Thiamine HCl (Vitamin B1)

Thiamine or vitamin B1 is a water-soluble vitamin of the B complex, its phosphate derivatives are involved in many cellular processes.

Thiamine HCl (Vitamin B1) Chemical Structure

Thiamine HCl (Vitamin B1) Chemical Structure

CAS: 67-03-8

Selleck's Thiamine HCl (Vitamin B1) has been cited by 1 publication

Purity & Quality Control

Batch: Purity: 99.99%
99.99

Thiamine HCl (Vitamin B1) Related Products

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Biological Activity

Description Thiamine or vitamin B1 is a water-soluble vitamin of the B complex, its phosphate derivatives are involved in many cellular processes.
In vitro
In vitro Thiamine (50 mM), in addition to its nutritional value, induces systemic acquired resistance (SAR) in rice, tobacco, cucumber, and Arabidopsis. Thiamine-treated rice, Arabidopsis (Arabidopsis thaliana), and vegetable crop plants shows resistance to fungal, bacterial, and viral infections. Thiamine treatment induces the transient expression of pathogenesis-related (PR) genes in rice and other plants. In addition, Thiamine treatment potentiates stronger and more rapid PR gene expression and the up-regulation of protein kinase C activity. [1] Vitamin B1 (10 μM) prevents acetaldehyde-induced inhibition of myocyte shortening in adult rat ventricular myocytes. Vitamin B1 (10 μM) effectively blunts the acetaldehyde-induced depression in ±dL/dt in adult rat ventricular myocytes. Vitamin B1 (10 μM) prevents acetaldehyde-induced shortening of time-to-peak shortening in adult rat ventricular myocytes. Vitamin B1 (10 μM) prevents acetaldehyde-induced elevation in both protein carbonyl formation and caspase-3 activation in adult rat ventricular myocytes. [2] Thiamine uptake is energy- and temperature-dependent, pH-sensitive, Na+-independent, saturable at both the nanomolar (apparent Km, 30 nM) and the micromolar (apparent Km, 1.72 mM) concentration ranges in ARPE-19 cells. Uptake of Thiamine is adaptively regulated by extracellular substrate level via transcriptionally mediated mechanisms that involve both hTHTR-1 and hTHTR-2 in ARPE-19 cells, it is also regulated by an intracellular Ca2+-calmodulin-mediated pathway. [3] Thiamine-responsive megaloblastic anemia (TRMA) fibroblasts are rescued from death with 10 nM-30 nM Thiamine (in the range of normal plasma thiamine concentrations). Normal fibroblasts exhibits saturable, high-affinity thiamine uptake (Km 400 nM-550 nM; Vmax 11 pmol/min/1×106 cells), while TRMA fibroblasts lacks detectable high-affinity uptake. At 30 nM Thiamine, the rate of uptake of Thiamine by TRMA fibroblasts is 10-fold less than that of wild-type, which explains the increased apoptosis of TRMA fibroblasts. [4]
Experimental Result Images Methods Biomarkers Images PMID
Western blot THICL / THICS / THI4L / THI4M / THI4S / ACT Bax / Bid / p53 / p84 Rpb1 / Fcp1 / f-Rpb3 / Tfg3 / Rpb7 / Rpb4 18093957
Growth inhibition assay TEN1 and TEAEN1 strains hippocampal Dcx+ cells 19028995
Glycolysis stress test Glycolysis stress 29914147
Cell death Cell survival 31193162
Western blot / RNA Blot PR-1 / rRNA PR-1 / rRNA 15980201
Immunofluorescence Thi76KR / Thi7M399R / ThiN350K 31658248
In Vivo
In vivo Thiamine deficiency results in amyloid precursor protein immunoreactivity accumulated in swollen neurites within, or around lesions in rats, or in abnormal clusters in mice. [5]
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT06057597 Recruiting
Obesity|Morbid
Assistance Publique - Hôpitaux de Paris
November 13 2023 Not Applicable
NCT05840718 Not yet recruiting
Septic Shock
Hospital Sao Domingos
September 2023 --
NCT05474105 Recruiting
Ischemic Stroke
Barts & The London NHS Trust|Queen Mary University of London|Nutricia Research
July 11 2023 Not Applicable
NCT04505878 Withdrawn
Primary Graft Dysfunction|Lung Transplant; Complications
University of Wisconsin Madison
April 2023 Phase 2

Chemical Information & Solubility

Molecular Weight 337.27 Formula

C12 H17N4OS.HCl

CAS No. 67-03-8 SDF Download Thiamine HCl (Vitamin B1) SDF
Smiles CC1=C(SC=[N+]1CC2=CN=C(N=C2N)C)CCO.Cl.[Cl-]
Storage (From the date of receipt)

In vitro
Batch:

Water : 67 mg/mL

DMSO : Insoluble ( Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : Insoluble


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In vivo
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