research use only

Tetracycline Bacterial inhibitor

Name: Tetracycline
Brand: Selleck Chemicals
SKU: S4490
Availability: InStock
Rating: 5 (5 reviews)

Cat.No.S4490

Tetracycline (TC) is a broad-spectrum antibiotic that exhibits activity against a wide range of microorganisms including gram-positive and gram-negative bacteria, chlamydiae, mycoplasmas, rickettsiae, and protozoan parasites.

Chemical Structure

Molecular Weight: 444.43

Jump to

Quality Control

Batch: Purity: 99.86%

99.86

Related Targets	Integrase Antibiotics Anti-infection Fungal Antiviral COVID-19 Parasite Reverse Transcriptase HIV HCV Protease
Other Bacterial Inhibitors	BTZ043 Racemate Teicoplanin Pefloxacin Mesylate Ceftiofur Solithromycin 6-Methoxydihydrosanguinarine Proanthocyanidins Flagelin 22 Taurolidine Skatole

Solubility

In vitro
Batch:

DMSO : 89 mg/mL (200.25 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 22 mg/mL

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	4.450mg/ml (10.01mM)	Taking the 1 mL working solution as an example, add 50 μL of 89 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.550mg/ml (1.24mM)	Taking the 1 mL working solution as an example, add 50 μL of 11 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	444.43	Formula	C₂₂H₂₄N₂O₈	Storage (From the date of receipt)	3 years -20°C powder
CAS No.	60-54-8	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	TC			SMILES	CN(C)C1C2CC3C(=C(O)C4=C(C=CC=C4O)C3(C)O)C(=O)C2(O)C(=C(C(N)=O)C1=O)O

Read more about storage stability stock solution CAS number SMILES

Mechanism of Action

In vitro	Tetracycline-induced CK2 expression significantly increases the non–small cell lung cancer (NSCLC) invasion in cells expressing BRMS1 wild-type, but not the S30A mutant.
References	[1] https://pubmed.ncbi.nlm.nih.gov/11381101/ [2] https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4873401/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2024-11-07)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT06509139	ENROLLING_BY_INVITATION	Helicobacter Pylori Infection; Sex; Bismuth Quadruple Therapy	National Cheng-Kung University Hospital	2024-07-09	PHASE4
NCT07144657	NOT_YET_RECRUITING	MALT Lymphoma of Stomach	National Taiwan University Hospital	2025-09-01	PHASE2
NCT07788053	NOT_YET_RECRUITING	Helicobatcer Pylori; First-Line Eradication	Chih-An Shih, MD	2026-09-01
NCT04527055	ENROLLING_BY_INVITATION	Helicobacter Pylori Infection; Dysbiosis; Probiotics	National Cheng-Kung University Hospital	2020-05-06	PHASE4
NCT07542639	NOT_YET_RECRUITING	Pulp Disease, Dental; Pulp Necrosis; Pulp Therapy in Primary Molars; Primary Teeth; Pulpectomy; Endodontics; Dental Care for Children; Treatment Outcome; Quality of Life; Health Services Accessibility	University of Sao Paulo	2026-06-08	EARLY_PHASE1
NCT07757919	NOT_YET_RECRUITING	HELICOBACTER PYLORI INFECTIONS	Qilu Hospital of Shandong University	2026-09-01	PHASE4

Tech Support

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):