Midostaurin (PKC412)

Catalog No.S8064 Synonyms: CGP 41251

For research use only.

Midostaurin (pkc412, CGP 41251) is a multi-targeted kinase inhibitor, including PKCα/β/γ, Syk, Flk-1, Akt, PKA, c-Kit, c-Fgr, c-Src, FLT3, PDFRβ and VEGFR1/2 with IC50 ranging from 80-500 nM.

Midostaurin (PKC412) Chemical Structure

CAS No. 120685-11-2

Selleck's Midostaurin (PKC412) has been cited by 32 publications

Purity & Quality Control

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Biological Activity

Description Midostaurin (pkc412, CGP 41251) is a multi-targeted kinase inhibitor, including PKCα/β/γ, Syk, Flk-1, Akt, PKA, c-Kit, c-Fgr, c-Src, FLT3, PDFRβ and VEGFR1/2 with IC50 ranging from 80-500 nM.
Targets
PKCα [1]
(Cell-free assay)
PKCγ [1]
(Cell-free assay)
PKCβ1 [1]
(Cell-free assay)
PKCβ2 [1]
(Cell-free assay)
PPK [1]
(Cell-free assay)
Click to View More Targets
22 nM 24 nM 30 nM 31 nM 38 nM
In vitro

Midostaurin(pkc412) is a broad spectrum protein kinase inhibitor. Midostaurin(pkc412) interacts strongly with ATP binding sites of the conventional PKC-α, -β and -γ, PDGFRβ, VEGF-R2, VEGF-R1 and the cyclin-dependant kinase 1-cyclin B complex. Midostaurin(pkc412) inhibits the growth of various human and animal cell lines in vitro at similar submicromolar concentrations. Midostaurin(pkc412) also effectively inhibits the in vitro proliferation of glioblastoma and induced the accumulation of cells in G2/M and formation of giant nuclei with extensive fragmentation and apoptotic bodies. Midostaurin(pkc412) is able to reverse the p-glycoprotein-mediated multidrug resistance of tumor cells in vitro. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
EOL-1 NUjoNpZbS3m2b4TvfIlkcXS7IHHzd4F6 M3fmb|czKGi{cx?= MVrDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDFU2wuOSClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRUKGG|c3H5MEBKSzVyIE2gNE4xODN6IN88UU4> MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTN3MEmyNEc,Ojl|NUC5NlA9N2F-
MOLM13 M{[3fmN6fG:2b4jpZ4l1gSCjc4PhfS=> NXL1[XZsPzJiaILz M{TGO2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1QVE1zMzDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSVIHHzd4F6NCCLQ{WwJF0hOC5yMES3JO69VS5? MlT5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl|NUC5NlAoRjJ7M{WwPVIxRC:jPh?=
MV4-11 NV3WVYloS3m2b4TvfIlkcXS7IHHzd4F6 MUC3NkBpenN? M4TTd2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1XPC1zMTDj[YxteyCjZoTldkA4OiCqcoOgZpkh[2WubDD0bZRmei2kbIXlJIF{e2G7LDDJR|UxKD1iMD6wNVIh|ryPLh?= MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTZ3NESwPEc,OTl4NUS0NFg9N2F-
RS4-11 MVTGeY5kfGmxbjDhd5NigQ>? MnHsNkBpenN? M3Ljd2lvcGmkaYTpc44hd2ZiRlzUN{BKXERibYX0ZY51KGG3dH;wbI9{eGixconsZZRqd25iaX6gbJVu[W5iUmO0MVEyKGOnbHzzJIFnfGW{IEKgbJJ{KGK7IHXs[YN1em:laHXtbYx2dWmwZYPj[Y5k\SCjc4PhfUwhUUN3MDC9JFAvODF|IN88UU4> NGXqN209[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOU[1OFQxQCd-MUm2OVQ1ODh:L3G+
RS4-11 NGDNTndHfW6ldHnvckBie3OjeR?= MnXDNkBpenN? NIXYbo9KdmirYnn0bY9vKG:oIF\MWFMh[XW2b4Doc5NxcG:{eXzheIlwdiCrbjDoeY1idiCUU{StNVEh[2WubIOgZYZ1\XJiMjDodpMh[nliZXzlZ5Rzd2OqZX3pcJVucW6nc3PlcoNmKGG|c3H5MEBKSzVyIE2gNE4xOTVizszNMi=> M1PvU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7NkW0OFA5Lz5zOU[1OFQxQDxxYU6=
Sf9 Mn25SpVv[3Srb36gZZN{[Xl? MoXsOEBpenN? MYfJcohq[mm2aX;uJI9nKHerbHSgeJlx\SCJU2SteIFo\2WmIF\MWFMhc2mwYYPlJIRwdWGrbjCoXVU3PyC2bzDTPVk{MSBqdX7rco94diCxcnnnbY4qKGW6cILld5Nm\CCrbjDiZYN2dG:4aYL1d{Bqdm[nY4Tl[EBqdnOnY4SgV4Y6KGOnbHzzJJV{cW6pIFjldlIheGWydHnk[UBieyC|dXLzeJJifGViYX\0[ZIhPCCqcoOgZpkhU2mwYYPlMWdtdyCjc4PhfUwhUUN3MDC9JFAvODN5IN88UU4> M1zUelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MEixNFI{Lz5{NkC4NVAzOzxxYU6=
MV4-11 M3m2UWZ2dmO2aX;uJIF{e2G7 MoDEO|IhcHK| NELJUnFKdmirYnn0bY9vKG:oIF\MWFMhUVSGIHjvcY97gWexdYOgcZV1[W62IHnuJIh2dWGwIF3WOE0yOSClZXzsd{Bie3Onc4Pl[EBieyClZXzsJIdzd3e2aDDpcohq[mm2aX;uJIFnfGW{IEeyJIhzeyCkeTDNWHMh[XO|YYmsJGdKPTBiPTCwMlA{QCEQvF2u NE\id2s9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkC4NVAzOyd-Mk[wPFExOjN:L3G+
