Nigericin (NSC 292567) sodium salt

Catalog No.S6653

For research use only.

Nigericin (NSC 292567) sodium salt is an antibiotic derived from Streptomyces hygroscopicus that works by acting as an H+, K+, and Pb2+ ionophore.Nigericin can activate NLRP3 inflammasome to induce pro-inflammatory and immunostimulatory processes.

Nigericin (NSC 292567) sodium salt Chemical Structure

CAS No. 28643-80-3

Selleck's Nigericin (NSC 292567) sodium salt has been cited by 5 Publications

Purity & Quality Control

Choose Selective Antineoplastic and Immunosuppressive Antibiotics Inhibitors

Biological Activity

Description Nigericin (NSC 292567) sodium salt is an antibiotic derived from Streptomyces hygroscopicus that works by acting as an H+, K+, and Pb2+ ionophore.Nigericin can activate NLRP3 inflammasome to induce pro-inflammatory and immunostimulatory processes.
Targets
Potassium channel [1]
()
Hydrogen channel [1]
()
Pb 2+ [1]
()
NLRP3 [2]
()
In vitro

In vitro model of tumor heterogeneity the antitumor effects of Nigericin (NIG) that works by acting as an H+, K+, and Pb2+ ionophore. NIG decreased the viability and clonogenicity of H460 cells, as well as their ability to migrate and invade[1] . Using a combination of live-cell imaging and biochemical approaches, we demonstrate that prototypical NLRP3 stimuli, the potassium ionophore nigericin, or millimolar concentrations of ATP exert both NLRP3-independent and -dependent effects on macrophages that result in cytokine release and cell death. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
H9 M1PQXmFvfGm4aYLhcEBie3OjeR?= NUXtRWxSSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEmYMTDSSkBqdiCqdX3hckBJQSClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJJZqenW|LXnu[JVk\WRiY4n0c5BifGirYzDl[oZm[3RiYomg[o9zdWG8YX6tZoF{\WRiY3;ueoVvfGmxbnHsJINwdG:{aX3leJJq[yC2ZXPocolyfWVuIFXDOVA:OC5yMEdOwG0v M1TwPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzNEOwNFE6Lz5zMUSzNFAyQTxxYU6=
H9 NEDwPIJEgXSxdH;4bYNqfHliYYPzZZk> NFLzTJhEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJQSClZXzsd{whUUN3ME2wMlE1|ryPLh?= M2HN[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzNEOwNFE6Lz5zMUSzNFAyQTxxYU6=

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: H460 cells
  • Concentrations: 1 µM
  • Incubation Time: 24 h
  • Method:

    H460 cells are seeded in 8-µm pore size Transwell® Inserts in RPMI 1640 (without FBS) with or without drugs (DMSO as control) or NIG (1µM). Inserts are placed in 24-well plates with RPMI 1640 containing 10% FBS in the presence of DMSO (control) or NIG (1 µM).

  • (Only for Reference)

Solubility (25°C)

In vitro

Ethanol 100 mg/mL
(133.87 mM)
DMSO Insoluble
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 746.94
Formula

C40H67NaO11

CAS No. 28643-80-3
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC1CCC(OC1C(C)C(=O)[O-])CC2CC(C(C3(O2)C(CC(O3)(C)C4CCC(O4)(C)C5C(CC(O5)C6C(CC(C(O6)(CO)O)C)C)C)C)C)OC.[Na+]

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Nigericin (NSC 292567) sodium salt | Nigericin (NSC 292567) sodium salt supplier | purchase Nigericin (NSC 292567) sodium salt | Nigericin (NSC 292567) sodium salt cost | Nigericin (NSC 292567) sodium salt manufacturer | order Nigericin (NSC 292567) sodium salt | Nigericin (NSC 292567) sodium salt distributor