Oleic Acid

Technical Data

Biological Activity

Protocol (from reference)

References

Selleck's Oleic Acid has been cited by 6 publications

Catalog No.S4707 Batch:S470702

Formula	C₁₈H₃₄O₂
Molecular Weight	282.46	CAS No.	112-80-1
Solubility (25°C)*	In vitro


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Cell Assay:^{^[1]}	Cell lines Human RCC（renal cell carcinoma） cell line, 786-O. Concentrations 0.05, 0.1, 0.2 mmol/l Incubation Time 48 h Method Cells (2x10³ cells/well) are seeded in 96-well microtitre plates and incubated for 24 h in 100 µl culture medium. Cells in the experimental group were then treated with 0.05, 0.1 or 0.2 mmol/l oleic acid for 48 h. MTT [20 µl (5 g/l)] is added to the cells which were then cultivated for a further 4 h. Following the removal of the supernatant fluid, 150 µl/well DMSO is added to the cells which are agitated for 15 min. Absorbance is measured at 490 nm by an ELISA reader.
Animal Study:^{^[3]}	Animal Models male Swiss mice Dosages 2.5, 5.0 or 10.0 μmol Administration i.v.

Cell Assay:^{^[1]}

Cell lines
Human RCC（renal cell carcinoma） cell line, 786-O.
Concentrations
0.05, 0.1, 0.2 mmol/l
Incubation Time
48 h
Method
Cells (2x10³ cells/well) are seeded in 96-well microtitre plates and incubated for 24 h in 100 µl culture medium. Cells in the experimental group were then treated with 0.05, 0.1 or 0.2 mmol/l oleic acid for 48 h. MTT [20 µl (5 g/l)] is added to the cells which were then cultivated for a further 4 h. Following the removal of the supernatant fluid, 150 µl/well DMSO is added to the cells which are agitated for 15 min. Absorbance is measured at 490 nm by an ELISA reader.

Animal Study:^{^[3]}

Animal Models
male Swiss mice
Dosages
2.5, 5.0 or 10.0 μmol
Administration
i.v.

[4] Amtul Z, et al. Brain Pathol. 2011, 21(3):321-9.

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Description

Oleic acid (Oleate, 9-Octadecenoic Acid, Elaidoic acid) is a fatty acid that occurs naturally in various animal and vegetable fats and oils. It is used commercially in the preparation of oleates and lotions, and as a pharmaceutical solvent. Oleic acid is an abundant monounsaturated fatty acid. Oleic acid is a Na+/K+ ATPase activator.

Oleic acid treatment induces a marked increase in the proliferation of 786-O cells in a dose-dependent manner. It delays 786-O cell apoptosis. In 786‑O cells, ILK is upregulated by oleic acid treatment. Oleic acid activates G protein-coupled receptors and phosphorylates ERK1/2 to induce cancer cell proliferation in breast cancer^[1]. Oleic acid(as low as 5 M) inhibits the stearic acid-induced increase in intercellular adhesion molecule-1 (ICAM-1) expression. Oleic acid is able to diminish endothelial cell activation, irrespective of the stimulus utilized, by suppressing the upregulation of adhesion molecules. It can protect Chinese hamster ovary cells from palmitate-induced lipotoxicity by promoting triglyceride synthesis within the cells^[2].

Oleic acid inhibits in vivo Na/K-ATPase activity in the lungs and induced lung injury. 10 μM oleic acid induces lung edema and inflammation with cell influx, lipid body formation, and leukotriene B4 (LTB4) and prostaglandin E2 (PGE2) production^[3]. Oleic acid ameliorates amyloidosis in mouse models of Alzheimer's disease^[4].

0.89 g/mL at 25 °C

Targeting neoadjuvant chemotherapy-induced metabolic reprogramming in pancreatic cancer promotes anti-tumor immunity and chemo-response [ Cell Rep Med, 2023, 4(10):101234]	PubMed: 37852179
SLC25A22 as a Key Mitochondrial Transporter Against Ferroptosis by Producing Glutathione and Monounsaturated Fatty Acids [ Antioxid Redox Signal, 2023, 39(1-3):166-185.]	PubMed: 37051693
SIRT1 prevents cigarette smoking-induced lung fibroblasts activation by regulating mitochondrial oxidative stress and lipid metabolism [ J Transl Med, 2022, 20(1):222]	PubMed: 35568871
FGF9 Alleviates the Fatty Liver Phenotype by Regulating Hepatic Lipid Metabolism [ Front Pharmacol, 2022, 13:850128]	PubMed: 35517790
MEN1 promotes ferroptosis by inhibiting mTOR-SCD1 axis in pancreatic neuroendocrine tumors [ Acta Biochim Biophys Sin (Shanghai), 2022, 54(11):1599-1609]	PubMed: 36604142
Ethyl 2-[2,3,4-Trimethoxy-6-(1-Octanoyl)Phenyl] Acetate (TMPA) Ameliorates Lipid Accumulation by Disturbing the Combination of LKB1 with Nur77 and Activating the AMPK Pathway in HepG2 Cells and Mice Primary Hepatocytes [ Diabetes Metab Syndr Obes, 2021, 14:4165-4177]	PubMed: 34629883

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