MOLM-13 MWHGeY5kfGmxbjDhd5NigQ>? MU[3NkBpenN? MV\Jcohq[mm2aX;uJI9nKE[OVEOgTXRFKGindHXyc5p6\2:3czDteZRidnRiaX6gbJVu[W5iTV;MUU0yOyClZXzsd{Bie3Onc4Pl[EBieyClZXzsJIdzd3e2aDDpcohq[mm2aX;uJIFnfGW{IEeyJIhzeyCkeTDNWHMh[XO|YYmsJGdKPTBiPTCwMlA2PSEQvF2u M2fjV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MEixNFI{Lz5{NkC4NVAzOzxxYU6=
Sf9 Mn;iSpVv[3Srb36gZZN{[Xl? NFPORoE6OCCvaX7z MXTJcohq[mm2aX;uJI9nKHKnY3;tZolv[W62IFfTWE11[WepZXSgRZVzd3KjLVGgb4lv[XOnIHTvcYFqdiBqU{GyN{B1dyCVNECxLUApfW6tbn;3ckBwemmpaX6pJIV5eHKnc4Pl[EBqdiCrboPlZ5QhW2Z7IHPlcIx{KHW|aX7nJJRmfHKjKFzSVmFUVEdrIIDldJRq\GViYYOgd5Vje3S{YYTlJIFnfGW{IEmwJI1qdnNiYomgT4lv[XOnLVfsc{Bie3OjeTygTWM2OCB;IECuNFgh|ryPLh?= MoCzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZyOEGwNlMoRjJ4MEixNFI{RC:jPh?=
FDC-P1 NH3YdWhHfW6ldHnvckBie3OjeR?= NVX5epE{PDhiaILz MVnJcohq[mm2aX;uJI9nKGi3bXHuJGZOWyCneIDy[ZN{\WRiaX6g[5Jwf3SqIH\hZ5RweiCmZYDlcoRmdnRibX;1d4UhTkSFLWCxJINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiRl3TMY1m\GmjdHXkJINmdGxicILvcIln\XKjdHnvckBqdiCycnXz[Y5k\SCqdX3hckBEW0ZzIHHmeIVzKDR6IHjyd{BjgSC{ZYPhfpVzcW5iZInlJJJm\HWldHnvckBie3OjeTygTWM2OCB;IECuNVQzKM7:TT6= Mny1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjBzNU[2PFkoRjJyMUW2Olg6RC:jPh?=
A375 M37HXnRwgGmlaYT5JIF{e2G7 NWrKXWwzPzJiaILz MmD6WI95cWOrdImgZYdicW6|dDDoeY1idiCDM{e1JINmdGy|IHHmeIVzKDd{IHjyd{BjgSClZXzsJJRqfGW{LXLseYUh[XO|YYmsJGlEPTBiPTCwMlE5KM7:TT6= NYnjbohsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm2OVQ1ODhpPkG5OlU1PDB6PD;hQi=>
FDC-P1 MnTlSpVv[3Srb36gZZN{[Xl? NEHDTJg1QCCqcoO= NVXJT|YzUW6qaXLpeIlwdiCxZjDtc5V{\SCJTT3DV2Yue3SrbYXsZZRm\CClZXzsJJBzd2yrZnXyZZRqd25ib3[g[5Jwf3SqIH\hZ5RweiCmZYDlcoRmdnRibX;1d4UhTkSFLWCxJINmdGy|IHX4dJJme3OrbnegbJVu[W5iRl3TJIFnfGW{IES4JIhzeyCkeTDy[ZNignW{aX6g[JlmKHKnZIXjeIlwdiCjc4PhfUwhUUN3MDC9JFAvOiEQvF2u NFGxZmE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEG1OlY5QSd-MkCxOVY3QDl:L3G+
BAF3 M1HydmFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NFvSfVhCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JI1wfXOnIFLBSlMh[2WubIOgeJJidnOob4Lt[YQhf2m2aDDaUmYyQThvRlfGVlEh[2:wc4TyeYN1NCCLQ{WwJF0hOC5{IN88UU4> NVrVRm9qRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG5N|Y2PDJpPkKxPVM3PTR{PD;hQi=>
Sf9 MoroSpVv[3Srb36gZZN{[Xl? MV[xNlAhdWmwcx?= NXr6b2s4UW6qaXLpeIlwdiCxZjDy[YNwdWKrbnHueEBIW1RvdHHn[4VlKF[HR1\SNkBscW6jc3Wg[I9u[WmwIDjWO|g6KHSxIG[xN|U3MSBqdX7rco94diCxcnnnbY4qKGW6cILld5Nm\CCrbjDpcpNm[3RiU3[5JINmdGy|IIXzbY5oKHCxbInHcJU1QlS7cjDw[ZB1cWSnIHHzJJN2[nO2cnH0[UBi\nSncjCxNlAhdWmwczDifUBMcW6jc3WtS4xwKGG|c3H5MEBKSzVyIE2gNE4zPSEQvF2u MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjB6MUCyN{c,OjZyOEGwNlM9N2F-
RS4:11 M3XjZ2Z2dmO2aX;uJIF{e2G7 M2WxTlczKGi{cx?= MX7Jcohq[mm2aX;uJI9nKHerbHSgeJlx\SCqb33vfplod3W|IF\MWFMhcW5iaIXtZY4hWlN2OkGxJINmdGy|IHHzd4V{e2WmIHHzJINmdGxiZ4Lve5RpKGmwaHnibZRqd25iYX\0[ZIhPzJiaILzJIJ6KE2WUzDhd5NigSxiR1m1NEA:KDBwNDFOwG0v NX\ZdWJQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wPFExOjNpPkK2NFgyODJ|PD;hQi=>
U937 MlnER5l1d3SxeHnjbZR6KGG|c3H5 M1rlWVczKGi{cx?= NHTuRodEgXSxdH;4bYNqfHliYXfhbY5{fCCITGSzMYRm\mmlaXXueEBpfW2jbjDVPVM4KGOnbHzzJIF{e2W|c3XkJIF{KGOnbHyg[5Jwf3SqIHnubIljcXSrb36gZYZ1\XJiN{KgbJJ{KGK7IF3UV{Bie3OjeTygS2k2OCB;IEGuOEDPxE1w M2q0NlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MEixNFI{Lz5{NkC4NVAzOzxxYU6=
SJ-GBM2 NFL5PHZyUFSVIHHzd4F6 MWXxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhW0pvR1LNNkBk\Wyucx?= MknCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
A673 M{Xi[ZFJXFNiYYPzZZk> M2\PSZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCDNkezJINmdGy| M33PR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
SK-N-MC MlTzdWhVWyCjc4PhfS=> M2rzd5FJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCVSz3OMW1EKGOnbHzz NFjq[lc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
U-2 OS MoW0dWhVWyCjc4PhfS=> MVrxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhXS1{IF;TJINmdGy| NV7rbHg4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
Saos-2 MUDxTHRUKGG|c3H5 M2nrS5FJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCVYX;zMVIh[2WubIO= MmjTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
SK-N-SH MWPxTHRUKGG|c3H5 NVXrS3JUeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGPLMW4uW0hiY3XscJM> NEHheHc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
