Home Microbiology Bacterial inhibitor - Antibiotics inhibitor - Antineoplastic and Immunosuppressive Antibiotics chemical Tetracycline For research use only.

Tetracycline

Synonyms: TC

Tetracycline (TC) is a broad-spectrum antibiotic that exhibits activity against a wide range of microorganisms including gram-positive and gram-negative bacteria, chlamydiae, mycoplasmas, rickettsiae, and protozoan parasites.

Tetracycline Chemical Structure

Tetracycline Chemical Structure

CAS: 60-54-8

Selleck's Tetracycline has been cited by 1 publication

Purity & Quality Control

Batch: Purity: 99.86%
99.86

Tetracycline Related Products

Choose Selective Bacterial Inhibitors

Biological Activity

Description Tetracycline (TC) is a broad-spectrum antibiotic that exhibits activity against a wide range of microorganisms including gram-positive and gram-negative bacteria, chlamydiae, mycoplasmas, rickettsiae, and protozoan parasites.
In vitro
In vitro

Tetracycline-induced CK2 expression significantly increases the non–small cell lung cancer (NSCLC) invasion in cells expressing BRMS1 wild-type, but not the S30A mutant.[2]
Cell Research Cell lines H157 BRMS1 knockdown (KD) cells, H157 stable cells
Concentrations 1 µg/mL
Incubation Time 48 h
Method

H157 BRMS1 knockdown (KD) cells were cotransfected with pcDNA3-luciferase and Tet-on CK2α'. Stable clones were selected by Geneticin (400 µg/mL), Blasticidin (5 µg/mL), and Zeocin (150 µg/mL). Myc/His(6)- CK2α' expression was confirmed by immunoblot after treatment with TCN (1 µg/mL) for 48 h. Then, the H157 BRMS1KD/luciferase/Tet-on CK2α' cells were infected with pBabe-shRNA–resistant FLAG-BRMS1 wild-type, S30A mutant, or empty vector and selected with puromycin (1 µg/mL). Flag-BRMS1 expression was confirmed by Western blot. H157 stable cells were pretreated with or without TCN (1 µg/mL) for 48 h. the inserts were coated with type IV collagen at a concentration of 0.25 mg/ml. The bottom portion of the wells included 1% FBS-media. Cells were allowed to migrate for 6 hours or invade for 22 hours. At the specified time point, inserts were fixed and stained with crystal violet.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT06159660 Not yet recruiting
Lung Neoplasm
University of Sydney|National Health and Medical Research Council Australia|Northern Sydney and Central Coast Area Health Service
 March 1 2024 --
NCT06005610 Not yet recruiting
HIV I Infection
National Institute of Allergy and Infectious Diseases (NIAID)
 January 2 2024 Phase 2

Chemical Information & Solubility

Molecular Weight 444.43 Formula

C22H24N2O8
CAS No. 60-54-8 SDF --
Smiles CN(C)C1C2CC3C(=C(O)C4=C(C=CC=C4O)C3(C)O)C(=O)C2(O)C(=C(C(N)=O)C1=O)O
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 89 mg/mL ( (200.25 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 22 mg/mL

Water : Insoluble


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In vivo
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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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