research use only

Sapropterin (Tetrahydrobiopterin) Dihydrochloride Hydroxylase activator

Name: Sapropterin (Tetrahydrobiopterin) Dihydrochloride
Brand: Selleck Chemicals
SKU: S9568
Availability: InStock
Rating: 5 (1 reviews)

Cat.No.S9568

Sapropterin (Tetrahydrobiopterin) Dihydrochloride, a synthetic preparation of the dihydrochloride salt of naturally occurring tetrahydrobiopterin (BH4), is a phenylalanine hydroxylase activator.

Sapropterin (Tetrahydrobiopterin) Dihydrochloride Hydroxylase activator Chemical Structure

Quality Control

Batch: S956802 DMSO]100 mg/mL]false]Water]100 mg/mL]false]Ethanol]˂1 mg/mL]false Purity: 99.82%

Cited in Nature Medicine for its top-tier quality
COA
SDS/MSDS
Datasheet

99.82

Related Targets	Dehydrogenase HSP Transferase P450 (e.g. CYP17) PDE phosphatase PPAR Vitamin Carbohydrate Metabolism Mitochondrial Metabolism
Other Hydroxylase Inhibitors	Ro 61-8048 Meldonium 4-Chloro-DL-phenylalanine LP-533401 (R)-Nepicastat HCl Nepicastat (SYN-117) HCl H-Tyr(3-I)-OH 2-Thiouracil 1,4-DPCA

Solubility

In vitro
Batch:

DMSO : 100 mg/mL (318.29 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : 100 mg/mL

Ethanol : ˂1 mg/mL

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	5.000mg/ml (15.91mM)	Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.500mg/ml (1.59mM)	Taking the 1 mL working solution as an example, add 50 μL of 10 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	314.17	Formula	C₉H₁₇Cl₂N₅O₃	Storage (From the date of receipt)	3 years -20°C powder
CAS No.	69056-38-8	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	R-THBP Dihydrochloride			SMILES	CC(C(C1CNC2=C(N1)C(=O)NC(=N2)N)O)O.Cl.Cl

Read more about storage stability stock solution CAS number SMILES

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2026-05-07)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT07574034	NOT_YET_RECRUITING	Multisystemic Smooth Muscle Dysfunction Syndrome	Gregor Andelfinger	2026-06-01
NCT07255599	ACTIVE_NOT_RECRUITING	Phenylketonuria (PKU)	Sohag University	2025-09-01	PHASE1
NCT06987773	COMPLETED	Bioequivalence	APR Applied Pharma Research s.a.	2025-08-20	PHASE1
NCT06396637	UNKNOWN	Pancreatic Adenocarcinoma Metastatic	Sun Yat-sen University	2024-04-30	PHASE2
NCT06481709	COMPLETED	Bioavailability	APR Applied Pharma Research s.a.	2024-07-30	PHASE1
NCT01965912	COMPLETED	Phenylketonuria	BioMarin Pharmaceutical	2013-10	PHASE4

Tech Support

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):