km, 30 nM) and the micromolar (apparent Km, 1.72 mM) concentration ranges in ARPE-19 cells. Uptake of this compound is adaptively regulated by extracellular substrate level via transcriptionally mediated mechanisms that involve both hTHTR-1 and hTHTR-2 in ARPE-19 cells, and is also regulated by an intracellular Ca2+-calmodulin-mediated pathway. Thiamine-responsive megaloblastic anemia (TRMA) fibroblasts are rescued from death with 10 nM-30 nM of it (in the range of normal plasma thiamine concentrations). Normal fibroblasts exhibit saturable, high-affinity thiamine uptake (Km 400 nM-550 nM; Vmax 11 pmol/min/1×106 cells), while TRMA fibroblasts lack detectable high-affinity uptake. At 30 nM, the rate of its uptake by TRMA fibroblasts is 10-fold less than that of wild-type, which explains the increased apoptosis of TRMA fibroblasts.
In vivo
Thiamine HCl (Vitamin B1) deficiency results in amyloid precursor protein immunoreactivity accumulated in swollen neurites within, or around lesions in rats, or in abnormal clusters in mice.
References
  • [4] https://pubmed.ncbi.nlm.nih.gov/10074490/
  • [5] https://pubmed.ncbi.nlm.nih.gov/9261840/

Applications

Methods Biomarkers Images PMID
Western blot THICL / THICS / THI4L / THI4M / THI4S / ACT Bax / Bid / p53 / p84 Rpb1 / Fcp1 / f-Rpb3 / Tfg3 / Rpb7 / Rpb4
S3211-WB-3
18093957
Growth inhibition assay TEN1 and TEAEN1 strains hippocampal Dcx+ cells
S3211-Growth-inhibition-assay-1
19028995
Glycolysis stress test Glycolysis stress
S3211-Glycolysis-stress-test-1
29914147
Cell death Cell survival
S3211-Cell-death-1
31193162
Western blot / RNA Blot PR-1 / rRNA PR-1 / rRNA
S3211-WB-RNAB-1
15980201
Immunofluorescence Thi76KR / Thi7M399R / ThiN350K
S3211-IF-1
31658248

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2026-08-28)

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT07793071 NOT_YET_RECRUITING
Major Depressive Disorder (MDD); Thiamine; Vitamin B1; Thiamine Diphosphate (TDP); Pediatrics
Massachusetts General Hospital
 2026-10-01 PHASE2
NCT07795996 RECRUITING
Neurocognition, Child
Mount Saint Vincent University
 2026-06-01
NCT06649825 ENROLLING_BY_INVITATION
Familial Adenomatous Polyposis (FAP)
Shanghai Zhongshan Hospital
 2024-01-08
NCT06959251 RECRUITING
Primary Ciliary Dyskinesia
Instituto Nacional de Enfermedades Respiratorias
 2025-10-02
NCT05817851 RECRUITING
Cardiac Arrest
Centre Hospitalier de Bethune
 2023-12-27 PHASE2
NCT07708233 RECRUITING
Diabetic Peripheral Neuropathy (DPN)
Thammasat University
 2026-07-14 PHASE4

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