Home Immunology & Inflammation ROS inhibitor (S)-Methylisothiourea sulfate For research use only.

(S)-Methylisothiourea sulfate

(S)-Methylisothiourea sulfate is a potent inhibitor of inducible NO synthase (iNOS).

(S)-Methylisothiourea sulfate Chemical Structure

(S)-Methylisothiourea sulfate Chemical Structure

CAS: 867-44-7

Selleck's (S)-Methylisothiourea sulfate has been cited by 2 publications

Purity & Quality Control

Batch: S363101 Water] 27 mg/mL] false] DMSO] Insoluble] false] Ethanol] Insoluble] false Purity: 99.66%
99.66

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Biological Activity

Description (S)-Methylisothiourea sulfate is a potent inhibitor of inducible NO synthase (iNOS).
Targets
iNOS [1]
In vitro
In vitro S-Methylisothiourea sulfate (SMT) is at least 10- to 30-fold more potent as an inhibitor of inducible NOS (iNOS) in immunostimulated cultured macrophages (EC50, 6 microM) and vascular smooth muscle cells (EC50, 2 microM) than NG-methyl-L-arginine (MeArg) or any other NOS inhibitor yet known. The effect of SMT on iNOS activity can be reversed by excess L-arginine in a concentration-dependent manner. SMT is a competitive inhibitor of iNOS activity at the L-arginine site. SMT (up to 1 mM) does not inhibit the activity of xanthine oxidase, diaphorase, lactate dehydrogenase, monoamine oxidase, catalase, cytochrome P450, or superoxide dismutase. SMT is equipotent with MeArg in inhibiting the endothelial, constitutive isoform of NOS in vitro[2].
Cell Research Cell lines J774.2 macrophages
Concentrations 10 nM-0.1 M
Incubation Time 24 h
Method --
In Vivo
In vivo Treatment of the mice with S-methylisothiourea sulfate (SMT), a potent inhibitor of inducible NO synthase (NOS), prevents the elevation of serum NO levels and concomitantly reduces the enhanced response to pentylenetetrazole[1]. Therapeutic administration of SMT (5 mg/kg, i.p., given 2 hr after LPS, 10 mg/kg, i.p.) attenuates the rises in plasma alanine and aspartate aminotransferases, bilirubin, and creatinine and also prevents hypocalcaemia when measured 6 hr after administration of LPS. SMT (1 mg/kg, i.p.) improves 24-hr survival of mice treated with a high dose of LPS (60 mg/kg, i.p.)[2]. SMT is beneficial to myocardial contractility in this model of endotoxemia[3].
Animal Research Animal Models ICR mice
Dosages 2 mg/kg
Administration i.p.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT05770284 Not yet recruiting
Liver Diseases
Institute of Liver and Biliary Sciences India
 March 15 2023 Not Applicable
NCT02858362 Terminated
Duchenne Muscular Dystrophy
Summit Therapeutics
 June 2016 Phase 2

Chemical Information & Solubility

Molecular Weight 139.19 Formula

C2H6N2S.1/2 H2SO4
CAS No. 867-44-7 SDF Download (S)-Methylisothiourea sulfate SDF
Smiles CSC(=N)N.CSC(=N)N.OS(=O)(=O)O
Storage (From the date of receipt)

In vitro
Batch:

Water : 27 mg/mL

DMSO : Insoluble ( Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : Insoluble


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In vivo
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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