LAN-5 NYH4fXFweUiWUzDhd5NigQ>? NFG2N29yUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiTFHOMVUh[2WubIO= M1nxfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
Rh18 MXvxTHRUKGG|c3H5 NEPqWoJyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiUnixPEBk\Wyucx?= M3T6[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
OHS-50 MnHBdWhVWyCjc4PhfS=> MV3xTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhV0iVLUWwJINmdGy| NV;tOFNbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
MG 63 (6-TG R) MnzsdWhVWyCjc4PhfS=> MWTxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhVUdiNkOgLFYuXEdiUjmgZ4VtdHN? NHWzW2g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
NB1643 MkDKdWhVWyCjc4PhfS=> M3Hoe5FJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJG5DOTZ2MzDj[Yxtew>? M2e4NFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
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NCI-H748 Mof5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVHJcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KN{S4JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUCgQUA5NjB4N{e4JO69VS5? NX\Ub|dsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPjB6NUOzM{c,W0GQR1XSQE9iRg>?
LB771-HNC MnjyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnHjTY5pcWKrdHnvckBw\iCqdX3hckBNSjd5MT3IUmMh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NEA:KDhwMUO4N{DPxE1w MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly2NFg2OzNxJ{7TRW5ITVJ:L3G+
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SJSA-1 M2fvPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUnJcohq[mm2aX;uJI9nKGi3bXHuJHNLW0FvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyIE2gPE41ODN4MzFOwG0v NYL3OpJTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPjB6NUOzM{c,W0GQR1XSQE9iRg>?
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ATN-1 MnHaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEnzVWlKdmirYnn0bY9vKG:oIHj1cYFvKEGWTj2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUCgQUA5NjZ3Mkm1JO69VS5? NWq5fFB6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPjB6NUOzM{c,W0GQR1XSQE9iRg>?
HAL-01 NWf1VGttT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXHOUlM1UW6qaXLpeIlwdiCxZjDoeY1idiCKQVytNFEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NEA:KDhwN{S0NVMh|ryPLh?= NYXFToZIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPjB6NUOzM{c,W0GQR1XSQE9iRg>?
LU-139 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX3Jcohq[mm2aX;uJI9nKGi3bXHuJGxWNTF|OTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyIE2gPE46OjZzNTFOwG0v NGXwVW09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw3ODh3M{OvK|5USU6JRWK8M4E,
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NCI-H1694 NW\xeXRtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXTrOGxMUW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFE3QTRiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OCB;IEmuN|czPTNizszNMi=> MnfTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOlA5PTN|Lze+V2FPT0WUPD;hQi=>
COR-L88 MnzvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVHJcohq[mm2aX;uJI9nKGi3bXHuJGNQWi2OOEigZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3MDC9JFkvPjByNUWg{txONg>? NV\QdFFFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPjB6NUOzM{c,W0GQR1XSQE9iRg>?
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KM-H2 NX3FOIl2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUfJcohq[mm2aX;uJI9nKGi3bXHuJGtONUh{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTBiPTC5Mlc3OTlizszNMi=> M2qwTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFYxQDV|Mz:nQnNCVkeHUkyvZV4>
NCI-H1092 M2TXbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYXJcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KMUC5NkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwJF0hQS55OUK1N{DPxE1w MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly2NFg2OzNxJ{7TRW5ITVJ:L3G+
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MHH-CALL-2 NGDjZZRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NImzbYNKdmirYnn0bY9vKG:oIHj1cYFvKE2KSD3DRWxNNTJiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OCB;IEGwMlAyPjlizszNMi=> M{\zOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFYxQDV|Mz:nQnNCVkeHUkyvZV4>
NCI-SNU-5 NX7uS|ZbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1zs[GlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLWPOWU02KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVAhRSBzMD6zNlQzKM7:TT6= MmDJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOlA5PTN|Lze+V2FPT0WUPD;hQi=>
BPH-1 MoOxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGDEVGpKdmirYnn0bY9vKG:oIHj1cYFvKEKSSD2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUCgQUAyOC5|N{e3JO69VS5? MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly2NFg2OzNxJ{7TRW5ITVJ:L3G+
P12-ICHIKAWA M3r2Rmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFXPSpVKdmirYnn0bY9vKG:oIHj1cYFvKFBzMj3JR2hKU0GZQTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyIE2gNVEvOTB7NTFOwG0v NYDqSIkyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPjB6NUOzM{c,W0GQR1XSQE9iRg>?
NCI-H727 NGf0eVNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1zJemlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLVi3Nlch[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NEA:KDFzLkGzPVIh|ryPLh?= M{freVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFYxQDV|Mz:nQnNCVkeHUkyvZV4>
COR-L23 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGjnbmFKdmirYnn0bY9vKG:oIHj1cYFvKEORUj3MNlMh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NEA:KDFzLkG4O|gh|ryPLh?= MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly2NFg2OzNxJ{7TRW5ITVJ:L3G+
U-118-MG M2[1Tmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYPJcohq[mm2aX;uJI9nKGi3bXHuJHUuOTF6LV3HJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUCgQUAyOS5{N{e5JO69VS5? MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly2NFg2OzNxJ{7TRW5ITVJ:L3G+
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COLO-829 MlPnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWiye5kzUW6qaXLpeIlwdiCxZjDoeY1idiCFT1zPMVgzQSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxKD1iMUGuOlE6OiEQvF2u MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly2NFg2OzNxJ{7TRW5ITVJ:L3G+
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THP-1 NWO2enl5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml[4TY5pcWKrdHnvckBw\iCqdX3hckBVUFBvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyIE2gNVIvODd2ODFOwG0v MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly2NFg2OzNxJ{7TRW5ITVJ:L3G+
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647-V M4DuTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NELhc2tKdmirYnn0bY9vKG:oIHj1cYFvKDZ2Nz3WJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUCgQUAyOi5yOUeyJO69VS5? NYLne2pxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPjB6NUOzM{c,W0GQR1XSQE9iRg>?
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DOHH-2 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3rzN2lvcGmkaYTpc44hd2ZiaIXtZY4hTE:KSD2yJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUCgQUA{QC57M{WxJO69VS5? NYHN[G1iRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPjB6NUOzM{c,W0GQR1XSQE9iRg>?
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UACC-893 M3fRUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHW2[mNKdmirYnn0bY9vKG:oIHj1cYFvKFWDQ1OtPFk{KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVAhRSB2Nz6zO|M3KM7:TT6= NUfPTJVNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPjB6NUOzM{c,W0GQR1XSQE9iRg>?
Assay
Methods Test Index PMID
Western blot PKCα / PKCβ1 / PKCβ2 / PKCγ / PKCη / p-PKC / p-Bad / Bad / p-Bcl2 / Bcl-2 / p-p65 / p65 ; p-TYR / p-STAT5 / STAT5 / p-S6 / S6 / p-MAPK / MAPK / p-AKT / AKT 30584254 28881711
In vivo Midostaurin(pkc412) may suppress tumor growth by inhibiting tumor angiogenesis (via its effects on the VEGF receptor tyrosine kinases) in addition to directly inhibiting tumor cell proliferation (via its effects on PKCs). This anti-angiogenic action may contribute to the antimetastatic and broad antitumor activity displayed by midostaurin(pkc412), as well as the synergy with cytotoxic agents, including doxorubicin, cyclophosphamide, cisplatin and gemcitabine. When given orally, the maximally tolerated dose for midostaurin(pkc412) is >300 mg/kg. [1]

Protocol (from reference)

Cell Research:[2]
  • Cell lines: A549, NCI-H520
  • Concentrations: ~1.0 μM
  • Incubation Time: 24-72 h
  • Method: Each well is added with 5 mM WST-1 and 0.2 mM 1-methoxy PMS and the absorbance at 450 nm is measured by a Microplate Reader.
Animal Research:[1]
  • Animal Models: Colo 205 colorectal tumors xenograft
  • Dosages: 50 mg/kg, 200 mg/kg, once daily
  • Administration: p.o.

Solubility (25°C)

In vitro

DMSO 100 mg/mL
(175.24 mM)
Water Insoluble
Ethanol ''''''20 mg/mL warmed

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
5% DMSO+45% PEG 300+ddH2O
For best results, use promptly after mixing.

4mg/mL

Chemical Information

Molecular Weight 570.64
Formula

C35H30N4O4

CAS No. 120685-11-2
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC12C(C(CC(O1)N3C4=CC=CC=C4C5=C6C(=C7C8=CC=CC=C8N2C7=C53)CNC6=O)N(C)C(=O)C9=CC=CC=C9)OC

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03951961 Terminated Drug: Midostaurin Acute Myeloid Leukemia Adult University of Jena|Ludwig-Maximilians - University of Munich March 20 2020 Phase 2
NCT04097470 Active not recruiting Drug: Decitabine|Drug: Midostaurin AML/MDS Stichting Hemato-Oncologie voor Volwassenen Nederland|Swiss Group for Clinical Cancer Research December 5 2019 Phase 2

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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