Nigericin sodium salt

For research use only.

Catalog No.S6653

Nigericin sodium salt Chemical Structure

Molecular Weight(MW): 746.94

Nigericin sodium salt is an antibiotic derived from Streptomyces hygroscopicus that works by acting as an H+, K+, and Pb2+ ionophore.Nigericin can activate NLRP3 inflammasome to induce pro-inflammatory and immunostimulatory processes.

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Biological Activity

Description Nigericin sodium salt is an antibiotic derived from Streptomyces hygroscopicus that works by acting as an H+, K+, and Pb2+ ionophore.Nigericin can activate NLRP3 inflammasome to induce pro-inflammatory and immunostimulatory processes.
Targets
Potassium channel [1]
()
Hydrogen channel [1]
()
Pb 2+ [1]
()
NLRP3 [2]
()
In vitro

In vitro model of tumor heterogeneity the antitumor effects of Nigericin (NIG) that works by acting as an H+, K+, and Pb2+ ionophore. NIG decreased the viability and clonogenicity of H460 cells, as well as their ability to migrate and invade[1] . Using a combination of live-cell imaging and biochemical approaches, we demonstrate that prototypical NLRP3 stimuli, the potassium ionophore nigericin, or millimolar concentrations of ATP exert both NLRP3-independent and -dependent effects on macrophages that result in cytokine release and cell death. [2]

Protocol

Cell Research:

[1]

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  • Cell lines: H460 cells
  • Concentrations: 1 µM
  • Incubation Time: 24 h
  • Method:

    H460 cells are seeded in 8-µm pore size Transwell® Inserts in RPMI 1640 (without FBS) with or without drugs (DMSO as control) or NIG (1µM). Inserts are placed in 24-well plates with RPMI 1640 containing 10% FBS in the presence of DMSO (control) or NIG (1 µM).


    (Only for Reference)

Solubility (25°C)

In vitro Ethanol 100 mg/mL (133.87 mM)
DMSO Insoluble
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 746.94
Formula

C40H67NaO11

CAS No. 28643-80-3
Storage powder
in solvent
Synonyms N/A

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID